Restriction map for
pFN21AE2514
(Linear) (Six-base) MAPSORT of: KIEE2514.seq Check: 7398 from: 1 to: 2682
KIEE2514.orf
With 182 enzymes: SgfI *
March 20, 2014 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 2682
Size: 386 2296
AatII G_ACGT'C
Cuts at: 0 2657 2682
Size: 2657 25
AccI GT'mk_AC
Cuts at: 0 2594 2682
Size: 2594 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1650 1891 2571 2682
Size: 933 717 241 680 111
Fragments arranged by size:
933 717 680 241 111
AflIII A'CryG_T
Cuts at: 0 1385 2117 2387 2492 2682
Size: 1385 732 270 105 190
Fragments arranged by size:
1385 732 270 190 105
AleI CACnn'nnGTG
Cuts at: 0 1025 2682
Size: 1025 1657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 1043 1177 2682
Size: 447 596 134 1505
Fragments arranged by size:
1505 596 447 134
AlwNI CAG_nnn'CTG
Cuts at: 0 64 2133 2682
Size: 64 2069 549
Fragments arranged by size:
2069 549 64
ApaI G_GGCC'C
Cuts at: 0 889 2350 2682
Size: 889 1461 332
Fragments arranged by size:
1461 889 332
ApaLI G'TGCA_C
Cuts at: 0 1330 2437 2682
Size: 1330 1107 245
Fragments arranged by size:
1330 1107 245
ApoI r'AATT_y
Cuts at: 0 840 2682
Size: 840 1842
AvaI C'yCGr_G
Cuts at: 0 573 754 805 906 1143 1492 1654 1720
Size: 573 181 51 101 237 349 162 66
Cuts at: 1720 1728 1996 2035 2266 2292 2343 2350 2682
Size: 8 268 39 231 26 51 7 332
Fragments arranged by size:
573 349 332 268 237 231 181 162
101 66 51 51 39 26 8 7
AvrII C'CTAG_G
Cuts at: 0 1809 2682
Size: 1809 873
BamHI G'GATC_C
Cuts at: 0 347 2682
Size: 347 2335
BanI G'GyrC_C
Cuts at: 0 256 1501 1716 1861 1981 2682
Size: 256 1245 215 145 120 701
Fragments arranged by size:
1245 701 256 215 145 120
BanII G_rGCy'C
Cuts at: 0 50 317 755 889 1010 1475 1499 1838
Size: 50 267 438 134 121 465 24 339
Cuts at: 1838 1991 2003 2042 2350 2682
Size: 153 12 39 308 332
Fragments arranged by size:
465 438 339 332 308 267 153 134
121 50 39 24 12
BbeI G_GCGC'C
Cuts at: 0 1865 2682
Size: 1865 817
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 1429 2536 2682
Size: 101 1328 1107 146
Fragments arranged by size:
1328 1107 146 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1308 1342 2183 2217 2682
Size: 1308 34 841 34 465
Fragments arranged by size:
1308 841 465 34 34
BclI T'GATC_A
Cuts at: 0 497 2682
Size: 497 2185
BglI GCCn_nnn'nGGC
Cuts at: 0 435 807 932 2294 2303 2456 2682
Size: 435 372 125 1362 9 153 226
Fragments arranged by size:
1362 435 372 226 153 125 9
BlpI GC'TnA_GC
Cuts at: 0 1684 2682
Size: 1684 998
Bme1580I G_kGCm'C
Cuts at: 0 519 889 937 1334 1721 2350 2441 2682
Size: 519 370 48 397 387 629 91 241
Fragments arranged by size:
629 519 397 387 370 241 91 48
BmgBI CAC'GTC
Cuts at: 0 2216 2682
Size: 2216 466
BmrI ACTGGGnnnn_n'
Cuts at: 0 1019 2682
Size: 1019 1663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 769 1657 2101 2626 2682
Size: 127 642 888 444 525 56
Fragments arranged by size:
888 642 525 444 127 56
Bpu10I CC'TnA_GC
Cuts at: 0 316 1210 2682
Size: 316 894 1472
Fragments arranged by size:
1472 894 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1513 2682
Size: 1393 120 1169
Fragments arranged by size:
1393 1169 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 576 2682
Size: 576 2106
BsaAI yAC'GTr
Cuts at: 0 2390 2682
Size: 2390 292
BsaBI GATnn'nnATC
Cuts at: 0 346 2682
Size: 346 2336
BsaHI Gr'CG_yC
Cuts at: 0 1862 2149 2654 2682
Size: 1862 287 505 28
Fragments arranged by size:
1862 505 287 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 594 624 2682
Size: 594 30 2058
Fragments arranged by size:
2058 594 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 291 465 1012 1606 1609 1618 1621
Size: 130 161 174 547 594 3 9 3
Cuts at: 1621 1624 1627 1630 1633 1636 1639 2682
Size: 3 3 3 3 3 3 1043
Fragments arranged by size:
1043 594 547 174 161 130 9 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 443 1016 1189 1575 1782 2029 2359 2682
Size: 443 573 173 386 207 247 330 323
Fragments arranged by size:
573 443 386 330 323 247 207 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 2682
Size: 453 2229
BsiEI CG_ry'CG
Cuts at: 0 1038 1458 1481 1538 2682
Size: 1038 420 23 57 1144
Fragments arranged by size:
1144 1038 420 57 23
BsiHKAI G_wGCw'C
Cuts at: 0 50 227 317 1216 1334 1393 1838 2228
Size: 50 177 90 899 118 59 445 390
Cuts at: 2228 2441 2639 2682
Size: 213 198 43
Fragments arranged by size:
899 445 390 213 198 177 118 90
59 50 43
Bsp1286I G_dGCh'C
Cuts at: 0 50 227 317 519 755 889 937 1010
Size: 50 177 90 202 236 134 48 73
Cuts at: 1010 1216 1334 1393 1475 1499 1721 1838 1991
Size: 206 118 59 82 24 222 117 153
Cuts at: 1991 2003 2042 2228 2350 2441 2639 2682
Size: 12 39 186 122 91 198 43
Fragments arranged by size:
236 222 206 202 198 186 177 153
134 122 118 117 91 90 82 73
59 50 48 43 39 24 12
BspHI T'CATG_A
Cuts at: 0 836 2162 2682
Size: 836 1326 520
Fragments arranged by size:
1326 836 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 386 878 2282 2682
Size: 170 216 492 1404 400
Fragments arranged by size:
1404 492 400 216 170
BsrBI CCG'CTC
Cuts at: 0 804 834 1460 2463 2682
Size: 804 30 626 1003 219
Fragments arranged by size:
1003 804 626 219 30
BsrDI GCAATG_nn'
Cuts at: 0 419 2682
Size: 419 2263
BsrFI r'CCGG_y
Cuts at: 0 333 759 1166 1334 1441 1453 1649 2441
Size: 333 426 407 168 107 12 196 792
Cuts at: 2441 2455 2682
Size: 14 227
Fragments arranged by size:
792 426 407 333 227 196 168 107
14 12
BssHII G'CGCG_C
Cuts at: 0 560 562 2498 2682
Size: 560 2 1936 184
Fragments arranged by size:
1936 560 184 2
BssSI C'ACGA_G
Cuts at: 0 1558 1582 2682
Size: 1558 24 1100
Fragments arranged by size:
1558 1100 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 674 1283 2682
Size: 674 609 1399
Fragments arranged by size:
1399 674 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1512 1758 2682
Size: 1512 246 924
Fragments arranged by size:
1512 924 246
BstYI r'GATC_y
Cuts at: 0 347 2682
Size: 347 2335
BtgI C'CryG_G
Cuts at: 0 781 818 1058 1079 1149 2682
Size: 781 37 240 21 70 1533
Fragments arranged by size:
1533 781 240 70 37 21
BtsI GCAGTG_nn'
Cuts at: 0 244 1816 2134 2682
Size: 244 1572 318 548
Fragments arranged by size:
1572 548 318 244
EaeI y'GGCC_r
Cuts at: 0 174 761 1061 1451 1455 1478 1535 1748
Size: 174 587 300 390 4 23 57 213
Cuts at: 1748 1841 2309 2682
Size: 93 468 373
Fragments arranged by size:
587 468 390 373 300 213 174 93
57 23 4
EagI C'GGCC_G
Cuts at: 0 1455 1478 1535 2682
Size: 1455 23 57 1147
Fragments arranged by size:
1455 1147 57 23
EarI CTCTTCn'nnn_
Cuts at: 0 952 2008 2682
Size: 952 1056 674
Fragments arranged by size:
1056 952 674
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1540 2460 2682
Size: 1540 920 222
Fragments arranged by size:
1540 920 222
EcoICRI GAG'CTC
Cuts at: 0 48 315 1836 2682
Size: 48 267 1521 846
Fragments arranged by size:
1521 846 267 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 769 933 1650 1657 1891 2101 2571
Size: 127 642 164 717 7 234 210 470
Cuts at: 2571 2626 2682
Size: 55 56
Fragments arranged by size:
717 642 470 234 210 164 127 56
55 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 2682
Size: 280 2402
EcoO109I rG'GnC_Cy
Cuts at: 0 273 886 1524 1725 2076 2169 2271 2347
Size: 273 613 638 201 351 93 102 76
Cuts at: 2347 2682
Size: 335
Fragments arranged by size:
638 613 351 335 273 201 102 93
76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 702 2190 2682
Size: 702 1488 492
Fragments arranged by size:
1488 702 492
FseI GG_CCGG'CC
Cuts at: 0 763 1457 2682
Size: 763 694 1225
Fragments arranged by size:
1225 763 694
FspI TGC'GCA
Cuts at: 0 877 2682
Size: 877 1805
HaeII r_GCGC'y
Cuts at: 0 527 1416 1759 1777 1865 2051 2682
Size: 527 889 343 18 88 186 631
Fragments arranged by size:
889 631 527 343 186 88 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 1459 1491 2215 2247 2682
Size: 594 32 833 32 724 32 435
Fragments arranged by size:
833 724 594 435 32 32 32
HincII GTy'rAC
Cuts at: 0 1953 2682
Size: 1953 729
KasI G'GCGC_C
Cuts at: 0 1861 2682
Size: 1861 821
MluI A'CGCG_T
Cuts at: 0 1385 2492 2682
Size: 1385 1107 190
Fragments arranged by size:
1385 1107 190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 2682
Size: 1487 1195
MscI TGG'CCA
Cuts at: 0 176 1063 2682
Size: 176 887 1619
Fragments arranged by size:
1619 887 176
MslI CAynn'nnrTG
Cuts at: 0 1025 2065 2682
Size: 1025 1040 617
Fragments arranged by size:
1040 1025 617
MspA1I CmG'CkG
Cuts at: 0 447 454 783 1151 1224 1374 2025 2303
Size: 447 7 329 368 73 150 651 278
Cuts at: 2303 2315 2564 2682
Size: 12 249 118
Fragments arranged by size:
651 447 368 329 278 249 150 118
73 12 7
NaeI GCC'GGC
Cuts at: 0 761 1443 1455 1651 2682
Size: 761 682 12 196 1031
Fragments arranged by size:
1031 761 682 196 12
NarI GG'CG_CC
Cuts at: 0 1862 2682
Size: 1862 820
NcoI C'CATG_G
Cuts at: 0 1058 1079 2682
Size: 1058 21 1603
Fragments arranged by size:
1603 1058 21
NgoMIV G'CCGG_C
Cuts at: 0 759 1441 1453 1649 2682
Size: 759 682 12 196 1033
Fragments arranged by size:
1033 759 682 196 12
NspI r_CATG'y
Cuts at: 0 677 1434 2682
Size: 677 757 1248
Fragments arranged by size:
1248 757 677
PasI CC'CwG_GG
Cuts at: 0 519 2682
Size: 519 2163
PflMI CCAn_nnn'nTGG
Cuts at: 0 972 2682
Size: 972 1710
PmlI CAC'GTG
Cuts at: 0 2390 2682
Size: 2390 292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2107 2139 2682
Size: 2107 32 543
Fragments arranged by size:
2107 543 32
PpuMI rG'GwC_Cy
Cuts at: 0 1725 2076 2169 2271 2682
Size: 1725 351 93 102 411
Fragments arranged by size:
1725 411 351 102 93
PshAI GACnn'nnGTC
Cuts at: 0 101 2682
Size: 101 2581
PspOMI G'GGCC_C
Cuts at: 0 885 2346 2682
Size: 885 1461 336
Fragments arranged by size:
1461 885 336
PstI C_TGCA'G
Cuts at: 0 559 713 2425 2682
Size: 559 154 1712 257
Fragments arranged by size:
1712 559 257 154
PvuII CAG'CTG
Cuts at: 0 447 1374 2682
Size: 447 927 1308
Fragments arranged by size:
1308 927 447
SacI G_AGCT'C
Cuts at: 0 50 317 1838 2682
Size: 50 267 1521 844
Fragments arranged by size:
1521 844 267 50
SacII CC_GC'GG
Cuts at: 0 784 1152 2682
Size: 784 368 1530
Fragments arranged by size:
1530 784 368
SanDI GG'GwC_CC
Cuts at: 0 1725 2682
Size: 1725 957
SapI GCTCTTCn'nnn_
Cuts at: 0 2008 2682
Size: 2008 674
ScaI AGT'ACT
Cuts at: 0 2398 2682
Size: 2398 284
SfcI C'TryA_G
Cuts at: 0 555 709 2421 2595 2682
Size: 555 154 1712 174 87
Fragments arranged by size:
1712 555 174 154 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 435 2294 2682
Size: 435 1859 388
Fragments arranged by size:
1859 435 388
SfoI GGC'GCC
Cuts at: 0 1863 2682
Size: 1863 819
SgrAI Cr'CCGG_yG
Cuts at: 0 1334 2441 2455 2682
Size: 1334 1107 14 227
Fragments arranged by size:
1334 1107 227 14
SmaI CCC'GGG
Cuts at: 0 756 908 1145 1722 1730 1998 2037 2268
Size: 756 152 237 577 8 268 39 231
Cuts at: 2268 2345 2352 2682
Size: 77 7 330
Fragments arranged by size:
756 577 330 268 237 231 152 77
39 8 7
SmlI C'TyrA_G
Cuts at: 0 1408 1528 2682
Size: 1408 120 1154
Fragments arranged by size:
1408 1154 120
SrfI GCCC'GGGC
Cuts at: 0 756 2345 2682
Size: 756 1589 337
Fragments arranged by size:
1589 756 337
StuI AGG'CCT
Cuts at: 0 438 2682
Size: 438 2244
StyI C'CwwG_G
Cuts at: 0 197 1058 1079 1751 1809 2366 2682
Size: 197 861 21 672 58 557 316
Fragments arranged by size:
861 672 557 316 197 58 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 244 402 1052 2682
Size: 133 111 158 650 1630
Fragments arranged by size:
1630 650 158 133 111
TatI w'GTAC_w
Cuts at: 0 2120 2396 2682
Size: 2120 276 286
Fragments arranged by size:
2120 286 276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 225 257 576 608 2682
Size: 225 32 319 32 2074
Fragments arranged by size:
2074 319 225 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1039 2682
Size: 1039 1643
XmaI C'CCGG_G
Cuts at: 0 754 906 1143 1720 1728 1996 2035 2266
Size: 754 152 237 577 8 268 39 231
Cuts at: 2266 2343 2350 2682
Size: 77 7 332
Fragments arranged by size:
754 577 332 268 237 231 152 77
39 8 7
XmnI GAAnn'nnTTC
Cuts at: 0 1180 2682
Size: 1180 1502
ZraI GAC'GTC
Cuts at: 0 2655 2682
Size: 2655 27
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KasI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI AscI
AseI AsiSI BaeI BbvCI BciVI BfrBI BglII BmtI
BplI BsaWI BsiWI BsmI BsmBI BspEI BsrGI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PsiI PspXI PsrI PvuI RsrII SalI SbfI
SexAI SgfI SnaBI SpeI SphI SspI SwaI XbaI
XcmI XhoI