Restriction map for
pFN21AE2388
(Linear) (Six-base) MAPSORT of: KIEE2388.seq Check: 9123 from: 1 to: 4452
KIEE2388.orf
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2664 4452
Size: 2664 1788
AccI GT'mk_AC
Cuts at: 0 1150 1558 2978 4452
Size: 1150 408 1420 1474
Fragments arranged by size:
1474 1420 1150 408
AclI AA'CG_TT
Cuts at: 0 3316 4452
Size: 3316 1136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 2951 4452
Size: 175 2776 1501
Fragments arranged by size:
2776 1501 175
AhdI GACnn_n'nnGTC
Cuts at: 0 927 1157 2509 4452
Size: 927 230 1352 1943
Fragments arranged by size:
1943 1352 927 230
AleI CACnn'nnGTG
Cuts at: 0 337 4452
Size: 337 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3334 4452
Size: 3334 1118
AlwNI CAG_nnn'CTG
Cuts at: 0 321 1990 2836 3856 4452
Size: 321 1669 846 1020 596
Fragments arranged by size:
1669 1020 846 596 321
ApaI G_GGCC'C
Cuts at: 0 2170 2422 3067 3490 4452
Size: 2170 252 645 423 962
Fragments arranged by size:
2170 962 645 423 252
ApoI r'AATT_y
Cuts at: 0 666 1492 1645 2318 3441 3943 4452
Size: 666 826 153 673 1123 502 509
Fragments arranged by size:
1123 826 673 666 509 502 153
AvaI C'yCGr_G
Cuts at: 0 2032 2492 4452
Size: 2032 460 1960
Fragments arranged by size:
2032 1960 460
AvrII C'CTAG_G
Cuts at: 0 907 4452
Size: 907 3545
BamHI G'GATC_C
Cuts at: 0 2179 4452
Size: 2179 2273
BanI G'GyrC_C
Cuts at: 0 1923 2877 4410 4452
Size: 1923 954 1533 42
Fragments arranged by size:
1923 1533 954 42
BanII G_rGCy'C
Cuts at: 0 41 275 1232 2170 2283 2422 3040 3067
Size: 41 234 957 938 113 139 618 27
Cuts at: 3067 3074 3490 3991 4452
Size: 7 416 501 461
Fragments arranged by size:
957 938 618 501 461 416 234 139
113 41 27 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 452 612 1516 4452
Size: 381 71 160 904 2936
Fragments arranged by size:
2936 904 381 160 71
BbvCI CC'TCA_GC
Cuts at: 0 4112 4452
Size: 4112 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 840 874 1130 1164 2570 2604 4452
Size: 840 34 256 34 1406 34 1848
Fragments arranged by size:
1848 1406 840 256 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 3308 4452
Size: 3308 1144
BglII A'GATC_T
Cuts at: 0 845 4452
Size: 845 3607
Bme1580I G_kGCm'C
Cuts at: 0 7 462 1867 2170 2422 2880 3067 3490
Size: 7 455 1405 303 252 458 187 423
Cuts at: 3490 4452
Size: 962
Fragments arranged by size:
1405 962 458 455 423 303 252 187
7
BmgBI CAC'GTC
Cuts at: 0 156 1344 4452
Size: 156 1188 3108
Fragments arranged by size:
3108 1188 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 2630 4452
Size: 2630 1822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 807 4452
Size: 60 747 3645
Fragments arranged by size:
3645 747 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1152 1220 1640 1713 2033 2060 2219 2267
Size: 1152 68 420 73 320 27 159 48
Cuts at: 2267 2606 3590 4452
Size: 339 984 862
Fragments arranged by size:
1152 984 862 420 339 320 159 73
68 48 27
Bpu10I CC'TnA_GC
Cuts at: 0 1189 4112 4452
Size: 1189 2923 340
Fragments arranged by size:
2923 1189 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1202 4452
Size: 555 647 3250
Fragments arranged by size:
3250 647 555
BsaI GGTCTCn'nnnn_
Cuts at: 0 2496 3176 3693 4452
Size: 2496 680 517 759
Fragments arranged by size:
2496 759 680 517
BsaAI yAC'GTr
Cuts at: 0 2481 4452
Size: 2481 1971
BsaWI w'CCGG_w
Cuts at: 0 2880 4452
Size: 2880 1572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 628 658 3380 3410 4083 4113 4452
Size: 628 30 2722 30 673 30 339
Fragments arranged by size:
2722 673 628 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 139 649 811 967 1012 1099 1194
Size: 25 114 510 162 156 45 87 95
Cuts at: 1194 1998 2137 2724 3075 4013 4420 4452
Size: 804 139 587 351 938 407 32
Fragments arranged by size:
938 804 587 510 407 351 162 156
139 114 95 87 45 32 25
BseYI C'CCAG_C
Cuts at: 0 30 266 1693 1915 2326 3159 3364 3510
Size: 30 236 1427 222 411 833 205 146
Cuts at: 3510 3524 3735 4193 4452
Size: 14 211 458 259
Fragments arranged by size:
1427 833 458 411 259 236 222 211
205 146 30 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1051 1134 2065 2698 2765 3009 4452
Size: 334 717 83 931 633 67 244 1443
Fragments arranged by size:
1443 931 717 633 334 244 83 67
BsiEI CG_ry'CG
Cuts at: 0 26 2344 2584 4120 4452
Size: 26 2318 240 1536 332
Fragments arranged by size:
2318 1536 332 240 26
BsiHKAI G_wGCw'C
Cuts at: 0 41 275 1232 2655 3040 3074 3991 4452
Size: 41 234 957 1423 385 34 917 461
Fragments arranged by size:
1423 957 917 461 385 234 41 34
BsmI GAATG_Cn'
Cuts at: 0 3786 3822 4343 4452
Size: 3786 36 521 109
Fragments arranged by size:
3786 521 109 36
Bsp1286I G_dGCh'C
Cuts at: 0 7 41 275 462 1232 1867 2170 2283
Size: 7 34 234 187 770 635 303 113
Cuts at: 2283 2422 2655 2880 3040 3067 3074 3490 3991
Size: 139 233 225 160 27 7 416 501
Cuts at: 3991 4452
Size: 461
Fragments arranged by size:
770 635 501 461 416 303 234 233
225 187 160 139 113 34 27 7
7
BspHI T'CATG_A
Cuts at: 0 2498 4452
Size: 2498 1954
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 2664 2802 4452
Size: 329 2335 138 1650
Fragments arranged by size:
2335 1650 329 138
BsrBI CCG'CTC
Cuts at: 0 44 139 1727 4452
Size: 44 95 1588 2725
Fragments arranged by size:
2725 1588 95 44
BsrDI GCAATG_nn'
Cuts at: 0 1607 3119 4452
Size: 1607 1512 1333
Fragments arranged by size:
1607 1512 1333
BsrFI r'CCGG_y
Cuts at: 0 368 4119 4452
Size: 368 3751 333
Fragments arranged by size:
3751 368 333
BsrGI T'GTAC_A
Cuts at: 0 491 4322 4452
Size: 491 3831 130
Fragments arranged by size:
3831 491 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3856 4452
Size: 3856 596
BstEII G'GTnAC_C
Cuts at: 0 765 4452
Size: 765 3687
BstYI r'GATC_y
Cuts at: 0 344 845 1986 2179 4452
Size: 344 501 1141 193 2273
Fragments arranged by size:
2273 1141 501 344 193
BstZ17I GTA'TAC
Cuts at: 0 1559 4452
Size: 1559 2893
Bsu36I CC'TnA_GG
Cuts at: 0 2568 4452
Size: 2568 1884
BtgI C'CryG_G
Cuts at: 0 1433 1766 1852 2391 3191 3785 4452
Size: 1433 333 86 539 800 594 667
Fragments arranged by size:
1433 800 667 594 539 333 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 51 2734 4452
Size: 51 2683 1718
Fragments arranged by size:
2683 1718 51
BtsI GCAGTG_nn'
Cuts at: 0 1034 1106 1122 1319 3285 3857 4452
Size: 1034 72 16 197 1966 572 595
Fragments arranged by size:
1966 1034 595 572 197 72 16
ClaI AT'CG_AT
Cuts at: 0 2340 4452
Size: 2340 2112
DraI TTT'AAA
Cuts at: 0 1934 4452
Size: 1934 2518
DraIII CAC_nnn'GTG
Cuts at: 0 2956 4080 4452
Size: 2956 1124 372
Fragments arranged by size:
2956 1124 372
EaeI y'GGCC_r
Cuts at: 0 23 62 1875 2290 3788 4117 4121 4452
Size: 23 39 1813 415 1498 329 4 331
Fragments arranged by size:
1813 1498 415 331 329 39 23 4
EagI C'GGCC_G
Cuts at: 0 23 4117 4452
Size: 23 4094 335
Fragments arranged by size:
4094 335 23
EarI CTCTTCn'nnn_
Cuts at: 0 146 204 332 594 743 1001 1058 1171
Size: 146 58 128 262 149 258 57 113
Cuts at: 1171 1818 2672 2936 3772 3893 4155 4452
Size: 647 854 264 836 121 262 297
Fragments arranged by size:
854 836 647 297 264 262 262 258
149 146 128 121 113 58 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 4016 4452
Size: 91 3925 436
Fragments arranged by size:
3925 436 91
EcoICRI GAG'CTC
Cuts at: 0 39 273 1230 3038 3072 3989 4452
Size: 39 234 957 1808 34 917 463
Fragments arranged by size:
1808 957 917 463 234 39 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1152 1220 1640 1713 2033 2060 2219
Size: 175 977 68 420 73 320 27 159
Cuts at: 2219 2267 2606 2951 3590 4452
Size: 48 339 345 639 862
Fragments arranged by size:
977 862 639 420 345 339 320 175
159 73 68 48 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 826 1976 2567 4210 4452
Size: 190 636 1150 591 1643 242
Fragments arranged by size:
1643 1150 636 591 242 190
EcoO109I rG'GnC_Cy
Cuts at: 0 57 318 405 501 701 788 1428 1734
Size: 57 261 87 96 200 87 640 306
Cuts at: 1734 2101 2410 2449 3267 3487 3761 4096 4219
Size: 367 309 39 818 220 274 335 123
Cuts at: 4219 4306 4452
Size: 87 146
Fragments arranged by size:
818 640 367 335 309 306 274 261
220 200 146 123 96 87 87 87
57 39
EcoRI G'AATT_C
Cuts at: 0 3441 4452
Size: 3441 1011
EcoRV GAT'ATC
Cuts at: 0 216 4452
Size: 216 4236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 157 4325 4452
Size: 157 4168 127
Fragments arranged by size:
4168 157 127
FseI GG_CCGG'CC
Cuts at: 0 4123 4452
Size: 4123 329
HaeII r_GCGC'y
Cuts at: 0 1810 3212 4452
Size: 1810 1402 1240
Fragments arranged by size:
1810 1402 1240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 913 945 1143 1175 2495 2527 4083 4115
Size: 913 32 198 32 1320 32 1556 32
Cuts at: 4115 4452
Size: 337
Fragments arranged by size:
1556 1320 913 337 198 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1151 4452
Size: 1151 3301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1286 2061 3859 4452
Size: 1061 225 775 1798 593
Fragments arranged by size:
1798 1061 775 593 225
MscI TGG'CCA
Cuts at: 0 3790 4452
Size: 3790 662
MslI CAynn'nnrTG
Cuts at: 0 149 337 1324 1904 4452
Size: 149 188 987 580 2548
Fragments arranged by size:
2548 987 580 188 149
MspA1I CmG'CkG
Cuts at: 0 266 1854 3414 3448 4116 4452
Size: 266 1588 1560 34 668 336
Fragments arranged by size:
1588 1560 668 336 266 34
NaeI GCC'GGC
Cuts at: 0 4121 4452
Size: 4121 331
NcoI C'CATG_G
Cuts at: 0 1433 1766 2391 3785 4452
Size: 1433 333 625 1394 667
Fragments arranged by size:
1433 1394 667 625 333
NdeI CA'TA_TG
Cuts at: 0 2333 4452
Size: 2333 2119
NgoMIV G'CCGG_C
Cuts at: 0 4119 4452
Size: 4119 333
NruI TCG'CGA
Cuts at: 0 782 4452
Size: 782 3670
NspI r_CATG'y
Cuts at: 0 1145 2229 2580 4452
Size: 1145 1084 351 1872
Fragments arranged by size:
1872 1145 1084 351
PasI CC'CwG_GG
Cuts at: 0 2859 2869 3714 4139 4183 4452
Size: 2859 10 845 425 44 269
Fragments arranged by size:
2859 845 425 269 44 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 2176 2525 2959 3568 4452
Size: 2176 349 434 609 884
Fragments arranged by size:
2176 884 609 434 349
PfoI T'CCnGG_A
Cuts at: 0 314 579 1617 2018 4313 4452
Size: 314 265 1038 401 2295 139
Fragments arranged by size:
2295 1038 401 314 265 139
PmlI CAC'GTG
Cuts at: 0 2481 4452
Size: 2481 1971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 794 826 4452
Size: 794 32 3626
Fragments arranged by size:
3626 794 32
PpuMI rG'GwC_Cy
Cuts at: 0 57 318 501 701 788 1734 2101 2410
Size: 57 261 183 200 87 946 367 309
Cuts at: 2410 2449 3761 4219 4306 4452
Size: 39 1312 458 87 146
Fragments arranged by size:
1312 946 458 367 309 261 200 183
146 87 87 57 39
PshAI GACnn'nnGTC
Cuts at: 0 699 4452
Size: 699 3753
PspOMI G'GGCC_C
Cuts at: 0 2166 2418 3063 3486 4452
Size: 2166 252 645 423 966
Fragments arranged by size:
2166 966 645 423 252
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3267 3299 4452
Size: 3267 32 1153
Fragments arranged by size:
3267 1153 32
PstI C_TGCA'G
Cuts at: 0 632 3017 3479 4091 4452
Size: 632 2385 462 612 361
Fragments arranged by size:
2385 632 612 462 361
PvuI CG_AT'CG
Cuts at: 0 2344 4452
Size: 2344 2108
PvuII CAG'CTG
Cuts at: 0 3448 4452
Size: 3448 1004
SacI G_AGCT'C
Cuts at: 0 41 275 1232 3040 3074 3991 4452
Size: 41 234 957 1808 34 917 461
Fragments arranged by size:
1808 957 917 461 234 41 34
SacII CC_GC'GG
Cuts at: 0 1855 4452
Size: 1855 2597
SalI G'TCGA_C
Cuts at: 0 1149 4452
Size: 1149 3303
SanDI GG'GwC_CC
Cuts at: 0 701 1734 4219 4452
Size: 701 1033 2485 233
Fragments arranged by size:
2485 1033 701 233
SapI GCTCTTCn'nnn_
Cuts at: 0 146 1001 2672 4452
Size: 146 855 1671 1780
Fragments arranged by size:
1780 1671 855 146
SbfI CC_TGCA'GG
Cuts at: 0 3479 4452
Size: 3479 973
SexAI A'CCwGG_T
Cuts at: 0 2952 4452
Size: 2952 1500
SfcI C'TryA_G
Cuts at: 0 628 1163 3013 3475 4087 4452
Size: 628 535 1850 462 612 365
Fragments arranged by size:
1850 628 612 535 462 365
SmaI CCC'GGG
Cuts at: 0 2034 4452
Size: 2034 2418
SmlI C'TyrA_G
Cuts at: 0 570 1217 4452
Size: 570 647 3235
Fragments arranged by size:
3235 647 570
StuI AGG'CCT
Cuts at: 0 3473 4247 4366 4452
Size: 3473 774 119 86
Fragments arranged by size:
3473 774 119 86
StyI C'CwwG_G
Cuts at: 0 671 705 717 907 1433 1484 1766 2391
Size: 671 34 12 190 526 51 282 625
Cuts at: 2391 2406 2414 3168 3270 3538 3757 3785 4452
Size: 15 8 754 102 268 219 28 667
Fragments arranged by size:
754 671 667 625 526 282 268 219
190 102 51 34 28 15 12 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 1929 2464 2649 2837 3575 3635 4196
Size: 430 1499 535 185 188 738 60 561
Cuts at: 4196 4207 4452
Size: 11 245
Fragments arranged by size:
1499 738 561 535 430 245 188 185
60 11
TatI w'GTAC_w
Cuts at: 0 491 2364 4322 4452
Size: 491 1873 1958 130
Fragments arranged by size:
1958 1873 491 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1348 1380 2164 2196 2379 2411
Size: 1096 32 220 32 784 32 183 32
Cuts at: 2411 3380 3412 4452
Size: 969 32 1040
Fragments arranged by size:
1096 1040 969 784 220 183 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 385 1685 4452
Size: 385 1300 2767
Fragments arranged by size:
2767 1300 385
XbaI T'CTAG_A
Cuts at: 0 1338 4452
Size: 1338 3114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2856 3565 4452
Size: 2856 709 887
Fragments arranged by size:
2856 887 709
XmaI C'CCGG_G
Cuts at: 0 2032 4452
Size: 2032 2420
Enzymes that do cut:
AarI AccI AclI AcuI AhdI AleI AlfI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI HaeII Hin4I HincII MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NruI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AloI ApaLI
AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI
BlpI BmtI BsaBI BsaHI BsiWI BsmBI BspEI BssHII
BssSI BstBI BstXI CspCI DrdI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NarI NheI NotI
NsiI PacI PciI PmeI PsiI PspXI RsrII ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XhoI XmnI ZraI