Restriction map for
pFN21AE2433
(Linear) (Six-base) MAPSORT of: KIEE2433.seq Check: 4346 from: 1 to: 3912
KIEE2433.orf
With 182 enzymes: SgfI *
February 22, 2013 15:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1364 2159 2386 2981 3010 3912
Size: 1132 232 795 227 595 29 902
Fragments arranged by size:
1132 902 795 595 232 227 29
AflII C'TTAA_G
Cuts at: 0 687 3912
Size: 687 3225
AflIII A'CryG_T
Cuts at: 0 3364 3581 3912
Size: 3364 217 331
Fragments arranged by size:
3364 331 217
AhdI GACnn_n'nnGTC
Cuts at: 0 707 3912
Size: 707 3205
AleI CACnn'nnGTG
Cuts at: 0 2921 3912
Size: 2921 991
AlwNI CAG_nnn'CTG
Cuts at: 0 3759 3912
Size: 3759 153
ApoI r'AATT_y
Cuts at: 0 50 125 1573 2326 3800 3912
Size: 50 75 1448 753 1474 112
Fragments arranged by size:
1474 1448 753 112 75 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2495 2528 3912
Size: 2495 33 1384
Fragments arranged by size:
2495 1384 33
BamHI G'GATC_C
Cuts at: 0 762 3912
Size: 762 3150
BanI G'GyrC_C
Cuts at: 0 625 3912
Size: 625 3287
BanII G_rGCy'C
Cuts at: 0 3194 3699 3912
Size: 3194 505 213
Fragments arranged by size:
3194 505 213
BbsI GAAGACnn'nnnn_
Cuts at: 0 427 2893 3912
Size: 427 2466 1019
Fragments arranged by size:
2466 1019 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2083 2117 3912
Size: 2083 34 1795
Fragments arranged by size:
2083 1795 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 151 1667 2212 3912
Size: 151 1516 545 1700
Fragments arranged by size:
1700 1516 545 151
BclI T'GATC_A
Cuts at: 0 612 2652 3912
Size: 612 2040 1260
Fragments arranged by size:
2040 1260 612
BfrBI ATG'CAT
Cuts at: 0 3018 3912
Size: 3018 894
BglI GCCn_nnn'nGGC
Cuts at: 0 262 845 3912
Size: 262 583 3067
Fragments arranged by size:
3067 583 262
BglII A'GATC_T
Cuts at: 0 2792 3042 3912
Size: 2792 250 870
Fragments arranged by size:
2792 870 250
BlpI GC'TnA_GC
Cuts at: 0 314 3912
Size: 314 3598
BmtI G_CTAG'C
Cuts at: 0 517 3912
Size: 517 3395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 112 923 1151 3912
Size: 112 811 228 2761
Fragments arranged by size:
2761 811 228 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 755 1007 1034 1097 1235 1262 3912
Size: 554 201 252 27 63 138 27 2650
Fragments arranged by size:
2650 554 252 201 138 63 27 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1651 3912
Size: 68 1583 2261
Fragments arranged by size:
2261 1583 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 1833 3533 3912
Size: 658 1175 1700 379
Fragments arranged by size:
1700 1175 658 379
BsaAI yAC'GTr
Cuts at: 0 162 3912
Size: 162 3750
BsaBI GATnn'nnATC
Cuts at: 0 2311 2651 3912
Size: 2311 340 1261
Fragments arranged by size:
2311 1261 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 966 996 1095 1125 1284 1314 3912
Size: 966 30 99 30 159 30 2598
Fragments arranged by size:
2598 966 159 99 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 929 1238 3659 3912
Size: 929 309 2421 253
Fragments arranged by size:
2421 929 309 253
BseYI C'CCAG_C
Cuts at: 0 569 3768 3912
Size: 569 3199 144
Fragments arranged by size:
3199 569 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2555 3587 3912
Size: 2555 1032 325
Fragments arranged by size:
2555 1032 325
BsiHKAI G_wGCw'C
Cuts at: 0 320 864 2988 3194 3912
Size: 320 544 2124 206 718
Fragments arranged by size:
2124 718 544 320 206
BsmI GAATG_Cn'
Cuts at: 0 447 1919 2975 3912
Size: 447 1472 1056 937
Fragments arranged by size:
1472 1056 937 447
Bsp1286I G_dGCh'C
Cuts at: 0 320 864 2988 3194 3699 3912
Size: 320 544 2124 206 505 213
Fragments arranged by size:
2124 544 505 320 213 206
BspHI T'CATG_A
Cuts at: 0 2882 3270 3912
Size: 2882 388 642
Fragments arranged by size:
2882 642 388
BsrDI GCAATG_nn'
Cuts at: 0 265 1543 2123 3912
Size: 265 1278 580 1789
Fragments arranged by size:
1789 1278 580 265
BsrGI T'GTAC_A
Cuts at: 0 1679 2001 3546 3912
Size: 1679 322 1545 366
Fragments arranged by size:
1679 1545 366 322
BssSI C'ACGA_G
Cuts at: 0 1568 1723 3912
Size: 1568 155 2189
Fragments arranged by size:
2189 1568 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1807 2281 3912
Size: 1807 474 1631
Fragments arranged by size:
1807 1631 474
BstBI TT'CG_AA
Cuts at: 0 1272 3912
Size: 1272 2640
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1381 2559 3912
Size: 1098 283 1178 1353
Fragments arranged by size:
1353 1178 1098 283
BstYI r'GATC_y
Cuts at: 0 762 1718 2792 3042 3912
Size: 762 956 1074 250 870
Fragments arranged by size:
1074 956 870 762 250
BtgI C'CryG_G
Cuts at: 0 840 3912
Size: 840 3072
BtsI GCAGTG_nn'
Cuts at: 0 3575 3912
Size: 3575 337
DraI TTT'AAA
Cuts at: 0 1400 1505 2445 2819 3912
Size: 1400 105 940 374 1093
Fragments arranged by size:
1400 1093 940 374 105
EaeI y'GGCC_r
Cuts at: 0 2120 3912
Size: 2120 1792
EarI CTCTTCn'nnn_
Cuts at: 0 1463 2945 3912
Size: 1463 1482 967
Fragments arranged by size:
1482 1463 967
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 384 2194 3912
Size: 384 1810 1718
Fragments arranged by size:
1810 1718 384
EcoICRI GAG'CTC
Cuts at: 0 3192 3912
Size: 3192 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 755 1007 1034 1097 1132 1235 1262
Size: 554 201 252 27 63 35 103 27
Cuts at: 1262 1364 2159 2386 2981 3010 3912
Size: 102 795 227 595 29 902
Fragments arranged by size:
902 795 595 554 252 227 201 103
102 63 35 29 27 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 806 1112 3912
Size: 806 306 2800
Fragments arranged by size:
2800 806 306
EcoO109I rG'GnC_Cy
Cuts at: 0 3705 3912
Size: 3705 207
EcoRI G'AATT_C
Cuts at: 0 2326 3912
Size: 2326 1586
EcoRV GAT'ATC
Cuts at: 0 250 3912
Size: 250 3662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 414 446 693 725 1284 1316
Size: 196 32 186 32 247 32 559 32
Cuts at: 1316 1363 1395 1593 1625 2418 2450 3215 3247
Size: 47 32 198 32 793 32 765 32
Cuts at: 3247 3654 3686 3912
Size: 407 32 226
Fragments arranged by size:
793 765 559 407 247 226 198 196
186 47 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 290 3234 3912
Size: 290 2944 678
Fragments arranged by size:
2944 678 290
MfeI C'AATT_G
Cuts at: 0 170 1076 2612 3912
Size: 170 906 1536 1300
Fragments arranged by size:
1536 1300 906 170
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 3912
Size: 1327 2585
MscI TGG'CCA
Cuts at: 0 2122 3912
Size: 2122 1790
MslI CAynn'nnrTG
Cuts at: 0 920 1096 2921 3013 3912
Size: 920 176 1825 92 899
Fragments arranged by size:
1825 920 899 176 92
MspA1I CmG'CkG
Cuts at: 0 610 787 1255 2854 3912
Size: 610 177 468 1599 1058
Fragments arranged by size:
1599 1058 610 468 177
NcoI C'CATG_G
Cuts at: 0 840 3912
Size: 840 3072
NdeI CA'TA_TG
Cuts at: 0 1707 3912
Size: 1707 2205
NheI G'CTAG_C
Cuts at: 0 513 3912
Size: 513 3399
NsiI A_TGCA'T
Cuts at: 0 3020 3912
Size: 3020 892
NspI r_CATG'y
Cuts at: 0 1338 3368 3585 3912
Size: 1338 2030 217 327
Fragments arranged by size:
2030 1338 327 217
PasI CC'CwG_GG
Cuts at: 0 670 1914 3912
Size: 670 1244 1998
Fragments arranged by size:
1998 1244 670
PciI A'CATG_T
Cuts at: 0 3364 3581 3912
Size: 3364 217 331
Fragments arranged by size:
3364 331 217
PflMI CCAn_nnn'nTGG
Cuts at: 0 841 3912
Size: 841 3071
PfoI T'CCnGG_A
Cuts at: 0 732 801 984 2551 3912
Size: 732 69 183 1567 1361
Fragments arranged by size:
1567 1361 732 183 69
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1266 1298 3912
Size: 1266 32 2614
Fragments arranged by size:
2614 1266 32
PvuII CAG'CTG
Cuts at: 0 610 1255 2854 3912
Size: 610 645 1599 1058
Fragments arranged by size:
1599 1058 645 610
SacI G_AGCT'C
Cuts at: 0 3194 3912
Size: 3194 718
SapI GCTCTTCn'nnn_
Cuts at: 0 1463 3912
Size: 1463 2449
ScaI AGT'ACT
Cuts at: 0 3383 3912
Size: 3383 529
SexAI A'CCwGG_T
Cuts at: 0 705 3912
Size: 705 3207
SfcI C'TryA_G
Cuts at: 0 104 2711 3338 3912
Size: 104 2607 627 574
Fragments arranged by size:
2607 627 574 104
SmlI C'TyrA_G
Cuts at: 0 83 687 1630 3912
Size: 83 604 943 2282
Fragments arranged by size:
2282 943 604 83
SspI AAT'ATT
Cuts at: 0 3745 3912
Size: 3745 167
StyI C'CwwG_G
Cuts at: 0 840 2132 3912
Size: 840 1292 1780
Fragments arranged by size:
1780 1292 840
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1027 1303 2911 2935 3782 3912
Size: 1027 276 1608 24 847 130
Fragments arranged by size:
1608 1027 847 276 130 24
TatI w'GTAC_w
Cuts at: 0 1679 2001 2588 3090 3381 3546 3912
Size: 1679 322 587 502 291 165 366
Fragments arranged by size:
1679 587 502 366 322 291 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2097 2129 2382 2414 3912
Size: 2097 32 253 32 1498
Fragments arranged by size:
2097 1498 253 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 127 2115 2130 3912
Size: 127 1988 15 1782
Fragments arranged by size:
1988 1782 127 15
XmnI GAAnn'nnTTC
Cuts at: 0 1145 3912
Size: 1145 2767
Enzymes that do cut:
AcuI AflII AflIII AhdI AleI AlwNI ApoI BaeI
BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtsI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PciI PflMI PfoI PpiI PvuII SacI
SapI ScaI SexAI SfcI SmlI SspI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BbeI BbvCI Bme1580I BmgBI BmrI Bpu10I BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI NaeI NarI NgoMIV NotI NruI
PacI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI