Restriction map for
pFN21AE2513
(Linear) (Six-base) MAPSORT of: KIEE2513.seq Check: 2006 from: 1 to: 774
KIEE2513.orf
With 182 enzymes: SgfI *
February 25, 2014 15:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 774
Size: 442 332
AleI CACnn'nnGTG
Cuts at: 0 572 774
Size: 572 202
AvaI C'yCGr_G
Cuts at: 0 512 774
Size: 512 262
BanII G_rGCy'C
Cuts at: 0 212 608 774
Size: 212 396 166
Fragments arranged by size:
396 212 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 434 584 774
Size: 86 348 150 190
Fragments arranged by size:
348 190 150 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 774
Size: 307 34 433
Fragments arranged by size:
433 307 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 649 774
Size: 196 453 125
Fragments arranged by size:
453 196 125
Bpu10I CC'TnA_GC
Cuts at: 0 691 774
Size: 691 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 111 607 774
Size: 111 496 167
Fragments arranged by size:
496 167 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 774
Size: 19 30 725
Fragments arranged by size:
725 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 106 121 268 271 274 400 403
Size: 43 63 15 147 3 3 126 3
Cuts at: 403 417 774
Size: 14 357
Fragments arranged by size:
357 147 126 63 43 15 14 3
3 3
BseYI C'CCAG_C
Cuts at: 0 733 741 774
Size: 733 8 33
Fragments arranged by size:
733 33 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 350 604 774
Size: 320 30 254 170
Fragments arranged by size:
320 254 170 30
BsiHKAI G_wGCw'C
Cuts at: 0 212 608 774
Size: 212 396 166
Fragments arranged by size:
396 212 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 537 774
Size: 537 237
Bsp1286I G_dGCh'C
Cuts at: 0 212 608 774
Size: 212 396 166
Fragments arranged by size:
396 212 166
BsrBI CCG'CTC
Cuts at: 0 310 555 774
Size: 310 245 219
Fragments arranged by size:
310 245 219
BstYI r'GATC_y
Cuts at: 0 140 774
Size: 140 634
BtgI C'CryG_G
Cuts at: 0 446 774
Size: 446 328
BtsI GCAGTG_nn'
Cuts at: 0 128 774
Size: 128 646
ClaI AT'CG_AT
Cuts at: 0 563 774
Size: 563 211
EaeI y'GGCC_r
Cuts at: 0 473 774
Size: 473 301
EarI CTCTTCn'nnn_
Cuts at: 0 41 59 98 484 774
Size: 41 18 39 386 290
Fragments arranged by size:
386 290 41 39 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 379 774
Size: 379 395
EcoICRI GAG'CTC
Cuts at: 0 210 606 774
Size: 210 396 168
Fragments arranged by size:
396 210 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 442 649 774
Size: 196 246 207 125
Fragments arranged by size:
246 207 196 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 475 774
Size: 475 299
EcoO109I rG'GnC_Cy
Cuts at: 0 216 737 774
Size: 216 521 37
Fragments arranged by size:
521 216 37
HaeII r_GCGC'y
Cuts at: 0 297 351 768 774
Size: 297 54 417 6
Fragments arranged by size:
417 297 54 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 774
Size: 110 32 632
Fragments arranged by size:
632 110 32
MscI TGG'CCA
Cuts at: 0 475 774
Size: 475 299
MslI CAynn'nnrTG
Cuts at: 0 572 774
Size: 572 202
MspA1I CmG'CkG
Cuts at: 0 627 774
Size: 627 147
NcoI C'CATG_G
Cuts at: 0 446 774
Size: 446 328
PpuMI rG'GwC_Cy
Cuts at: 0 737 774
Size: 737 37
PvuII CAG'CTG
Cuts at: 0 627 774
Size: 627 147
SacI G_AGCT'C
Cuts at: 0 212 608 774
Size: 212 396 166
Fragments arranged by size:
396 212 166
SanDI GG'GwC_CC
Cuts at: 0 737 774
Size: 737 37
SfcI C'TryA_G
Cuts at: 0 459 774
Size: 459 315
SmaI CCC'GGG
Cuts at: 0 514 774
Size: 514 260
StyI C'CwwG_G
Cuts at: 0 446 476 570 599 746 774
Size: 446 30 94 29 147 28
Fragments arranged by size:
446 147 94 30 29 28
XmaI C'CCGG_G
Cuts at: 0 512 774
Size: 512 262
Enzymes that do cut:
AcuI AleI AvaI BanII BbsI BcgI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BsrBI BstYI BtgI BtsI ClaI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI
MspA1I NcoI PpuMI PvuII SacI SanDI SfcI SmaI
StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI