Restriction map for
pFN21AE2376
(Linear) (Six-base) MAPSORT of: KIEE2376.seq Check: 3474 from: 1 to: 1551
KIEE2376.orf
With 182 enzymes: SgfI *
September 20, 2011 13:23 ..
AccI GT'mk_AC
Cuts at: 0 695 1551
Size: 695 856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1041 1379 1551
Size: 1041 338 172
Fragments arranged by size:
1041 338 172
AflIII A'CryG_T
Cuts at: 0 855 1551
Size: 855 696
AvaI C'yCGr_G
Cuts at: 0 72 106 259 1551
Size: 72 34 153 1292
Fragments arranged by size:
1292 153 72 34
BanI G'GyrC_C
Cuts at: 0 427 1551
Size: 427 1124
BanII G_rGCy'C
Cuts at: 0 41 55 104 259 424 482 1551
Size: 41 14 49 155 165 58 1069
Fragments arranged by size:
1069 165 155 58 49 41 14
BbeI G_GCGC'C
Cuts at: 0 431 1551
Size: 431 1120
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 1367 1551
Size: 296 1071 184
Fragments arranged by size:
1071 296 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1214 1248 1551
Size: 1214 34 303
Fragments arranged by size:
1214 303 34
BglI GCCn_nnn'nGGC
Cuts at: 0 274 377 506 611 1551
Size: 274 103 129 105 940
Fragments arranged by size:
940 274 129 105 103
BglII A'GATC_T
Cuts at: 0 828 1551
Size: 828 723
BlpI GC'TnA_GC
Cuts at: 0 98 1551
Size: 98 1453
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 1551
Size: 581 970
BmtI G_CTAG'C
Cuts at: 0 1036 1551
Size: 1036 515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 429 1551
Size: 57 372 1122
Fragments arranged by size:
1122 372 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 493 528 710 1551
Size: 52 441 35 182 841
Fragments arranged by size:
841 441 182 52 35
Bpu10I CC'TnA_GC
Cuts at: 0 49 646 1551
Size: 49 597 905
Fragments arranged by size:
905 597 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 1551
Size: 400 1151
BsaHI Gr'CG_yC
Cuts at: 0 428 1551
Size: 428 1123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1551
Size: 613 30 908
Fragments arranged by size:
908 613 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 124 127 130 139 142 145 154
Size: 49 75 3 3 9 3 3 9
Cuts at: 154 230 310 313 325 328 364 367 490
Size: 76 80 3 12 3 36 3 123
Cuts at: 490 887 1062 1551
Size: 397 175 489
Fragments arranged by size:
489 397 175 123 80 76 75 49
36 12 9 9 3 3 3 3
3 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 41 1551
Size: 41 1510
BsmBI CGTCTCn'nnnn_
Cuts at: 0 232 1551
Size: 232 1319
Bsp1286I G_dGCh'C
Cuts at: 0 41 55 104 259 424 482 1551
Size: 41 14 49 155 165 58 1069
Fragments arranged by size:
1069 165 155 58 49 41 14
BsrBI CCG'CTC
Cuts at: 0 413 1551
Size: 413 1138
BsrDI GCAATG_nn'
Cuts at: 0 737 1011 1551
Size: 737 274 540
Fragments arranged by size:
737 540 274
BsrFI r'CCGG_y
Cuts at: 0 605 1551
Size: 605 946
BstYI r'GATC_y
Cuts at: 0 828 1551
Size: 828 723
BtgI C'CryG_G
Cuts at: 0 490 1174 1551
Size: 490 684 377
Fragments arranged by size:
684 490 377
DraIII CAC_nnn'GTG
Cuts at: 0 967 1551
Size: 967 584
EaeI y'GGCC_r
Cuts at: 0 2 23 1177 1551
Size: 2 21 1154 374
Fragments arranged by size:
1154 374 21 2
EarI CTCTTCn'nnn_
Cuts at: 0 116 131 1489 1498 1551
Size: 116 15 1358 9 53
Fragments arranged by size:
1358 116 53 15 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 215 227 596 1551
Size: 160 55 12 369 955
Fragments arranged by size:
955 369 160 55 12
EcoICRI GAG'CTC
Cuts at: 0 39 1551
Size: 39 1512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 493 528 710 1041 1379 1551
Size: 52 441 35 182 331 338 172
Fragments arranged by size:
441 338 331 182 172 52 35
EcoO109I rG'GnC_Cy
Cuts at: 0 468 1551
Size: 468 1083
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 773 1551
Size: 773 778
HaeII r_GCGC'y
Cuts at: 0 169 269 365 431 1551
Size: 169 100 96 66 1120
Fragments arranged by size:
1120 169 100 96 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 613 645 1206 1238 1551
Size: 229 32 352 32 561 32 313
Fragments arranged by size:
561 352 313 229 32 32 32
KasI G'GCGC_C
Cuts at: 0 427 1551
Size: 427 1124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 611 1551
Size: 227 384 940
Fragments arranged by size:
940 384 227
MslI CAynn'nnrTG
Cuts at: 0 19 860 1551
Size: 19 841 691
Fragments arranged by size:
841 691 19
MspA1I CmG'CkG
Cuts at: 0 188 338 386 446 492 548 1551
Size: 188 150 48 60 46 56 1003
Fragments arranged by size:
1003 188 150 60 56 48 46
NaeI GCC'GGC
Cuts at: 0 607 1551
Size: 607 944
NarI GG'CG_CC
Cuts at: 0 428 1551
Size: 428 1123
NdeI CA'TA_TG
Cuts at: 0 953 1231 1551
Size: 953 278 320
Fragments arranged by size:
953 320 278
NgoMIV G'CCGG_C
Cuts at: 0 605 1551
Size: 605 946
NheI G'CTAG_C
Cuts at: 0 1032 1551
Size: 1032 519
NspI r_CATG'y
Cuts at: 0 859 1551
Size: 859 692
PciI A'CATG_T
Cuts at: 0 855 1551
Size: 855 696
SacI G_AGCT'C
Cuts at: 0 41 1551
Size: 41 1510
SacII CC_GC'GG
Cuts at: 0 493 1551
Size: 493 1058
ScaI AGT'ACT
Cuts at: 0 681 1296 1551
Size: 681 615 255
Fragments arranged by size:
681 615 255
SfoI GGC'GCC
Cuts at: 0 429 1551
Size: 429 1122
SmaI CCC'GGG
Cuts at: 0 74 1551
Size: 74 1477
SspI AAT'ATT
Cuts at: 0 774 1551
Size: 774 777
StyI C'CwwG_G
Cuts at: 0 482 1551
Size: 482 1069
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1371 1551
Size: 1371 180
TatI w'GTAC_w
Cuts at: 0 679 1294 1551
Size: 679 615 257
Fragments arranged by size:
679 615 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 832 864 1551
Size: 832 32 687
Fragments arranged by size:
832 687 32
Tth111I GACn'n_nGTC
Cuts at: 0 243 1551
Size: 243 1308
XmaI C'CCGG_G
Cuts at: 0 72 1551
Size: 72 1479
XmnI GAAnn'nnTTC
Cuts at: 0 1364 1551
Size: 1364 187
Enzymes that do cut:
AccI AcuI AflIII AvaI BanI BanII BbeI BbsI
BcgI BglI BglII BlpI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BsrBI BsrDI BsrFI BstYI BtgI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NspI PciI SacI SacII ScaI SfoI SmaI SspI
StyI TaqII TatI TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI Bme1580I BmgBI BpuEI BsaAI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NcoI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SalI SanDI SapI SbfI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XcmI XhoI ZraI