Restriction map for
pFN21AE2483
(Linear) (Six-base) MAPSORT of: KIEE2483.seq Check: 6969 from: 1 to: 1005
KIEE2483.orf
With 182 enzymes: SgfI *
September 20, 2013 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 1005
Size: 169 836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 727 762 1005
Size: 484 243 35 243
Fragments arranged by size:
484 243 243 35
AflII C'TTAA_G
Cuts at: 0 172 1005
Size: 172 833
AleI CACnn'nnGTG
Cuts at: 0 101 1005
Size: 101 904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 997 1005
Size: 997 8
AlwNI CAG_nnn'CTG
Cuts at: 0 864 1005
Size: 864 141
ApoI r'AATT_y
Cuts at: 0 502 1005
Size: 502 503
BamHI G'GATC_C
Cuts at: 0 380 1005
Size: 380 625
BanI G'GyrC_C
Cuts at: 0 433 1005
Size: 433 572
BbsI GAAGACnn'nnnn_
Cuts at: 0 138 1005
Size: 138 867
BglI GCCn_nnn'nGGC
Cuts at: 0 176 1005
Size: 176 829
BglII A'GATC_T
Cuts at: 0 869 1005
Size: 869 136
BmrI ACTGGGnnnn_n'
Cuts at: 0 229 676 894 1005
Size: 229 447 218 111
Fragments arranged by size:
447 229 218 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 729 1005
Size: 729 276
BsaI GGTCTCn'nnnn_
Cuts at: 0 583 1005
Size: 583 422
BsaAI yAC'GTr
Cuts at: 0 167 612 1005
Size: 167 445 393
Fragments arranged by size:
445 393 167
BsaHI Gr'CG_yC
Cuts at: 0 922 1005
Size: 922 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 834 864 880 910 1005
Size: 592 30 212 30 16 30 95
Fragments arranged by size:
592 212 95 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 130 152 1005
Size: 49 81 22 853
Fragments arranged by size:
853 81 49 22
BseYI C'CCAG_C
Cuts at: 0 52 355 996 1005
Size: 52 303 641 9
Fragments arranged by size:
641 303 52 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 630 1005
Size: 147 483 375
Fragments arranged by size:
483 375 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 193 913 1005
Size: 193 720 92
Fragments arranged by size:
720 193 92
BspHI T'CATG_A
Cuts at: 0 453 1005
Size: 453 552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 401 1005
Size: 169 232 604
Fragments arranged by size:
604 232 169
BsrDI GCAATG_nn'
Cuts at: 0 239 369 1005
Size: 239 130 636
Fragments arranged by size:
636 239 130
BssHII G'CGCG_C
Cuts at: 0 35 1005
Size: 35 970
BssSI C'ACGA_G
Cuts at: 0 627 1005
Size: 627 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 103 444 699 1005
Size: 103 341 255 306
Fragments arranged by size:
341 306 255 103
BstYI r'GATC_y
Cuts at: 0 380 869 1005
Size: 380 489 136
Fragments arranged by size:
489 380 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 960 1005
Size: 960 45
EaeI y'GGCC_r
Cuts at: 0 20 446 1005
Size: 20 426 559
Fragments arranged by size:
559 426 20
EarI CTCTTCn'nnn_
Cuts at: 0 781 1005
Size: 781 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 727 729 762 1005
Size: 484 243 2 33 243
Fragments arranged by size:
484 243 243 33 2
EcoO109I rG'GnC_Cy
Cuts at: 0 303 1005
Size: 303 702
EcoRI G'AATT_C
Cuts at: 0 502 1005
Size: 502 503
EcoRV GAT'ATC
Cuts at: 0 75 1005
Size: 75 930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 1005
Size: 927 78
FspI TGC'GCA
Cuts at: 0 946 1005
Size: 946 59
FspAI rTGC'GCAy
Cuts at: 0 946 1005
Size: 946 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 880 912 1005
Size: 128 32 720 32 93
Fragments arranged by size:
720 128 93 32 32
HincII GTy'rAC
Cuts at: 0 579 1005
Size: 579 426
MscI TGG'CCA
Cuts at: 0 448 1005
Size: 448 557
MslI CAynn'nnrTG
Cuts at: 0 101 1005
Size: 101 904
MspA1I CmG'CkG
Cuts at: 0 782 1005
Size: 782 223
NspI r_CATG'y
Cuts at: 0 577 1005
Size: 577 428
PasI CC'CwG_GG
Cuts at: 0 699 1005
Size: 699 306
PfoI T'CCnGG_A
Cuts at: 0 305 1005
Size: 305 700
PmlI CAC'GTG
Cuts at: 0 167 1005
Size: 167 838
PpuMI rG'GwC_Cy
Cuts at: 0 303 1005
Size: 303 702
PstI C_TGCA'G
Cuts at: 0 869 1005
Size: 869 136
PvuII CAG'CTG
Cuts at: 0 782 1005
Size: 782 223
SanDI GG'GwC_CC
Cuts at: 0 303 1005
Size: 303 702
SfcI C'TryA_G
Cuts at: 0 483 865 956 1005
Size: 483 382 91 49
Fragments arranged by size:
483 382 91 49
SmlI C'TyrA_G
Cuts at: 0 172 1005
Size: 172 833
StuI AGG'CCT
Cuts at: 0 469 1005
Size: 469 536
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 1005
Size: 369 636
XmnI GAAnn'nnTTC
Cuts at: 0 469 1005
Size: 469 536
Enzymes that do cut:
AarI AcuI AflII AleI AlfI AlwNI ApoI BamHI
BanI BbsI BglI BglII BmrI BpmI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsmBI BspHI BspMI
BsrDI BssHII BssSI BstXI BstYI BtgZI EaeI EarI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI Hin4I
HincII MscI MslI MspA1I NspI PasI PfoI PmlI
PpuMI PstI PvuII SanDI SfcI SmlI StuI TaqII
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BsrBI
BsrFI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI FseI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI