(Linear) (Six-base) MAPSORT of: KIEE2414.seq  Check: 1134  from: 1  to: 453



KIEE2414.orf

 With 182 enzymes: SgfI * 

                        June 21, 2012 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     453 
   Size:        234     219
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363     453 
   Size:        363      90
 
ApaI G_GGCC'C

Cuts at:      0      57     453 
   Size:         57     396
 
BanI G'GyrC_C

Cuts at:      0     111     453 
   Size:        111     342
 
BanII G_rGCy'C

Cuts at:      0      57     208     453 
   Size:         57     151     245

  Fragments arranged by size: 

                 245     151      57
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43      52     175     279     453 
   Size:         43       9     123     104     174

  Fragments arranged by size: 

                 174     123     104      43       9
 
BlpI GC'TnA_GC

Cuts at:      0      95     269     453 
   Size:         95     174     184

  Fragments arranged by size: 

                 184     174      95
 
Bme1580I G_kGCm'C

Cuts at:      0      57     221     375     453 
   Size:         57     164     154      78

  Fragments arranged by size: 

                 164     154      78      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     251     453 
   Size:        251     202
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     453 
   Size:        385      68
 
BseYI C'CCAG_C

Cuts at:      0     314     453 
   Size:        314     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     453 
   Size:         56     397
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     208     221     375     453 
   Size:         57     151      13     154      78

  Fragments arranged by size: 

                 154     151      78      57      13
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     184     453 
   Size:        184     269
 
Bsu36I CC'TnA_GG

Cuts at:      0     305     453 
   Size:        305     148
 
BtsI GCAGTG_nn'

Cuts at:      0     182     453 
   Size:        182     271
 
EaeI y'GGCC_r

Cuts at:      0      44     453 
   Size:         44     409
 
EarI CTCTTCn'nnn_

Cuts at:      0     138     453 
   Size:        138     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     234     385     453 
   Size:        234     151      68

  Fragments arranged by size: 

                 234     151      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     453 
   Size:         54     399
 
MspA1I CmG'CkG

Cuts at:      0     109     190     453 
   Size:        109      81     263

  Fragments arranged by size: 

                 263     109      81
 
PspOMI G'GGCC_C

Cuts at:      0      53     453 
   Size:         53     400
 
PstI C_TGCA'G

Cuts at:      0     126     189     453 
   Size:        126      63     264

  Fragments arranged by size: 

                 264     126      63
 
PvuII CAG'CTG

Cuts at:      0     190     453 
   Size:        190     263
 
SapI GCTCTTCn'nnn_

Cuts at:      0     138     453 
   Size:        138     315
 
SfcI C'TryA_G

Cuts at:      0     122     185     453 
   Size:        122      63     268

  Fragments arranged by size: 

                 268     122      63
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      43      52     453 
   Size:         43       9     401

  Fragments arranged by size: 

                 401      43       9
 
StuI AGG'CCT

Cuts at:      0     282     453 
   Size:        282     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      90     453 
   Size:         90     363
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     BanI    BanII     BglI     BlpI Bme1580I
     BmrI     BpmI    BseYI     BsgI Bsp1286I   BstAPI   Bsu36I     BtsI
     EaeI     EarI  Eco57MI EcoO109I   MspA1I   PspOMI     PstI    PvuII
     SapI     SfcI     SfiI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI