Restriction map for
pFN21AE2377
(Linear) (Six-base) MAPSORT of: KIEE2377.seq Check: 4973 from: 1 to: 4170
KIEE2377.orf
With 182 enzymes: SgfI *
October 27, 2011 13:18 ..
AccI GT'mk_AC
Cuts at: 0 585 4170
Size: 585 3585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 563 755 1118 1247 1952 2534 4140
Size: 236 327 192 363 129 705 582 1606
Cuts at: 4140 4170
Size: 30
Fragments arranged by size:
1606 705 582 363 327 236 192 129
30
AflII C'TTAA_G
Cuts at: 0 2976 4170
Size: 2976 1194
AflIII A'CryG_T
Cuts at: 0 211 2521 2588 4170
Size: 211 2310 67 1582
Fragments arranged by size:
2310 1582 211 67
AleI CACnn'nnGTG
Cuts at: 0 1070 2110 4170
Size: 1070 1040 2060
Fragments arranged by size:
2060 1070 1040
AlwNI CAG_nnn'CTG
Cuts at: 0 2100 4170
Size: 2100 2070
ApoI r'AATT_y
Cuts at: 0 246 1170 1360 1367 1777 2182 2791 3308
Size: 246 924 190 7 410 405 609 517
Cuts at: 3308 3387 3494 4059 4170
Size: 79 107 565 111
Fragments arranged by size:
924 609 565 517 410 405 246 190
111 107 79 7
AseI AT'TA_AT
Cuts at: 0 2015 4170
Size: 2015 2155
AvaI C'yCGr_G
Cuts at: 0 3890 4170
Size: 3890 280
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3109 3142 4170
Size: 3109 33 1028
Fragments arranged by size:
3109 1028 33
BanI G'GyrC_C
Cuts at: 0 1157 2427 3523 4170
Size: 1157 1270 1096 647
Fragments arranged by size:
1270 1157 1096 647
BanII G_rGCy'C
Cuts at: 0 61 4170
Size: 61 4109
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 614 752 3290 3653 3764 3775 4018
Size: 374 240 138 2538 363 111 11 243
Cuts at: 4018 4170
Size: 152
Fragments arranged by size:
2538 374 363 243 240 152 138 111
11
BciVI GTATCCnnnnn_n'
Cuts at: 0 1891 4170
Size: 1891 2279
BclI T'GATC_A
Cuts at: 0 1551 4170
Size: 1551 2619
BglI GCCn_nnn'nGGC
Cuts at: 0 130 4170
Size: 130 4040
BglII A'GATC_T
Cuts at: 0 223 1970 4170
Size: 223 1747 2200
Fragments arranged by size:
2200 1747 223
BlpI GC'TnA_GC
Cuts at: 0 2825 4170
Size: 2825 1345
Bme1580I G_kGCm'C
Cuts at: 0 2529 4170
Size: 2529 1641
BmrI ACTGGGnnnn_n'
Cuts at: 0 2872 4170
Size: 2872 1298
BmtI G_CTAG'C
Cuts at: 0 1669 1882 4170
Size: 1669 213 2288
Fragments arranged by size:
2288 1669 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1798 4170
Size: 1798 2372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1595 2648 3227 4170
Size: 112 1483 1053 579 943
Fragments arranged by size:
1483 1053 943 579 112
Bpu10I CC'TnA_GC
Cuts at: 0 2027 2894 4170
Size: 2027 867 1276
Fragments arranged by size:
2027 1276 867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3599 4170
Size: 3599 571
BsaI GGTCTCn'nnnn_
Cuts at: 0 3634 4170
Size: 3634 536
BsaAI yAC'GTr
Cuts at: 0 214 2589 4170
Size: 214 2375 1581
Fragments arranged by size:
2375 1581 214
BsaBI GATnn'nnATC
Cuts at: 0 1241 1844 3158 3200 4170
Size: 1241 603 1314 42 970
Fragments arranged by size:
1314 1241 970 603 42
BsaWI w'CCGG_w
Cuts at: 0 206 2769 3643 4170
Size: 206 2563 874 527
Fragments arranged by size:
2563 874 527 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 4170
Size: 22 30 4118
Fragments arranged by size:
4118 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1083 3333 4170
Size: 1083 2250 837
Fragments arranged by size:
2250 1083 837
BseYI C'CCAG_C
Cuts at: 0 972 4170
Size: 972 3198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1470 4170
Size: 13 1457 2700
Fragments arranged by size:
2700 1457 13
BsiEI CG_ry'CG
Cuts at: 0 105 125 4170
Size: 105 20 4045
Fragments arranged by size:
4045 105 20
BsiHKAI G_wGCw'C
Cuts at: 0 98 362 1437 4170
Size: 98 264 1075 2733
Fragments arranged by size:
2733 1075 264 98
BsmI GAATG_Cn'
Cuts at: 0 3579 4170
Size: 3579 591
Bsp1286I G_dGCh'C
Cuts at: 0 61 98 362 1437 2529 4170
Size: 61 37 264 1075 1092 1641
Fragments arranged by size:
1641 1092 1075 264 61 37
BspHI T'CATG_A
Cuts at: 0 1481 3987 4170
Size: 1481 2506 183
Fragments arranged by size:
2506 1481 183
BsrBI CCG'CTC
Cuts at: 0 119 4170
Size: 119 4051
BsrFI r'CCGG_y
Cuts at: 0 124 4170
Size: 124 4046
BsrGI T'GTAC_A
Cuts at: 0 653 3781 4170
Size: 653 3128 389
Fragments arranged by size:
3128 653 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1231 4170
Size: 1231 2939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1857 4170
Size: 1857 2313
BstYI r'GATC_y
Cuts at: 0 223 1740 1970 2001 3201 4170
Size: 223 1517 230 31 1200 969
Fragments arranged by size:
1517 1200 969 230 223 31
BtgI C'CryG_G
Cuts at: 0 41 53 146 4170
Size: 41 12 93 4024
Fragments arranged by size:
4024 93 41 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 185 4081 4170
Size: 185 3896 89
Fragments arranged by size:
3896 185 89
BtsI GCAGTG_nn'
Cuts at: 0 536 726 4170
Size: 536 190 3444
Fragments arranged by size:
3444 536 190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3381 3416 4170
Size: 3381 35 754
Fragments arranged by size:
3381 754 35
DraI TTT'AAA
Cuts at: 0 1365 4170
Size: 1365 2805
DrdI GACnn_nn'nnGTC
Cuts at: 0 1022 4170
Size: 1022 3148
EaeI y'GGCC_r
Cuts at: 0 50 102 122 326 4170
Size: 50 52 20 204 3844
Fragments arranged by size:
3844 204 52 50 20
EagI C'GGCC_G
Cuts at: 0 102 122 4170
Size: 102 20 4048
Fragments arranged by size:
4048 102 20
EarI CTCTTCn'nnn_
Cuts at: 0 350 731 857 1868 2013 2708 3481 4170
Size: 350 381 126 1011 145 695 773 689
Fragments arranged by size:
1011 773 695 689 381 350 145 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1137 4170
Size: 1137 3033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 236 563 755 1118 1247 1595 1952
Size: 112 124 327 192 363 129 348 357
Cuts at: 1952 2534 2648 3227 4140 4170
Size: 582 114 579 913 30
Fragments arranged by size:
913 582 579 363 357 348 327 192
129 124 114 112 30
EcoRI G'AATT_C
Cuts at: 0 246 1777 2182 4170
Size: 246 1531 405 1988
Fragments arranged by size:
1988 1531 405 246
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 568 1043 3292 4170
Size: 568 475 2249 878
Fragments arranged by size:
2249 878 568 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 468 500 1903 1935 2251 2283
Size: 163 32 273 32 1403 32 316 32
Cuts at: 2283 2887 2919 3079 3111 3397 3429 4102 4134
Size: 604 32 160 32 286 32 673 32
Cuts at: 4134 4170
Size: 36
Fragments arranged by size:
1403 673 604 316 286 273 163 160
36 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2139 3039 4170
Size: 2139 900 1131
Fragments arranged by size:
2139 1131 900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1875 1979 2586 4034 4170
Size: 1875 104 607 1448 136
Fragments arranged by size:
1875 1448 607 136 104
MscI TGG'CCA
Cuts at: 0 328 4170
Size: 328 3842
MslI CAynn'nnrTG
Cuts at: 0 434 761 1070 2110 4170
Size: 434 327 309 1040 2060
Fragments arranged by size:
2060 1040 434 327 309
MspA1I CmG'CkG
Cuts at: 0 43 148 2434 4170
Size: 43 105 2286 1736
Fragments arranged by size:
2286 1736 105 43
NaeI GCC'GGC
Cuts at: 0 126 4170
Size: 126 4044
NgoMIV G'CCGG_C
Cuts at: 0 124 4170
Size: 124 4046
NheI G'CTAG_C
Cuts at: 0 1665 1878 4170
Size: 1665 213 2292
Fragments arranged by size:
2292 1665 213
NspI r_CATG'y
Cuts at: 0 1060 2179 2525 3782 4170
Size: 1060 1119 346 1257 388
Fragments arranged by size:
1257 1119 1060 388 346
PciI A'CATG_T
Cuts at: 0 2521 4170
Size: 2521 1649
PflMI CCAn_nnn'nTGG
Cuts at: 0 1841 2407 4170
Size: 1841 566 1763
Fragments arranged by size:
1841 1763 566
PfoI T'CCnGG_A
Cuts at: 0 3204 4170
Size: 3204 966
PmlI CAC'GTG
Cuts at: 0 214 2589 4170
Size: 214 2375 1581
Fragments arranged by size:
2375 1581 214
PsiI TTA'TAA
Cuts at: 0 529 1202 4170
Size: 529 673 2968
Fragments arranged by size:
2968 673 529
PspXI vC'TCGA_Gb
Cuts at: 0 3890 4170
Size: 3890 280
PstI C_TGCA'G
Cuts at: 0 1997 2162 4170
Size: 1997 165 2008
Fragments arranged by size:
2008 1997 165
PvuII CAG'CTG
Cuts at: 0 2434 4170
Size: 2434 1736
SacII CC_GC'GG
Cuts at: 0 44 149 4170
Size: 44 105 4021
Fragments arranged by size:
4021 105 44
SapI GCTCTTCn'nnn_
Cuts at: 0 350 1868 4170
Size: 350 1518 2302
Fragments arranged by size:
2302 1518 350
ScaI AGT'ACT
Cuts at: 0 2502 3453 4170
Size: 2502 951 717
Fragments arranged by size:
2502 951 717
SfcI C'TryA_G
Cuts at: 0 1736 1993 2158 3168 3773 4170
Size: 1736 257 165 1010 605 397
Fragments arranged by size:
1736 1010 605 397 257 165
SmlI C'TyrA_G
Cuts at: 0 2976 3614 3890 4170
Size: 2976 638 276 280
Fragments arranged by size:
2976 638 280 276
SpeI A'CTAG_T
Cuts at: 0 1981 4170
Size: 1981 2189
SphI G_CATG'C
Cuts at: 0 1060 4170
Size: 1060 3110
SspI AAT'ATT
Cuts at: 0 2384 2649 4170
Size: 2384 265 1521
Fragments arranged by size:
2384 1521 265
StuI AGG'CCT
Cuts at: 0 1558 1758 4170
Size: 1558 200 2412
Fragments arranged by size:
2412 1558 200
StyI C'CwwG_G
Cuts at: 0 62 4170
Size: 62 4108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 2625 4170
Size: 380 2245 1545
Fragments arranged by size:
2245 1545 380
TatI w'GTAC_w
Cuts at: 0 421 549 653 2500 3451 3781 4170
Size: 421 128 104 1847 951 330 389
Fragments arranged by size:
1847 951 421 389 330 128 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 4170
Size: 22 32 4116
Fragments arranged by size:
4116 32 22
XbaI T'CTAG_A
Cuts at: 0 3319 4170
Size: 3319 851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 4170
Size: 60 4110
XhoI C'TCGA_G
Cuts at: 0 3890 4170
Size: 3890 280
XmnI GAAnn'nnTTC
Cuts at: 0 3308 3723 4170
Size: 3308 415 447
Fragments arranged by size:
3308 447 415
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApoI AseI
AvaI BaeI BanI BanII BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI
BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI Hin4I HincII MmeI MscI MslI MspA1I NaeI
NgoMIV NheI NspI PciI PflMI PfoI PmlI PsiI
PspXI PstI PvuII SacII SapI ScaI SfcI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI
BbvCI BcgI BfrBI BmgBI BsaHI BsiWI BsmBI BspEI
BspMI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NotI NruI NsiI PacI PasI
PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI RsrII
SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SwaI Tth111I XmaI ZraI