Restriction map for
pFN21AE2375
(Linear) (Six-base) MAPSORT of: KIEE2375.seq Check: 8797 from: 1 to: 2190
KIEE2375.orf
With 182 enzymes: SgfI *
October 20, 2011 13:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 679 1159 2190
Size: 341 338 480 1031
Fragments arranged by size:
1031 480 341 338
ApaI G_GGCC'C
Cuts at: 0 106 1055 1375 1558 1636 1780 1792 2190
Size: 106 949 320 183 78 144 12 398
Fragments arranged by size:
949 398 320 183 144 106 78 12
ApoI r'AATT_y
Cuts at: 0 352 2014 2190
Size: 352 1662 176
Fragments arranged by size:
1662 352 176
AscI GG'CGCG_CC
Cuts at: 0 68 2190
Size: 68 2122
AvaI C'yCGr_G
Cuts at: 0 1231 1726 1765 2190
Size: 1231 495 39 425
Fragments arranged by size:
1231 495 425 39
BanI G'GyrC_C
Cuts at: 0 1237 1459 1596 1675 1714 1753 1846 2190
Size: 1237 222 137 79 39 39 93 344
Fragments arranged by size:
1237 344 222 137 93 79 39 39
BanII G_rGCy'C
Cuts at: 0 99 106 308 1055 1294 1336 1354 1375
Size: 99 7 202 747 239 42 18 21
Cuts at: 1375 1537 1558 1636 1780 1792 2190
Size: 162 21 78 144 12 398
Fragments arranged by size:
747 398 239 202 162 144 99 78
42 21 21 18 12 7
BbeI G_GCGC'C
Cuts at: 0 1679 1718 2190
Size: 1679 39 472
Fragments arranged by size:
1679 472 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 423 606 2190
Size: 423 183 1584
Fragments arranged by size:
1584 423 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1455 1489 2190
Size: 1455 34 701
Fragments arranged by size:
1455 701 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 556 2190
Size: 556 1634
BfrBI ATG'CAT
Cuts at: 0 586 2190
Size: 586 1604
BglI GCCn_nnn'nGGC
Cuts at: 0 1379 1951 2190
Size: 1379 572 239
Fragments arranged by size:
1379 572 239
Bme1580I G_kGCm'C
Cuts at: 0 106 1055 1375 1558 1601 1636 1780 1792
Size: 106 949 320 183 43 35 144 12
Cuts at: 1792 2190
Size: 398
Fragments arranged by size:
949 398 320 183 144 106 43 35
12
BmgBI CAC'GTC
Cuts at: 0 825 2190
Size: 825 1365
BmrI ACTGGGnnnn_n'
Cuts at: 0 536 2190
Size: 536 1654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1383 1642 1684 1708 1723 1762 1943 2190
Size: 1383 259 42 24 15 39 181 247
Fragments arranged by size:
1383 259 247 181 42 39 24 15
Bpu10I CC'TnA_GC
Cuts at: 0 872 1579 2190
Size: 872 707 611
Fragments arranged by size:
872 707 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 2190
Size: 1501 689
BsaAI yAC'GTr
Cuts at: 0 300 2034 2190
Size: 300 1734 156
Fragments arranged by size:
1734 300 156
BsaBI GATnn'nnATC
Cuts at: 0 532 950 1089 2190
Size: 532 418 139 1101
Fragments arranged by size:
1101 532 418 139
BsaHI Gr'CG_yC
Cuts at: 0 1676 1715 2190
Size: 1676 39 475
Fragments arranged by size:
1676 475 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1175 1205 1381 1411 2190
Size: 1175 30 176 30 779
Fragments arranged by size:
1175 779 176 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 182 1160 1184 2190
Size: 182 978 24 1006
Fragments arranged by size:
1006 978 182 24
BseYI C'CCAG_C
Cuts at: 0 1377 2190
Size: 1377 813
BsiEI CG_ry'CG
Cuts at: 0 1506 2190
Size: 1506 684
BsiHKAI G_wGCw'C
Cuts at: 0 308 2190
Size: 308 1882
Bsp1286I G_dGCh'C
Cuts at: 0 99 106 308 1055 1294 1336 1354 1375
Size: 99 7 202 747 239 42 18 21
Cuts at: 1375 1537 1558 1601 1636 1780 1792 2190
Size: 162 21 43 35 144 12 398
Fragments arranged by size:
747 398 239 202 162 144 99 43
42 35 21 21 18 12 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 1594 1894 2190
Size: 135 1459 300 296
Fragments arranged by size:
1459 300 296 135
BsrBI CCG'CTC
Cuts at: 0 65 86 413 1495 1692 2190
Size: 65 21 327 1082 197 498
Fragments arranged by size:
1082 498 327 197 65 21
BsrDI GCAATG_nn'
Cuts at: 0 473 1154 2190
Size: 473 681 1036
Fragments arranged by size:
1036 681 473
BsrFI r'CCGG_y
Cuts at: 0 1240 1462 1657 2190
Size: 1240 222 195 533
Fragments arranged by size:
1240 533 222 195
BssHII G'CGCG_C
Cuts at: 0 68 2190
Size: 68 2122
BstBI TT'CG_AA
Cuts at: 0 2172 2190
Size: 2172 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 901 1662 1686 1872 1936 2190
Size: 901 761 24 186 64 254
Fragments arranged by size:
901 761 254 186 64 24
BstYI r'GATC_y
Cuts at: 0 438 1090 2190
Size: 438 652 1100
Fragments arranged by size:
1100 652 438
Bsu36I CC'TnA_GG
Cuts at: 0 107 2190
Size: 107 2083
BtgI C'CryG_G
Cuts at: 0 894 1272 1337 1424 1784 1829 2190
Size: 894 378 65 87 360 45 361
Fragments arranged by size:
894 378 361 360 87 65 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2061 2096 2190
Size: 2061 35 94
Fragments arranged by size:
2061 94 35
EaeI y'GGCC_r
Cuts at: 0 795 1490 1652 1826 1943 2028 2111 2154
Size: 795 695 162 174 117 85 83 43
Cuts at: 2154 2190
Size: 36
Fragments arranged by size:
795 695 174 162 117 85 83 43
36
EarI CTCTTCn'nnn_
Cuts at: 0 53 136 359 1135 2190
Size: 53 83 223 776 1055
Fragments arranged by size:
1055 776 223 83 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 2190
Size: 40 2150
EcoICRI GAG'CTC
Cuts at: 0 306 2190
Size: 306 1884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 679 1159 1383 1642 1684 1708 1723
Size: 341 338 480 224 259 42 24 15
Cuts at: 1723 1762 1943 2190
Size: 39 181 247
Fragments arranged by size:
480 341 338 259 247 224 181 42
39 24 15
EcoO109I rG'GnC_Cy
Cuts at: 0 103 842 988 1051 1052 1213 1555 1633
Size: 103 739 146 63 1 161 342 78
Cuts at: 1633 1777 2190
Size: 144 413
Fragments arranged by size:
739 413 342 161 146 144 103 78
63 1
EcoRI G'AATT_C
Cuts at: 0 352 2190
Size: 352 1838
HaeII r_GCGC'y
Cuts at: 0 1679 1718 1805 2190
Size: 1679 39 87 385
Fragments arranged by size:
1679 385 87 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1136 1168 1175 1197 1207 1229 2190
Size: 1136 32 7 22 10 22 961
Fragments arranged by size:
1136 961 32 22 22 10 7
HindIII A'AGCT_T
Cuts at: 0 1896 2190
Size: 1896 294
KasI G'GCGC_C
Cuts at: 0 1675 1714 2190
Size: 1675 39 476
Fragments arranged by size:
1675 476 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 2190
Size: 223 1967
MscI TGG'CCA
Cuts at: 0 797 1654 1828 1945 2190
Size: 797 857 174 117 245
Fragments arranged by size:
857 797 245 174 117
MslI CAynn'nnrTG
Cuts at: 0 973 1870 2053 2190
Size: 973 897 183 137
Fragments arranged by size:
973 897 183 137
MspA1I CmG'CkG
Cuts at: 0 801 1377 2190
Size: 801 576 813
Fragments arranged by size:
813 801 576
NaeI GCC'GGC
Cuts at: 0 1242 1464 2190
Size: 1242 222 726
Fragments arranged by size:
1242 726 222
NarI GG'CG_CC
Cuts at: 0 1676 1715 2190
Size: 1676 39 475
Fragments arranged by size:
1676 475 39
NcoI C'CATG_G
Cuts at: 0 894 1424 1784 1829 2190
Size: 894 530 360 45 361
Fragments arranged by size:
894 530 361 360 45
NdeI CA'TA_TG
Cuts at: 0 189 2190
Size: 189 2001
NgoMIV G'CCGG_C
Cuts at: 0 1240 1462 2190
Size: 1240 222 728
Fragments arranged by size:
1240 728 222
NsiI A_TGCA'T
Cuts at: 0 588 2190
Size: 588 1602
NspI r_CATG'y
Cuts at: 0 457 2190
Size: 457 1733
PasI CC'CwG_GG
Cuts at: 0 1195 1797 2190
Size: 1195 602 393
Fragments arranged by size:
1195 602 393
PflMI CCAn_nnn'nTGG
Cuts at: 0 1278 1425 2190
Size: 1278 147 765
Fragments arranged by size:
1278 765 147
PmlI CAC'GTG
Cuts at: 0 2034 2190
Size: 2034 156
PpuMI rG'GwC_Cy
Cuts at: 0 988 1213 2190
Size: 988 225 977
Fragments arranged by size:
988 977 225
PsiI TTA'TAA
Cuts at: 0 638 2190
Size: 638 1552
PspOMI G'GGCC_C
Cuts at: 0 102 1051 1371 1554 1632 1776 1788 2190
Size: 102 949 320 183 78 144 12 402
Fragments arranged by size:
949 402 320 183 144 102 78 12
PspXI vC'TCGA_Gb
Cuts at: 0 1726 1765 2190
Size: 1726 39 425
Fragments arranged by size:
1726 425 39
PvuII CAG'CTG
Cuts at: 0 801 2190
Size: 801 1389
SacI G_AGCT'C
Cuts at: 0 308 2190
Size: 308 1882
SexAI A'CCwGG_T
Cuts at: 0 266 510 2190
Size: 266 244 1680
Fragments arranged by size:
1680 266 244
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1379 1951 2190
Size: 1379 572 239
Fragments arranged by size:
1379 572 239
SfoI GGC'GCC
Cuts at: 0 1677 1716 2190
Size: 1677 39 474
Fragments arranged by size:
1677 474 39
SmlI C'TyrA_G
Cuts at: 0 1480 1726 1765 2190
Size: 1480 246 39 425
Fragments arranged by size:
1480 425 246 39
StuI AGG'CCT
Cuts at: 0 1954 2190
Size: 1954 236
StyI C'CwwG_G
Cuts at: 0 894 900 1424 1784 1829 2072 2190
Size: 894 6 524 360 45 243 118
Fragments arranged by size:
894 524 360 243 118 45 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 1447 1741 1822 2190
Size: 555 892 294 81 368
Fragments arranged by size:
892 555 368 294 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 530 562 1381 1413 2190
Size: 530 32 819 32 777
Fragments arranged by size:
819 777 530 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 901 1506 1527 1569 2190
Size: 901 605 21 42 621
Fragments arranged by size:
901 621 605 42 21
XhoI C'TCGA_G
Cuts at: 0 1726 1765 2190
Size: 1726 39 425
Fragments arranged by size:
1726 425 39
Enzymes that do cut:
AcuI ApaI ApoI AscI AvaI BanI BanII BbeI
BbsI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BstBI BstXI BstYI Bsu36I BtgI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PflMI PmlI
PpuMI PsiI PspOMI PspXI PvuII SacI SexAI SfiI
SfoI SmlI StuI StyI TaqII TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AseI
AsiSI AvrII BaeI BamHI BbvCI BclI BglII BlpI
BmtI BplI BsaI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssSI BstAPI BstEII BstZ17I BtgZI
BtsI ClaI DraI DraIII DrdI EagI EcoNI EcoRV
FalI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI NheI NotI NruI PacI PciI PfoI PmeI
PpiI PshAI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XmaI XmnI ZraI