Restriction map for
pFN21AE2442
(Linear) (Six-base) MAPSORT of: KIEE2442.seq Check: 1049 from: 1 to: 5868
KIEE2442.orf
With 182 enzymes: SgfI *
February 19, 2013 13:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2721 3408 4074 5868
Size: 2721 687 666 1794
Fragments arranged by size:
2721 1794 687 666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 2778 3743 3867 4698 5482 5868
Size: 715 2063 965 124 831 784 386
Fragments arranged by size:
2063 965 831 784 715 386 124
AflII C'TTAA_G
Cuts at: 0 3607 4678 5868
Size: 3607 1071 1190
Fragments arranged by size:
3607 1190 1071
AflIII A'CryG_T
Cuts at: 0 2558 4172 4178 4919 5144 5225 5868
Size: 2558 1614 6 741 225 81 643
Fragments arranged by size:
2558 1614 741 643 225 81 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 101 202 5868
Size: 101 101 5666
Fragments arranged by size:
5666 101 101
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 1234 1266 5868
Size: 438 32 764 32 4602
Fragments arranged by size:
4602 764 438 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 679 1095 2804 2833 3148 5566 5868
Size: 679 416 1709 29 315 2418 302
Fragments arranged by size:
2418 1709 679 416 315 302 29
ApaI G_GGCC'C
Cuts at: 0 327 714 1821 5024 5867 5868
Size: 327 387 1107 3203 843 1
Fragments arranged by size:
3203 1107 843 387 327 1
ApoI r'AATT_y
Cuts at: 0 4 4517 5868
Size: 4 4513 1351
Fragments arranged by size:
4513 1351 4
AvaI C'yCGr_G
Cuts at: 0 320 1264 1870 4136 5868
Size: 320 944 606 2266 1732
Fragments arranged by size:
2266 1732 944 606 320
AvrII C'CTAG_G
Cuts at: 0 2424 5433 5868
Size: 2424 3009 435
Fragments arranged by size:
3009 2424 435
BamHI G'GATC_C
Cuts at: 0 16 1989 2747 4775 5868
Size: 16 1973 758 2028 1093
Fragments arranged by size:
2028 1973 1093 758 16
BanI G'GyrC_C
Cuts at: 0 3826 5059 5868
Size: 3826 1233 809
Fragments arranged by size:
3826 1233 809
BanII G_rGCy'C
Cuts at: 0 209 233 327 714 1408 1606 1821 2006
Size: 209 24 94 387 694 198 215 185
Cuts at: 2006 2071 2911 2921 3142 3780 4351 5024 5083
Size: 65 840 10 221 638 571 673 59
Cuts at: 5083 5204 5867 5868
Size: 121 663 1
Fragments arranged by size:
840 694 673 663 638 571 387 221
215 209 198 185 121 94 65 59
24 10 1
BbeI G_GCGC'C
Cuts at: 0 3830 5868
Size: 3830 2038
BbsI GAAGACnn'nnnn_
Cuts at: 0 468 1421 1783 2130 2480 2517 3521 3567
Size: 468 953 362 347 350 37 1004 46
Cuts at: 3567 4545 5558 5868
Size: 978 1013 310
Fragments arranged by size:
1013 1004 978 953 468 362 350 347
310 46 37
BbvCI CC'TCA_GC
Cuts at: 0 2999 3224 4999 5645 5868
Size: 2999 225 1775 646 223
Fragments arranged by size:
2999 1775 646 225 223
BciVI GTATCCnnnnn_n'
Cuts at: 0 2542 3378 5868
Size: 2542 836 2490
Fragments arranged by size:
2542 2490 836
BclI T'GATC_A
Cuts at: 0 353 4469 5868
Size: 353 4116 1399
Fragments arranged by size:
4116 1399 353
BfrBI ATG'CAT
Cuts at: 0 3356 5868
Size: 3356 2512
BglI GCCn_nnn'nGGC
Cuts at: 0 322 1601 1637 1825 2487 5868
Size: 322 1279 36 188 662 3381
Fragments arranged by size:
3381 1279 662 322 188 36
BglII A'GATC_T
Cuts at: 0 618 5868
Size: 618 5250
BlpI GC'TnA_GC
Cuts at: 0 3490 5868
Size: 3490 2378
Bme1580I G_kGCm'C
Cuts at: 0 327 714 1821 1894 2358 2993 5024 5639
Size: 327 387 1107 73 464 635 2031 615
Cuts at: 5639 5867 5868
Size: 228 1
Fragments arranged by size:
2031 1107 635 615 464 387 327 228
73 1
BmgBI CAC'GTC
Cuts at: 0 3191 4976 5868
Size: 3191 1785 892
Fragments arranged by size:
3191 1785 892
BmrI ACTGGGnnnn_n'
Cuts at: 0 2368 5868
Size: 2368 3500
BmtI G_CTAG'C
Cuts at: 0 1297 5868
Size: 1297 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1601 1668 1757 2074 2844 2933 3577
Size: 256 1345 67 89 317 770 89 644
Cuts at: 3577 5185 5402 5868
Size: 1608 217 466
Fragments arranged by size:
1608 1345 770 644 466 317 256 217
89 89 67
Bpu10I CC'TnA_GC
Cuts at: 0 2834 2999 3224 3305 4999 5645 5868
Size: 2834 165 225 81 1694 646 223
Fragments arranged by size:
2834 1694 646 225 223 165 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1882 1945 5868
Size: 1882 63 3923
Fragments arranged by size:
3923 1882 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 1577 2988 5230 5868
Size: 1577 1411 2242 638
Fragments arranged by size:
2242 1577 1411 638
BsaAI yAC'GTr
Cuts at: 0 548 5868
Size: 548 5320
BsaBI GATnn'nnATC
Cuts at: 0 245 5868
Size: 245 5623
BsaHI Gr'CG_yC
Cuts at: 0 3827 5868
Size: 3827 2041
BsaWI w'CCGG_w
Cuts at: 0 51 266 1274 1520 5868
Size: 51 215 1008 246 4348
Fragments arranged by size:
4348 1008 246 215 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1234 1264 1666 1696 3229 3259 5467 5497
Size: 1234 30 402 30 1533 30 2208 30
Cuts at: 5497 5868
Size: 371
Fragments arranged by size:
2208 1533 1234 402 371 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1216 1888 3237 3844 4324 4987 5203 5491
Size: 1216 672 1349 607 480 663 216 288
Cuts at: 5491 5662 5665 5868
Size: 171 3 203
Fragments arranged by size:
1349 1216 672 663 607 480 288 216
203 171 3
BseYI C'CCAG_C
Cuts at: 0 158 210 1614 1669 2229 2816 4985 5082
Size: 158 52 1404 55 560 587 2169 97
Cuts at: 5082 5868
Size: 786
Fragments arranged by size:
2169 1404 786 587 560 158 97 55
52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5584 5868
Size: 5584 284
BsiEI CG_ry'CG
Cuts at: 0 4982 5868
Size: 4982 886
BsiHKAI G_wGCw'C
Cuts at: 0 209 1136 2006 2057 2911 2951 3452 5197
Size: 209 927 870 51 854 40 501 1745
Cuts at: 5197 5868
Size: 671
Fragments arranged by size:
1745 927 870 854 671 501 209 51
40
BsmI GAATG_Cn'
Cuts at: 0 1756 3356 5686 5868
Size: 1756 1600 2330 182
Fragments arranged by size:
2330 1756 1600 182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3181 5868
Size: 3181 2687
Bsp1286I G_dGCh'C
Cuts at: 0 209 233 327 714 1136 1408 1606 1821
Size: 209 24 94 387 422 272 198 215
Cuts at: 1821 1894 2006 2057 2071 2358 2911 2921 2951
Size: 73 112 51 14 287 553 10 30
Cuts at: 2951 2993 3142 3452 3780 4351 5024 5083 5197
Size: 42 149 310 328 571 673 59 114
Cuts at: 5197 5204 5639 5867 5868
Size: 7 435 228 1
Fragments arranged by size:
673 571 553 435 422 387 328 310
287 272 228 215 209 198 149 114
112 94 73 59 51 42 30 24
14 10 7 1
BspEI T'CCGG_A
Cuts at: 0 51 1274 5868
Size: 51 1223 4594
Fragments arranged by size:
4594 1223 51
BspHI T'CATG_A
Cuts at: 0 1055 3479 3842 4301 4571 4799 5868
Size: 1055 2424 363 459 270 228 1069
Fragments arranged by size:
2424 1069 1055 459 363 270 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2721 2735 2789 3408 4074 5375 5868
Size: 2721 14 54 619 666 1301 493
Fragments arranged by size:
2721 1301 666 619 493 54 14
BsrBI CCG'CTC
Cuts at: 0 1433 4765 5606 5868
Size: 1433 3332 841 262
Fragments arranged by size:
3332 1433 841 262
BsrDI GCAATG_nn'
Cuts at: 0 939 1835 2147 2882 2993 4067 4649 5061
Size: 939 896 312 735 111 1074 582 412
Cuts at: 5061 5150 5868
Size: 89 718
Fragments arranged by size:
1074 939 896 735 718 582 412 312
111 89
BsrFI r'CCGG_y
Cuts at: 0 4981 5868
Size: 4981 887
BsrGI T'GTAC_A
Cuts at: 0 4175 5147 5868
Size: 4175 972 721
Fragments arranged by size:
4175 972 721
BssSI C'ACGA_G
Cuts at: 0 387 5868
Size: 387 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1290 1762 2804 3431 4955 5868
Size: 1290 472 1042 627 1524 913
Fragments arranged by size:
1524 1290 1042 913 627 472
BstBI TT'CG_AA
Cuts at: 0 2078 3747 5868
Size: 2078 1669 2121
Fragments arranged by size:
2121 2078 1669
BstEII G'GTnAC_C
Cuts at: 0 4066 5868
Size: 4066 1802
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2043 2379 2758 4482 5844 5868
Size: 2043 336 379 1724 1362 24
Fragments arranged by size:
2043 1724 1362 379 336 24
BstYI r'GATC_y
Cuts at: 0 16 240 246 558 618 629 937 1150
Size: 16 224 6 312 60 11 308 213
Cuts at: 1150 1989 2747 3276 3299 3499 4775 5390 5868
Size: 839 758 529 23 200 1276 615 478
Fragments arranged by size:
1276 839 758 615 529 478 312 308
224 213 200 60 23 16 11 6
Bsu36I CC'TnA_GG
Cuts at: 0 1096 1550 3212 3281 3292 5868
Size: 1096 454 1662 69 11 2576
Fragments arranged by size:
2576 1662 1096 454 69 11
BtgI C'CryG_G
Cuts at: 0 624 1190 2048 2058 3400 3539 4375 5183
Size: 624 566 858 10 1342 139 836 808
Cuts at: 5183 5609 5868
Size: 426 259
Fragments arranged by size:
1342 858 836 808 624 566 426 259
139 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2640 2946 5868
Size: 2640 306 2922
Fragments arranged by size:
2922 2640 306
BtsI GCAGTG_nn'
Cuts at: 0 951 1512 2894 3501 3769 4783 5162 5394
Size: 951 561 1382 607 268 1014 379 232
Cuts at: 5394 5868
Size: 474
Fragments arranged by size:
1382 1014 951 607 561 474 379 268
232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1883 1918 4141 4176 5868
Size: 1883 35 2223 35 1692
Fragments arranged by size:
2223 1883 1692 35 35
DraI TTT'AAA
Cuts at: 0 4098 5868
Size: 4098 1770
DraIII CAC_nnn'GTG
Cuts at: 0 726 2018 5868
Size: 726 1292 3850
Fragments arranged by size:
3850 1292 726
DrdI GACnn_nn'nnGTC
Cuts at: 0 844 1785 1911 5868
Size: 844 941 126 3957
Fragments arranged by size:
3957 941 844 126
EaeI y'GGCC_r
Cuts at: 0 365 1530 1629 2045 2237 2297 2381 2580
Size: 365 1165 99 416 192 60 84 199
Cuts at: 2580 2760 3766 4547 4740 4766 4979 5180 5868
Size: 180 1006 781 193 26 213 201 688
Fragments arranged by size:
1165 1006 781 688 416 365 213 201
199 193 192 180 99 84 60 26
EagI C'GGCC_G
Cuts at: 0 4979 5868
Size: 4979 889
EarI CTCTTCn'nnn_
Cuts at: 0 137 805 1664 3910 4717 4825 4966 5868
Size: 137 668 859 2246 807 108 141 902
Fragments arranged by size:
2246 902 859 807 668 141 137 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1039 4714 5868
Size: 1039 3675 1154
Fragments arranged by size:
3675 1154 1039
EcoICRI GAG'CTC
Cuts at: 0 207 2004 2909 5868
Size: 207 1797 905 2959
Fragments arranged by size:
2959 1797 905 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 715 1601 1668 1757 2074 2778 2844
Size: 256 459 886 67 89 317 704 66
Cuts at: 2844 2933 3577 3743 3867 4698 5185 5402 5482
Size: 89 644 166 124 831 487 217 80
Cuts at: 5482 5868
Size: 386
Fragments arranged by size:
886 831 704 644 487 459 386 317
256 217 166 124 89 89 80 67
66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5209 5868
Size: 5209 659
EcoO109I rG'GnC_Cy
Cuts at: 0 324 710 711 1260 1639 2801 2935 3165
Size: 324 386 1 549 379 1162 134 230
Cuts at: 3165 3216 5020 5021 5868
Size: 51 1804 1 847
Fragments arranged by size:
1804 1162 847 549 386 379 324 230
134 51 1 1
EcoRI G'AATT_C
Cuts at: 0 4 5868
Size: 4 5864
EcoRV GAT'ATC
Cuts at: 0 888 1539 5868
Size: 888 651 4329
Fragments arranged by size:
4329 888 651
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1800 5868
Size: 1800 4068
FspI TGC'GCA
Cuts at: 0 3424 5868
Size: 3424 2444
HaeII r_GCGC'y
Cuts at: 0 3830 5868
Size: 3830 2038
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1303 1335 1408 1440 2992 3024 3229 3261
Size: 1303 32 73 32 1552 32 205 32
Cuts at: 3261 5296 5328 5375 5407 5644 5676 5868
Size: 2035 32 47 32 237 32 192
Fragments arranged by size:
2035 1552 1303 237 205 192 73 47
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1167 4044 4146 5142 5796 5868
Size: 1167 2877 102 996 654 72
Fragments arranged by size:
2877 1167 996 654 102 72
HindIII A'AGCT_T
Cuts at: 0 3548 4427 5868
Size: 3548 879 1441
Fragments arranged by size:
3548 1441 879
KasI G'GCGC_C
Cuts at: 0 3826 5868
Size: 3826 2042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2995 5432 5868
Size: 2995 2437 436
Fragments arranged by size:
2995 2437 436
MscI TGG'CCA
Cuts at: 0 1631 2047 2239 2299 2383 2582 2762 5182
Size: 1631 416 192 60 84 199 180 2420
Cuts at: 5182 5868
Size: 686
Fragments arranged by size:
2420 1631 686 416 199 192 180 84
60
MslI CAynn'nnrTG
Cuts at: 0 373 1505 1951 2026 2041 2047 3478 3802
Size: 373 1132 446 75 15 6 1431 324
Cuts at: 3802 3895 4300 4444 4477 4480 4576 4636 5155
Size: 93 405 144 33 3 96 60 519
Cuts at: 5155 5842 5868
Size: 687 26
Fragments arranged by size:
1431 1132 687 519 446 405 373 324
144 96 93 75 60 33 26 15
6 3
MspA1I CmG'CkG
Cuts at: 0 162 186 2486 3096 3455 4126 5868
Size: 162 24 2300 610 359 671 1742
Fragments arranged by size:
2300 1742 671 610 359 162 24
NaeI GCC'GGC
Cuts at: 0 4983 5868
Size: 4983 885
NarI GG'CG_CC
Cuts at: 0 3827 5868
Size: 3827 2041
NcoI C'CATG_G
Cuts at: 0 1190 2048 2058 3400 5609 5868
Size: 1190 858 10 1342 2209 259
Fragments arranged by size:
2209 1342 1190 858 259 10
NdeI CA'TA_TG
Cuts at: 0 600 2593 5868
Size: 600 1993 3275
Fragments arranged by size:
3275 1993 600
NgoMIV G'CCGG_C
Cuts at: 0 4981 5868
Size: 4981 887
NheI G'CTAG_C
Cuts at: 0 1293 5868
Size: 1293 4575
NsiI A_TGCA'T
Cuts at: 0 3358 5868
Size: 3358 2510
NspI r_CATG'y
Cuts at: 0 2496 2562 3801 4152 4182 4923 4953 5148
Size: 2496 66 1239 351 30 741 30 195
Cuts at: 5148 5229 5868
Size: 81 639
Fragments arranged by size:
2496 1239 741 639 351 195 81 66
30 30
PasI CC'CwG_GG
Cuts at: 0 2939 3180 5860 5868
Size: 2939 241 2680 8
Fragments arranged by size:
2939 2680 241 8
PciI A'CATG_T
Cuts at: 0 2558 4178 4919 5144 5225 5868
Size: 2558 1620 741 225 81 643
Fragments arranged by size:
2558 1620 741 643 225 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 2306 3148 3650 5400 5868
Size: 2306 842 502 1750 468
Fragments arranged by size:
2306 1750 842 502 468
PfoI T'CCnGG_A
Cuts at: 0 1253 1578 5868
Size: 1253 325 4290
Fragments arranged by size:
4290 1253 325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 5868
Size: 1234 32 4602
Fragments arranged by size:
4602 1234 32
PpuMI rG'GwC_Cy
Cuts at: 0 2801 3165 3216 5868
Size: 2801 364 51 2652
Fragments arranged by size:
2801 2652 364 51
PspOMI G'GGCC_C
Cuts at: 0 323 710 1817 5020 5863 5868
Size: 323 387 1107 3203 843 5
Fragments arranged by size:
3203 1107 843 387 323 5
PspXI vC'TCGA_Gb
Cuts at: 0 1870 5868
Size: 1870 3998
PstI C_TGCA'G
Cuts at: 0 2732 3101 4085 5868
Size: 2732 369 984 1783
Fragments arranged by size:
2732 1783 984 369
PvuII CAG'CTG
Cuts at: 0 162 186 3096 3455 4126 5868
Size: 162 24 2910 359 671 1742
Fragments arranged by size:
2910 1742 671 359 162 24
SacI G_AGCT'C
Cuts at: 0 209 2006 2911 5868
Size: 209 1797 905 2957
Fragments arranged by size:
2957 1797 905 209
SapI GCTCTTCn'nnn_
Cuts at: 0 4717 5868
Size: 4717 1151
SbfI CC_TGCA'GG
Cuts at: 0 2732 5868
Size: 2732 3136
ScaI AGT'ACT
Cuts at: 0 3637 4324 4597 5868
Size: 3637 687 273 1271
Fragments arranged by size:
3637 1271 687 273
SexAI A'CCwGG_T
Cuts at: 0 1160 2633 5868
Size: 1160 1473 3235
Fragments arranged by size:
3235 1473 1160
SfcI C'TryA_G
Cuts at: 0 255 1181 2728 3097 4081 5868
Size: 255 926 1547 369 984 1787
Fragments arranged by size:
1787 1547 984 926 369 255
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1637 5868
Size: 1637 4231
SfoI GGC'GCC
Cuts at: 0 3828 5868
Size: 3828 2040
SmaI CCC'GGG
Cuts at: 0 4138 5868
Size: 4138 1730
SmlI C'TyrA_G
Cuts at: 0 1264 1861 1870 1960 3607 4678 5868
Size: 1264 597 9 90 1647 1071 1190
Fragments arranged by size:
1647 1264 1190 1071 597 90 9
SpeI A'CTAG_T
Cuts at: 0 1720 5868
Size: 1720 4148
SphI G_CATG'C
Cuts at: 0 3801 4953 5868
Size: 3801 1152 915
Fragments arranged by size:
3801 1152 915
SspI AAT'ATT
Cuts at: 0 3718 5868
Size: 3718 2150
StuI AGG'CCT
Cuts at: 0 638 692 1472 5581 5780 5868
Size: 638 54 780 4109 199 88
Fragments arranged by size:
4109 780 638 199 88 54
StyI C'CwwG_G
Cuts at: 0 562 782 1190 2048 2058 2424 2822 2867
Size: 562 220 408 858 10 366 398 45
Cuts at: 2867 3400 4769 5433 5609 5868
Size: 533 1369 664 176 259
Fragments arranged by size:
1369 858 664 562 533 408 398 366
259 220 176 45 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1606 1941 3147 3172 3603 3883 5868
Size: 1606 335 1206 25 431 280 1985
Fragments arranged by size:
1985 1606 1206 431 335 280 25
TatI w'GTAC_w
Cuts at: 0 3560 3635 4175 4187 4322 4595 5147 5709
Size: 3560 75 540 12 135 273 552 562
Cuts at: 5709 5868
Size: 159
Fragments arranged by size:
3560 562 552 540 273 159 135 75
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2333 2365 3381 3413 5868
Size: 2333 32 1016 32 2455
Fragments arranged by size:
2455 2333 1016 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3571 5868
Size: 3571 2297
XbaI T'CTAG_A
Cuts at: 0 243 5868
Size: 243 5625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 958 1333 4341 5868
Size: 958 375 3008 1527
Fragments arranged by size:
3008 1527 958 375
XhoI C'TCGA_G
Cuts at: 0 1264 1870 5868
Size: 1264 606 3998
Fragments arranged by size:
3998 1264 606
XmaI C'CCGG_G
Cuts at: 0 4136 5868
Size: 4136 1732
XmnI GAAnn'nnTTC
Cuts at: 0 1249 1780 2674 3156 3904 4390 4579 5868
Size: 1249 531 894 482 748 486 189 1289
Fragments arranged by size:
1289 1249 894 748 531 486 482 189
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AloI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII
SacI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
ApaLI AscI AseI AsiSI BaeI BcgI BplI BsiWI
BssHII BstZ17I ClaI FseI FspAI HpaI KpnI MfeI
MluI NotI NruI PacI PmeI PmlI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SgfI SgrAI
SnaBI SrfI SwaI ZraI