Restriction map for
pFN21AE2470
(Linear) (Six-base) MAPSORT of: KIEE2470.seq Check: 6406 from: 1 to: 1422
KIEE2470.orf
With 182 enzymes: SgfI *
May 21, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 0 887 1422
Size: 887 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 1422
Size: 1248 174
AfeI AGC'GCT
Cuts at: 0 38 839 1422
Size: 38 801 583
Fragments arranged by size:
801 583 38
AflIII A'CryG_T
Cuts at: 0 1130 1422
Size: 1130 292
AlwNI CAG_nnn'CTG
Cuts at: 0 691 1422
Size: 691 731
AvaI C'yCGr_G
Cuts at: 0 63 76 144 162 849 1173 1354 1374
Size: 63 13 68 18 687 324 181 20
Cuts at: 1374 1387 1422
Size: 13 35
Fragments arranged by size:
687 324 181 68 63 35 20 18
13 13
BanI G'GyrC_C
Cuts at: 0 67 562 751 1422
Size: 67 495 189 671
Fragments arranged by size:
671 495 189 67
BanII G_rGCy'C
Cuts at: 0 58 1180 1313 1422
Size: 58 1122 133 109
Fragments arranged by size:
1122 133 109 58
BbeI G_GCGC'C
Cuts at: 0 71 566 755 1422
Size: 71 495 189 667
Fragments arranged by size:
667 495 189 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 1211 1422
Size: 1211 211
BglI GCCn_nnn'nGGC
Cuts at: 0 500 721 817 1019 1422
Size: 500 221 96 202 403
Fragments arranged by size:
500 403 221 202 96
BglII A'GATC_T
Cuts at: 0 263 1422
Size: 263 1159
Bme1580I G_kGCm'C
Cuts at: 0 173 910 1422
Size: 173 737 512
Fragments arranged by size:
737 512 173
BmgBI CAC'GTC
Cuts at: 0 911 1422
Size: 911 511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1332 1389 1402 1422
Size: 1332 57 13 20
Fragments arranged by size:
1332 57 20 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1422
Size: 405 1017
Bpu10I CC'TnA_GC
Cuts at: 0 1015 1422
Size: 1015 407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1422
Size: 334 1088
BsaHI Gr'CG_yC
Cuts at: 0 68 563 752 1422
Size: 68 495 189 670
Fragments arranged by size:
670 495 189 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 409 433 439 455 485 1120 1150
Size: 403 6 24 6 16 30 635 30
Cuts at: 1150 1422
Size: 272
Fragments arranged by size:
635 403 272 30 30 24 16 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 417 432 551 804 991 994 1128
Size: 84 333 15 119 253 187 3 134
Cuts at: 1128 1146 1149 1158 1161 1170 1422
Size: 18 3 9 3 9 252
Fragments arranged by size:
333 253 252 187 134 119 84 18
15 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 81 276 793 952 1009 1228 1422
Size: 81 195 517 159 57 219 194
Fragments arranged by size:
517 219 195 194 159 81 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1332 1422
Size: 25 1307 90
Fragments arranged by size:
1307 90 25
BsiEI CG_ry'CG
Cuts at: 0 434 1023 1056 1287 1422
Size: 434 589 33 231 135
Fragments arranged by size:
589 434 231 135 33
BsiHKAI G_wGCw'C
Cuts at: 0 58 1422
Size: 58 1364
Bsp1286I G_dGCh'C
Cuts at: 0 58 173 910 1180 1313 1422
Size: 58 115 737 270 133 109
Fragments arranged by size:
737 270 133 115 109 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1220 1422
Size: 1220 202
BsrBI CCG'CTC
Cuts at: 0 219 339 1019 1058 1422
Size: 219 120 680 39 364
Fragments arranged by size:
680 364 219 120 39
BsrFI r'CCGG_y
Cuts at: 0 46 70 383 576 1051 1422
Size: 46 24 313 193 475 371
Fragments arranged by size:
475 371 313 193 46 24
BsrGI T'GTAC_A
Cuts at: 0 776 1422
Size: 776 646
BssHII G'CGCG_C
Cuts at: 0 1092 1118 1422
Size: 1092 26 304
Fragments arranged by size:
1092 304 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 691 1422
Size: 691 731
BstBI TT'CG_AA
Cuts at: 0 1202 1422
Size: 1202 220
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 1422
Size: 276 1146
BstYI r'GATC_y
Cuts at: 0 263 314 1422
Size: 263 51 1108
Fragments arranged by size:
1108 263 51
BtgI C'CryG_G
Cuts at: 0 122 495 923 1074 1422
Size: 122 373 428 151 348
Fragments arranged by size:
428 373 348 151 122
BtsI GCAGTG_nn'
Cuts at: 0 486 1422
Size: 486 936
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 456 491 1422
Size: 456 35 931
Fragments arranged by size:
931 456 35
EaeI y'GGCC_r
Cuts at: 0 44 431 492 1020 1053 1422
Size: 44 387 61 528 33 369
Fragments arranged by size:
528 387 369 61 44 33
EagI C'GGCC_G
Cuts at: 0 431 1020 1053 1422
Size: 431 589 33 369
Fragments arranged by size:
589 431 369 33
EarI CTCTTCn'nnn_
Cuts at: 0 1167 1422
Size: 1167 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 798 880 1422
Size: 349 449 82 542
Fragments arranged by size:
542 449 349 82
EcoICRI GAG'CTC
Cuts at: 0 56 1422
Size: 56 1366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1248 1422
Size: 405 843 174
Fragments arranged by size:
843 405 174
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1422
Size: 973 449
EcoO109I rG'GnC_Cy
Cuts at: 0 1290 1422
Size: 1290 132
HaeII r_GCGC'y
Cuts at: 0 40 71 566 755 800 841 1034 1282
Size: 40 31 495 189 45 41 193 248
Cuts at: 1282 1422
Size: 140
Fragments arranged by size:
495 248 193 189 140 45 41 40
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 754 786 1234 1266 1422
Size: 455 32 267 32 448 32 156
Fragments arranged by size:
455 448 267 156 32 32 32
KasI G'GCGC_C
Cuts at: 0 67 562 751 1422
Size: 67 495 189 671
Fragments arranged by size:
671 495 189 67
MluI A'CGCG_T
Cuts at: 0 1130 1422
Size: 1130 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 1422
Size: 1214 208
MspA1I CmG'CkG
Cuts at: 0 688 1076 1422
Size: 688 388 346
Fragments arranged by size:
688 388 346
NaeI GCC'GGC
Cuts at: 0 48 72 1053 1422
Size: 48 24 981 369
Fragments arranged by size:
981 369 48 24
NarI GG'CG_CC
Cuts at: 0 68 563 752 1422
Size: 68 495 189 670
Fragments arranged by size:
670 495 189 68
NcoI C'CATG_G
Cuts at: 0 495 1422
Size: 495 927
NgoMIV G'CCGG_C
Cuts at: 0 46 70 1051 1422
Size: 46 24 981 371
Fragments arranged by size:
981 371 46 24
NotI GC'GGCC_GC
Cuts at: 0 1020 1422
Size: 1020 402
NspI r_CATG'y
Cuts at: 0 252 1251 1422
Size: 252 999 171
Fragments arranged by size:
999 252 171
PasI CC'CwG_GG
Cuts at: 0 747 963 1254 1422
Size: 747 216 291 168
Fragments arranged by size:
747 291 216 168
PfoI T'CCnGG_A
Cuts at: 0 1337 1422
Size: 1337 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1422
Size: 1216 32 174
Fragments arranged by size:
1216 174 32
PpuMI rG'GwC_Cy
Cuts at: 0 1290 1422
Size: 1290 132
PspXI vC'TCGA_Gb
Cuts at: 0 76 1387 1422
Size: 76 1311 35
Fragments arranged by size:
1311 76 35
SacI G_AGCT'C
Cuts at: 0 58 1422
Size: 58 1364
SacII CC_GC'GG
Cuts at: 0 1077 1422
Size: 1077 345
SexAI A'CCwGG_T
Cuts at: 0 1406 1422
Size: 1406 16
SfoI GGC'GCC
Cuts at: 0 69 564 753 1422
Size: 69 495 189 669
Fragments arranged by size:
669 495 189 69
SmaI CCC'GGG
Cuts at: 0 851 1422
Size: 851 571
SmlI C'TyrA_G
Cuts at: 0 76 349 1387 1422
Size: 76 273 1038 35
Fragments arranged by size:
1038 273 76 35
StyI C'CwwG_G
Cuts at: 0 495 1422
Size: 495 927
TatI w'GTAC_w
Cuts at: 0 776 1422
Size: 776 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1422
Size: 1120 32 270
Fragments arranged by size:
1120 270 32
Tth111I GACn'n_nGTC
Cuts at: 0 469 1422
Size: 469 953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 960 1422
Size: 654 306 462
Fragments arranged by size:
654 462 306
XhoI C'TCGA_G
Cuts at: 0 76 1387 1422
Size: 76 1311 35
Fragments arranged by size:
1311 76 35
XmaI C'CCGG_G
Cuts at: 0 849 1422
Size: 849 573
XmnI GAAnn'nnTTC
Cuts at: 0 193 1422
Size: 193 1229
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstXI BstYI BtgI BtsI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MluI MmeI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PfoI PpiI PpuMI PspXI SacI
SacII SexAI SfoI SmaI SmlI StyI TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BssSI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MscI MslI NdeI NheI NruI NsiI PacI
PciI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII XbaI ZraI