Restriction map for
pFN21AE2476
(Linear) (Six-base) MAPSORT of: KIEE2476.seq Check: 705 from: 1 to: 1500
KIEE2476.orf
With 182 enzymes: SgfI *
June 18, 2013 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 591 805 1500
Size: 591 214 695
Fragments arranged by size:
695 591 214
AccI GT'mk_AC
Cuts at: 0 977 1500
Size: 977 523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 949 1078 1480 1500
Size: 561 388 129 402 20
Fragments arranged by size:
561 402 388 129 20
AflIII A'CryG_T
Cuts at: 0 258 1500
Size: 258 1242
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 728 760 1500
Size: 728 32 740
Fragments arranged by size:
740 728 32
ApoI r'AATT_y
Cuts at: 0 787 1149 1500
Size: 787 362 351
Fragments arranged by size:
787 362 351
AvaI C'yCGr_G
Cuts at: 0 37 1206 1287 1500
Size: 37 1169 81 213
Fragments arranged by size:
1169 213 81 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 653 686 1500
Size: 653 33 814
Fragments arranged by size:
814 653 33
BanII G_rGCy'C
Cuts at: 0 1213 1500
Size: 1213 287
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 404 1066 1396 1406 1500
Size: 317 87 662 330 10 94
Fragments arranged by size:
662 330 317 94 87 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1268 1302 1500
Size: 1268 34 198
Fragments arranged by size:
1268 198 34
BclI T'GATC_A
Cuts at: 0 891 1500
Size: 891 609
BglI GCCn_nnn'nGGC
Cuts at: 0 97 1500
Size: 97 1403
Bme1580I G_kGCm'C
Cuts at: 0 28 1500
Size: 28 1472
BsaWI w'CCGG_w
Cuts at: 0 1180 1500
Size: 1180 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 728 758 1186 1216 1500
Size: 728 30 428 30 284
Fragments arranged by size:
728 428 284 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 1409 1500
Size: 568 841 91
Fragments arranged by size:
841 568 91
BseYI C'CCAG_C
Cuts at: 0 763 1500
Size: 763 737
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 619 1500
Size: 34 585 881
Fragments arranged by size:
881 585 34
BsiHKAI G_wGCw'C
Cuts at: 0 38 288 1500
Size: 38 250 1212
Fragments arranged by size:
1212 250 38
BsmI GAATG_Cn'
Cuts at: 0 1412 1500
Size: 1412 88
Bsp1286I G_dGCh'C
Cuts at: 0 28 38 288 1213 1500
Size: 28 10 250 925 287
Fragments arranged by size:
925 287 250 28 10
BspHI T'CATG_A
Cuts at: 0 783 1500
Size: 783 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 591 805 1500
Size: 591 214 695
Fragments arranged by size:
695 591 214
BsrDI GCAATG_nn'
Cuts at: 0 566 775 1500
Size: 566 209 725
Fragments arranged by size:
725 566 209
BsrFI r'CCGG_y
Cuts at: 0 100 1500
Size: 100 1400
BstBI TT'CG_AA
Cuts at: 0 198 1500
Size: 198 1302
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 1500
Size: 504 996
BstYI r'GATC_y
Cuts at: 0 321 1500
Size: 321 1179
Bsu36I CC'TnA_GG
Cuts at: 0 419 1500
Size: 419 1081
BtgI C'CryG_G
Cuts at: 0 626 690 1432 1500
Size: 626 64 742 68
Fragments arranged by size:
742 626 68 64
DraIII CAC_nnn'GTG
Cuts at: 0 720 1500
Size: 720 780
EarI CTCTTCn'nnn_
Cuts at: 0 568 1500
Size: 568 932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 949 1078 1480 1500
Size: 561 388 129 402 20
Fragments arranged by size:
561 402 388 129 20
EcoO109I rG'GnC_Cy
Cuts at: 0 558 1351 1500
Size: 558 793 149
Fragments arranged by size:
793 558 149
EcoRI G'AATT_C
Cuts at: 0 787 1500
Size: 787 713
FseI GG_CCGG'CC
Cuts at: 0 104 1500
Size: 104 1396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 1235 1267 1500
Size: 391 32 812 32 233
Fragments arranged by size:
812 391 233 32 32
HincII GTy'rAC
Cuts at: 0 256 978 1173 1500
Size: 256 722 195 327
Fragments arranged by size:
722 327 256 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1500
Size: 1178 322
MslI CAynn'nnrTG
Cuts at: 0 152 220 293 1336 1500
Size: 152 68 73 1043 164
Fragments arranged by size:
1043 164 152 73 68
MspA1I CmG'CkG
Cuts at: 0 763 1500
Size: 763 737
NaeI GCC'GGC
Cuts at: 0 102 1500
Size: 102 1398
NgoMIV G'CCGG_C
Cuts at: 0 100 1500
Size: 100 1400
NspI r_CATG'y
Cuts at: 0 1235 1500
Size: 1235 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 592 1500
Size: 592 908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 728 760 1500
Size: 728 32 740
Fragments arranged by size:
740 728 32
PpuMI rG'GwC_Cy
Cuts at: 0 1351 1500
Size: 1351 149
PstI C_TGCA'G
Cuts at: 0 111 1500
Size: 111 1389
PvuII CAG'CTG
Cuts at: 0 763 1500
Size: 763 737
SalI G'TCGA_C
Cuts at: 0 976 1500
Size: 976 524
SfcI C'TryA_G
Cuts at: 0 107 235 1017 1500
Size: 107 128 782 483
Fragments arranged by size:
782 483 128 107
StyI C'CwwG_G
Cuts at: 0 1256 1500
Size: 1256 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1190 1500
Size: 1190 310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1360 1392 1500
Size: 1360 32 108
Fragments arranged by size:
1360 108 32
Tth111I GACn'n_nGTC
Cuts at: 0 1264 1500
Size: 1264 236
Enzymes that do cut:
AarI AccI AcuI AflIII AloI ApoI AvaI BaeI
BanII BbsI BcgI BclI BglI Bme1580I BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstBI BstXI BstYI Bsu36I BtgI DraIII
EarI Eco57MI EcoO109I EcoRI FseI Hin4I HincII MmeI
MslI MspA1I NaeI NgoMIV NspI PflMI PpiI PpuMI
PstI PvuII SalI SfcI StyI TaqII TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbvCI BciVI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRV FalI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XcmI
XhoI XmaI XmnI ZraI