Restriction map for
pFN21AE2424
(Linear) (Six-base) MAPSORT of: KIEE2424.seq Check: 3166 from: 1 to: 2487
KIEE2424.orf
With 182 enzymes: SgfI *
November 9, 2012 16:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1786 2487
Size: 557 1229 701
Fragments arranged by size:
1229 701 557
AfeI AGC'GCT
Cuts at: 0 1014 1137 2487
Size: 1014 123 1350
Fragments arranged by size:
1350 1014 123
AgeI A'CCGG_T
Cuts at: 0 807 2487
Size: 807 1680
AleI CACnn'nnGTG
Cuts at: 0 115 2257 2487
Size: 115 2142 230
Fragments arranged by size:
2142 230 115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2077 2487
Size: 2077 410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 2487
Size: 367 32 2088
Fragments arranged by size:
2088 367 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2306 2487
Size: 2306 181
ApaI G_GGCC'C
Cuts at: 0 74 1581 2487
Size: 74 1507 906
Fragments arranged by size:
1507 906 74
AvaI C'yCGr_G
Cuts at: 0 1034 1181 1974 2487
Size: 1034 147 793 513
Fragments arranged by size:
1034 793 513 147
BamHI G'GATC_C
Cuts at: 0 1616 2487
Size: 1616 871
BanI G'GyrC_C
Cuts at: 0 231 282 821 1784 2487
Size: 231 51 539 963 703
Fragments arranged by size:
963 703 539 231 51
BanII G_rGCy'C
Cuts at: 0 74 533 775 1201 1581 2487
Size: 74 459 242 426 380 906
Fragments arranged by size:
906 459 426 380 242 74
BbeI G_GCGC'C
Cuts at: 0 235 2487
Size: 235 2252
BbsI GAAGACnn'nnnn_
Cuts at: 0 754 761 979 1356 1890 2475 2487
Size: 754 7 218 377 534 585 12
Fragments arranged by size:
754 585 534 377 218 12 7
BbvCI CC'TCA_GC
Cuts at: 0 1501 1933 2487
Size: 1501 432 554
Fragments arranged by size:
1501 554 432
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2340 2374 2487
Size: 2340 34 113
Fragments arranged by size:
2340 113 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2448 2487
Size: 2448 39
BclI T'GATC_A
Cuts at: 0 1394 2487
Size: 1394 1093
BglI GCCn_nnn'nGGC
Cuts at: 0 1828 2487
Size: 1828 659
BglII A'GATC_T
Cuts at: 0 1331 2474 2487
Size: 1331 1143 13
Fragments arranged by size:
1331 1143 13
Bme1580I G_kGCm'C
Cuts at: 0 74 287 824 1581 1605 1654 1702 2487
Size: 74 213 537 757 24 49 48 785
Fragments arranged by size:
785 757 537 213 74 49 48 24
BmgBI CAC'GTC
Cuts at: 0 866 2487
Size: 866 1621
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 507 1154 1480 2487
Size: 482 25 647 326 1007
Fragments arranged by size:
1007 647 482 326 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 978 2487
Size: 978 1509
Bpu10I CC'TnA_GC
Cuts at: 0 466 1501 1933 2487
Size: 466 1035 432 554
Fragments arranged by size:
1035 554 466 432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 2487
Size: 839 1648
BsaAI yAC'GTr
Cuts at: 0 117 393 2001 2046 2487
Size: 117 276 1608 45 441
Fragments arranged by size:
1608 441 276 117 45
BsaHI Gr'CG_yC
Cuts at: 0 95 232 2487
Size: 95 137 2255
Fragments arranged by size:
2255 137 95
BsaWI w'CCGG_w
Cuts at: 0 807 2204 2487
Size: 807 1397 283
Fragments arranged by size:
1397 807 283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 367 397 1075 1105 2380 2410 2487
Size: 367 30 678 30 1275 30 77
Fragments arranged by size:
1275 678 367 77 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 1921 2347 2388 2487
Size: 1222 699 426 41 99
Fragments arranged by size:
1222 699 426 99 41
BseYI C'CCAG_C
Cuts at: 0 715 947 1524 1854 2487
Size: 715 232 577 330 633
Fragments arranged by size:
715 633 577 330 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 2049 2482 2487
Size: 1405 644 433 5
Fragments arranged by size:
1405 644 433 5
BsiEI CG_ry'CG
Cuts at: 0 47 812 2487
Size: 47 765 1675
Fragments arranged by size:
1675 765 47
BsiHKAI G_wGCw'C
Cuts at: 0 32 467 533 1201 2006 2339 2487
Size: 32 435 66 668 805 333 148
Fragments arranged by size:
805 668 435 333 148 66 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 87 2487
Size: 87 2400
Bsp1286I G_dGCh'C
Cuts at: 0 32 74 287 467 533 775 824 1201
Size: 32 42 213 180 66 242 49 377
Cuts at: 1201 1581 1605 1654 1702 2006 2339 2487
Size: 380 24 49 48 304 333 148
Fragments arranged by size:
380 377 333 304 242 213 180 148
66 49 49 48 42 32 24
BspEI T'CCGG_A
Cuts at: 0 2204 2487
Size: 2204 283
BspHI T'CATG_A
Cuts at: 0 2195 2487
Size: 2195 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 932 2174 2487
Size: 932 1242 313
Fragments arranged by size:
1242 932 313
BsrBI CCG'CTC
Cuts at: 0 2040 2487
Size: 2040 447
BsrDI GCAATG_nn'
Cuts at: 0 1346 1355 1596 2487
Size: 1346 9 241 891
Fragments arranged by size:
1346 891 241 9
BsrFI r'CCGG_y
Cuts at: 0 807 1958 2487
Size: 807 1151 529
Fragments arranged by size:
1151 807 529
BsrGI T'GTAC_A
Cuts at: 0 1739 2487
Size: 1739 748
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2249 2487
Size: 2249 238
BstEII G'GTnAC_C
Cuts at: 0 564 1027 1162 2487
Size: 564 463 135 1325
Fragments arranged by size:
1325 564 463 135
BstYI r'GATC_y
Cuts at: 0 1111 1331 1616 1979 2474 2487
Size: 1111 220 285 363 495 13
Fragments arranged by size:
1111 495 363 285 220 13
BtgI C'CryG_G
Cuts at: 0 1646 2258 2487
Size: 1646 612 229
Fragments arranged by size:
1646 612 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1902 2388 2487
Size: 1902 486 99
Fragments arranged by size:
1902 486 99
BtsI GCAGTG_nn'
Cuts at: 0 2032 2487
Size: 2032 455
ClaI AT'CG_AT
Cuts at: 0 1238 2487
Size: 1238 1249
DrdI GACnn_nn'nnGTC
Cuts at: 0 1252 2487
Size: 1252 1235
EaeI y'GGCC_r
Cuts at: 0 686 986 1829 2147 2487
Size: 686 300 843 318 340
Fragments arranged by size:
843 686 340 318 300
EarI CTCTTCn'nnn_
Cuts at: 0 2284 2487
Size: 2284 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 241 1975 2487
Size: 241 1734 512
Fragments arranged by size:
1734 512 241
EcoICRI GAG'CTC
Cuts at: 0 531 1199 2487
Size: 531 668 1288
Fragments arranged by size:
1288 668 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 978 1786 2487
Size: 557 421 808 701
Fragments arranged by size:
808 701 557 421
EcoO109I rG'GnC_Cy
Cuts at: 0 70 1149 1578 2417 2487
Size: 70 1079 429 839 70
Fragments arranged by size:
1079 839 429 70 70
FspI TGC'GCA
Cuts at: 0 1588 2487
Size: 1588 899
HaeII r_GCGC'y
Cuts at: 0 235 1016 1139 1487 1808 2487
Size: 235 781 123 348 321 679
Fragments arranged by size:
781 679 348 321 235 123
HincII GTy'rAC
Cuts at: 0 129 144 420 1131 2487
Size: 129 15 276 711 1356
Fragments arranged by size:
1356 711 276 129 15
KasI G'GCGC_C
Cuts at: 0 231 2487
Size: 231 2256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 596 1268 2487
Size: 353 243 672 1219
Fragments arranged by size:
1219 672 353 243
MscI TGG'CCA
Cuts at: 0 988 1831 2149 2487
Size: 988 843 318 338
Fragments arranged by size:
988 843 338 318
MslI CAynn'nnrTG
Cuts at: 0 115 658 1741 2194 2257 2407 2487
Size: 115 543 1083 453 63 150 80
Fragments arranged by size:
1083 543 453 150 115 80 63
MspA1I CmG'CkG
Cuts at: 0 1389 2033 2466 2487
Size: 1389 644 433 21
Fragments arranged by size:
1389 644 433 21
NarI GG'CG_CC
Cuts at: 0 232 2487
Size: 232 2255
NcoI C'CATG_G
Cuts at: 0 1646 2487
Size: 1646 841
NspI r_CATG'y
Cuts at: 0 663 1764 2487
Size: 663 1101 723
Fragments arranged by size:
1101 723 663
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 1410 1470 2487
Size: 333 1077 60 1017
Fragments arranged by size:
1077 1017 333 60
PmlI CAC'GTG
Cuts at: 0 2001 2487
Size: 2001 486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 2487
Size: 367 32 2088
Fragments arranged by size:
2088 367 32
PpuMI rG'GwC_Cy
Cuts at: 0 1149 2417 2487
Size: 1149 1268 70
Fragments arranged by size:
1268 1149 70
PspOMI G'GGCC_C
Cuts at: 0 70 1577 2487
Size: 70 1507 910
Fragments arranged by size:
1507 910 70
PspXI vC'TCGA_Gb
Cuts at: 0 1034 2487
Size: 1034 1453
PstI C_TGCA'G
Cuts at: 0 2325 2487
Size: 2325 162
PvuII CAG'CTG
Cuts at: 0 1389 2487
Size: 1389 1098
SacI G_AGCT'C
Cuts at: 0 533 1201 2487
Size: 533 668 1286
Fragments arranged by size:
1286 668 533
SapI GCTCTTCn'nnn_
Cuts at: 0 2284 2487
Size: 2284 203
ScaI AGT'ACT
Cuts at: 0 517 2487
Size: 517 1970
SfcI C'TryA_G
Cuts at: 0 1809 2321 2487
Size: 1809 512 166
Fragments arranged by size:
1809 512 166
SfoI GGC'GCC
Cuts at: 0 233 2487
Size: 233 2254
SmlI C'TyrA_G
Cuts at: 0 854 1034 2487
Size: 854 180 1453
Fragments arranged by size:
1453 854 180
SphI G_CATG'C
Cuts at: 0 1764 2487
Size: 1764 723
StuI AGG'CCT
Cuts at: 0 580 2487
Size: 580 1907
StyI C'CwwG_G
Cuts at: 0 1023 1646 2487
Size: 1023 623 841
Fragments arranged by size:
1023 841 623
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 766 1010 1246 1481 2487
Size: 33 733 244 236 235 1006
Fragments arranged by size:
1006 733 244 236 235 33
TatI w'GTAC_w
Cuts at: 0 515 1739 2487
Size: 515 1224 748
Fragments arranged by size:
1224 748 515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 2380 2412 2487
Size: 601 32 1747 32 75
Fragments arranged by size:
1747 601 75 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 124 2487
Size: 124 2363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1200 2487
Size: 1200 1287
XhoI C'TCGA_G
Cuts at: 0 1034 2487
Size: 1034 1453
Enzymes that do cut:
AcuI AfeI AgeI AleI AlfI AloI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgI
BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FspI HaeII HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PflMI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuII SacI SapI ScaI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BfrBI
BlpI BmtI BplI BsaI BsaBI BsiWI BsmI BssHII
BssSI BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII
EagI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI XbaI XmaI XmnI ZraI