Restriction map for
pFN21AE2440
(Linear) (Six-base) MAPSORT of: KIEE2440.seq Check: 2639 from: 1 to: 9225
KIEE2440.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2422 4270 5348 8023 9138 9225
Size: 2422 1848 1078 2675 1115 87
Fragments arranged by size:
2675 2422 1848 1115 1078 87
AatII G_ACGT'C
Cuts at: 0 1829 3292 7903 9225
Size: 1829 1463 4611 1322
Fragments arranged by size:
4611 1829 1463 1322
AccI GT'mk_AC
Cuts at: 0 1799 3151 5594 5603 8003 8438 9019 9225
Size: 1799 1352 2443 9 2400 435 581 206
Fragments arranged by size:
2443 2400 1799 1352 581 435 206 9
Acc65I G'GTAC_C
Cuts at: 0 920 4136 7726 9225
Size: 920 3216 3590 1499
Fragments arranged by size:
3590 3216 1499 920
AclI AA'CG_TT
Cuts at: 0 1427 9225
Size: 1427 7798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2632 3730 5059 5195 6023 9150 9225
Size: 2632 1098 1329 136 828 3127 75
Fragments arranged by size:
3127 2632 1329 1098 828 136 75
AfeI AGC'GCT
Cuts at: 0 621 9225
Size: 621 8604
AflII C'TTAA_G
Cuts at: 0 336 9225
Size: 336 8889
AflIII A'CryG_T
Cuts at: 0 967 5391 5573 7620 7668 9225
Size: 967 4424 182 2047 48 1557
Fragments arranged by size:
4424 2047 1557 967 182 48
AleI CACnn'nnGTG
Cuts at: 0 3635 9225
Size: 3635 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 470 3653 5165 5356 8086 9225
Size: 470 3183 1512 191 2730 1139
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8488 8520 9225
Size: 8488 32 705
Fragments arranged by size:
8488 705 32
AlwNI CAG_nnn'CTG
Cuts at: 0 443 911 1686 2524 3031 5476 5733 6105
Size: 443 468 775 838 507 2445 257 372
Cuts at: 6105 6168 9225
Size: 63 3057
Fragments arranged by size:
3057 2445 838 775 507 468 443 372
257 63
ApaI G_GGCC'C
Cuts at: 0 1409 9225
Size: 1409 7816
ApoI r'AATT_y
Cuts at: 0 649 2210 3202 3873 4766 5192 5371 6224
Size: 649 1561 992 671 893 426 179 853
Cuts at: 6224 7416 7880 9225
Size: 1192 464 1345
Fragments arranged by size:
1561 1345 1192 992 893 853 671 649
464 426 179
AvaI C'yCGr_G
Cuts at: 0 1490 1714 2342 2460 4602 8081 9225
Size: 1490 224 628 118 2142 3479 1144
Fragments arranged by size:
3479 2142 1490 1144 628 224 118
AvrII C'CTAG_G
Cuts at: 0 8511 9225
Size: 8511 714
BamHI G'GATC_C
Cuts at: 0 747 9225
Size: 747 8478
BanI G'GyrC_C
Cuts at: 0 262 427 567 920 1643 2635 3119 3403
Size: 262 165 140 353 723 992 484 284
Cuts at: 3403 3748 4136 5546 7726 9225
Size: 345 388 1410 2180 1499
Fragments arranged by size:
2180 1499 1410 992 723 484 388 353
345 284 262 165 140
BanII G_rGCy'C
Cuts at: 0 1397 1409 2759 3520 4610 5426 6550 6664
Size: 1397 12 1350 761 1090 816 1124 114
Cuts at: 6664 7454 8081 8897 9145 9225
Size: 790 627 816 248 80
Fragments arranged by size:
1397 1350 1124 1090 816 816 790 761
627 248 114 80 12
BbeI G_GCGC'C
Cuts at: 0 1647 2639 9225
Size: 1647 992 6586
Fragments arranged by size:
6586 1647 992
BbsI GAAGACnn'nnnn_
Cuts at: 0 6917 9225
Size: 6917 2308
BbvCI CC'TCA_GC
Cuts at: 0 1868 3940 5291 5312 5612 5872 6076 9225
Size: 1868 2072 1351 21 300 260 204 3149
Fragments arranged by size:
3149 2072 1868 1351 300 260 204 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1945 1979 9159 9193 9225
Size: 1945 34 7180 34 32
Fragments arranged by size:
7180 1945 34 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 6619 9225
Size: 6619 2606
BclI T'GATC_A
Cuts at: 0 1598 2009 6314 9225
Size: 1598 411 4305 2911
Fragments arranged by size:
4305 2911 1598 411
BglI GCCn_nnn'nGGC
Cuts at: 0 174 939 1328 1717 2174 9225
Size: 174 765 389 389 457 7051
Fragments arranged by size:
7051 765 457 389 389 174
BglII A'GATC_T
Cuts at: 0 708 9225
Size: 708 8517
BlpI GC'TnA_GC
Cuts at: 0 4332 6094 8221 9225
Size: 4332 1762 2127 1004
Fragments arranged by size:
4332 2127 1762 1004
Bme1580I G_kGCm'C
Cuts at: 0 1282 1409 2460 2952 3095 3406 3568 7628
Size: 1282 127 1051 492 143 311 162 4060
Cuts at: 7628 7831 9225
Size: 203 1394
Fragments arranged by size:
4060 1394 1282 1051 492 311 203 162
143 127
BmgBI CAC'GTC
Cuts at: 0 5256 6047 6084 6219 9225
Size: 5256 791 37 135 3006
Fragments arranged by size:
5256 3006 791 135 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 1366 1713 3105 3499 8523 9009 9225
Size: 324 1042 347 1392 394 5024 486 216
Fragments arranged by size:
5024 1392 1042 486 394 347 324 216
BmtI G_CTAG'C
Cuts at: 0 7204 9130 9225
Size: 7204 1926 95
Fragments arranged by size:
7204 1926 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1706 2079 3703 4168 7721 9225
Size: 1706 373 1624 465 3553 1504
Fragments arranged by size:
3553 1706 1624 1504 465 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 3053 3331 3505 4786 5829 7468 8140
Size: 957 2096 278 174 1281 1043 1639 672
Cuts at: 8140 8226 8281 8773 9225
Size: 86 55 492 452
Fragments arranged by size:
2096 1639 1281 1043 957 672 492 452
278 174 86 55
Bpu10I CC'TnA_GC
Cuts at: 0 721 1868 3940 5291 5312 5612 5872 6076
Size: 721 1147 2072 1351 21 300 260 204
Cuts at: 6076 8036 9225
Size: 1960 1189
Fragments arranged by size:
2072 1960 1351 1189 1147 721 300 260
204 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 2767 3579 3752 4461 5149 9225
Size: 595 2172 812 173 709 688 4076
Fragments arranged by size:
4076 2172 812 709 688 595 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 2743 3325 3674 8155 8361 9225
Size: 2743 582 349 4481 206 864
Fragments arranged by size:
4481 2743 864 582 349 206
BsaAI yAC'GTr
Cuts at: 0 3495 7623 9225
Size: 3495 4128 1602
Fragments arranged by size:
4128 3495 1602
BsaBI GATnn'nnATC
Cuts at: 0 4999 9225
Size: 4999 4226
BsaHI Gr'CG_yC
Cuts at: 0 1644 1826 2297 2636 3289 3299 3743 4430
Size: 1644 182 471 339 653 10 444 687
Cuts at: 4430 7900 8780 9225
Size: 3470 880 445
Fragments arranged by size:
3470 1644 880 687 653 471 445 444
339 182 10
BsaWI w'CCGG_w
Cuts at: 0 1785 4610 7184 7457 9225
Size: 1785 2825 2574 273 1768
Fragments arranged by size:
2825 2574 1785 1768 273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1061 1091 2423 2453 3026 3056 3103 3133
Size: 1061 30 1332 30 573 30 47 30
Cuts at: 3133 6532 6562 8488 8518 9225
Size: 3399 30 1926 30 707
Fragments arranged by size:
3399 1926 1332 1061 707 573 47 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1082 1085 4464 6289 8757 9225
Size: 1082 3 3379 1825 2468 468
Fragments arranged by size:
3379 2468 1825 1082 468 3
BseYI C'CCAG_C
Cuts at: 0 286 3513 4155 4593 9225
Size: 286 3227 642 438 4632
Fragments arranged by size:
4632 3227 642 438 286
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 644 1383 2513 2871 3659 4030 5447
Size: 528 116 739 1130 358 788 371 1417
Cuts at: 5447 5723 5854 7696 8003 8287 8351 9169 9172
Size: 276 131 1842 307 284 64 818 3
Cuts at: 9172 9225
Size: 53
Fragments arranged by size:
1842 1417 1130 818 788 739 528 371
358 307 284 276 131 116 64 53
3
BsiEI CG_ry'CG
Cuts at: 0 3572 5831 8445 9225
Size: 3572 2259 2614 780
Fragments arranged by size:
3572 2614 2259 780
BsiHKAI G_wGCw'C
Cuts at: 0 17 2123 4350 4474 5426 5498 6073 6664
Size: 17 2106 2227 124 952 72 575 591
Cuts at: 6664 8297 8915 9225
Size: 1633 618 310
Fragments arranged by size:
2227 2106 1633 952 618 591 575 310
124 72 17
BsmI GAATG_Cn'
Cuts at: 0 290 2233 2713 5697 5949 7749 9225
Size: 290 1943 480 2984 252 1800 1476
Fragments arranged by size:
2984 1943 1800 1476 480 290 252
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1625 1786 2067 5779 9225
Size: 1625 161 281 3712 3446
Fragments arranged by size:
3712 3446 1625 281 161
Bsp1286I G_dGCh'C
Cuts at: 0 17 1282 1397 1409 2123 2460 2759 2952
Size: 17 1265 115 12 714 337 299 193
Cuts at: 2952 3095 3406 3520 3568 4350 4474 4610 5426
Size: 143 311 114 48 782 124 136 816
Cuts at: 5426 5498 6073 6550 6664 7454 7628 7831 8081
Size: 72 575 477 114 790 174 203 250
Cuts at: 8081 8297 8897 8915 9145 9225
Size: 216 600 18 230 80
Fragments arranged by size:
1265 816 790 782 714 600 575 477
337 311 299 250 230 216 203 193
174 143 136 124 115 114 114 80
72 48 18 17 12
BspEI T'CCGG_A
Cuts at: 0 7457 9225
Size: 7457 1768
BspHI T'CATG_A
Cuts at: 0 713 9225
Size: 713 8512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2422 3025 4270 4892 5348 7738 8023 9138
Size: 2422 603 1245 622 456 2390 285 1115
Cuts at: 9138 9225
Size: 87
Fragments arranged by size:
2422 2390 1245 1115 622 603 456 285
87
BsrDI GCAATG_nn'
Cuts at: 0 99 1879 2270 3183 3869 9225
Size: 99 1780 391 913 686 5356
Fragments arranged by size:
5356 1780 913 686 391 99
BsrFI r'CCGG_y
Cuts at: 0 50 2293 2843 3178 9225
Size: 50 2243 550 335 6047
Fragments arranged by size:
6047 2243 550 335 50
BsrGI T'GTAC_A
Cuts at: 0 2983 5478 5654 9225
Size: 2983 2495 176 3571
Fragments arranged by size:
3571 2983 2495 176
BssSI C'ACGA_G
Cuts at: 0 3142 5299 9225
Size: 3142 2157 3926
Fragments arranged by size:
3926 3142 2157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1088 2316 3446 7197 9225
Size: 1088 1228 1130 3751 2028
Fragments arranged by size:
3751 2028 1228 1130 1088
BstBI TT'CG_AA
Cuts at: 0 8760 9179 9225
Size: 8760 419 46
Fragments arranged by size:
8760 419 46
BstEII G'GTnAC_C
Cuts at: 0 1587 3174 4282 9225
Size: 1587 1587 1108 4943
Fragments arranged by size:
4943 1587 1587 1108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 645 2097 6039 6195 6564 7761 9225
Size: 645 1452 3942 156 369 1197 1464
Fragments arranged by size:
3942 1464 1452 1197 645 369 156
BstYI r'GATC_y
Cuts at: 0 200 708 747 1613 9225
Size: 200 508 39 866 7612
Fragments arranged by size:
7612 866 508 200 39
Bsu36I CC'TnA_GG
Cuts at: 0 3280 9225
Size: 3280 5945
BtgI C'CryG_G
Cuts at: 0 838 1103 1670 2232 2835 2952 4551 6182
Size: 838 265 567 562 603 117 1599 1631
Cuts at: 6182 6882 7721 7929 8456 9108 9225
Size: 700 839 208 527 652 117
Fragments arranged by size:
1631 1599 839 838 700 652 603 567
562 527 265 208 117 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3182 4631 9225
Size: 3182 1449 4594
Fragments arranged by size:
4594 3182 1449
BtsI GCAGTG_nn'
Cuts at: 0 52 871 1386 2317 2797 4585 6509 7563
Size: 52 819 515 931 480 1788 1924 1054
Cuts at: 7563 7740 7903 8368 8666 8798 9225
Size: 177 163 465 298 132 427
Fragments arranged by size:
1924 1788 1054 931 819 515 480 465
427 298 177 163 132 52
ClaI AT'CG_AT
Cuts at: 0 7211 9225
Size: 7211 2014
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6864 6899 9225
Size: 6864 35 2326
Fragments arranged by size:
6864 2326 35
DraI TTT'AAA
Cuts at: 0 5030 6804 9225
Size: 5030 1774 2421
Fragments arranged by size:
5030 2421 1774
DraIII CAC_nnn'GTG
Cuts at: 0 1155 2992 3064 4507 6646 9225
Size: 1155 1837 72 1443 2139 2579
Fragments arranged by size:
2579 2139 1837 1443 1155 72
DrdI GACnn_nn'nnGTC
Cuts at: 0 8842 9225
Size: 8842 383
EaeI y'GGCC_r
Cuts at: 0 819 2105 2235 3180 3210 4970 5756 5828
Size: 819 1286 130 945 30 1760 786 72
Cuts at: 5828 6041 6185 7989 8442 9225
Size: 213 144 1804 453 783
Fragments arranged by size:
1804 1760 1286 945 819 786 783 453
213 144 130 72 30
EagI C'GGCC_G
Cuts at: 0 5828 8442 9225
Size: 5828 2614 783
Fragments arranged by size:
5828 2614 783
EarI CTCTTCn'nnn_
Cuts at: 0 1889 3536 4052 5078 5433 6469 8942 9225
Size: 1889 1647 516 1026 355 1036 2473 283
Fragments arranged by size:
2473 1889 1647 1036 1026 516 355 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1766 9225
Size: 1766 7459
EcoICRI GAG'CTC
Cuts at: 0 5424 6662 9225
Size: 5424 1238 2563
Fragments arranged by size:
5424 2563 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 2632 3053 3331 3505 3730 4786 5059
Size: 957 1675 421 278 174 225 1056 273
Cuts at: 5059 5195 5829 6023 7468 8140 8226 8281 8773
Size: 136 634 194 1445 672 86 55 492
Cuts at: 8773 9150 9225
Size: 377 75
Fragments arranged by size:
1675 1445 1056 957 672 634 492 421
377 278 273 225 194 174 136 86
75 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2342 3313 3964 7288 7764 9225
Size: 2342 971 651 3324 476 1461
Fragments arranged by size:
3324 2342 1461 971 651 476
EcoO109I rG'GnC_Cy
Cuts at: 0 1208 1405 1406 2150 3347 3500 3591 4598
Size: 1208 197 1 744 1197 153 91 1007
Cuts at: 4598 4836 6540 7114 7293 9225
Size: 238 1704 574 179 1932
Fragments arranged by size:
1932 1704 1208 1197 1007 744 574 238
197 179 153 91 1
EcoRI G'AATT_C
Cuts at: 0 649 5371 7880 9225
Size: 649 4722 2509 1345
Fragments arranged by size:
4722 2509 1345 649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6253 9225
Size: 6253 2972
FspI TGC'GCA
Cuts at: 0 2282 9091 9225
Size: 2282 6809 134
Fragments arranged by size:
6809 2282 134
FspAI rTGC'GCAy
Cuts at: 0 2282 9225
Size: 2282 6943
HaeII r_GCGC'y
Cuts at: 0 623 1647 2639 5299 8138 8999 9225
Size: 623 1024 992 2660 2839 861 226
Fragments arranged by size:
2839 2660 1024 992 861 623 226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 294 326 463 495 1605 1637 1861 1893
Size: 294 32 137 32 1110 32 224 32
Cuts at: 1893 3026 3058 3144 3176 3916 3948 4051 4083
Size: 1133 32 86 32 740 32 103 32
Cuts at: 4083 4555 4587 5170 5202 6362 6394 6532 6564
Size: 472 32 583 32 1160 32 138 32
Cuts at: 6564 6651 6683 6752 6784 7010 7042 7045 7077
Size: 87 32 69 32 226 32 3 32
Cuts at: 7077 7885 7917 9225
Size: 808 32 1308
Fragments arranged by size:
1308 1160 1133 1110 808 740 583 472
294 226 224 138 137 103 87 86
69 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 3
HincII GTy'rAC
Cuts at: 0 2218 3152 5595 6138 8004 8439 8598 8859
Size: 2218 934 2443 543 1866 435 159 261
Cuts at: 8859 8979 9225
Size: 120 246
Fragments arranged by size:
2443 2218 1866 934 543 435 261 246
159 120
HindIII A'AGCT_T
Cuts at: 0 4852 5881 7356 9225
Size: 4852 1029 1475 1869
Fragments arranged by size:
4852 1869 1475 1029
HpaI GTT'AAC
Cuts at: 0 8598 9225
Size: 8598 627
KasI G'GCGC_C
Cuts at: 0 1643 2635 9225
Size: 1643 992 6590
Fragments arranged by size:
6590 1643 992
KpnI G_GTAC'C
Cuts at: 0 924 4140 7730 9225
Size: 924 3216 3590 1495
Fragments arranged by size:
3590 3216 1495 924
MfeI C'AATT_G
Cuts at: 0 3132 7792 9225
Size: 3132 4660 1433
Fragments arranged by size:
4660 3132 1433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 777 2033 2420 3769 6729 9225
Size: 626 151 1256 387 1349 2960 2496
Fragments arranged by size:
2960 2496 1349 1256 626 387 151
MscI TGG'CCA
Cuts at: 0 821 4972 5758 6043 6187 7991 9225
Size: 821 4151 786 285 144 1804 1234
Fragments arranged by size:
4151 1804 1234 821 786 285 144
MslI CAynn'nnrTG
Cuts at: 0 2237 2423 3635 3811 4015 4292 4642 4903
Size: 2237 186 1212 176 204 277 350 261
Cuts at: 4903 5147 5273 5555 5572 9225
Size: 244 126 282 17 3653
Fragments arranged by size:
3653 2237 1212 350 282 277 261 244
204 186 176 126 17
MspA1I CmG'CkG
Cuts at: 0 1672 1974 2110 2245 2555 5217 5626 8246
Size: 1672 302 136 135 310 2662 409 2620
Cuts at: 8246 8301 8610 8884 9110 9225
Size: 55 309 274 226 115
Fragments arranged by size:
2662 2620 1672 409 310 309 302 274
226 136 135 115 55
NaeI GCC'GGC
Cuts at: 0 52 2845 9225
Size: 52 2793 6380
Fragments arranged by size:
6380 2793 52
NarI GG'CG_CC
Cuts at: 0 1644 2636 9225
Size: 1644 992 6589
Fragments arranged by size:
6589 1644 992
NcoI C'CATG_G
Cuts at: 0 838 1103 2835 2952 6182 7929 8456 9225
Size: 838 265 1732 117 3230 1747 527 769
Fragments arranged by size:
3230 1747 1732 838 769 527 265 117
NdeI CA'TA_TG
Cuts at: 0 360 4761 8889 9225
Size: 360 4401 4128 336
Fragments arranged by size:
4401 4128 360 336
NgoMIV G'CCGG_C
Cuts at: 0 50 2843 9225
Size: 50 2793 6382
Fragments arranged by size:
6382 2793 50
NheI G'CTAG_C
Cuts at: 0 7200 9126 9225
Size: 7200 1926 99
Fragments arranged by size:
7200 1926 99
NspI r_CATG'y
Cuts at: 0 166 253 807 971 980 1822 5085 5395
Size: 166 87 554 164 9 842 3263 310
Cuts at: 5395 5577 7672 9225
Size: 182 2095 1553
Fragments arranged by size:
3263 2095 1553 842 554 310 182 166
164 87 9
PasI CC'CwG_GG
Cuts at: 0 3432 4132 6700 9225
Size: 3432 700 2568 2525
Fragments arranged by size:
3432 2568 2525 700
PciI A'CATG_T
Cuts at: 0 967 5391 5573 7668 9225
Size: 967 4424 182 2095 1557
Fragments arranged by size:
4424 2095 1557 967 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 272 3031 3690 3835 9225
Size: 272 2759 659 145 5390
Fragments arranged by size:
5390 2759 659 272 145
PfoI T'CCnGG_A
Cuts at: 0 4802 7257 9225
Size: 4802 2455 1968
Fragments arranged by size:
4802 2455 1968
PmlI CAC'GTG
Cuts at: 0 7623 9225
Size: 7623 1602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 8488 8520 9225
Size: 8488 32 705
Fragments arranged by size:
8488 705 32
PpuMI rG'GwC_Cy
Cuts at: 0 1208 2150 3500 4836 6540 7114 7293 9225
Size: 1208 942 1350 1336 1704 574 179 1932
Fragments arranged by size:
1932 1704 1350 1336 1208 942 574 179
PshAI GACnn'nnGTC
Cuts at: 0 1351 4433 9225
Size: 1351 3082 4792
Fragments arranged by size:
4792 3082 1351
PsiI TTA'TAA
Cuts at: 0 7145 9225
Size: 7145 2080
PspOMI G'GGCC_C
Cuts at: 0 1405 9225
Size: 1405 7820
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 8638 8670 9225
Size: 8638 32 555
Fragments arranged by size:
8638 555 32
PstI C_TGCA'G
Cuts at: 0 866 1537 2554 2860 3442 4210 4994 6173
Size: 866 671 1017 306 582 768 784 1179
Cuts at: 6173 6366 7735 9225
Size: 193 1369 1490
Fragments arranged by size:
1490 1369 1179 1017 866 784 768 671
582 306 193
PvuII CAG'CTG
Cuts at: 0 1974 2245 8301 8610 8884 9225
Size: 1974 271 6056 309 274 341
Fragments arranged by size:
6056 1974 341 309 274 271
SacI G_AGCT'C
Cuts at: 0 5426 6664 9225
Size: 5426 1238 2561
Fragments arranged by size:
5426 2561 1238
SacII CC_GC'GG
Cuts at: 0 1673 9111 9225
Size: 1673 7438 114
Fragments arranged by size:
7438 1673 114
SalI G'TCGA_C
Cuts at: 0 3150 5593 8002 8437 9225
Size: 3150 2443 2409 435 788
Fragments arranged by size:
3150 2443 2409 788 435
SanDI GG'GwC_CC
Cuts at: 0 1208 9225
Size: 1208 8017
ScaI AGT'ACT
Cuts at: 0 1699 9225
Size: 1699 7526
SexAI A'CCwGG_T
Cuts at: 0 2096 4503 5561 5597 7156 9225
Size: 2096 2407 1058 36 1559 2069
Fragments arranged by size:
2407 2096 2069 1559 1058 36
SfcI C'TryA_G
Cuts at: 0 519 862 1533 2550 2856 3438 4206 4990
Size: 519 343 671 1017 306 582 768 784
Cuts at: 4990 5604 6169 6362 7731 9225
Size: 614 565 193 1369 1494
Fragments arranged by size:
1494 1369 1017 784 768 671 614 582
565 519 343 306 193
SfoI GGC'GCC
Cuts at: 0 1645 2637 9225
Size: 1645 992 6588
Fragments arranged by size:
6588 1645 992
SgrAI Cr'CCGG_yG
Cuts at: 0 2293 9225
Size: 2293 6932
SmaI CCC'GGG
Cuts at: 0 1492 2462 8083 9225
Size: 1492 970 5621 1142
Fragments arranged by size:
5621 1492 1142 970
SmlI C'TyrA_G
Cuts at: 0 336 574 2746 3558 3767 4476 5164 9225
Size: 336 238 2172 812 209 709 688 4061
Fragments arranged by size:
4061 2172 812 709 688 336 238 209
SphI G_CATG'C
Cuts at: 0 1822 9225
Size: 1822 7403
SspI AAT'ATT
Cuts at: 0 3886 9225
Size: 3886 5339
StuI AGG'CCT
Cuts at: 0 62 1441 1720 3127 6823 6848 7694 8034
Size: 62 1379 279 1407 3696 25 846 340
Cuts at: 8034 8519 9225
Size: 485 706
Fragments arranged by size:
3696 1407 1379 846 706 485 340 279
62 25
StyI C'CwwG_G
Cuts at: 0 838 1103 1725 2835 2952 3233 3675 5433
Size: 838 265 622 1110 117 281 442 1758
Cuts at: 5433 6182 7318 7760 7929 8334 8456 8511 9225
Size: 749 1136 442 169 405 122 55 714
Fragments arranged by size:
1758 1136 1110 838 749 714 622 442
442 405 281 265 169 122 117 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 528 651 1352 3586 4064 6875 7297 7515
Size: 528 123 701 2234 478 2811 422 218
Cuts at: 7515 8575 9028 9225
Size: 1060 453 197
Fragments arranged by size:
2811 2234 1060 701 528 478 453 422
218 197 123
TatI w'GTAC_w
Cuts at: 0 1697 2983 3713 3953 4300 5478 5654 9225
Size: 1697 1286 730 240 347 1178 176 3571
Fragments arranged by size:
3571 1697 1286 1178 730 347 240 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1428 1460 2142 2174 2423 2455 2922 2954
Size: 1428 32 682 32 249 32 467 32
Cuts at: 2954 3103 3135 6862 6894 9024 9056 9225
Size: 149 32 3727 32 2130 32 169
Fragments arranged by size:
3727 2130 1428 682 467 249 169 149
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2593 5563 5599 9219 9225
Size: 2593 2970 36 3620 6
Fragments arranged by size:
3620 2970 2593 36 6
XbaI T'CTAG_A
Cuts at: 0 5490 9225
Size: 5490 3735
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2097 2425 2806 4398 7359 7523 8463 8490
Size: 2097 328 381 1592 2961 164 940 27
Cuts at: 8490 8860 9225
Size: 370 365
Fragments arranged by size:
2961 2097 1592 940 381 370 365 328
164 27
XmaI C'CCGG_G
Cuts at: 0 1490 2460 8081 9225
Size: 1490 970 5621 1144
Fragments arranged by size:
5621 1490 1144 970
ZraI GAC'GTC
Cuts at: 0 1827 3290 7901 9225
Size: 1827 1463 4611 1324
Fragments arranged by size:
4611 1827 1463 1324
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuII SacI SacII SalI SanDI
ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AgeI AhdI ApaLI AscI AseI AsiSI BaeI BfrBI
BsiWI BsrBI BssHII BstZ17I EcoRV FseI MluI NotI
NruI NsiI PacI PmeI PspXI PvuI RsrII SapI
SbfI SfiI SgfI SnaBI SpeI SrfI SwaI XhoI
XmnI