Restriction map for
pFN21AE2452
(Linear) (Six-base) MAPSORT of: KIEE2452.seq Check: 9694 from: 1 to: 1587
KIEE2452.orf
With 182 enzymes: SgfI *
February 5, 2013 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 61 1331 1445 1587
Size: 61 1270 114 142
Fragments arranged by size:
1270 142 114 61
AatII G_ACGT'C
Cuts at: 0 560 764 1587
Size: 560 204 823
Fragments arranged by size:
823 560 204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 685 1204 1587
Size: 224 461 519 383
Fragments arranged by size:
519 461 383 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 922 1442 1587
Size: 922 520 145
Fragments arranged by size:
922 520 145
ApaI G_GGCC'C
Cuts at: 0 7 1107 1587
Size: 7 1100 480
Fragments arranged by size:
1100 480 7
ApaLI G'TGCA_C
Cuts at: 0 511 1054 1417 1587
Size: 511 543 363 170
Fragments arranged by size:
543 511 363 170
AscI GG'CGCG_CC
Cuts at: 0 220 1587
Size: 220 1367
BamHI G'GATC_C
Cuts at: 0 422 1587
Size: 422 1165
BanI G'GyrC_C
Cuts at: 0 228 519 712 1300 1587
Size: 228 291 193 588 287
Fragments arranged by size:
588 291 287 228 193
BanII G_rGCy'C
Cuts at: 0 7 108 544 1107 1166 1587
Size: 7 101 436 563 59 421
Fragments arranged by size:
563 436 421 101 59 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 849 918 1032 1587
Size: 849 69 114 555
Fragments arranged by size:
849 555 114 69
BbvCI CC'TCA_GC
Cuts at: 0 1352 1587
Size: 1352 235
BglI GCCn_nnn'nGGC
Cuts at: 0 1128 1587
Size: 1128 459
BglII A'GATC_T
Cuts at: 0 749 1587
Size: 749 838
BlpI GC'TnA_GC
Cuts at: 0 40 86 278 1189 1587
Size: 40 46 192 911 398
Fragments arranged by size:
911 398 192 46 40
Bme1580I G_kGCm'C
Cuts at: 0 7 233 508 515 524 715 1058 1107
Size: 7 226 275 7 9 191 343 49
Cuts at: 1107 1124 1303 1421 1587
Size: 17 179 118 166
Fragments arranged by size:
343 275 226 191 179 166 118 49
17 9 7 7
BmgBI CAC'GTC
Cuts at: 0 246 1587
Size: 246 1341
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 525 684 1101 1587
Size: 125 400 159 417 486
Fragments arranged by size:
486 417 400 159 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 1587
Size: 136 1451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 658 1231 1587
Size: 131 527 573 356
Fragments arranged by size:
573 527 356 131
Bpu10I CC'TnA_GC
Cuts at: 0 1352 1587
Size: 1352 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 1587
Size: 107 1480
BsaBI GATnn'nnATC
Cuts at: 0 421 1587
Size: 421 1166
BsaHI Gr'CG_yC
Cuts at: 0 557 761 1587
Size: 557 204 826
Fragments arranged by size:
826 557 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 481 511 526 556 853 883
Size: 106 30 345 30 15 30 297 30
Cuts at: 883 1048 1078 1587
Size: 165 30 509
Fragments arranged by size:
509 345 297 165 106 30 30 30
30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 1246 1267 1270 1587
Size: 114 1132 21 3 317
Fragments arranged by size:
1132 317 114 21 3
BseYI C'CCAG_C
Cuts at: 0 66 215 1069 1587
Size: 66 149 854 518
Fragments arranged by size:
854 518 149 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 1309 1587
Size: 1295 14 278
Fragments arranged by size:
1295 278 14
BsiHKAI G_wGCw'C
Cuts at: 0 515 544 1049 1058 1166 1421 1587
Size: 515 29 505 9 108 255 166
Fragments arranged by size:
515 505 255 166 108 29 9
Bsp1286I G_dGCh'C
Cuts at: 0 7 108 233 508 515 524 544 715
Size: 7 101 125 275 7 9 20 171
Cuts at: 715 1049 1058 1107 1124 1166 1303 1421 1587
Size: 334 9 49 17 42 137 118 166
Fragments arranged by size:
334 275 171 166 137 125 118 101
49 42 20 17 9 9 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 872 1001 1267 1331 1445 1587
Size: 61 811 129 266 64 114 142
Fragments arranged by size:
811 266 142 129 114 64 61
BsrBI CCG'CTC
Cuts at: 0 683 795 978 1064 1587
Size: 683 112 183 86 523
Fragments arranged by size:
683 523 183 112 86
BsrDI GCAATG_nn'
Cuts at: 0 142 1424 1587
Size: 142 1282 163
Fragments arranged by size:
1282 163 142
BsrFI r'CCGG_y
Cuts at: 0 179 1142 1587
Size: 179 963 445
Fragments arranged by size:
963 445 179
BssHII G'CGCG_C
Cuts at: 0 220 1587
Size: 220 1367
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 1307 1587
Size: 519 788 280
Fragments arranged by size:
788 519 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1587
Size: 966 621
BstYI r'GATC_y
Cuts at: 0 422 749 1505 1587
Size: 422 327 756 82
Fragments arranged by size:
756 422 327 82
Bsu36I CC'TnA_GG
Cuts at: 0 1338 1587
Size: 1338 249
BtgI C'CryG_G
Cuts at: 0 137 500 707 1157 1314 1490 1587
Size: 137 363 207 450 157 176 97
Fragments arranged by size:
450 363 207 176 157 137 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 258 1587
Size: 258 1329
BtsI GCAGTG_nn'
Cuts at: 0 473 1587
Size: 473 1114
ClaI AT'CG_AT
Cuts at: 0 287 1587
Size: 287 1300
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 360 395 1587
Size: 360 35 1192
Fragments arranged by size:
1192 360 35
DraIII CAC_nnn'GTG
Cuts at: 0 519 896 1095 1587
Size: 519 377 199 492
Fragments arranged by size:
519 492 377 199
EaeI y'GGCC_r
Cuts at: 0 527 1317 1587
Size: 527 790 270
Fragments arranged by size:
790 527 270
EarI CTCTTCn'nnn_
Cuts at: 0 190 202 499 802 1464 1587
Size: 190 12 297 303 662 123
Fragments arranged by size:
662 303 297 190 123 12
EcoICRI GAG'CTC
Cuts at: 0 542 1164 1587
Size: 542 622 423
Fragments arranged by size:
622 542 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 224 658 685 1204 1231 1587
Size: 131 93 434 27 519 27 356
Fragments arranged by size:
519 434 356 131 93 27 27
EcoO109I rG'GnC_Cy
Cuts at: 0 4 402 1103 1104 1334 1342 1587
Size: 4 398 701 1 230 8 245
Fragments arranged by size:
701 398 245 230 8 4 1
HaeII r_GCGC'y
Cuts at: 0 93 1587
Size: 93 1494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 257 289 1441 1473 1587
Size: 106 32 119 32 1152 32 114
Fragments arranged by size:
1152 119 114 106 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1587
Size: 896 691
MslI CAynn'nnrTG
Cuts at: 0 289 904 1587
Size: 289 615 683
Fragments arranged by size:
683 615 289
MspA1I CmG'CkG
Cuts at: 0 215 1587
Size: 215 1372
NcoI C'CATG_G
Cuts at: 0 1490 1587
Size: 1490 97
NspI r_CATG'y
Cuts at: 0 1274 1398 1587
Size: 1274 124 189
Fragments arranged by size:
1274 189 124
PasI CC'CwG_GG
Cuts at: 0 101 1587
Size: 101 1486
PflMI CCAn_nnn'nTGG
Cuts at: 0 73 326 1587
Size: 73 253 1261
Fragments arranged by size:
1261 253 73
PfoI T'CCnGG_A
Cuts at: 0 108 607 1587
Size: 108 499 980
Fragments arranged by size:
980 499 108
PpuMI rG'GwC_Cy
Cuts at: 0 402 1342 1587
Size: 402 940 245
Fragments arranged by size:
940 402 245
PspOMI G'GGCC_C
Cuts at: 0 3 1103 1587
Size: 3 1100 484
Fragments arranged by size:
1100 484 3
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 982 1014 1587
Size: 982 32 573
Fragments arranged by size:
982 573 32
PstI C_TGCA'G
Cuts at: 0 550 1312 1367 1587
Size: 550 762 55 220
Fragments arranged by size:
762 550 220 55
SacI G_AGCT'C
Cuts at: 0 544 1166 1587
Size: 544 622 421
Fragments arranged by size:
622 544 421
SanDI GG'GwC_CC
Cuts at: 0 402 1587
Size: 402 1185
SapI GCTCTTCn'nnn_
Cuts at: 0 190 802 1587
Size: 190 612 785
Fragments arranged by size:
785 612 190
SfcI C'TryA_G
Cuts at: 0 546 1308 1363 1587
Size: 546 762 55 224
Fragments arranged by size:
762 546 224 55
StyI C'CwwG_G
Cuts at: 0 482 1346 1490 1587
Size: 482 864 144 97
Fragments arranged by size:
864 482 144 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 507 1083 1090 1587
Size: 507 576 7 497
Fragments arranged by size:
576 507 497 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 1048 1080 1587
Size: 481 32 535 32 507
Fragments arranged by size:
535 507 481 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 633 1327 1587
Size: 633 694 260
Fragments arranged by size:
694 633 260
ZraI GAC'GTC
Cuts at: 0 558 762 1587
Size: 558 204 825
Fragments arranged by size:
825 558 204
Enzymes that do cut:
AarI AatII AcuI AlfI ApaI ApaLI AscI BamHI
BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I MmeI MslI MspA1I NcoI
NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI
SacI SanDI SapI SfcI StyI TaqII TstI XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApoI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BmtI BpuEI
BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssSI BstBI BstEII BstZ17I DraI DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI