Restriction map for
pFN21AE2451
(Linear) (Six-base) MAPSORT of: KIEE2451.seq Check: 4656 from: 1 to: 1134
KIEE2451.orf
With 182 enzymes: SgfI *
February 5, 2013 10:21 ..
AccI GT'mk_AC
Cuts at: 0 724 1134
Size: 724 410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 241 1134
Size: 53 188 893
Fragments arranged by size:
893 188 53
AflIII A'CryG_T
Cuts at: 0 584 794 1134
Size: 584 210 340
Fragments arranged by size:
584 340 210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 603 1134
Size: 361 242 531
Fragments arranged by size:
531 361 242
AlwNI CAG_nnn'CTG
Cuts at: 0 76 688 862 1134
Size: 76 612 174 272
Fragments arranged by size:
612 272 174 76
ApaI G_GGCC'C
Cuts at: 0 665 1134
Size: 665 469
AvaI C'yCGr_G
Cuts at: 0 270 643 1134
Size: 270 373 491
Fragments arranged by size:
491 373 270
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 842 875 1134
Size: 842 33 259
Fragments arranged by size:
842 259 33
BanII G_rGCy'C
Cuts at: 0 665 1134
Size: 665 469
BbsI GAAGACnn'nnnn_
Cuts at: 0 561 625 818 1115 1121 1134
Size: 561 64 193 297 6 13
Fragments arranged by size:
561 297 193 64 13 6
BbvCI CC'TCA_GC
Cuts at: 0 833 1134
Size: 833 301
BclI T'GATC_A
Cuts at: 0 950 1134
Size: 950 184
Bme1580I G_kGCm'C
Cuts at: 0 178 665 1134
Size: 178 487 469
Fragments arranged by size:
487 469 178
BmgBI CAC'GTC
Cuts at: 0 1092 1134
Size: 1092 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 299 692 1134
Size: 299 393 442
Fragments arranged by size:
442 393 299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1134
Size: 1033 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 729 860 1134
Size: 532 197 131 274
Fragments arranged by size:
532 274 197 131
Bpu10I CC'TnA_GC
Cuts at: 0 833 863 1134
Size: 833 30 271
Fragments arranged by size:
833 271 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1134
Size: 216 918
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 1134
Size: 140 994
BsaAI yAC'GTr
Cuts at: 0 587 795 1134
Size: 587 208 339
Fragments arranged by size:
587 339 208
BsaBI GATnn'nnATC
Cuts at: 0 952 1134
Size: 952 182
BsaWI w'CCGG_w
Cuts at: 0 184 535 615 1134
Size: 184 351 80 519
Fragments arranged by size:
519 351 184 80
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 821 1134
Size: 821 313
BseYI C'CCAG_C
Cuts at: 0 158 489 1134
Size: 158 331 645
Fragments arranged by size:
645 331 158
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1114 1134
Size: 479 635 20
Fragments arranged by size:
635 479 20
BsiHKAI G_wGCw'C
Cuts at: 0 980 1134
Size: 980 154
Bsp1286I G_dGCh'C
Cuts at: 0 178 665 980 1134
Size: 178 487 315 154
Fragments arranged by size:
487 315 178 154
BspEI T'CCGG_A
Cuts at: 0 615 1134
Size: 615 519
BsrDI GCAATG_nn'
Cuts at: 0 560 1134
Size: 560 574
BstEII G'GTnAC_C
Cuts at: 0 97 225 1134
Size: 97 128 909
Fragments arranged by size:
909 128 97
BstYI r'GATC_y
Cuts at: 0 774 1134
Size: 774 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 939 1134
Size: 939 195
BtsI GCAGTG_nn'
Cuts at: 0 671 1134
Size: 671 463
DraIII CAC_nnn'GTG
Cuts at: 0 105 1134
Size: 105 1029
EarI CTCTTCn'nnn_
Cuts at: 0 985 1134
Size: 985 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 241 532 729 860 1134
Size: 53 188 291 197 131 274
Fragments arranged by size:
291 274 197 188 131 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 616 1134
Size: 616 518
EcoO109I rG'GnC_Cy
Cuts at: 0 661 1134
Size: 661 473
HaeII r_GCGC'y
Cuts at: 0 202 1134
Size: 202 932
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 766 798 808 840 962 994
Size: 266 32 468 32 10 32 122 32
Cuts at: 994 1134
Size: 140
Fragments arranged by size:
468 266 140 122 32 32 32 32
10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1134
Size: 526 608
MslI CAynn'nnrTG
Cuts at: 0 343 913 1134
Size: 343 570 221
Fragments arranged by size:
570 343 221
MspA1I CmG'CkG
Cuts at: 0 320 493 1134
Size: 320 173 641
Fragments arranged by size:
641 320 173
NspI r_CATG'y
Cuts at: 0 156 1134
Size: 156 978
PflMI CCAn_nnn'nTGG
Cuts at: 0 770 1134
Size: 770 364
PfoI T'CCnGG_A
Cuts at: 0 706 1134
Size: 706 428
PmlI CAC'GTG
Cuts at: 0 587 795 1134
Size: 587 208 339
Fragments arranged by size:
587 339 208
PshAI GACnn'nnGTC
Cuts at: 0 61 1134
Size: 61 1073
PspOMI G'GGCC_C
Cuts at: 0 661 1134
Size: 661 473
PstI C_TGCA'G
Cuts at: 0 678 1134
Size: 678 456
PvuII CAG'CTG
Cuts at: 0 493 1134
Size: 493 641
SapI GCTCTTCn'nnn_
Cuts at: 0 985 1134
Size: 985 149
SfcI C'TryA_G
Cuts at: 0 391 674 1134
Size: 391 283 460
Fragments arranged by size:
460 391 283
SmlI C'TyrA_G
Cuts at: 0 231 1134
Size: 231 903
StyI C'CwwG_G
Cuts at: 0 965 1134
Size: 965 169
TatI w'GTAC_w
Cuts at: 0 122 326 1134
Size: 122 204 808
Fragments arranged by size:
808 204 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 209 241 798 830 1134
Size: 209 32 557 32 304
Fragments arranged by size:
557 304 209 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 739 1134
Size: 739 395
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI AvaI BaeI
BanII BbsI BbvCI BclI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BsrDI BstEII BstYI
BtgZI BtsI DraIII EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MslI MspA1I NspI PflMI PfoI PmlI
PshAI PspOMI PstI PvuII SapI SfcI SmlI StyI
TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BanI BbeI BcgI BciVI BfrBI BglI BglII
BlpI BmtI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII XbaI XcmI XhoI XmaI XmnI
ZraI