Restriction map for
pFN21AE2490
(Linear) (Six-base) MAPSORT of: KIEE2490.seq Check: 1152 from: 1 to: 1284
KIEE2490.orf
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
Acc65I G'GTAC_C
Cuts at: 0 474 1284
Size: 474 810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1284
Size: 438 846
AfeI AGC'GCT
Cuts at: 0 57 1284
Size: 57 1227
AleI CACnn'nnGTG
Cuts at: 0 199 1284
Size: 199 1085
AlwNI CAG_nnn'CTG
Cuts at: 0 1269 1284
Size: 1269 15
ApoI r'AATT_y
Cuts at: 0 182 540 1284
Size: 182 358 744
Fragments arranged by size:
744 358 182
AvrII C'CTAG_G
Cuts at: 0 597 661 1284
Size: 597 64 623
Fragments arranged by size:
623 597 64
BamHI G'GATC_C
Cuts at: 0 505 1284
Size: 505 779
BanI G'GyrC_C
Cuts at: 0 474 1284
Size: 474 810
BanII G_rGCy'C
Cuts at: 0 320 771 905 1284
Size: 320 451 134 379
Fragments arranged by size:
451 379 320 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 1284
Size: 98 1186
BbvCI CC'TCA_GC
Cuts at: 0 922 1284
Size: 922 362
BciVI GTATCCnnnnn_n'
Cuts at: 0 51 1284
Size: 51 1233
BglI GCCn_nnn'nGGC
Cuts at: 0 1243 1284
Size: 1243 41
BglII A'GATC_T
Cuts at: 0 449 1284
Size: 449 835
Bme1580I G_kGCm'C
Cuts at: 0 19 118 797 1284
Size: 19 99 679 487
Fragments arranged by size:
679 487 99 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 627 1284
Size: 364 263 657
Fragments arranged by size:
657 364 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1284
Size: 793 491
Bpu10I CC'TnA_GC
Cuts at: 0 290 922 1284
Size: 290 632 362
Fragments arranged by size:
632 362 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 298 1284
Size: 298 986
BsaWI w'CCGG_w
Cuts at: 0 803 1284
Size: 803 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 798 828 1284
Size: 798 30 456
Fragments arranged by size:
798 456 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 970 1284
Size: 970 314
BseYI C'CCAG_C
Cuts at: 0 1232 1284
Size: 1232 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1284
Size: 223 1061
BsiHKAI G_wGCw'C
Cuts at: 0 172 1284
Size: 172 1112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1219 1284
Size: 1219 65
Bsp1286I G_dGCh'C
Cuts at: 0 19 118 172 320 771 797 905 1284
Size: 19 99 54 148 451 26 108 379
Fragments arranged by size:
451 379 148 108 99 54 26 19
BspEI T'CCGG_A
Cuts at: 0 803 1284
Size: 803 481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 210 1284
Size: 210 1074
BstXI CCAn_nnnn'nTGG
Cuts at: 0 871 1284
Size: 871 413
BstYI r'GATC_y
Cuts at: 0 449 505 842 1134 1284
Size: 449 56 337 292 150
Fragments arranged by size:
449 337 292 150 56
Bsu36I CC'TnA_GG
Cuts at: 0 1110 1284
Size: 1110 174
BtsI GCAGTG_nn'
Cuts at: 0 492 1284
Size: 492 792
DraI TTT'AAA
Cuts at: 0 1049 1284
Size: 1049 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 1189 1284
Size: 1189 95
EaeI y'GGCC_r
Cuts at: 0 2 329 564 1284
Size: 2 327 235 720
Fragments arranged by size:
720 327 235 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 793 1284
Size: 438 355 491
Fragments arranged by size:
491 438 355
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1159 1284
Size: 1159 125
EcoO109I rG'GnC_Cy
Cuts at: 0 587 1284
Size: 587 697
EcoRI G'AATT_C
Cuts at: 0 182 1284
Size: 182 1102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1185 1284
Size: 468 717 99
Fragments arranged by size:
717 468 99
HaeII r_GCGC'y
Cuts at: 0 59 134 1284
Size: 59 75 1150
Fragments arranged by size:
1150 75 59
HincII GTy'rAC
Cuts at: 0 1011 1284
Size: 1011 273
KpnI G_GTAC'C
Cuts at: 0 478 1284
Size: 478 806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 809 1284
Size: 533 276 475
Fragments arranged by size:
533 475 276
MscI TGG'CCA
Cuts at: 0 4 331 566 1284
Size: 4 327 235 718
Fragments arranged by size:
718 327 235 4
MslI CAynn'nnrTG
Cuts at: 0 34 199 1018 1284
Size: 34 165 819 266
Fragments arranged by size:
819 266 165 34
MspA1I CmG'CkG
Cuts at: 0 558 1284
Size: 558 726
PasI CC'CwG_GG
Cuts at: 0 762 1284
Size: 762 522
PflMI CCAn_nnn'nTGG
Cuts at: 0 11 1284
Size: 11 1273
PpuMI rG'GwC_Cy
Cuts at: 0 587 1284
Size: 587 697
PsiI TTA'TAA
Cuts at: 0 704 1284
Size: 704 580
PvuII CAG'CTG
Cuts at: 0 558 1284
Size: 558 726
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1243 1284
Size: 1243 41
StyI C'CwwG_G
Cuts at: 0 422 590 597 661 1238 1284
Size: 422 168 7 64 577 46
Fragments arranged by size:
577 422 168 64 46 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 628 1016 1284
Size: 628 388 268
Fragments arranged by size:
628 388 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 1284
Size: 823 32 429
Fragments arranged by size:
823 429 32
XbaI T'CTAG_A
Cuts at: 0 231 1284
Size: 231 1053
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApoI AvrII BamHI
BanI BanII BbsI BbvCI BciVI BglI BglII Bme1580I
BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BstAPI BstXI BstYI
Bsu36I BtsI DraI DrdI EaeI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII HincII KpnI MmeI MscI MslI
MspA1I PasI PflMI PpuMI PsiI PvuII SfiI StyI
TaqII TstI XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BbeI BcgI BclI BfrBI BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraIII
EagI EarI EciI EcoICRI EcoRV FseI FspI FspAI
Hin4I HindIII HpaI KasI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XcmI XhoI XmaI XmnI
ZraI