Restriction map for
pFN21AE2372
(Linear) (Six-base) MAPSORT of: KIEE2372.seq Check: 9557 from: 1 to: 1971
KIEE2372.orf
With 182 enzymes: SgfI *
October 11, 2011 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 366 1971
Size: 27 339 1605
Fragments arranged by size:
1605 339 27
AccI GT'mk_AC
Cuts at: 0 233 1554 1971
Size: 233 1321 417
Fragments arranged by size:
1321 417 233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1162 1825 1922 1971
Size: 256 906 663 97 49
Fragments arranged by size:
906 663 256 97 49
AfeI AGC'GCT
Cuts at: 0 1922 1971
Size: 1922 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1658 1690 1971
Size: 1658 32 281
Fragments arranged by size:
1658 281 32
AlwNI CAG_nnn'CTG
Cuts at: 0 639 1971
Size: 639 1332
ApoI r'AATT_y
Cuts at: 0 211 256 302 451 906 1523 1971
Size: 211 45 46 149 455 617 448
Fragments arranged by size:
617 455 448 211 149 46 45
BamHI G'GATC_C
Cuts at: 0 156 1971
Size: 156 1815
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 1928 1971
Size: 217 1711 43
Fragments arranged by size:
1711 217 43
BfrBI ATG'CAT
Cuts at: 0 1708 1971
Size: 1708 263
BglI GCCn_nnn'nGGC
Cuts at: 0 667 1971
Size: 667 1304
BglII A'GATC_T
Cuts at: 0 264 1971
Size: 264 1707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1971
Size: 317 1654
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1971
Size: 269 1702
BsaAI yAC'GTr
Cuts at: 0 1415 1971
Size: 1415 556
BsaHI Gr'CG_yC
Cuts at: 0 598 1971
Size: 598 1373
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 1971
Size: 57 30 1884
Fragments arranged by size:
1884 57 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 78 874 1584 1971
Size: 73 5 796 710 387
Fragments arranged by size:
796 710 387 73 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1971
Size: 1475 496
BsmI GAATG_Cn'
Cuts at: 0 627 931 1606 1823 1971
Size: 627 304 675 217 148
Fragments arranged by size:
675 627 304 217 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 366 1971
Size: 27 339 1605
Fragments arranged by size:
1605 339 27
BsrDI GCAATG_nn'
Cuts at: 0 283 1971
Size: 283 1688
BssSI C'ACGA_G
Cuts at: 0 931 1971
Size: 931 1040
BstBI TT'CG_AA
Cuts at: 0 1308 1971
Size: 1308 663
BstYI r'GATC_y
Cuts at: 0 156 264 1176 1971
Size: 156 108 912 795
Fragments arranged by size:
912 795 156 108
DraI TTT'AAA
Cuts at: 0 209 368 1971
Size: 209 159 1603
Fragments arranged by size:
1603 209 159
EarI CTCTTCn'nnn_
Cuts at: 0 851 894 1310 1971
Size: 851 43 416 661
Fragments arranged by size:
851 661 416 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1475 1971
Size: 1475 496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 317 1162 1825 1922 1971
Size: 256 61 845 663 97 49
Fragments arranged by size:
845 663 256 97 61 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1661 1971
Size: 1661 310
EcoO109I rG'GnC_Cy
Cuts at: 0 1471 1971
Size: 1471 500
EcoRI G'AATT_C
Cuts at: 0 906 1971
Size: 906 1065
EcoRV GAT'ATC
Cuts at: 0 1634 1971
Size: 1634 337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 859 1971
Size: 859 1112
HaeII r_GCGC'y
Cuts at: 0 1924 1971
Size: 1924 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1569 1601 1711 1743 1971
Size: 1569 32 110 32 228
Fragments arranged by size:
1569 228 110 32 32
HindIII A'AGCT_T
Cuts at: 0 1102 1971
Size: 1102 869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1275 1769 1971
Size: 1275 494 202
Fragments arranged by size:
1275 494 202
MspA1I CmG'CkG
Cuts at: 0 870 1971
Size: 870 1101
NsiI A_TGCA'T
Cuts at: 0 1710 1971
Size: 1710 261
NspI r_CATG'y
Cuts at: 0 295 1971
Size: 295 1676
PasI CC'CwG_GG
Cuts at: 0 1951 1971
Size: 1951 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 1573 1971
Size: 1573 398
PfoI T'CCnGG_A
Cuts at: 0 1043 1971
Size: 1043 928
PmlI CAC'GTG
Cuts at: 0 1415 1971
Size: 1415 556
PpuMI rG'GwC_Cy
Cuts at: 0 1471 1971
Size: 1471 500
PstI C_TGCA'G
Cuts at: 0 1134 1971
Size: 1134 837
SfcI C'TryA_G
Cuts at: 0 319 865 1130 1355 1971
Size: 319 546 265 225 616
Fragments arranged by size:
616 546 319 265 225
SmlI C'TyrA_G
Cuts at: 0 284 1971
Size: 284 1687
SpeI A'CTAG_T
Cuts at: 0 24 1971
Size: 24 1947
StyI C'CwwG_G
Cuts at: 0 307 1971
Size: 307 1664
TatI w'GTAC_w
Cuts at: 0 280 1089 1971
Size: 280 809 882
Fragments arranged by size:
882 809 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 422 454 1971
Size: 422 32 1517
Fragments arranged by size:
1517 422 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1876 1971
Size: 1876 95
XmnI GAAnn'nnTTC
Cuts at: 0 197 1618 1971
Size: 197 1421 353
Fragments arranged by size:
1421 353 197
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApoI BamHI
BbsI BfrBI BglI BglII BpmI BpuEI BsaAI BsaHI
BsaXI BseRI BsgI BsmI BspMI BsrDI BssSI BstBI
BstYI DraI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HindIII MmeI MspA1I NsiI
NspI PasI PflMI PfoI PmlI PpuMI PstI SfcI
SmlI SpeI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BanII BbeI BbvCI BcgI BciVI BclI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EcoICRI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI