(Linear) (Six-base) MAPSORT of: KIEE2412.seq  Check: 3049  from: 1  to: 528



KIEE2412.orf

 With 182 enzymes: SgfI * 

                        May 24, 2012 13:15  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     118     150     528 
   Size:        118      32     378

  Fragments arranged by size: 

                 378     118      32
 
AvaI C'yCGr_G

Cuts at:      0      62     105     528 
   Size:         62      43     423

  Fragments arranged by size: 

                 423      62      43
 
BanI G'GyrC_C

Cuts at:      0     109     528 
   Size:        109     419
 
BbeI G_GCGC'C

Cuts at:      0     113     528 
   Size:        113     415
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324     528 
   Size:        324     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     528 
   Size:        145     383
 
BsaHI Gr'CG_yC

Cuts at:      0     110     528 
   Size:        110     418
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       5      35     118     148     528 
   Size:          5      30      83      30     380

  Fragments arranged by size: 

                 380      83      30      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     229     528 
   Size:        156      73     299

  Fragments arranged by size: 

                 299     156      73
 
BsiEI CG_ry'CG

Cuts at:      0       8     526     528 
   Size:          8     518       2

  Fragments arranged by size: 

                 518       8       2
 
BsrDI GCAATG_nn'

Cuts at:      0     387     528 
   Size:        387     141
 
BsrFI r'CCGG_y

Cuts at:      0       7      79     528 
   Size:          7      72     449

  Fragments arranged by size: 

                 449      72       7
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     384     528 
   Size:        384     144
 
BstEII G'GTnAC_C

Cuts at:      0      66     528 
   Size:         66     462
 
EaeI y'GGCC_r

Cuts at:      0       5     528 
   Size:          5     523
 
EagI C'GGCC_G

Cuts at:      0       5     528 
   Size:          5     523
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     528 
   Size:         26     502
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     528 
   Size:        145     383
 
HaeII r_GCGC'y

Cuts at:      0     113     528 
   Size:        113     415
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37     528 
   Size:          5      32     491

  Fragments arranged by size: 

                 491      32       5
 
KasI G'GCGC_C

Cuts at:      0     109     528 
   Size:        109     419
 
MslI CAynn'nnrTG

Cuts at:      0     208     528 
   Size:        208     320
 
NaeI GCC'GGC

Cuts at:      0       9      81     528 
   Size:          9      72     447

  Fragments arranged by size: 

                 447      72       9
 
NarI GG'CG_CC

Cuts at:      0     110     528 
   Size:        110     418
 
NgoMIV G'CCGG_C

Cuts at:      0       7      79     528 
   Size:          7      72     449

  Fragments arranged by size: 

                 449      72       7
 
NspI r_CATG'y

Cuts at:      0     255     528 
   Size:        255     273
 
PfoI T'CCnGG_A

Cuts at:      0     299     528 
   Size:        299     229
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     528 
   Size:        118      32     378

  Fragments arranged by size: 

                 378     118      32
 
SfoI GGC'GCC

Cuts at:      0     111     528 
   Size:        111     417
 
SmaI CCC'GGG

Cuts at:      0      64     528 
   Size:         64     464
 
XmaI C'CCGG_G

Cuts at:      0      62     528 
   Size:         62     466
 

 Enzymes that do cut:

     AloI     AvaI     BanI     BbeI     BbsI     BpmI    BsaHI    BsaXI
    BseRI    BsiEI    BsrDI    BsrFI   BstAPI   BstEII     EaeI     EagI
     EciI  Eco57MI    HaeII    Hin4I     KasI     MslI     NaeI     NarI
   NgoMIV     NspI     PfoI     PpiI     SfoI     SmaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI