Restriction map for
pFN21AE2371
(Linear) (Six-base) MAPSORT of: KIEE2371.seq Check: 9058 from: 1 to: 2370
KIEE2371.orf
With 182 enzymes: SgfI *
October 11, 2011 13:28 ..
AatII G_ACGT'C
Cuts at: 0 722 2370
Size: 722 1648
AccI GT'mk_AC
Cuts at: 0 1223 2370
Size: 1223 1147
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 602 1090 1783 1857 2370
Size: 376 226 488 693 74 513
Fragments arranged by size:
693 513 488 376 226 74
AflIII A'CryG_T
Cuts at: 0 1316 2370
Size: 1316 1054
AleI CACnn'nnGTG
Cuts at: 0 1180 2370
Size: 1180 1190
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1546 1578 2370
Size: 1546 32 792
Fragments arranged by size:
1546 792 32
AlwNI CAG_nnn'CTG
Cuts at: 0 840 2370
Size: 840 1530
ApaLI G'TGCA_C
Cuts at: 0 119 311 2370
Size: 119 192 2059
Fragments arranged by size:
2059 192 119
ApoI r'AATT_y
Cuts at: 0 2350 2370
Size: 2350 20
AvaI C'yCGr_G
Cuts at: 0 823 1388 2256 2370
Size: 823 565 868 114
Fragments arranged by size:
868 823 565 114
AvrII C'CTAG_G
Cuts at: 0 1452 2370
Size: 1452 918
BamHI G'GATC_C
Cuts at: 0 563 2370
Size: 563 1807
BanI G'GyrC_C
Cuts at: 0 24 443 712 2075 2370
Size: 24 419 269 1363 295
Fragments arranged by size:
1363 419 295 269 24
BbeI G_GCGC'C
Cuts at: 0 447 716 2079 2370
Size: 447 269 1363 291
Fragments arranged by size:
1363 447 291 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 511 1602 2233 2258 2370
Size: 180 331 1091 631 25 112
Fragments arranged by size:
1091 631 331 180 112 25
BbvCI CC'TCA_GC
Cuts at: 0 2131 2370
Size: 2131 239
BciVI GTATCCnnnnn_n'
Cuts at: 0 1958 2370
Size: 1958 412
BfrBI ATG'CAT
Cuts at: 0 2332 2370
Size: 2332 38
BglI GCCn_nnn'nGGC
Cuts at: 0 739 2370
Size: 739 1631
Bme1580I G_kGCm'C
Cuts at: 0 123 315 2370
Size: 123 192 2055
Fragments arranged by size:
2055 192 123
BmgBI CAC'GTC
Cuts at: 0 2235 2370
Size: 2235 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 252 302 999 1146 2370
Size: 50 202 50 697 147 1224
Fragments arranged by size:
1224 697 202 147 50 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2067 2370
Size: 2067 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1018 1285 2370
Size: 154 864 267 1085
Fragments arranged by size:
1085 864 267 154
Bpu10I CC'TnA_GC
Cuts at: 0 2131 2370
Size: 2131 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 277 994 1518 2370
Size: 277 717 524 852
Fragments arranged by size:
852 717 524 277
BsaHI Gr'CG_yC
Cuts at: 0 444 713 719 790 2076 2370
Size: 444 269 6 71 1286 294
Fragments arranged by size:
1286 444 294 269 71 6
BsaWI w'CCGG_w
Cuts at: 0 1109 2144 2370
Size: 1109 1035 226
Fragments arranged by size:
1109 1035 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 165 195 235 265 1843 1873 2370
Size: 165 30 40 30 1578 30 497
Fragments arranged by size:
1578 497 165 40 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 254 351 412 643 1408 1411 1993
Size: 170 84 97 61 231 765 3 582
Cuts at: 1993 1996 2148 2370
Size: 3 152 222
Fragments arranged by size:
765 582 231 222 170 152 97 84
61 3 3
BseYI C'CCAG_C
Cuts at: 0 1673 2370
Size: 1673 697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1258 2370
Size: 853 405 1112
Fragments arranged by size:
1112 853 405
BsiHKAI G_wGCw'C
Cuts at: 0 123 315 851 1472 2370
Size: 123 192 536 621 898
Fragments arranged by size:
898 621 536 192 123
BsiWI C'GTAC_G
Cuts at: 0 1142 2370
Size: 1142 1228
BsmBI CGTCTCn'nnnn_
Cuts at: 0 781 1377 2055 2241 2370
Size: 781 596 678 186 129
Fragments arranged by size:
781 678 596 186 129
Bsp1286I G_dGCh'C
Cuts at: 0 123 315 851 1472 2370
Size: 123 192 536 621 898
Fragments arranged by size:
898 621 536 192 123
BspEI T'CCGG_A
Cuts at: 0 1109 2144 2370
Size: 1109 1035 226
Fragments arranged by size:
1109 1035 226
BspHI T'CATG_A
Cuts at: 0 1730 2370
Size: 1730 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 593 2370
Size: 517 76 1777
Fragments arranged by size:
1777 517 76
BsrBI CCG'CTC
Cuts at: 0 672 2370
Size: 672 1698
BsrDI GCAATG_nn'
Cuts at: 0 1505 2018 2370
Size: 1505 513 352
Fragments arranged by size:
1505 513 352
BsrFI r'CCGG_y
Cuts at: 0 90 709 1244 2370
Size: 90 619 535 1126
Fragments arranged by size:
1126 619 535 90
BsrGI T'GTAC_A
Cuts at: 0 1721 2370
Size: 1721 649
BssSI C'ACGA_G
Cuts at: 0 850 881 1651 2370
Size: 850 31 770 719
Fragments arranged by size:
850 770 719 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1254 1281 2370
Size: 1254 27 1089
Fragments arranged by size:
1254 1089 27
BstEII G'GTnAC_C
Cuts at: 0 909 1918 2370
Size: 909 1009 452
Fragments arranged by size:
1009 909 452
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 201 1668 1914 2370
Size: 21 180 1467 246 456
Fragments arranged by size:
1467 456 246 180 21
BstYI r'GATC_y
Cuts at: 0 563 1064 1533 2370
Size: 563 501 469 837
Fragments arranged by size:
837 563 501 469
Bsu36I CC'TnA_GG
Cuts at: 0 266 2370
Size: 266 2104
BtgI C'CryG_G
Cuts at: 0 200 914 1193 1661 1712 2370
Size: 200 714 279 468 51 658
Fragments arranged by size:
714 658 468 279 200 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 645 672 687 2370
Size: 645 27 15 1683
Fragments arranged by size:
1683 645 27 15
BtsI GCAGTG_nn'
Cuts at: 0 226 1889 2370
Size: 226 1663 481
Fragments arranged by size:
1663 481 226
EaeI y'GGCC_r
Cuts at: 0 320 917 1040 1658 2168 2370
Size: 320 597 123 618 510 202
Fragments arranged by size:
618 597 510 320 202 123
EarI CTCTTCn'nnn_
Cuts at: 0 79 109 323 578 1900 2370
Size: 79 30 214 255 1322 470
Fragments arranged by size:
1322 470 255 214 79 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 1075 2370
Size: 109 966 1295
Fragments arranged by size:
1295 966 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 376 602 1018 1090 1285 1783 1857
Size: 154 222 226 416 72 195 498 74
Cuts at: 1857 2370
Size: 513
Fragments arranged by size:
513 498 416 226 222 195 154 74
72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 517 2370
Size: 517 1853
EcoO109I rG'GnC_Cy
Cuts at: 0 740 2260 2370
Size: 740 1520 110
Fragments arranged by size:
1520 740 110
EcoRI G'AATT_C
Cuts at: 0 2350 2370
Size: 2350 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 759 1609 2370
Size: 384 375 850 761
Fragments arranged by size:
850 761 384 375
HaeII r_GCGC'y
Cuts at: 0 447 655 716 2079 2370
Size: 447 208 61 1363 291
Fragments arranged by size:
1363 447 291 208 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 165 197 235 267 274 306
Size: 12 32 121 32 38 32 7 32
Cuts at: 306 1525 1557 1843 1875 2370
Size: 1219 32 286 32 495
Fragments arranged by size:
1219 495 286 121 38 32 32 32
32 32 32 12 7
HincII GTy'rAC
Cuts at: 0 723 2370
Size: 723 1647
KasI G'GCGC_C
Cuts at: 0 443 712 2075 2370
Size: 443 269 1363 295
Fragments arranged by size:
1363 443 295 269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 518 2208 2370
Size: 233 285 1690 162
Fragments arranged by size:
1690 285 233 162
MscI TGG'CCA
Cuts at: 0 322 1660 2370
Size: 322 1338 710
Fragments arranged by size:
1338 710 322
MslI CAynn'nnrTG
Cuts at: 0 1180 1492 1717 2370
Size: 1180 312 225 653
Fragments arranged by size:
1180 653 312 225
MspA1I CmG'CkG
Cuts at: 0 318 837 1032 1038 1272 2370
Size: 318 519 195 6 234 1098
Fragments arranged by size:
1098 519 318 234 195 6
NaeI GCC'GGC
Cuts at: 0 92 711 2370
Size: 92 619 1659
Fragments arranged by size:
1659 619 92
NarI GG'CG_CC
Cuts at: 0 444 713 2076 2370
Size: 444 269 1363 294
Fragments arranged by size:
1363 444 294 269
NcoI C'CATG_G
Cuts at: 0 200 1661 1712 2370
Size: 200 1461 51 658
Fragments arranged by size:
1461 658 200 51
NdeI CA'TA_TG
Cuts at: 0 1289 2370
Size: 1289 1081
NgoMIV G'CCGG_C
Cuts at: 0 90 709 2370
Size: 90 619 1661
Fragments arranged by size:
1661 619 90
NsiI A_TGCA'T
Cuts at: 0 2334 2370
Size: 2334 36
NspI r_CATG'y
Cuts at: 0 812 960 1320 1686 1722 2016 2370
Size: 812 148 360 366 36 294 354
Fragments arranged by size:
812 366 360 354 294 148 36
PasI CC'CwG_GG
Cuts at: 0 1668 2370
Size: 1668 702
PciI A'CATG_T
Cuts at: 0 1316 2370
Size: 1316 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 1713 2370
Size: 1713 657
PfoI T'CCnGG_A
Cuts at: 0 131 1120 2302 2354 2370
Size: 131 989 1182 52 16
Fragments arranged by size:
1182 989 131 52 16
PshAI GACnn'nnGTC
Cuts at: 0 1152 1369 2233 2370
Size: 1152 217 864 137
Fragments arranged by size:
1152 864 217 137
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2370
Size: 1672 32 666
Fragments arranged by size:
1672 666 32
PstI C_TGCA'G
Cuts at: 0 428 1037 1448 1541 1858 2084 2370
Size: 428 609 411 93 317 226 286
Fragments arranged by size:
609 428 411 317 286 226 93
PvuII CAG'CTG
Cuts at: 0 318 837 1038 2370
Size: 318 519 201 1332
Fragments arranged by size:
1332 519 318 201
SapI GCTCTTCn'nnn_
Cuts at: 0 323 2370
Size: 323 2047
SfcI C'TryA_G
Cuts at: 0 424 1033 1444 1537 1854 2080 2370
Size: 424 609 411 93 317 226 290
Fragments arranged by size:
609 424 411 317 290 226 93
SfoI GGC'GCC
Cuts at: 0 445 714 2077 2370
Size: 445 269 1363 293
Fragments arranged by size:
1363 445 293 269
SgrAI Cr'CCGG_yG
Cuts at: 0 709 2370
Size: 709 1661
SmaI CCC'GGG
Cuts at: 0 1390 2258 2370
Size: 1390 868 112
Fragments arranged by size:
1390 868 112
SphI G_CATG'C
Cuts at: 0 812 2370
Size: 812 1558
StuI AGG'CCT
Cuts at: 0 523 634 1913 2370
Size: 523 111 1279 457
Fragments arranged by size:
1279 523 457 111
StyI C'CwwG_G
Cuts at: 0 14 200 323 548 734 1415 1452 1661
Size: 14 186 123 225 186 681 37 209
Cuts at: 1661 1712 1811 2054 2370
Size: 51 99 243 316
Fragments arranged by size:
681 316 243 225 209 186 186 123
99 51 37 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1906 2370
Size: 1906 464
TatI w'GTAC_w
Cuts at: 0 620 1073 1616 1721 2370
Size: 620 453 543 105 649
Fragments arranged by size:
649 620 543 453 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 2370
Size: 113 32 2225
Fragments arranged by size:
2225 113 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 2097 2370
Size: 315 1782 273
Fragments arranged by size:
1782 315 273
XmaI C'CCGG_G
Cuts at: 0 1388 2256 2370
Size: 1388 868 114
Fragments arranged by size:
1388 868 114
ZraI GAC'GTC
Cuts at: 0 720 2370
Size: 720 1650
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI AlwNI ApaLI
ApoI AvaI AvrII BamHI BanI BbeI BbsI BbvCI
BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PshAI
PsrI PstI PvuII SapI SfcI SfoI SgrAI SmaI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI AscI AseI AsiSI BaeI BanII BcgI BclI
BglII BlpI BmtI BpuEI BsaAI BsaBI BsiEI BsmI
BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI NheI NotI NruI PacI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI