Restriction map for
pFN21AE2401
(Linear) (Six-base) MAPSORT of: KIEE2401.seq Check: 9739 from: 1 to: 1101
KIEE2401.orf
With 182 enzymes: SgfI *
March 8, 2012 13:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1100 1101
Size: 686 414 1
Fragments arranged by size:
686 414 1
AfeI AGC'GCT
Cuts at: 0 121 1077 1101
Size: 121 956 24
Fragments arranged by size:
956 121 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1101
Size: 1053 32 16
Fragments arranged by size:
1053 32 16
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1101
Size: 534 567
ApoI r'AATT_y
Cuts at: 0 1009 1101
Size: 1009 92
AvaI C'yCGr_G
Cuts at: 0 25 45 73 688 1101
Size: 25 20 28 615 413
Fragments arranged by size:
615 413 28 25 20
BanI G'GyrC_C
Cuts at: 0 163 1101
Size: 163 938
BanII G_rGCy'C
Cuts at: 0 26 37 84 1101
Size: 26 11 47 1017
Fragments arranged by size:
1017 47 26 11
BbeI G_GCGC'C
Cuts at: 0 167 1101
Size: 167 934
BbvCI CC'TCA_GC
Cuts at: 0 789 1101
Size: 789 312
BfrBI ATG'CAT
Cuts at: 0 638 1101
Size: 638 463
BglI GCCn_nnn'nGGC
Cuts at: 0 235 829 1101
Size: 235 594 272
Fragments arranged by size:
594 272 235
BglII A'GATC_T
Cuts at: 0 1001 1101
Size: 1001 100
Bme1580I G_kGCm'C
Cuts at: 0 938 1101
Size: 938 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1101
Size: 728 373
Bpu10I CC'TnA_GC
Cuts at: 0 789 1101
Size: 789 312
BsaHI Gr'CG_yC
Cuts at: 0 164 962 1101
Size: 164 798 139
Fragments arranged by size:
798 164 139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 402 417 420 429 432 435 846 849
Size: 402 15 3 9 3 3 411 3
Cuts at: 849 852 861 1101
Size: 3 9 240
Fragments arranged by size:
411 402 240 15 9 9 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 214 534 897 1072 1101
Size: 214 320 363 175 29
Fragments arranged by size:
363 320 214 175 29
BsiEI CG_ry'CG
Cuts at: 0 239 1101
Size: 239 862
BsmI GAATG_Cn'
Cuts at: 0 640 1084 1101
Size: 640 444 17
Fragments arranged by size:
640 444 17
Bsp1286I G_dGCh'C
Cuts at: 0 26 37 84 938 1101
Size: 26 11 47 854 163
Fragments arranged by size:
854 163 47 26 11
BspHI T'CATG_A
Cuts at: 0 758 1101
Size: 758 343
BsrBI CCG'CTC
Cuts at: 0 128 1101
Size: 128 973
BsrFI r'CCGG_y
Cuts at: 0 463 1101
Size: 463 638
BssHII G'CGCG_C
Cuts at: 0 190 244 1101
Size: 190 54 857
Fragments arranged by size:
857 190 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 974 1101
Size: 974 127
BstYI r'GATC_y
Cuts at: 0 1001 1101
Size: 1001 100
Bsu36I CC'TnA_GG
Cuts at: 0 873 1101
Size: 873 228
BtgI C'CryG_G
Cuts at: 0 233 1101
Size: 233 868
BtsI GCAGTG_nn'
Cuts at: 0 612 1101
Size: 612 489
EaeI y'GGCC_r
Cuts at: 0 179 236 281 1101
Size: 179 57 45 820
Fragments arranged by size:
820 179 57 45
EagI C'GGCC_G
Cuts at: 0 236 1101
Size: 236 865
EarI CTCTTCn'nnn_
Cuts at: 0 904 1019 1101
Size: 904 115 82
Fragments arranged by size:
904 115 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 386 405 423 902 1101
Size: 386 19 18 479 199
Fragments arranged by size:
479 386 199 19 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 728 1100 1101
Size: 686 42 372 1
Fragments arranged by size:
686 372 42 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 942 1101
Size: 942 159
EcoO109I rG'GnC_Cy
Cuts at: 0 42 1101
Size: 42 1059
HaeII r_GCGC'y
Cuts at: 0 123 167 224 662 1079 1101
Size: 123 44 57 438 417 22
Fragments arranged by size:
438 417 123 57 44 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 666 698 986 1018 1101
Size: 91 32 543 32 288 32 83
Fragments arranged by size:
543 288 91 83 32 32 32
KasI G'GCGC_C
Cuts at: 0 163 1101
Size: 163 938
MslI CAynn'nnrTG
Cuts at: 0 253 304 757 1089 1101
Size: 253 51 453 332 12
Fragments arranged by size:
453 332 253 51 12
MspA1I CmG'CkG
Cuts at: 0 235 320 1101
Size: 235 85 781
Fragments arranged by size:
781 235 85
NaeI GCC'GGC
Cuts at: 0 465 1101
Size: 465 636
NarI GG'CG_CC
Cuts at: 0 164 1101
Size: 164 937
NgoMIV G'CCGG_C
Cuts at: 0 463 1101
Size: 463 638
NotI GC'GGCC_GC
Cuts at: 0 236 1101
Size: 236 865
NsiI A_TGCA'T
Cuts at: 0 640 1101
Size: 640 461
NspI r_CATG'y
Cuts at: 0 258 510 1101
Size: 258 252 591
Fragments arranged by size:
591 258 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 311 534 1101
Size: 311 223 567
Fragments arranged by size:
567 311 223
PstI C_TGCA'G
Cuts at: 0 619 970 1101
Size: 619 351 131
Fragments arranged by size:
619 351 131
SacII CC_GC'GG
Cuts at: 0 236 1101
Size: 236 865
SapI GCTCTTCn'nnn_
Cuts at: 0 1019 1101
Size: 1019 82
SfcI C'TryA_G
Cuts at: 0 615 966 1101
Size: 615 351 135
Fragments arranged by size:
615 351 135
SfoI GGC'GCC
Cuts at: 0 165 1101
Size: 165 936
SgrAI Cr'CCGG_yG
Cuts at: 0 463 1101
Size: 463 638
SmaI CCC'GGG
Cuts at: 0 27 47 1101
Size: 27 20 1054
Fragments arranged by size:
1054 27 20
SphI G_CATG'C
Cuts at: 0 510 1101
Size: 510 591
SrfI GCCC'GGGC
Cuts at: 0 27 1101
Size: 27 1074
StyI C'CwwG_G
Cuts at: 0 56 1101
Size: 56 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 906 938 1101
Size: 906 32 163
Fragments arranged by size:
906 163 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1101
Size: 15 1086
XmaI C'CCGG_G
Cuts at: 0 25 45 1101
Size: 25 20 1056
Fragments arranged by size:
1056 25 20
Enzymes that do cut:
AcuI AfeI AloI AlwNI ApoI AvaI BanI BanII
BbeI BbvCI BfrBI BglI BglII Bme1580I BpmI Bpu10I
BsaHI BseRI BseYI BsiEI BsmI Bsp1286I BspHI BsrBI
BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MslI MspA1I NaeI NarI NgoMIV NotI NsiI
NspI PflMI PstI SacII SapI SfcI SfoI SgrAI
SmaI SphI SrfI StyI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsgI BsiHKAI BsiWI BsmBI BspEI BspMI
BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmnI ZraI