Restriction map for
pFN21AE2474
(Linear) (Six-base) MAPSORT of: KIEE2474.seq Check: 1287 from: 1 to: 3999
KIEE2474.orf
With 182 enzymes: SgfI *
June 25, 2013 10:30 ..
AccI GT'mk_AC
Cuts at: 0 2296 3999
Size: 2296 1703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1555 2158 2577 3444 3503 3999
Size: 1555 603 419 867 59 496
Fragments arranged by size:
1555 867 603 496 419 59
AfeI AGC'GCT
Cuts at: 0 3249 3999
Size: 3249 750
AflIII A'CryG_T
Cuts at: 0 1454 3999
Size: 1454 2545
AhdI GACnn_n'nnGTC
Cuts at: 0 3268 3999
Size: 3268 731
AleI CACnn'nnGTG
Cuts at: 0 2422 2434 3665 3999
Size: 2422 12 1231 334
Fragments arranged by size:
2422 1231 334 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 175 263 3999
Size: 175 88 3736
Fragments arranged by size:
3736 175 88
AlwNI CAG_nnn'CTG
Cuts at: 0 1353 1578 2903 3999
Size: 1353 225 1325 1096
Fragments arranged by size:
1353 1325 1096 225
ApaI G_GGCC'C
Cuts at: 0 1842 2753 3999
Size: 1842 911 1246
Fragments arranged by size:
1842 1246 911
ApaLI G'TGCA_C
Cuts at: 0 1524 3999
Size: 1524 2475
ApoI r'AATT_y
Cuts at: 0 861 1943 3999
Size: 861 1082 2056
Fragments arranged by size:
2056 1082 861
AvaI C'yCGr_G
Cuts at: 0 394 1835 3646 3845 3999
Size: 394 1441 1811 199 154
Fragments arranged by size:
1811 1441 394 199 154
BamHI G'GATC_C
Cuts at: 0 612 848 3999
Size: 612 236 3151
Fragments arranged by size:
3151 612 236
BanI G'GyrC_C
Cuts at: 0 1132 1689 3999
Size: 1132 557 2310
Fragments arranged by size:
2310 1132 557
BanII G_rGCy'C
Cuts at: 0 401 629 1198 1842 2288 2753 3060 3162
Size: 401 228 569 644 446 465 307 102
Cuts at: 3162 3852 3999
Size: 690 147
Fragments arranged by size:
690 644 569 465 446 401 307 228
147 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1604 2320 2563 3350 3999
Size: 1604 716 243 787 649
Fragments arranged by size:
1604 787 716 649 243
BbvCI CC'TCA_GC
Cuts at: 0 649 1537 3999
Size: 649 888 2462
Fragments arranged by size:
2462 888 649
BciVI GTATCCnnnnn_n'
Cuts at: 0 701 3999
Size: 701 3298
BfrBI ATG'CAT
Cuts at: 0 2477 3766 3999
Size: 2477 1289 233
Fragments arranged by size:
2477 1289 233
BglI GCCn_nnn'nGGC
Cuts at: 0 220 1105 3999
Size: 220 885 2894
Fragments arranged by size:
2894 885 220
BlpI GC'TnA_GC
Cuts at: 0 749 950 1192 3851 3999
Size: 749 201 242 2659 148
Fragments arranged by size:
2659 749 242 201 148
Bme1580I G_kGCm'C
Cuts at: 0 1528 1694 1842 2692 2753 3218 3999
Size: 1528 166 148 850 61 465 781
Fragments arranged by size:
1528 850 781 465 166 148 61
BmgBI CAC'GTC
Cuts at: 0 704 1455 3999
Size: 704 751 2544
Fragments arranged by size:
2544 751 704
BmrI ACTGGGnnnn_n'
Cuts at: 0 327 2570 2702 3323 3357 3401 3999
Size: 327 2243 132 621 34 44 598
Fragments arranged by size:
2243 621 598 327 132 44 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1269 3453 3999
Size: 1269 2184 546
Fragments arranged by size:
2184 1269 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1012 2254 3537 3999
Size: 454 558 1242 1283 462
Fragments arranged by size:
1283 1242 558 462 454
Bpu10I CC'TnA_GC
Cuts at: 0 649 854 913 1537 2132 3999
Size: 649 205 59 624 595 1867
Fragments arranged by size:
1867 649 624 595 205 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 727 1384 2599 2899 3999
Size: 709 18 657 1215 300 1100
Fragments arranged by size:
1215 1100 709 657 300 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 2401 3999
Size: 2401 1598
BsaBI GATnn'nnATC
Cuts at: 0 3187 3999
Size: 3187 812
BsaHI Gr'CG_yC
Cuts at: 0 761 1036 3999
Size: 761 275 2963
Fragments arranged by size:
2963 761 275
BsaWI w'CCGG_w
Cuts at: 0 383 479 1520 2357 3999
Size: 383 96 1041 837 1642
Fragments arranged by size:
1642 1041 837 383 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 874 904 1231 1261 2704 2734
Size: 452 30 392 30 327 30 1443 30
Cuts at: 2734 3999
Size: 1265
Fragments arranged by size:
1443 1265 452 392 327 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 424 527 610 656 1216 1453 1489 1723
Size: 424 103 83 46 560 237 36 234
Cuts at: 1723 2402 2833 3643 3999
Size: 679 431 810 356
Fragments arranged by size:
810 679 560 431 424 356 237 234
103 83 46 36
BseYI C'CCAG_C
Cuts at: 0 840 1012 1578 1662 2903 3999
Size: 840 172 566 84 1241 1096
Fragments arranged by size:
1241 1096 840 566 172 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 330 432 1481 2229 3643 3860 3999
Size: 322 8 102 1049 748 1414 217 139
Fragments arranged by size:
1414 1049 748 322 217 139 102 8
BsiEI CG_ry'CG
Cuts at: 0 2463 3999
Size: 2463 1536
BsiHKAI G_wGCw'C
Cuts at: 0 1528 2225 2288 2419 3162 3852 3999
Size: 1528 697 63 131 743 690 147
Fragments arranged by size:
1528 743 697 690 147 131 63
BsmI GAATG_Cn'
Cuts at: 0 426 1258 2917 3999
Size: 426 832 1659 1082
Fragments arranged by size:
1659 1082 832 426
Bsp1286I G_dGCh'C
Cuts at: 0 401 629 1198 1528 1694 1842 2225 2288
Size: 401 228 569 330 166 148 383 63
Cuts at: 2288 2419 2692 2753 3060 3162 3218 3852 3999
Size: 131 273 61 307 102 56 634 147
Fragments arranged by size:
634 569 401 383 330 307 273 228
166 148 147 131 102 63 61 56
BspEI T'CCGG_A
Cuts at: 0 383 479 3999
Size: 383 96 3520
Fragments arranged by size:
3520 383 96
BspHI T'CATG_A
Cuts at: 0 362 2552 3999
Size: 362 2190 1447
Fragments arranged by size:
2190 1447 362
BsrDI GCAATG_nn'
Cuts at: 0 1289 2249 2604 2765 3927 3999
Size: 1289 960 355 161 1162 72
Fragments arranged by size:
1289 1162 960 355 161 72
BsrFI r'CCGG_y
Cuts at: 0 1724 2458 3999
Size: 1724 734 1541
Fragments arranged by size:
1724 1541 734
BsrGI T'GTAC_A
Cuts at: 0 371 2840 3071 3999
Size: 371 2469 231 928
Fragments arranged by size:
2469 928 371 231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 300 3926 3999
Size: 300 3626 73
Fragments arranged by size:
3626 300 73
BstEII G'GTnAC_C
Cuts at: 0 1299 1824 3999
Size: 1299 525 2175
Fragments arranged by size:
2175 1299 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2436 3610 3999
Size: 2436 1174 389
Fragments arranged by size:
2436 1174 389
BstYI r'GATC_y
Cuts at: 0 60 612 848 1223 2616 3686 3914 3999
Size: 60 552 236 375 1393 1070 228 85
Fragments arranged by size:
1393 1070 552 375 236 228 85 60
BtgI C'CryG_G
Cuts at: 0 324 404 2429 3099 3230 3666 3999
Size: 324 80 2025 670 131 436 333
Fragments arranged by size:
2025 670 436 333 324 131 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2487 3999
Size: 2487 1512
BtsI GCAGTG_nn'
Cuts at: 0 257 376 1058 2241 2441 2801 3999
Size: 257 119 682 1183 200 360 1198
Fragments arranged by size:
1198 1183 682 360 257 200 119
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2997 3032 3999
Size: 2997 35 967
Fragments arranged by size:
2997 967 35
DraIII CAC_nnn'GTG
Cuts at: 0 3950 3999
Size: 3950 49
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 1122 3999
Size: 562 560 2877
Fragments arranged by size:
2877 562 560
EaeI y'GGCC_r
Cuts at: 0 1097 1376 2214 2460 2531 3149 3999
Size: 1097 279 838 246 71 618 850
Fragments arranged by size:
1097 850 838 618 279 246 71
EagI C'GGCC_G
Cuts at: 0 2460 3999
Size: 2460 1539
EarI CTCTTCn'nnn_
Cuts at: 0 1684 2371 2938 3622 3799 3886 3999
Size: 1684 687 567 684 177 87 113
Fragments arranged by size:
1684 687 684 567 177 113 87
EcoICRI GAG'CTC
Cuts at: 0 2286 3160 3850 3999
Size: 2286 874 690 149
Fragments arranged by size:
2286 874 690 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1012 1555 2158 2254 2577 3444 3503
Size: 454 558 543 603 96 323 867 59
Cuts at: 3503 3537 3999
Size: 34 462
Fragments arranged by size:
867 603 558 543 462 454 323 96
59 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 489 1233 2161 3999
Size: 489 744 928 1838
Fragments arranged by size:
1838 928 744 489
EcoO109I rG'GnC_Cy
Cuts at: 0 987 2154 2749 2750 2853 3606 3999
Size: 987 1167 595 1 103 753 393
Fragments arranged by size:
1167 987 753 595 393 103 1
EcoRI G'AATT_C
Cuts at: 0 861 3999
Size: 861 3138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2565 3897 3999
Size: 2565 1332 102
Fragments arranged by size:
2565 1332 102
FseI GG_CCGG'CC
Cuts at: 0 1728 3999
Size: 1728 2271
HaeII r_GCGC'y
Cuts at: 0 3251 3999
Size: 3251 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 452 484 874 906 1037 1069
Size: 279 32 141 32 390 32 131 32
Cuts at: 1069 3254 3286 3535 3567 3999
Size: 2185 32 249 32 432
Fragments arranged by size:
2185 432 390 279 249 141 131 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2955 3999
Size: 2955 1044
HindIII A'AGCT_T
Cuts at: 0 952 2875 3024 3999
Size: 952 1923 149 975
Fragments arranged by size:
1923 975 952 149
MfeI C'AATT_G
Cuts at: 0 2093 3999
Size: 2093 1906
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1023 1100 2747 2959 3759 3999
Size: 640 383 77 1647 212 800 240
Fragments arranged by size:
1647 800 640 383 240 212 77
MscI TGG'CCA
Cuts at: 0 1099 1378 2216 2533 3151 3999
Size: 1099 279 838 317 618 848
Fragments arranged by size:
1099 848 838 618 317 279
MslI CAynn'nnrTG
Cuts at: 0 157 361 367 799 2422 2434 3665 3999
Size: 157 204 6 432 1623 12 1231 334
Fragments arranged by size:
1623 1231 432 334 204 157 12 6
MspA1I CmG'CkG
Cuts at: 0 446 678 999 1747 1912 3358 3999
Size: 446 232 321 748 165 1446 641
Fragments arranged by size:
1446 748 641 446 321 232 165
NaeI GCC'GGC
Cuts at: 0 1726 3999
Size: 1726 2273
NcoI C'CATG_G
Cuts at: 0 404 3999
Size: 404 3595
NgoMIV G'CCGG_C
Cuts at: 0 1724 3999
Size: 1724 2275
NsiI A_TGCA'T
Cuts at: 0 2479 3768 3999
Size: 2479 1289 231
Fragments arranged by size:
2479 1289 231
NspI r_CATG'y
Cuts at: 0 1755 2481 2502 3999
Size: 1755 726 21 1497
Fragments arranged by size:
1755 1497 726 21
PasI CC'CwG_GG
Cuts at: 0 2754 3999
Size: 2754 1245
PflMI CCAn_nnn'nTGG
Cuts at: 0 1430 2903 3999
Size: 1430 1473 1096
Fragments arranged by size:
1473 1430 1096
PfoI T'CCnGG_A
Cuts at: 0 989 1273 3999
Size: 989 284 2726
Fragments arranged by size:
2726 989 284
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 790 822 3999
Size: 790 32 3177
Fragments arranged by size:
3177 790 32
PpuMI rG'GwC_Cy
Cuts at: 0 987 2154 2853 3999
Size: 987 1167 699 1146
Fragments arranged by size:
1167 1146 987 699
PspOMI G'GGCC_C
Cuts at: 0 1838 2749 3999
Size: 1838 911 1250
Fragments arranged by size:
1838 1250 911
PspXI vC'TCGA_Gb
Cuts at: 0 3845 3999
Size: 3845 154
PstI C_TGCA'G
Cuts at: 0 185 1451 2817 3999
Size: 185 1266 1366 1182
Fragments arranged by size:
1366 1266 1182 185
PvuII CAG'CTG
Cuts at: 0 678 999 3358 3999
Size: 678 321 2359 641
Fragments arranged by size:
2359 678 641 321
SacI G_AGCT'C
Cuts at: 0 2288 3162 3852 3999
Size: 2288 874 690 147
Fragments arranged by size:
2288 874 690 147
SapI GCTCTTCn'nnn_
Cuts at: 0 1684 3999
Size: 1684 2315
SfcI C'TryA_G
Cuts at: 0 181 456 1176 1230 1447 2813 3420 3999
Size: 181 275 720 54 217 1366 607 579
Fragments arranged by size:
1366 720 607 579 275 217 181 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1105 3999
Size: 1105 2894
SmaI CCC'GGG
Cuts at: 0 1837 3999
Size: 1837 2162
SmlI C'TyrA_G
Cuts at: 0 724 742 1399 2578 2878 3845 3999
Size: 724 18 657 1179 300 967 154
Fragments arranged by size:
1179 967 724 657 300 154 18
SphI G_CATG'C
Cuts at: 0 2481 3999
Size: 2481 1518
StuI AGG'CCT
Cuts at: 0 715 1270 1759 3125 3999
Size: 715 555 489 1366 874
Fragments arranged by size:
1366 874 715 555 489
StyI C'CwwG_G
Cuts at: 0 404 429 1295 1694 2049 3120 3609 3999
Size: 404 25 866 399 355 1071 489 390
Fragments arranged by size:
1071 866 489 404 399 390 355 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2626 3423 3999
Size: 2626 797 576
Fragments arranged by size:
2626 797 576
TatI w'GTAC_w
Cuts at: 0 371 1775 2840 3071 3999
Size: 371 1404 1065 231 928
Fragments arranged by size:
1404 1065 928 371 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1507 1539 1588 1620 3999
Size: 1507 32 49 32 2379
Fragments arranged by size:
2379 1507 49 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3349 3999
Size: 3349 650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 771 3999
Size: 216 555 3228
Fragments arranged by size:
3228 555 216
XhoI C'TCGA_G
Cuts at: 0 3845 3999
Size: 3845 154
XmaI C'CCGG_G
Cuts at: 0 1835 3999
Size: 1835 2164
XmnI GAAnn'nnTTC
Cuts at: 0 424 3448 3999
Size: 424 3024 551
Fragments arranged by size:
3024 551 424
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SapI SfcI SfiI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BclI BglII
BmtI BsaAI BsiWI BsmBI BspMI BsrBI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraI EciI EcoRV FspI
FspAI HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI PacI PciI PmeI PmlI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI ZraI