Restriction map for
pFN21AE2448
(Linear) (Six-base) MAPSORT of: KIEE2448.seq Check: 293 from: 1 to: 1797
KIEE2448.orf
With 182 enzymes: SgfI *
January 7, 2013 14:39 ..
AatII G_ACGT'C
Cuts at: 0 275 1797
Size: 275 1522
AccI GT'mk_AC
Cuts at: 0 1469 1797
Size: 1469 328
Acc65I G'GTAC_C
Cuts at: 0 1382 1797
Size: 1382 415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1797
Size: 169 1628
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 78 110 1797
Size: 78 32 1687
Fragments arranged by size:
1687 78 32
AlwNI CAG_nnn'CTG
Cuts at: 0 297 863 1797
Size: 297 566 934
Fragments arranged by size:
934 566 297
ApaLI G'TGCA_C
Cuts at: 0 580 882 1797
Size: 580 302 915
Fragments arranged by size:
915 580 302
ApoI r'AATT_y
Cuts at: 0 550 1797
Size: 550 1247
BanI G'GyrC_C
Cuts at: 0 673 702 829 868 1144 1267 1382 1389
Size: 673 29 127 39 276 123 115 7
Cuts at: 1389 1607 1797
Size: 218 190
Fragments arranged by size:
673 276 218 190 127 123 115 39
29 7
BanII G_rGCy'C
Cuts at: 0 179 236 953 1258 1517 1682 1797
Size: 179 57 717 305 259 165 115
Fragments arranged by size:
717 305 259 179 165 115 57
BbeI G_GCGC'C
Cuts at: 0 706 872 1148 1611 1797
Size: 706 166 276 463 186
Fragments arranged by size:
706 463 276 186 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 1173 1797
Size: 1173 624
BbvCI CC'TCA_GC
Cuts at: 0 143 277 1797
Size: 143 134 1520
Fragments arranged by size:
1520 143 134
BclI T'GATC_A
Cuts at: 0 770 1220 1797
Size: 770 450 577
Fragments arranged by size:
770 577 450
BglI GCCn_nnn'nGGC
Cuts at: 0 1288 1620 1797
Size: 1288 332 177
Fragments arranged by size:
1288 332 177
BglII A'GATC_T
Cuts at: 0 1775 1797
Size: 1775 22
BlpI GC'TnA_GC
Cuts at: 0 443 1321 1797
Size: 443 878 476
Fragments arranged by size:
878 476 443
Bme1580I G_kGCm'C
Cuts at: 0 473 584 886 946 1202 1797
Size: 473 111 302 60 256 595
Fragments arranged by size:
595 473 302 256 111 60
BmgBI CAC'GTC
Cuts at: 0 410 1797
Size: 410 1387
BmrI ACTGGGnnnn_n'
Cuts at: 0 1261 1797
Size: 1261 536
BmtI G_CTAG'C
Cuts at: 0 667 1797
Size: 667 1130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 86 646 667 1696 1797
Size: 75 11 560 21 1029 101
Fragments arranged by size:
1029 560 101 75 21 11
Bpu10I CC'TnA_GC
Cuts at: 0 143 277 1797
Size: 143 134 1520
Fragments arranged by size:
1520 143 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 700 1797
Size: 205 495 1097
Fragments arranged by size:
1097 495 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 1797
Size: 525 1272
BsaHI Gr'CG_yC
Cuts at: 0 272 703 869 1145 1608 1797
Size: 272 431 166 276 463 189
Fragments arranged by size:
463 431 276 272 189 166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 405 1567 1797
Size: 226 179 1162 230
Fragments arranged by size:
1162 230 226 179
BseYI C'CCAG_C
Cuts at: 0 297 1107 1454 1462 1787 1797
Size: 297 810 347 8 325 10
Fragments arranged by size:
810 347 325 297 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 772 1379 1797
Size: 415 357 607 418
Fragments arranged by size:
607 418 415 357
BsiEI CG_ry'CG
Cuts at: 0 1056 1193 1615 1797
Size: 1056 137 422 182
Fragments arranged by size:
1056 422 182 137
BsiHKAI G_wGCw'C
Cuts at: 0 179 584 886 953 1004 1577 1797
Size: 179 405 302 67 51 573 220
Fragments arranged by size:
573 405 302 220 179 67 51
Bsp1286I G_dGCh'C
Cuts at: 0 179 236 473 584 886 946 953 1004
Size: 179 57 237 111 302 60 7 51
Cuts at: 1004 1202 1258 1517 1577 1682 1797
Size: 198 56 259 60 105 115
Fragments arranged by size:
302 259 237 198 179 115 111 105
60 60 57 56 51 7
BspHI T'CATG_A
Cuts at: 0 773 1797
Size: 773 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1323 1481 1797
Size: 1323 158 316
Fragments arranged by size:
1323 316 158
BsrBI CCG'CTC
Cuts at: 0 735 1797
Size: 735 1062
BsrFI r'CCGG_y
Cuts at: 0 187 208 985 1147 1366 1610 1614 1797
Size: 187 21 777 162 219 244 4 183
Fragments arranged by size:
777 244 219 187 183 162 21 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1721 1797
Size: 1721 76
BstEII G'GTnAC_C
Cuts at: 0 1437 1695 1797
Size: 1437 258 102
Fragments arranged by size:
1437 258 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 480 516 1416 1797
Size: 354 126 36 900 381
Fragments arranged by size:
900 381 354 126 36
BstYI r'GATC_y
Cuts at: 0 587 1775 1797
Size: 587 1188 22
Fragments arranged by size:
1188 587 22
BtgI C'CryG_G
Cuts at: 0 635 833 965 1797
Size: 635 198 132 832
Fragments arranged by size:
832 635 198 132
BtsI GCAGTG_nn'
Cuts at: 0 786 1162 1396 1797
Size: 786 376 234 401
Fragments arranged by size:
786 401 376 234
EaeI y'GGCC_r
Cuts at: 0 185 206 311 962 983 1053 1130 1190
Size: 185 21 105 651 21 70 77 60
Cuts at: 1190 1298 1364 1418 1541 1612 1797
Size: 108 66 54 123 71 185
Fragments arranged by size:
651 185 185 123 108 105 77 71
70 66 60 54 21 21
EagI C'GGCC_G
Cuts at: 0 1053 1190 1612 1797
Size: 1053 137 422 185
Fragments arranged by size:
1053 422 185 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 273 925 1621 1797
Size: 273 652 696 176
Fragments arranged by size:
696 652 273 176
EcoICRI GAG'CTC
Cuts at: 0 177 951 1797
Size: 177 774 846
Fragments arranged by size:
846 774 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 86 169 646 667 1696 1797
Size: 75 11 83 477 21 1029 101
Fragments arranged by size:
1029 477 101 83 75 21 11
EcoO109I rG'GnC_Cy
Cuts at: 0 27 86 1555 1797
Size: 27 59 1469 242
Fragments arranged by size:
1469 242 59 27
FseI GG_CCGG'CC
Cuts at: 0 1618 1797
Size: 1618 179
HaeII r_GCGC'y
Cuts at: 0 635 706 872 1148 1328 1611 1797
Size: 635 71 166 276 180 283 186
Fragments arranged by size:
635 283 276 186 180 166 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 1797
Size: 126 32 1639
Fragments arranged by size:
1639 126 32
HincII GTy'rAC
Cuts at: 0 54 489 1797
Size: 54 435 1308
Fragments arranged by size:
1308 435 54
KasI G'GCGC_C
Cuts at: 0 702 868 1144 1607 1797
Size: 702 166 276 463 190
Fragments arranged by size:
702 463 276 190 166
KpnI G_GTAC'C
Cuts at: 0 1386 1797
Size: 1386 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 737 788 1283 1797
Size: 371 366 51 495 514
Fragments arranged by size:
514 495 371 366 51
MscI TGG'CCA
Cuts at: 0 1300 1420 1797
Size: 1300 120 377
Fragments arranged by size:
1300 377 120
MslI CAynn'nnrTG
Cuts at: 0 373 478 772 1797
Size: 373 105 294 1025
Fragments arranged by size:
1025 373 294 105
MspA1I CmG'CkG
Cuts at: 0 147 294 569 863 1107 1416 1454 1546
Size: 147 147 275 294 244 309 38 92
Cuts at: 1546 1605 1703 1797
Size: 59 98 94
Fragments arranged by size:
309 294 275 244 147 147 98 94
92 59 38
NaeI GCC'GGC
Cuts at: 0 189 210 987 1149 1368 1612 1616 1797
Size: 189 21 777 162 219 244 4 181
Fragments arranged by size:
777 244 219 189 181 162 21 4
NarI GG'CG_CC
Cuts at: 0 703 869 1145 1608 1797
Size: 703 166 276 463 189
Fragments arranged by size:
703 463 276 189 166
NgoMIV G'CCGG_C
Cuts at: 0 187 208 985 1147 1366 1610 1614 1797
Size: 187 21 777 162 219 244 4 183
Fragments arranged by size:
777 244 219 187 183 162 21 4
NheI G'CTAG_C
Cuts at: 0 663 1797
Size: 663 1134
NotI GC'GGCC_GC
Cuts at: 0 1053 1797
Size: 1053 744
PasI CC'CwG_GG
Cuts at: 0 669 692 1682 1797
Size: 669 23 990 115
Fragments arranged by size:
990 669 115 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1674 1797
Size: 297 1377 123
Fragments arranged by size:
1377 297 123
PpuMI rG'GwC_Cy
Cuts at: 0 27 86 1555 1797
Size: 27 59 1469 242
Fragments arranged by size:
1469 242 59 27
PstI C_TGCA'G
Cuts at: 0 1169 1334 1604 1726 1797
Size: 1169 165 270 122 71
Fragments arranged by size:
1169 270 165 122 71
PvuII CAG'CTG
Cuts at: 0 147 294 863 1797
Size: 147 147 569 934
Fragments arranged by size:
934 569 147 147
SacI G_AGCT'C
Cuts at: 0 179 953 1797
Size: 179 774 844
Fragments arranged by size:
844 774 179
SanDI GG'GwC_CC
Cuts at: 0 1555 1797
Size: 1555 242
SbfI CC_TGCA'GG
Cuts at: 0 1334 1797
Size: 1334 463
SexAI A'CCwGG_T
Cuts at: 0 1385 1797
Size: 1385 412
SfcI C'TryA_G
Cuts at: 0 1165 1330 1600 1722 1797
Size: 1165 165 270 122 75
Fragments arranged by size:
1165 270 165 122 75
SfoI GGC'GCC
Cuts at: 0 704 870 1146 1609 1797
Size: 704 166 276 463 188
Fragments arranged by size:
704 463 276 188 166
SmlI C'TyrA_G
Cuts at: 0 220 715 1797
Size: 220 495 1082
Fragments arranged by size:
1082 495 220
StuI AGG'CCT
Cuts at: 0 226 1753 1797
Size: 226 1527 44
Fragments arranged by size:
1527 226 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 661 705 1446 1797
Size: 472 189 44 741 351
Fragments arranged by size:
741 472 351 189 44
TatI w'GTAC_w
Cuts at: 0 248 1797
Size: 248 1549
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 1284 1797
Size: 642 642 513
Fragments arranged by size:
642 642 513
ZraI GAC'GTC
Cuts at: 0 273 1797
Size: 273 1524
Enzymes that do cut:
AatII AccI Acc65I AcuI AloI AlwNI ApaLI ApoI
BanI BanII BbeI BbsI BbvCI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
FseI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NotI PasI
PflMI PpuMI PstI PvuII SacI SanDI SbfI SexAI
SfcI SfoI SmlI StuI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BcgI BciVI BfrBI BplI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EcoNI EcoRI EcoRV FalI FspI
FspAI HindIII HpaI MfeI MluI NcoI NdeI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI