Restriction map for
pFN21AE2397
(Linear) (Six-base) MAPSORT of: KIEE2397.seq Check: 5541 from: 1 to: 2907
KIEE2397.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 332 2907
Size: 332 2575
AatII G_ACGT'C
Cuts at: 0 2033 2907
Size: 2033 874
AccI GT'mk_AC
Cuts at: 0 423 2907
Size: 423 2484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 2459 2907
Size: 1352 1107 448
Fragments arranged by size:
1352 1107 448
AleI CACnn'nnGTG
Cuts at: 0 1612 2907
Size: 1612 1295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1559 1883 2008 2083 2907
Size: 1559 324 125 75 824
Fragments arranged by size:
1559 824 324 125 75
AlwNI CAG_nnn'CTG
Cuts at: 0 985 2774 2907
Size: 985 1789 133
Fragments arranged by size:
1789 985 133
ApaLI G'TGCA_C
Cuts at: 0 514 2907
Size: 514 2393
ApoI r'AATT_y
Cuts at: 0 803 2474 2907
Size: 803 1671 433
Fragments arranged by size:
1671 803 433
AseI AT'TA_AT
Cuts at: 0 965 2907
Size: 965 1942
BamHI G'GATC_C
Cuts at: 0 51 2907
Size: 51 2856
BanI G'GyrC_C
Cuts at: 0 214 689 1138 2836 2907
Size: 214 475 449 1698 71
Fragments arranged by size:
1698 475 449 214 71
BanII G_rGCy'C
Cuts at: 0 1415 2756 2907
Size: 1415 1341 151
Fragments arranged by size:
1415 1341 151
BbeI G_GCGC'C
Cuts at: 0 693 2907
Size: 693 2214
BbsI GAAGACnn'nnnn_
Cuts at: 0 930 2695 2907
Size: 930 1765 212
Fragments arranged by size:
1765 930 212
BbvCI CC'TCA_GC
Cuts at: 0 628 1807 2907
Size: 628 1179 1100
Fragments arranged by size:
1179 1100 628
BciVI GTATCCnnnnn_n'
Cuts at: 0 2522 2907
Size: 2522 385
BclI T'GATC_A
Cuts at: 0 1772 2907
Size: 1772 1135
BfrBI ATG'CAT
Cuts at: 0 742 2907
Size: 742 2165
BglI GCCn_nnn'nGGC
Cuts at: 0 2023 2907
Size: 2023 884
Bme1580I G_kGCm'C
Cuts at: 0 518 1192 2907
Size: 518 674 1715
Fragments arranged by size:
1715 674 518
BmgBI CAC'GTC
Cuts at: 0 1575 2907
Size: 1575 1332
BmrI ACTGGGnnnn_n'
Cuts at: 0 2505 2677 2907
Size: 2505 172 230
Fragments arranged by size:
2505 230 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2576 2907
Size: 2576 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1169 1505 2140 2661 2907
Size: 812 357 336 635 521 246
Fragments arranged by size:
812 635 521 357 336 246
Bpu10I CC'TnA_GC
Cuts at: 0 628 1807 1825 2039 2907
Size: 628 1179 18 214 868
Fragments arranged by size:
1179 868 628 214 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1891 2907
Size: 1891 1016
BsaI GGTCTCn'nnnn_
Cuts at: 0 2598 2907
Size: 2598 309
BsaHI Gr'CG_yC
Cuts at: 0 690 713 1753 2030 2907
Size: 690 23 1040 277 877
Fragments arranged by size:
1040 877 690 277 23
BsaWI w'CCGG_w
Cuts at: 0 663 2907
Size: 663 2244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2035 2065 2907
Size: 2035 30 842
Fragments arranged by size:
2035 842 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1196 2907
Size: 1196 1711
BseYI C'CCAG_C
Cuts at: 0 564 1285 1414 1813 2020 2182 2907
Size: 564 721 129 399 207 162 725
Fragments arranged by size:
725 721 564 399 207 162 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1099 1636 2053 2907
Size: 354 745 537 417 854
Fragments arranged by size:
854 745 537 417 354
BsiHKAI G_wGCw'C
Cuts at: 0 518 2087 2756 2907
Size: 518 1569 669 151
Fragments arranged by size:
1569 669 518 151
BsmI GAATG_Cn'
Cuts at: 0 1965 2907
Size: 1965 942
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1565 2907
Size: 1565 1342
Bsp1286I G_dGCh'C
Cuts at: 0 518 1192 1415 2087 2756 2907
Size: 518 674 223 672 669 151
Fragments arranged by size:
674 672 669 518 223 151
BspEI T'CCGG_A
Cuts at: 0 663 2907
Size: 663 2244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 2340 2907
Size: 332 2008 567
Fragments arranged by size:
2008 567 332
BsrFI r'CCGG_y
Cuts at: 0 557 2907
Size: 557 2350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 502 621 2202 2875 2907
Size: 396 106 119 1581 673 32
Fragments arranged by size:
1581 673 396 119 106 32
BstYI r'GATC_y
Cuts at: 0 51 365 1683 2232 2907
Size: 51 314 1318 549 675
Fragments arranged by size:
1318 675 549 314 51
BstZ17I GTA'TAC
Cuts at: 0 424 2907
Size: 424 2483
Bsu36I CC'TnA_GG
Cuts at: 0 248 2907
Size: 248 2659
BtgI C'CryG_G
Cuts at: 0 198 701 1304 2907
Size: 198 503 603 1603
Fragments arranged by size:
1603 603 503 198
BtsI GCAGTG_nn'
Cuts at: 0 106 1607 1775 2065 2842 2907
Size: 106 1501 168 290 777 65
Fragments arranged by size:
1501 777 290 168 106 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 2907
Size: 400 2507
EaeI y'GGCC_r
Cuts at: 0 1589 2015 2336 2907
Size: 1589 426 321 571
Fragments arranged by size:
1589 571 426 321
EarI CTCTTCn'nnn_
Cuts at: 0 854 1469 2216 2907
Size: 854 615 747 691
Fragments arranged by size:
854 747 691 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 1169 2907
Size: 580 589 1738
Fragments arranged by size:
1738 589 580
EcoICRI GAG'CTC
Cuts at: 0 2754 2907
Size: 2754 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1169 1352 1505 2140 2459 2661 2907
Size: 812 357 183 153 635 319 202 246
Fragments arranged by size:
812 635 357 319 246 202 183 153
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2733 2894 2907
Size: 2733 161 13
Fragments arranged by size:
2733 161 13
EcoO109I rG'GnC_Cy
Cuts at: 0 1326 2625 2887 2907
Size: 1326 1299 262 20
Fragments arranged by size:
1326 1299 262 20
HaeII r_GCGC'y
Cuts at: 0 693 1602 2907
Size: 693 909 1305
Fragments arranged by size:
1305 909 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1074 1106 1300 1332 1725 1757 2035 2067
Size: 1074 32 194 32 393 32 278 32
Cuts at: 2067 2080 2112 2380 2412 2682 2714 2907
Size: 13 32 268 32 270 32 193
Fragments arranged by size:
1074 393 278 270 268 194 193 32
32 32 32 32 32 32 13
KasI G'GCGC_C
Cuts at: 0 689 2907
Size: 689 2218
MfeI C'AATT_G
Cuts at: 0 2654 2907
Size: 2654 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 2907
Size: 770 2137
MscI TGG'CCA
Cuts at: 0 2338 2907
Size: 2338 569
MslI CAynn'nnrTG
Cuts at: 0 326 1541 1612 2873 2907
Size: 326 1215 71 1261 34
Fragments arranged by size:
1261 1215 326 71 34
MspA1I CmG'CkG
Cuts at: 0 564 778 2020 2182 2345 2433 2457 2783
Size: 564 214 1242 162 163 88 24 326
Cuts at: 2783 2907
Size: 124
Fragments arranged by size:
1242 564 326 214 163 162 124 88
24
NarI GG'CG_CC
Cuts at: 0 690 2907
Size: 690 2217
NcoI C'CATG_G
Cuts at: 0 198 1304 2907
Size: 198 1106 1603
Fragments arranged by size:
1603 1106 198
NdeI CA'TA_TG
Cuts at: 0 2379 2907
Size: 2379 528
NsiI A_TGCA'T
Cuts at: 0 744 2907
Size: 744 2163
NspI r_CATG'y
Cuts at: 0 331 1164 1540 2907
Size: 331 833 376 1367
Fragments arranged by size:
1367 833 376 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 199 339 1228 2345 2907
Size: 199 140 889 1117 562
Fragments arranged by size:
1117 889 562 199 140
PfoI T'CCnGG_A
Cuts at: 0 501 1263 2117 2907
Size: 501 762 854 790
Fragments arranged by size:
854 790 762 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2310 2342 2907
Size: 2310 32 565
Fragments arranged by size:
2310 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 1326 2887 2907
Size: 1326 1561 20
Fragments arranged by size:
1561 1326 20
PstI C_TGCA'G
Cuts at: 0 608 2456 2907
Size: 608 1848 451
Fragments arranged by size:
1848 608 451
PvuII CAG'CTG
Cuts at: 0 564 778 2182 2345 2433 2457 2907
Size: 564 214 1404 163 88 24 450
Fragments arranged by size:
1404 564 450 214 163 88 24
SacI G_AGCT'C
Cuts at: 0 2756 2907
Size: 2756 151
SapI GCTCTTCn'nnn_
Cuts at: 0 2216 2907
Size: 2216 691
ScaI AGT'ACT
Cuts at: 0 2145 2907
Size: 2145 762
SexAI A'CCwGG_T
Cuts at: 0 210 2907
Size: 210 2697
SfcI C'TryA_G
Cuts at: 0 604 2452 2907
Size: 604 1848 455
Fragments arranged by size:
1848 604 455
SfoI GGC'GCC
Cuts at: 0 691 2907
Size: 691 2216
SmlI C'TyrA_G
Cuts at: 0 1906 2907
Size: 1906 1001
SpeI A'CTAG_T
Cuts at: 0 1024 2907
Size: 1024 1883
SphI G_CATG'C
Cuts at: 0 331 1540 2907
Size: 331 1209 1367
Fragments arranged by size:
1367 1209 331
SspI AAT'ATT
Cuts at: 0 74 140 969 2907
Size: 74 66 829 1938
Fragments arranged by size:
1938 829 74 66
StuI AGG'CCT
Cuts at: 0 30 2907
Size: 30 2877
StyI C'CwwG_G
Cuts at: 0 198 389 614 1304 2907
Size: 198 191 225 690 1603
Fragments arranged by size:
1603 690 225 198 191
TatI w'GTAC_w
Cuts at: 0 1198 2143 2907
Size: 1198 945 764
Fragments arranged by size:
1198 945 764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 2907
Size: 497 32 2378
Fragments arranged by size:
2378 497 32
Tth111I GACn'n_nGTC
Cuts at: 0 1636 2770 2907
Size: 1636 1134 137
Fragments arranged by size:
1636 1134 137
XbaI T'CTAG_A
Cuts at: 0 1506 2328 2892 2907
Size: 1506 822 564 15
Fragments arranged by size:
1506 822 564 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 2492 2574 2907
Size: 396 2096 82 333
Fragments arranged by size:
2096 396 333 82
XmnI GAAnn'nnTTC
Cuts at: 0 89 2907
Size: 89 2818
ZraI GAC'GTC
Cuts at: 0 2031 2907
Size: 2031 876
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApaLI
ApoI AseI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NsiI NspI PflMI PfoI PpiI PpuMI
PstI PvuII SacI SapI ScaI SexAI SfcI SfoI
SmlI SpeI SphI SspI StuI StyI TatI TstI
Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI AscI AsiSI AvaI AvrII BaeI BcgI BglII
BlpI BmtI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI
ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
NaeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI
SmaI SnaBI SrfI SwaI TaqII XhoI XmaI