Restriction map for
pFN21AE2507
(Linear) (Six-base) MAPSORT of: KIEE2507.seq Check: 565 from: 1 to: 1320
KIEE2507.orf
With 182 enzymes: SgfI *
February 14, 2014 11:03 ..
Acc65I G'GTAC_C
Cuts at: 0 1139 1320
Size: 1139 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 856 873 931 1060 1320
Size: 154 702 17 58 129 260
Fragments arranged by size:
702 260 154 129 58 17
AhdI GACnn_n'nnGTC
Cuts at: 0 168 321 1320
Size: 168 153 999
Fragments arranged by size:
999 168 153
AleI CACnn'nnGTG
Cuts at: 0 1307 1320
Size: 1307 13
AlwNI CAG_nnn'CTG
Cuts at: 0 447 675 692 834 1186 1320
Size: 447 228 17 142 352 134
Fragments arranged by size:
447 352 228 142 134 17
ApaI G_GGCC'C
Cuts at: 0 68 219 907 1320
Size: 68 151 688 413
Fragments arranged by size:
688 413 151 68
ApaLI G'TGCA_C
Cuts at: 0 871 1108 1320
Size: 871 237 212
Fragments arranged by size:
871 237 212
AvaI C'yCGr_G
Cuts at: 0 794 1285 1320
Size: 794 491 35
Fragments arranged by size:
794 491 35
BamHI G'GATC_C
Cuts at: 0 512 848 1025 1320
Size: 512 336 177 295
Fragments arranged by size:
512 336 295 177
BanI G'GyrC_C
Cuts at: 0 1139 1320
Size: 1139 181
BanII G_rGCy'C
Cuts at: 0 68 219 907 1121 1320
Size: 68 151 688 214 199
Fragments arranged by size:
688 214 199 151 68
BglI GCCn_nnn'nGGC
Cuts at: 0 151 1320
Size: 151 1169
Bme1580I G_kGCm'C
Cuts at: 0 68 219 362 460 875 907 1112 1320
Size: 68 151 143 98 415 32 205 208
Fragments arranged by size:
415 208 205 151 143 98 68 32
BmgBI CAC'GTC
Cuts at: 0 141 1320
Size: 141 1179
BmrI ACTGGGnnnn_n'
Cuts at: 0 1092 1320
Size: 1092 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1320
Size: 277 1043
Bpu10I CC'TnA_GC
Cuts at: 0 1115 1320
Size: 1115 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1292 1320
Size: 517 775 28
Fragments arranged by size:
775 517 28
BsaBI GATnn'nnATC
Cuts at: 0 127 1320
Size: 127 1193
BsaHI Gr'CG_yC
Cuts at: 0 41 367 496 1320
Size: 41 326 129 824
Fragments arranged by size:
824 326 129 41
BsaWI w'CCGG_w
Cuts at: 0 287 1320
Size: 287 1033
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1000 1320
Size: 391 609 320
Fragments arranged by size:
609 391 320
BseYI C'CCAG_C
Cuts at: 0 642 1179 1204 1320
Size: 642 537 25 116
Fragments arranged by size:
642 537 116 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 96 421 688 918 1320
Size: 55 41 325 267 230 402
Fragments arranged by size:
402 325 267 230 55 41
BsiEI CG_ry'CG
Cuts at: 0 329 386 1320
Size: 329 57 934
Fragments arranged by size:
934 329 57
BsiHKAI G_wGCw'C
Cuts at: 0 602 725 875 1112 1121 1320
Size: 602 123 150 237 9 199
Fragments arranged by size:
602 237 199 150 123 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 373 1320
Size: 373 947
Bsp1286I G_dGCh'C
Cuts at: 0 68 219 362 460 602 725 875 907
Size: 68 151 143 98 142 123 150 32
Cuts at: 907 1112 1121 1320
Size: 205 9 199
Fragments arranged by size:
205 199 151 150 143 142 123 98
68 32 9
BspEI T'CCGG_A
Cuts at: 0 287 1320
Size: 287 1033
BspHI T'CATG_A
Cuts at: 0 56 1320
Size: 56 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 1320
Size: 683 637
BsrBI CCG'CTC
Cuts at: 0 636 1320
Size: 636 684
BsrDI GCAATG_nn'
Cuts at: 0 91 341 1320
Size: 91 250 979
Fragments arranged by size:
979 250 91
BsrFI r'CCGG_y
Cuts at: 0 419 1320
Size: 419 901
BssSI C'ACGA_G
Cuts at: 0 461 874 1320
Size: 461 413 446
Fragments arranged by size:
461 446 413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 1320
Size: 675 645
BstYI r'GATC_y
Cuts at: 0 182 482 512 848 1001 1025 1320
Size: 182 300 30 336 153 24 295
Fragments arranged by size:
336 300 295 182 153 30 24
BtgI C'CryG_G
Cuts at: 0 76 362 516 852 1224 1320
Size: 76 286 154 336 372 96
Fragments arranged by size:
372 336 286 154 96 76
BtsI GCAGTG_nn'
Cuts at: 0 303 569 705 1320
Size: 303 266 136 615
Fragments arranged by size:
615 303 266 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 1102 1320
Size: 751 351 218
Fragments arranged by size:
751 351 218
EaeI y'GGCC_r
Cuts at: 0 102 143 326 383 1269 1320
Size: 102 41 183 57 886 51
Fragments arranged by size:
886 183 102 57 51 41
EagI C'GGCC_G
Cuts at: 0 326 383 1320
Size: 326 57 937
Fragments arranged by size:
937 326 57
EarI CTCTTCn'nnn_
Cuts at: 0 607 685 1320
Size: 607 78 635
Fragments arranged by size:
635 607 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1306 1320
Size: 1306 14
EcoICRI GAG'CTC
Cuts at: 0 1119 1320
Size: 1119 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 277 856 873 931 1060 1320
Size: 154 123 579 17 58 129 260
Fragments arranged by size:
579 260 154 129 123 58 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 838 1320
Size: 202 636 482
Fragments arranged by size:
636 482 202
EcoO109I rG'GnC_Cy
Cuts at: 0 27 65 195 215 216 252 672 773
Size: 27 38 130 20 1 36 420 101
Cuts at: 773 831 903 904 1320
Size: 58 72 1 416
Fragments arranged by size:
420 416 130 101 72 58 38 36
27 20 1 1
HaeII r_GCGC'y
Cuts at: 0 448 506 571 643 823 1211 1320
Size: 448 58 65 72 180 388 109
Fragments arranged by size:
448 388 180 109 72 65 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 38 70 154 186 307 339 481 513
Size: 38 32 84 32 121 32 142 32
Cuts at: 513 952 984 1320
Size: 439 32 336
Fragments arranged by size:
439 336 142 121 84 38 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1053 1320
Size: 1053 267
KpnI G_GTAC'C
Cuts at: 0 1143 1320
Size: 1143 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 602 860 1320
Size: 69 533 258 460
Fragments arranged by size:
533 460 258 69
MscI TGG'CCA
Cuts at: 0 145 1271 1320
Size: 145 1126 49
Fragments arranged by size:
1126 145 49
MslI CAynn'nnrTG
Cuts at: 0 976 1307 1320
Size: 976 331 13
Fragments arranged by size:
976 331 13
MspA1I CmG'CkG
Cuts at: 0 78 364 518 854 1183 1320
Size: 78 286 154 336 329 137
Fragments arranged by size:
336 329 286 154 137 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 1082 1320
Size: 1082 238
PpuMI rG'GwC_Cy
Cuts at: 0 195 252 672 1320
Size: 195 57 420 648
Fragments arranged by size:
648 420 195 57
PspOMI G'GGCC_C
Cuts at: 0 64 215 903 1320
Size: 64 151 688 417
Fragments arranged by size:
688 417 151 64
PstI C_TGCA'G
Cuts at: 0 653 671 1320
Size: 653 18 649
Fragments arranged by size:
653 649 18
PvuII CAG'CTG
Cuts at: 0 1183 1320
Size: 1183 137
SacI G_AGCT'C
Cuts at: 0 1121 1320
Size: 1121 199
SacII CC_GC'GG
Cuts at: 0 79 365 519 855 1320
Size: 79 286 154 336 465
Fragments arranged by size:
465 336 286 154 79
SapI GCTCTTCn'nnn_
Cuts at: 0 607 685 1320
Size: 607 78 635
Fragments arranged by size:
635 607 78
SbfI CC_TGCA'GG
Cuts at: 0 671 1320
Size: 671 649
SfcI C'TryA_G
Cuts at: 0 649 667 1134 1320
Size: 649 18 467 186
Fragments arranged by size:
649 467 186 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 151 1320
Size: 151 1169
SmaI CCC'GGG
Cuts at: 0 796 1320
Size: 796 524
StuI AGG'CCT
Cuts at: 0 583 1320
Size: 583 737
StyI C'CwwG_G
Cuts at: 0 527 578 1320
Size: 527 51 742
Fragments arranged by size:
742 527 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 936 1093 1320
Size: 936 157 227
Fragments arranged by size:
936 227 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 986 1018 1320
Size: 282 32 672 32 302
Fragments arranged by size:
672 302 282 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 545 1320
Size: 545 775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 1320
Size: 252 1068
XmaI C'CCGG_G
Cuts at: 0 794 1320
Size: 794 526
Enzymes that do cut:
Acc65I AcuI AhdI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstYI BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
PflMI PpuMI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SfiI SmaI StuI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpuEI BsaAI BsaXI BsiWI BsmI
BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XhoI XmnI
ZraI