(Linear) (Six-base) MAPSORT of: KIEE2403.seq  Check: 3795  from: 1  to: 636



KIEE2403.orf

 With 182 enzymes: SgfI * 

                        February 28, 2012 13:56  ..

AccI GT'mk_AC

Cuts at:      0      33     320     636 
   Size:         33     287     316

  Fragments arranged by size: 

                 316     287      33
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     636 
   Size:        188     448
 
ApaLI G'TGCA_C

Cuts at:      0     189     636 
   Size:        189     447
 
BamHI G'GATC_C

Cuts at:      0      63     636 
   Size:         63     573
 
BclI T'GATC_A

Cuts at:      0     144     636 
   Size:        144     492
 
BglI GCCn_nnn'nGGC

Cuts at:      0      75     636 
   Size:         75     561
 
BglII A'GATC_T

Cuts at:      0     609     636 
   Size:        609      27
 
Bme1580I G_kGCm'C

Cuts at:      0     193     636 
   Size:        193     443
 
BmgBI CAC'GTC

Cuts at:      0     150     636 
   Size:        150     486
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     171     636 
   Size:        171     465
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     327     427     636 
   Size:        314      13     100     209

  Fragments arranged by size: 

                 314     209     100      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     636 
   Size:         36     600
 
BsaAI yAC'GTr

Cuts at:      0     498     636 
   Size:        498     138
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     221     251     636 
   Size:        221      30     385

  Fragments arranged by size: 

                 385     221      30
 
BseYI C'CCAG_C

Cuts at:      0     201     636 
   Size:        201     435
 
BsiHKAI G_wGCw'C

Cuts at:      0     193     636 
   Size:        193     443
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     636 
   Size:        193     443
 
BspHI T'CATG_A

Cuts at:      0     132     636 
   Size:        132     504
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     381     636 
   Size:        381     255
 
BsrDI GCAATG_nn'

Cuts at:      0     614     636 
   Size:        614      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     211     636 
   Size:        211     425
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      94     292     636 
   Size:         94     198     344

  Fragments arranged by size: 

                 344     198      94
 
BstYI r'GATC_y

Cuts at:      0      63     609     636 
   Size:         63     546      27

  Fragments arranged by size: 

                 546      63      27
 
BtsI GCAGTG_nn'

Cuts at:      0     553     636 
   Size:        553      83
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      10     636 
   Size:         10     626
 
EarI CTCTTCn'nnn_

Cuts at:      0     446     586     636 
   Size:        446     140      50

  Fragments arranged by size: 

                 446     140      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      56     636 
   Size:         56     580
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     314     327     427     636 
   Size:        188     126      13     100     209

  Fragments arranged by size: 

                 209     188     126     100      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     636 
   Size:        305     331
 
MspA1I CmG'CkG

Cuts at:      0     292     636 
   Size:        292     344
 
PstI C_TGCA'G

Cuts at:      0     563     636 
   Size:        563      73
 
PvuII CAG'CTG

Cuts at:      0     292     636 
   Size:        292     344
 
ScaI AGT'ACT

Cuts at:      0     112     636 
   Size:        112     524
 
SfcI C'TryA_G

Cuts at:      0     559     636 
   Size:        559      77
 
SmlI C'TyrA_G

Cuts at:      0      15     636 
   Size:         15     621
 
StuI AGG'CCT

Cuts at:      0     517     636 
   Size:        517     119
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     636 
   Size:        190     446
 
TatI w'GTAC_w

Cuts at:      0     110     636 
   Size:        110     526
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     636 
   Size:        221      32     383

  Fragments arranged by size: 

                 383     221      32
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI    BamHI     BclI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI    BpuEI    BsaAI    BsaXI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrDI   BstAPI    BstXI    BstYI     BtsI
     DrdI     EarI     EciI  Eco57MI     MmeI   MspA1I     PstI    PvuII
     ScaI     SfcI     SmlI     StuI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BlpI     BmtI     BplI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI