Restriction map for
pFN21AE2481
(Linear) (Six-base) MAPSORT of: KIEE2481.seq Check: 8422 from: 1 to: 1416
KIEE2481.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AatII G_ACGT'C
Cuts at: 0 850 1416
Size: 850 566
AccI GT'mk_AC
Cuts at: 0 46 1416
Size: 46 1370
AclI AA'CG_TT
Cuts at: 0 1068 1416
Size: 1068 348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 997 1416
Size: 997 419
AlwNI CAG_nnn'CTG
Cuts at: 0 12 1416
Size: 12 1404
ApaI G_GGCC'C
Cuts at: 0 692 1416
Size: 692 724
ApaLI G'TGCA_C
Cuts at: 0 991 1416
Size: 991 425
ApoI r'AATT_y
Cuts at: 0 124 787 1416
Size: 124 663 629
Fragments arranged by size:
663 629 124
AvaI C'yCGr_G
Cuts at: 0 119 176 452 598 1052 1416
Size: 119 57 276 146 454 364
Fragments arranged by size:
454 364 276 146 119 57
BamHI G'GATC_C
Cuts at: 0 463 1396 1416
Size: 463 933 20
Fragments arranged by size:
933 463 20
BanI G'GyrC_C
Cuts at: 0 169 949 1195 1416
Size: 169 780 246 221
Fragments arranged by size:
780 246 221 169
BanII G_rGCy'C
Cuts at: 0 223 631 692 1368 1416
Size: 223 408 61 676 48
Fragments arranged by size:
676 408 223 61 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 419 512 852 1145 1416
Size: 12 407 93 340 293 271
Fragments arranged by size:
407 340 293 271 93 12
BbvCI CC'TCA_GC
Cuts at: 0 302 1416
Size: 302 1114
BglI GCCn_nnn'nGGC
Cuts at: 0 266 1416
Size: 266 1150
BlpI GC'TnA_GC
Cuts at: 0 584 1416
Size: 584 832
Bme1580I G_kGCm'C
Cuts at: 0 172 692 995 1416
Size: 172 520 303 421
Fragments arranged by size:
520 421 303 172
BmgBI CAC'GTC
Cuts at: 0 155 1416
Size: 155 1261
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 1416
Size: 174 1242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1416
Size: 757 659
Bpu10I CC'TnA_GC
Cuts at: 0 302 343 1416
Size: 302 41 1073
Fragments arranged by size:
1073 302 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1416
Size: 1141 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 142 284 882 1104 1416
Size: 142 142 598 222 312
Fragments arranged by size:
598 312 222 142 142
BsaHI Gr'CG_yC
Cuts at: 0 847 1416
Size: 847 569
BsaWI w'CCGG_w
Cuts at: 0 1360 1393 1416
Size: 1360 33 23
Fragments arranged by size:
1360 33 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 375 405 1416
Size: 139 30 206 30 1011
Fragments arranged by size:
1011 206 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 148 157 160 874 1159 1416
Size: 45 103 9 3 714 285 257
Fragments arranged by size:
714 285 257 103 45 9 3
BseYI C'CCAG_C
Cuts at: 0 307 337 443 550 614 1416
Size: 307 30 106 107 64 802
Fragments arranged by size:
802 307 107 106 64 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 880 1416
Size: 94 786 536
Fragments arranged by size:
786 536 94
BsiEI CG_ry'CG
Cuts at: 0 670 1416
Size: 670 746
BsiHKAI G_wGCw'C
Cuts at: 0 995 1416
Size: 995 421
Bsp1286I G_dGCh'C
Cuts at: 0 172 223 631 692 995 1368 1416
Size: 172 51 408 61 303 373 48
Fragments arranged by size:
408 373 303 172 61 51 48
BspEI T'CCGG_A
Cuts at: 0 1393 1416
Size: 1393 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 830 1416
Size: 830 586
BsrBI CCG'CTC
Cuts at: 0 272 1416
Size: 272 1144
BsrDI GCAATG_nn'
Cuts at: 0 700 1416
Size: 700 716
BsrFI r'CCGG_y
Cuts at: 0 15 646 1265 1416
Size: 15 631 619 151
Fragments arranged by size:
631 619 151 15
BssSI C'ACGA_G
Cuts at: 0 287 840 1144 1416
Size: 287 553 304 272
Fragments arranged by size:
553 304 287 272
BstAPI GCAn_nnn'nTGC
Cuts at: 0 12 990 1416
Size: 12 978 426
Fragments arranged by size:
978 426 12
BstBI TT'CG_AA
Cuts at: 0 679 1416
Size: 679 737
BstEII G'GTnAC_C
Cuts at: 0 1305 1416
Size: 1305 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 534 1416
Size: 501 33 882
Fragments arranged by size:
882 501 33
BstYI r'GATC_y
Cuts at: 0 463 1337 1396 1416
Size: 463 874 59 20
Fragments arranged by size:
874 463 59 20
Bsu36I CC'TnA_GG
Cuts at: 0 418 1416
Size: 418 998
BtgI C'CryG_G
Cuts at: 0 914 1416
Size: 914 502
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 183 578 1416
Size: 183 395 838
Fragments arranged by size:
838 395 183
BtsI GCAGTG_nn'
Cuts at: 0 27 730 1416
Size: 27 703 686
Fragments arranged by size:
703 686 27
EaeI y'GGCC_r
Cuts at: 0 917 1106 1416
Size: 917 189 310
Fragments arranged by size:
917 310 189
EarI CTCTTCn'nnn_
Cuts at: 0 483 689 704 1177 1223 1416
Size: 483 206 15 473 46 193
Fragments arranged by size:
483 473 206 193 46 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 832 1416
Size: 832 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1416
Size: 757 659
EcoO109I rG'GnC_Cy
Cuts at: 0 9 1416
Size: 9 1407
EcoRI G'AATT_C
Cuts at: 0 124 787 1416
Size: 124 663 629
Fragments arranged by size:
663 629 124
HaeII r_GCGC'y
Cuts at: 0 405 1416
Size: 405 1011
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 667 699 749 781 1334 1366
Size: 139 32 496 32 50 32 553 32
Cuts at: 1366 1416
Size: 50
Fragments arranged by size:
553 496 139 50 50 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 600 1416
Size: 410 190 816
Fragments arranged by size:
816 410 190
MspA1I CmG'CkG
Cuts at: 0 501 1116 1416
Size: 501 615 300
Fragments arranged by size:
615 501 300
NruI TCG'CGA
Cuts at: 0 672 1416
Size: 672 744
PasI CC'CwG_GG
Cuts at: 0 264 1354 1416
Size: 264 1090 62
Fragments arranged by size:
1090 264 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1296 1416
Size: 1296 120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1416
Size: 76 32 1308
Fragments arranged by size:
1308 76 32
PpuMI rG'GwC_Cy
Cuts at: 0 9 1416
Size: 9 1407
PspOMI G'GGCC_C
Cuts at: 0 688 1416
Size: 688 728
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 551 583 1416
Size: 551 32 833
Fragments arranged by size:
833 551 32
PstI C_TGCA'G
Cuts at: 0 986 1380 1416
Size: 986 394 36
Fragments arranged by size:
986 394 36
PvuI CG_AT'CG
Cuts at: 0 670 1416
Size: 670 746
PvuII CAG'CTG
Cuts at: 0 1116 1416
Size: 1116 300
SapI GCTCTTCn'nnn_
Cuts at: 0 689 1416
Size: 689 727
SfcI C'TryA_G
Cuts at: 0 47 982 1376 1416
Size: 47 935 394 40
Fragments arranged by size:
935 394 47 40
SmaI CCC'GGG
Cuts at: 0 121 178 454 1416
Size: 121 57 276 962
Fragments arranged by size:
962 276 121 57
SmlI C'TyrA_G
Cuts at: 0 1156 1416
Size: 1156 260
StuI AGG'CCT
Cuts at: 0 869 1024 1416
Size: 869 155 392
Fragments arranged by size:
869 392 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 1183 1416
Size: 435 748 233
Fragments arranged by size:
748 435 233
TatI w'GTAC_w
Cuts at: 0 827 1416
Size: 827 589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 1416
Size: 375 32 1009
Fragments arranged by size:
1009 375 32
Tth111I GACn'n_nGTC
Cuts at: 0 153 1416
Size: 153 1263
XmaI C'CCGG_G
Cuts at: 0 119 176 452 1416
Size: 119 57 276 964
Fragments arranged by size:
964 276 119 57
ZraI GAC'GTC
Cuts at: 0 848 1416
Size: 848 568
Enzymes that do cut:
AatII AccI AclI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI
MspA1I NruI PasI PflMI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII SapI SfcI SmaI SmlI StuI
TaqII TatI TstI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AfeI AflII AflIII AgeI AhdI
AleI AloI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BglII BmtI BplI BsaAI
BsaBI BsiWI BsmI BsmBI BspHI BsrGI BssHII BstZ17I
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PciI PfoI PmeI PmlI PshAI PsiI PspXI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI XbaI XcmI XhoI XmnI