Restriction map for
pFN21AE2402
(Linear) (Six-base) MAPSORT of: KIEE2402.seq Check: 4668 from: 1 to: 2082
KIEE2402.orf
With 182 enzymes: SgfI *
February 28, 2012 13:56 ..
AccI GT'mk_AC
Cuts at: 0 1959 2082
Size: 1959 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 733 2082
Size: 397 336 1349
Fragments arranged by size:
1349 397 336
AleI CACnn'nnGTG
Cuts at: 0 256 2082
Size: 256 1826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 248 2082
Size: 248 1834
AlwNI CAG_nnn'CTG
Cuts at: 0 948 1536 2082
Size: 948 588 546
Fragments arranged by size:
948 588 546
ApaI G_GGCC'C
Cuts at: 0 38 194 592 608 632 883 1139 1162
Size: 38 156 398 16 24 251 256 23
Cuts at: 1162 2033 2082
Size: 871 49
Fragments arranged by size:
871 398 256 251 156 49 38 24
23 16
ApaLI G'TGCA_C
Cuts at: 0 228 1888 2082
Size: 228 1660 194
Fragments arranged by size:
1660 228 194
ApoI r'AATT_y
Cuts at: 0 316 2082
Size: 316 1766
AvaI C'yCGr_G
Cuts at: 0 193 765 835 1070 1274 1306 2082
Size: 193 572 70 235 204 32 776
Fragments arranged by size:
776 572 235 204 193 70 32
BamHI G'GATC_C
Cuts at: 0 1255 2082
Size: 1255 827
BanI G'GyrC_C
Cuts at: 0 517 707 888 957 2082
Size: 517 190 181 69 1125
Fragments arranged by size:
1125 517 190 181 69
BanII G_rGCy'C
Cuts at: 0 38 194 592 608 632 830 883 1139
Size: 38 156 398 16 24 198 53 256
Cuts at: 1139 1162 1232 1582 2033 2082
Size: 23 70 350 451 49
Fragments arranged by size:
451 398 350 256 198 156 70 53
49 38 24 23 16
BbeI G_GCGC'C
Cuts at: 0 521 2082
Size: 521 1561
BbsI GAAGACnn'nnnn_
Cuts at: 0 1287 2082
Size: 1287 795
BbvCI CC'TCA_GC
Cuts at: 0 392 1468 2082
Size: 392 1076 614
Fragments arranged by size:
1076 614 392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 95 128 129 162 339 373 1990 2024
Size: 95 33 1 33 177 34 1617 34
Cuts at: 2024 2082
Size: 58
Fragments arranged by size:
1617 177 95 58 34 34 33 33
1
BglI GCCn_nnn'nGGC
Cuts at: 0 603 2017 2082
Size: 603 1414 65
Fragments arranged by size:
1414 603 65
BlpI GC'TnA_GC
Cuts at: 0 1581 1795 2082
Size: 1581 214 287
Fragments arranged by size:
1581 287 214
Bme1580I G_kGCm'C
Cuts at: 0 38 194 232 436 592 608 632 883
Size: 38 156 38 204 156 16 24 251
Cuts at: 883 1015 1139 1162 1461 1892 2013 2033 2082
Size: 132 124 23 299 431 121 20 49
Fragments arranged by size:
431 299 251 204 156 156 132 124
121 49 38 38 24 23 20 16
BmgBI CAC'GTC
Cuts at: 0 621 1827 1865 2082
Size: 621 1206 38 217
Fragments arranged by size:
1206 621 217 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 852 1832 2082
Size: 852 980 250
Fragments arranged by size:
980 852 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 2082
Size: 1210 872
Bpu10I CC'TnA_GC
Cuts at: 0 392 1226 1396 1468 1810 2082
Size: 392 834 170 72 342 272
Fragments arranged by size:
834 392 342 272 170 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2056 2082
Size: 2056 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 1873 2082
Size: 1873 209
BsaAI yAC'GTr
Cuts at: 0 1200 2082
Size: 1200 882
BsaHI Gr'CG_yC
Cuts at: 0 52 102 518 731 2082
Size: 52 50 416 213 1351
Fragments arranged by size:
1351 416 213 52 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 982 1006 1012 1963 1993 2082
Size: 976 6 24 6 951 30 89
Fragments arranged by size:
976 951 89 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 565 730 960 969 1840 2071 2082
Size: 526 39 165 230 9 871 231 11
Fragments arranged by size:
871 526 231 230 165 39 11 9
BseYI C'CCAG_C
Cuts at: 0 280 425 882 1161 1643 1790 2082
Size: 280 145 457 279 482 147 292
Fragments arranged by size:
482 457 292 280 279 147 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 2082
Size: 426 1656
BsiHKAI G_wGCw'C
Cuts at: 0 232 245 1544 1892 2082
Size: 232 13 1299 348 190
Fragments arranged by size:
1299 348 232 190 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 722 1774 2082
Size: 722 1052 308
Fragments arranged by size:
1052 722 308
Bsp1286I G_dGCh'C
Cuts at: 0 38 194 232 245 436 592 608 632
Size: 38 156 38 13 191 156 16 24
Cuts at: 632 830 883 1015 1139 1162 1232 1461 1544
Size: 198 53 132 124 23 70 229 83
Cuts at: 1544 1582 1892 2013 2033 2082
Size: 38 310 121 20 49
Fragments arranged by size:
310 229 198 191 156 156 132 124
121 83 70 53 49 38 38 38
24 23 20 16 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1729 1982 2082
Size: 1729 253 100
Fragments arranged by size:
1729 253 100
BsrBI CCG'CTC
Cuts at: 0 533 1194 2082
Size: 533 661 888
Fragments arranged by size:
888 661 533
BsrFI r'CCGG_y
Cuts at: 0 1400 2082
Size: 1400 682
BsrGI T'GTAC_A
Cuts at: 0 340 2082
Size: 340 1742
BssHII G'CGCG_C
Cuts at: 0 94 2082
Size: 94 1988
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1203 2082
Size: 1203 879
BstYI r'GATC_y
Cuts at: 0 413 1255 2082
Size: 413 842 827
Fragments arranged by size:
842 827 413
Bsu36I CC'TnA_GG
Cuts at: 0 2054 2082
Size: 2054 28
BtgI C'CryG_G
Cuts at: 0 1098 1312 1701 2082
Size: 1098 214 389 381
Fragments arranged by size:
1098 389 381 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1001 2082
Size: 1001 1081
BtsI GCAGTG_nn'
Cuts at: 0 230 232 1134 1588 1815 1926 2082
Size: 230 2 902 454 227 111 156
Fragments arranged by size:
902 454 230 227 156 111 2
DraIII CAC_nnn'GTG
Cuts at: 0 1203 2082
Size: 1203 879
EaeI y'GGCC_r
Cuts at: 0 486 1063 2082
Size: 486 577 1019
Fragments arranged by size:
1019 577 486
EarI CTCTTCn'nnn_
Cuts at: 0 305 978 1011 2082
Size: 305 673 33 1071
Fragments arranged by size:
1071 673 305 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1419 1933 2082
Size: 1419 514 149
Fragments arranged by size:
1419 514 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 733 1210 2082
Size: 397 336 477 872
Fragments arranged by size:
872 477 397 336
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 2082
Size: 715 1367
EcoO109I rG'GnC_Cy
Cuts at: 0 35 190 481 588 589 605 629 879
Size: 35 155 291 107 1 16 24 250
Cuts at: 879 1135 1136 1833 2030 2082
Size: 256 1 697 197 52
Fragments arranged by size:
697 291 256 250 197 155 107 52
35 24 16 1 1
HaeII r_GCGC'y
Cuts at: 0 521 1802 2082
Size: 521 1281 280
Fragments arranged by size:
1281 521 280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 955 976 982 987 988 1008 1014 1020
Size: 955 21 6 5 1 20 6 6
Cuts at: 1020 1689 1721 1963 1995 2082
Size: 669 32 242 32 87
Fragments arranged by size:
955 669 242 87 32 32 21 20
6 6 6 5 1
HindIII A'AGCT_T
Cuts at: 0 2003 2082
Size: 2003 79
KasI G'GCGC_C
Cuts at: 0 517 2082
Size: 517 1565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1025 1283 2082
Size: 1019 6 258 799
Fragments arranged by size:
1019 799 258 6
MslI CAynn'nnrTG
Cuts at: 0 256 2082
Size: 256 1826
MspA1I CmG'CkG
Cuts at: 0 113 122 256 886 945 1148 1188 1314
Size: 113 9 134 630 59 203 40 126
Cuts at: 1314 1358 1472 1619 1794 1938 1949 2023 2048
Size: 44 114 147 175 144 11 74 25
Cuts at: 2048 2082
Size: 34
Fragments arranged by size:
630 203 175 147 144 134 126 114
113 74 59 44 40 34 25 11
9
NaeI GCC'GGC
Cuts at: 0 1402 2082
Size: 1402 680
NarI GG'CG_CC
Cuts at: 0 518 2082
Size: 518 1564
NgoMIV G'CCGG_C
Cuts at: 0 1400 2082
Size: 1400 682
NspI r_CATG'y
Cuts at: 0 1335 2082
Size: 1335 747
PasI CC'CwG_GG
Cuts at: 0 782 823 1155 1575 2082
Size: 782 41 332 420 507
Fragments arranged by size:
782 507 420 332 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 425 948 2082
Size: 425 523 1134
Fragments arranged by size:
1134 523 425
PfoI T'CCnGG_A
Cuts at: 0 1607 1649 2082
Size: 1607 42 433
Fragments arranged by size:
1607 433 42
PmlI CAC'GTG
Cuts at: 0 1200 2082
Size: 1200 882
PpuMI rG'GwC_Cy
Cuts at: 0 1833 2082
Size: 1833 249
PshAI GACnn'nnGTC
Cuts at: 0 1373 1480 2082
Size: 1373 107 602
Fragments arranged by size:
1373 602 107
PspOMI G'GGCC_C
Cuts at: 0 34 190 588 604 628 879 1135 1158
Size: 34 156 398 16 24 251 256 23
Cuts at: 1158 2029 2082
Size: 871 53
Fragments arranged by size:
871 398 256 251 156 53 34 24
23 16
PstI C_TGCA'G
Cuts at: 0 876 1532 1715 2082
Size: 876 656 183 367
Fragments arranged by size:
876 656 367 183
PvuII CAG'CTG
Cuts at: 0 256 945 1148 1188 1472 1794 2023 2082
Size: 256 689 203 40 284 322 229 59
Fragments arranged by size:
689 322 284 256 229 203 59 40
SacII CC_GC'GG
Cuts at: 0 1315 2082
Size: 1315 767
SanDI GG'GwC_CC
Cuts at: 0 1833 2082
Size: 1833 249
SbfI CC_TGCA'GG
Cuts at: 0 1532 2082
Size: 1532 550
SfcI C'TryA_G
Cuts at: 0 872 1528 1711 2082
Size: 872 656 183 371
Fragments arranged by size:
872 656 371 183
SfoI GGC'GCC
Cuts at: 0 519 2082
Size: 519 1563
SmaI CCC'GGG
Cuts at: 0 195 767 1072 1276 2082
Size: 195 572 305 204 806
Fragments arranged by size:
806 572 305 204 195
SmlI C'TyrA_G
Cuts at: 0 2071 2082
Size: 2071 11
SphI G_CATG'C
Cuts at: 0 1335 2082
Size: 1335 747
SrfI GCCC'GGGC
Cuts at: 0 195 767 2082
Size: 195 572 1315
Fragments arranged by size:
1315 572 195
StuI AGG'CCT
Cuts at: 0 1526 2082
Size: 1526 556
StyI C'CwwG_G
Cuts at: 0 676 1244 2082
Size: 676 568 838
Fragments arranged by size:
838 676 568
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 712 2082
Size: 460 252 1370
Fragments arranged by size:
1370 460 252
TatI w'GTAC_w
Cuts at: 0 340 2082
Size: 340 1742
XmaI C'CCGG_G
Cuts at: 0 193 765 1070 1274 2082
Size: 193 572 305 204 808
Fragments arranged by size:
808 572 305 204 193
XmnI GAAnn'nnTTC
Cuts at: 0 181 2082
Size: 181 1901
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
SacII SanDI SbfI SfcI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BmtI BplI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BssSI BstBI BstEII
BstXI BstZ17I ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TstI
Tth111I XbaI XcmI XhoI ZraI