Restriction map for
pFN21AE2437
(Linear) (Six-base) MAPSORT of: KIEE2437.seq Check: 2989 from: 1 to: 1365
KIEE2437.orf
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 816 1365
Size: 816 549
AccI GT'mk_AC
Cuts at: 0 396 1365
Size: 396 969
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 183 473 1365
Size: 22 161 290 892
Fragments arranged by size:
892 290 161 22
AlwNI CAG_nnn'CTG
Cuts at: 0 451 1365
Size: 451 914
ApaI G_GGCC'C
Cuts at: 0 434 748 794 932 1365
Size: 434 314 46 138 433
Fragments arranged by size:
434 433 314 138 46
ApoI r'AATT_y
Cuts at: 0 29 1365
Size: 29 1336
AvaI C'yCGr_G
Cuts at: 0 853 1365
Size: 853 512
BamHI G'GATC_C
Cuts at: 0 538 556 1365
Size: 538 18 809
Fragments arranged by size:
809 538 18
BanI G'GyrC_C
Cuts at: 0 1117 1365
Size: 1117 248
BanII G_rGCy'C
Cuts at: 0 434 523 748 794 932 1136 1365
Size: 434 89 225 46 138 204 229
Fragments arranged by size:
434 229 225 204 138 89 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 699 1365
Size: 699 666
BglI GCCn_nnn'nGGC
Cuts at: 0 752 1365
Size: 752 613
BglII A'GATC_T
Cuts at: 0 1030 1365
Size: 1030 335
Bme1580I G_kGCm'C
Cuts at: 0 434 748 794 932 1365
Size: 434 314 46 138 433
Fragments arranged by size:
434 433 314 138 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 926 1317 1365
Size: 926 391 48
Fragments arranged by size:
926 391 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 845 1365
Size: 277 568 520
Fragments arranged by size:
568 520 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1365
Size: 1003 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 1016 1276 1301 1365
Size: 253 763 260 25 64
Fragments arranged by size:
763 260 253 64 25
BsaHI Gr'CG_yC
Cuts at: 0 163 1365
Size: 163 1202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 153 183 1324 1354 1365
Size: 153 30 1141 30 11
Fragments arranged by size:
1141 153 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 158 991 1165 1365
Size: 158 833 174 200
Fragments arranged by size:
833 200 174 158
BseYI C'CCAG_C
Cuts at: 0 112 1365
Size: 112 1253
Bsp1286I G_dGCh'C
Cuts at: 0 434 523 748 794 932 1136 1365
Size: 434 89 225 46 138 204 229
Fragments arranged by size:
434 229 225 204 138 89 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 816 1319 1365
Size: 816 503 46
Fragments arranged by size:
816 503 46
BsrDI GCAATG_nn'
Cuts at: 0 1007 1321 1365
Size: 1007 314 44
Fragments arranged by size:
1007 314 44
BsrFI r'CCGG_y
Cuts at: 0 1226 1365
Size: 1226 139
BstEII G'GTnAC_C
Cuts at: 0 475 574 1222 1365
Size: 475 99 648 143
Fragments arranged by size:
648 475 143 99
BstYI r'GATC_y
Cuts at: 0 538 556 1030 1365
Size: 538 18 474 335
Fragments arranged by size:
538 474 335 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1187 1365
Size: 1187 178
BtsI GCAGTG_nn'
Cuts at: 0 1100 1365
Size: 1100 265
DrdI GACnn_nn'nnGTC
Cuts at: 0 267 1365
Size: 267 1098
EaeI y'GGCC_r
Cuts at: 0 138 207 375 442 780 1365
Size: 138 69 168 67 338 585
Fragments arranged by size:
585 338 168 138 69 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 183 277 473 845 1365
Size: 22 161 94 196 372 520
Fragments arranged by size:
520 372 196 161 94 22
EcoO109I rG'GnC_Cy
Cuts at: 0 448 457 583 682 790 791 799 829
Size: 448 9 126 99 108 1 8 30
Cuts at: 829 1365
Size: 536
Fragments arranged by size:
536 448 126 108 99 30 9 8
1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 1015 1047 1365
Size: 153 32 830 32 318
Fragments arranged by size:
830 318 153 32 32
HincII GTy'rAC
Cuts at: 0 775 966 1365
Size: 775 191 399
Fragments arranged by size:
775 399 191
MscI TGG'CCA
Cuts at: 0 140 444 1365
Size: 140 304 921
Fragments arranged by size:
921 304 140
MslI CAynn'nnrTG
Cuts at: 0 1229 1235 1365
Size: 1229 6 130
Fragments arranged by size:
1229 130 6
MspA1I CmG'CkG
Cuts at: 0 1074 1196 1365
Size: 1074 122 169
Fragments arranged by size:
1074 169 122
NdeI CA'TA_TG
Cuts at: 0 1302 1365
Size: 1302 63
NspI r_CATG'y
Cuts at: 0 152 282 426 1280 1365
Size: 152 130 144 854 85
Fragments arranged by size:
854 152 144 130 85
PasI CC'CwG_GG
Cuts at: 0 301 318 461 1294 1365
Size: 301 17 143 833 71
Fragments arranged by size:
833 301 143 71 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 440 1365
Size: 440 925
PfoI T'CCnGG_A
Cuts at: 0 615 861 1365
Size: 615 246 504
Fragments arranged by size:
615 504 246
PpuMI rG'GwC_Cy
Cuts at: 0 457 583 682 829 1365
Size: 457 126 99 147 536
Fragments arranged by size:
536 457 147 126 99
PshAI GACnn'nnGTC
Cuts at: 0 581 1365
Size: 581 784
PsiI TTA'TAA
Cuts at: 0 823 1365
Size: 823 542
PspOMI G'GGCC_C
Cuts at: 0 430 744 790 928 1365
Size: 430 314 46 138 437
Fragments arranged by size:
437 430 314 138 46
PstI C_TGCA'G
Cuts at: 0 813 1107 1142 1365
Size: 813 294 35 223
Fragments arranged by size:
813 294 223 35
PvuII CAG'CTG
Cuts at: 0 1074 1365
Size: 1074 291
SanDI GG'GwC_CC
Cuts at: 0 583 829 1365
Size: 583 246 536
Fragments arranged by size:
583 536 246
SbfI CC_TGCA'GG
Cuts at: 0 813 1365
Size: 813 552
ScaI AGT'ACT
Cuts at: 0 1327 1365
Size: 1327 38
SfcI C'TryA_G
Cuts at: 0 809 1103 1138 1365
Size: 809 294 35 227
Fragments arranged by size:
809 294 227 35
SmaI CCC'GGG
Cuts at: 0 855 1365
Size: 855 510
SmlI C'TyrA_G
Cuts at: 0 982 1365
Size: 982 383
SphI G_CATG'C
Cuts at: 0 152 282 426 1365
Size: 152 130 144 939
Fragments arranged by size:
939 152 144 130
StyI C'CwwG_G
Cuts at: 0 524 1053 1365
Size: 524 529 312
Fragments arranged by size:
529 524 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1168 1336 1365
Size: 1012 156 168 29
Fragments arranged by size:
1012 168 156 29
TatI w'GTAC_w
Cuts at: 0 1037 1325 1365
Size: 1037 288 40
Fragments arranged by size:
1037 288 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 167 199 1365
Size: 167 32 1166
Fragments arranged by size:
1166 167 32
XmaI C'CCGG_G
Cuts at: 0 853 1365
Size: 853 512
Enzymes that do cut:
AarI AccI AcuI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BsaXI BseRI BseYI Bsp1286I BspMI
BsrDI BsrFI BstEII BstYI BtgZI BtsI DrdI EaeI
Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I NdeI
NspI PasI PflMI PfoI PpuMI PshAI PsiI PspOMI
PstI PvuII SanDI SbfI ScaI SfcI SmaI SmlI
SphI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI