(Linear) (Six-base) MAPSORT of: KIEE2458.seq  Check: 3525  from: 1  to: 828



KIEE2458.orf

 With 182 enzymes: SgfI * 

                        February 8, 2013 18:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     605     828 
   Size:        523      82     223

  Fragments arranged by size: 

                 523     223      82
 
AfeI AGC'GCT

Cuts at:      0      67     828 
   Size:         67     761
 
AflIII A'CryG_T

Cuts at:      0     128     828 
   Size:        128     700
 
AlwNI CAG_nnn'CTG

Cuts at:      0     382     828 
   Size:        382     446
 
ApoI r'AATT_y

Cuts at:      0     171     779     828 
   Size:        171     608      49

  Fragments arranged by size: 

                 608     171      49
 
AvaI C'yCGr_G

Cuts at:      0      11     828 
   Size:         11     817
 
BanI G'GyrC_C

Cuts at:      0     463     828 
   Size:        463     365
 
BanII G_rGCy'C

Cuts at:      0     389     648     828 
   Size:        389     259     180

  Fragments arranged by size: 

                 389     259     180
 
BlpI GC'TnA_GC

Cuts at:      0     383     828 
   Size:        383     445
 
BmgBI CAC'GTC

Cuts at:      0     762     828 
   Size:        762      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151     828 
   Size:        151     677
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     398     828 
   Size:        398     430
 
BsaHI Gr'CG_yC

Cuts at:      0      38     828 
   Size:         38     790
 
BseYI C'CCAG_C

Cuts at:      0     703     828 
   Size:        703     125
 
BsiHKAI G_wGCw'C

Cuts at:      0     389     828 
   Size:        389     439
 
BsmI GAATG_Cn'

Cuts at:      0     230     828 
   Size:        230     598
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     648     828 
   Size:        389     259     180

  Fragments arranged by size: 

                 389     259     180
 
BspHI T'CATG_A

Cuts at:      0     161     828 
   Size:        161     667
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      45     828 
   Size:         45     783
 
BstYI r'GATC_y

Cuts at:      0     507     828 
   Size:        507     321
 
BtsI GCAGTG_nn'

Cuts at:      0     369     753     828 
   Size:        369     384      75

  Fragments arranged by size: 

                 384     369      75
 
DraI TTT'AAA

Cuts at:      0     564     828 
   Size:        564     264
 
DraIII CAC_nnn'GTG

Cuts at:      0     183     828 
   Size:        183     645
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     542     828 
   Size:         92     450     286

  Fragments arranged by size: 

                 450     286      92
 
EcoICRI GAG'CTC

Cuts at:      0     387     828 
   Size:        387     441
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     605     828 
   Size:        523      82     223

  Fragments arranged by size: 

                 523     223      82
 
HaeII r_GCGC'y

Cuts at:      0      33      69     828 
   Size:         33      36     759

  Fragments arranged by size: 

                 759      36      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     519     551     828 
   Size:        519      32     277

  Fragments arranged by size: 

                 519     277      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     485     828 
   Size:        485     343
 
MslI CAynn'nnrTG

Cuts at:      0     133     160     235     469     828 
   Size:        133      27      75     234     359

  Fragments arranged by size: 

                 359     234     133      75      27
 
MspA1I CmG'CkG

Cuts at:      0      43     379     713     828 
   Size:         43     336     334     115

  Fragments arranged by size: 

                 336     334     115      43
 
NspI r_CATG'y

Cuts at:      0     132     828 
   Size:        132     696
 
PciI A'CATG_T

Cuts at:      0     128     828 
   Size:        128     700
 
PvuII CAG'CTG

Cuts at:      0     379     828 
   Size:        379     449
 
SacI G_AGCT'C

Cuts at:      0     389     828 
   Size:        389     439
 
SapI GCTCTTCn'nnn_

Cuts at:      0     542     828 
   Size:        542     286
 
SmaI CCC'GGG

Cuts at:      0      13     828 
   Size:         13     815
 
Tth111I GACn'n_nGTC

Cuts at:      0     157     828 
   Size:        157     671
 
XmaI C'CCGG_G

Cuts at:      0      11     828 
   Size:         11     817
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     828 
   Size:        278     550
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     ApoI     AvaI     BanI    BanII
     BlpI    BmgBI     BmrI     BsaI    BsaHI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BstYI     BtsI     DraI   DraIII     EarI
  EcoICRI  Eco57MI    HaeII    Hin4I     MmeI     MslI   MspA1I     NspI
     PciI    PvuII     SacI     SapI     SmaI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
     XbaI     XcmI     XhoI     ZraI