(Linear) (Six-base) MAPSORT of: KIEE2396.seq  Check: 4008  from: 1  to: 774



KIEE2396.orf

 With 182 enzymes: SgfI * 

                        February 14, 2012 13:41  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535     774 
   Size:        535     239
 
AflIII A'CryG_T

Cuts at:      0     277     774 
   Size:        277     497
 
ApaLI G'TGCA_C

Cuts at:      0     624     774 
   Size:        624     150
 
ApoI r'AATT_y

Cuts at:      0     393     419     708     774 
   Size:        393      26     289      66

  Fragments arranged by size: 

                 393     289      66      26
 
BanII G_rGCy'C

Cuts at:      0     653     774 
   Size:        653     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      53     567     774 
   Size:         53     514     207

  Fragments arranged by size: 

                 514     207      53
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     551     774 
   Size:        551     223
 
BfrBI ATG'CAT

Cuts at:      0     292     774 
   Size:        292     482
 
Bme1580I G_kGCm'C

Cuts at:      0     628     774 
   Size:        628     146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     774 
   Size:        372     402
 
BseYI C'CCAG_C

Cuts at:      0     597     774 
   Size:        597     177
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     774 
   Size:        107     667
 
BsiHKAI G_wGCw'C

Cuts at:      0     628     774 
   Size:        628     146
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     735     774 
   Size:        735      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     628     653     774 
   Size:        628      25     121

  Fragments arranged by size: 

                 628     121      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      98     339     774 
   Size:         98     241     435

  Fragments arranged by size: 

                 435     241      98
 
BstBI TT'CG_AA

Cuts at:      0     664     774 
   Size:        664     110
 
BtsI GCAGTG_nn'

Cuts at:      0     154     608     774 
   Size:        154     454     166

  Fragments arranged by size: 

                 454     166     154
 
EaeI y'GGCC_r

Cuts at:      0       2     774 
   Size:          2     772
 
EarI CTCTTCn'nnn_

Cuts at:      0     669     774 
   Size:        669     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     535     774 
   Size:        372     163     239

  Fragments arranged by size: 

                 372     239     163
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681     774 
   Size:        681      93
 
EcoRI G'AATT_C

Cuts at:      0     708     774 
   Size:        708      66
 
EcoRV GAT'ATC

Cuts at:      0     240     522     774 
   Size:        240     282     252

  Fragments arranged by size: 

                 282     252     240
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      58     322     774 
   Size:         58     264     452

  Fragments arranged by size: 

                 452     264      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     774 
   Size:        370      32     372

  Fragments arranged by size: 

                 372     370      32
 
MscI TGG'CCA

Cuts at:      0       4     774 
   Size:          4     770
 
MspA1I CmG'CkG

Cuts at:      0     252     774 
   Size:        252     522
 
NsiI A_TGCA'T

Cuts at:      0     294     774 
   Size:        294     480
 
NspI r_CATG'y

Cuts at:      0     281     774 
   Size:        281     493
 
PciI A'CATG_T

Cuts at:      0     277     774 
   Size:        277     497
 
PfoI T'CCnGG_A

Cuts at:      0     444     774 
   Size:        444     330
 
PpuMI rG'GwC_Cy

Cuts at:      0     681     774 
   Size:        681      93
 
PvuII CAG'CTG

Cuts at:      0     252     774 
   Size:        252     522
 
SanDI GG'GwC_CC

Cuts at:      0     681     774 
   Size:        681      93
 
SfcI C'TryA_G

Cuts at:      0     247     774 
   Size:        247     527
 
StyI C'CwwG_G

Cuts at:      0       5     774 
   Size:          5     769
 
XmnI GAAnn'nnTTC

Cuts at:      0     461     564     774 
   Size:        461     103     210

  Fragments arranged by size: 

                 461     210     103
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI    BanII     BbsI    BciVI    BfrBI
 Bme1580I     BpmI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BstBI     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I     MscI   MspA1I     NsiI     NspI     PciI     PfoI
    PpuMI    PvuII    SanDI     SfcI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI