(Linear) (Six-base) MAPSORT of: KIEE2423.seq  Check: 2332  from: 1  to: 834



KIEE2423.orf

 With 182 enzymes: SgfI * 

                        October 29, 2012 16:51  ..

AccI GT'mk_AC

Cuts at:      0     740     834 
   Size:        740      94
 
Acc65I G'GTAC_C

Cuts at:      0     293     834 
   Size:        293     541
 
AclI AA'CG_TT

Cuts at:      0     451     834 
   Size:        451     383
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     785     834 
   Size:        520     265      49

  Fragments arranged by size: 

                 520     265      49
 
AleI CACnn'nnGTG

Cuts at:      0     182     834 
   Size:        182     652
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     482     834 
   Size:        482     352
 
ApoI r'AATT_y

Cuts at:      0     151     443     589     718     834 
   Size:        151     292     146     129     116

  Fragments arranged by size: 

                 292     151     146     129     116
 
BamHI G'GATC_C

Cuts at:      0     607     834 
   Size:        607     227
 
BanI G'GyrC_C

Cuts at:      0     293     834 
   Size:        293     541
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     315     327     834 
   Size:        315      12     507

  Fragments arranged by size: 

                 507     315      12
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     118     834 
   Size:        118     716
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     303     640     834 
   Size:        303     337     194

  Fragments arranged by size: 

                 337     303     194
 
BstBI TT'CG_AA

Cuts at:      0     147     834 
   Size:        147     687
 
BstYI r'GATC_y

Cuts at:      0     388     607     834 
   Size:        388     219     227

  Fragments arranged by size: 

                 388     227     219
 
BstZ17I GTA'TAC

Cuts at:      0     741     834 
   Size:        741      93
 
BtgI C'CryG_G

Cuts at:      0     313     834 
   Size:        313     521
 
DraI TTT'AAA

Cuts at:      0      96     834 
   Size:         96     738
 
EarI CTCTTCn'nnn_

Cuts at:      0      81     746     834 
   Size:         81     665      88

  Fragments arranged by size: 

                 665      88      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     785     834 
   Size:        520     265      49

  Fragments arranged by size: 

                 520     265      49
 
EcoRI G'AATT_C

Cuts at:      0     718     834 
   Size:        718     116
 
EcoRV GAT'ATC

Cuts at:      0     681     834 
   Size:        681     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624     834 
   Size:        592      32     210

  Fragments arranged by size: 

                 592     210      32
 
HincII GTy'rAC

Cuts at:      0     632     834 
   Size:        632     202
 
KpnI G_GTAC'C

Cuts at:      0     297     834 
   Size:        297     537
 
MslI CAynn'nnrTG

Cuts at:      0     182     834 
   Size:        182     652
 
StuI AGG'CCT

Cuts at:      0     824     834 
   Size:        824      10
 
StyI C'CwwG_G

Cuts at:      0     433     834 
   Size:        433     401
 
XmnI GAAnn'nnTTC

Cuts at:      0      18     173     834 
   Size:         18     155     661

  Fragments arranged by size: 

                 661     155      18
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AleI     AlfI     ApoI    BamHI
     BanI     BbsI    BciVI    BseRI    BstBI    BstYI  BstZ17I     BtgI
     DraI     EarI  Eco57MI    EcoRI    EcoRV    Hin4I   HincII     KpnI
     MslI     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI