Restriction map for
pFN21AE2367
(Linear) (Six-base) MAPSORT of: KIEE2367.seq Check: 8423 from: 1 to: 6435
KIEE2367.orf
With 182 enzymes: SgfI *
August 4, 2011 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1191 6435
Size: 1191 5244
AatII G_ACGT'C
Cuts at: 0 71 3305 6435
Size: 71 3234 3130
Fragments arranged by size:
3234 3130 71
AccI GT'mk_AC
Cuts at: 0 71 6435
Size: 71 6364
AclI AA'CG_TT
Cuts at: 0 4735 6435
Size: 4735 1700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 262 533 2251 2832 3178 3928 4627
Size: 61 201 271 1718 581 346 750 699
Cuts at: 4627 4640 4796 5464 6435
Size: 13 156 668 971
Fragments arranged by size:
1718 971 750 699 668 581 346 271
201 156 61 13
AflII C'TTAA_G
Cuts at: 0 4423 6435
Size: 4423 2012
AflIII A'CryG_T
Cuts at: 0 716 1382 1850 3185 4891 6435
Size: 716 666 468 1335 1706 1544
Fragments arranged by size:
1706 1544 1335 716 666 468
AhdI GACnn_n'nnGTC
Cuts at: 0 1367 2638 3749 6269 6435
Size: 1367 1271 1111 2520 166
Fragments arranged by size:
2520 1367 1271 1111 166
AleI CACnn'nnGTG
Cuts at: 0 3282 6435
Size: 3282 3153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2341 2373 6435
Size: 2341 32 4062
Fragments arranged by size:
4062 2341 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1888 4663 5181 6018 6036 6435
Size: 1888 2775 518 837 18 399
Fragments arranged by size:
2775 1888 837 518 399 18
ApaI G_GGCC'C
Cuts at: 0 993 4632 6435
Size: 993 3639 1803
Fragments arranged by size:
3639 1803 993
ApaLI G'TGCA_C
Cuts at: 0 1061 6435
Size: 1061 5374
ApoI r'AATT_y
Cuts at: 0 549 1783 2258 2833 2904 4569 4800 6435
Size: 549 1234 475 575 71 1665 231 1635
Fragments arranged by size:
1665 1635 1234 575 549 475 231 71
AseI AT'TA_AT
Cuts at: 0 2077 6435
Size: 2077 4358
AvaI C'yCGr_G
Cuts at: 0 55 978 1868 6435
Size: 55 923 890 4567
Fragments arranged by size:
4567 923 890 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 966 999 1839 1872 6435
Size: 966 33 840 33 4563
Fragments arranged by size:
4563 966 840 33 33
BamHI G'GATC_C
Cuts at: 0 761 1687 2922 6138 6435
Size: 761 926 1235 3216 297
Fragments arranged by size:
3216 1235 926 761 297
BanI G'GyrC_C
Cuts at: 0 5587 6435
Size: 5587 848
BanII G_rGCy'C
Cuts at: 0 993 1027 3414 4632 5216 6064 6435
Size: 993 34 2387 1218 584 848 371
Fragments arranged by size:
2387 1218 993 848 584 371 34
BbeI G_GCGC'C
Cuts at: 0 5591 6435
Size: 5591 844
BbsI GAAGACnn'nnnn_
Cuts at: 0 1824 6435
Size: 1824 4611
BbvCI CC'TCA_GC
Cuts at: 0 2958 5768 6435
Size: 2958 2810 667
Fragments arranged by size:
2958 2810 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5606 5640 6435
Size: 5606 34 795
Fragments arranged by size:
5606 795 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5792 6435
Size: 5792 643
BclI T'GATC_A
Cuts at: 0 476 2388 2694 3998 5099 6435
Size: 476 1912 306 1304 1101 1336
Fragments arranged by size:
1912 1336 1304 1101 476 306
BfrBI ATG'CAT
Cuts at: 0 2780 4759 6435
Size: 2780 1979 1676
Fragments arranged by size:
2780 1979 1676
BglI GCCn_nnn'nGGC
Cuts at: 0 1010 5917 6435
Size: 1010 4907 518
Fragments arranged by size:
4907 1010 518
BlpI GC'TnA_GC
Cuts at: 0 171 916 5255 6435
Size: 171 745 4339 1180
Fragments arranged by size:
4339 1180 745 171
Bme1580I G_kGCm'C
Cuts at: 0 897 993 1065 4632 6128 6435
Size: 897 96 72 3567 1496 307
Fragments arranged by size:
3567 1496 897 307 96 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 4605 4635 6435
Size: 4605 30 1800
Fragments arranged by size:
4605 1800 30
BmtI G_CTAG'C
Cuts at: 0 709 6435
Size: 709 5726
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2973 6435
Size: 2973 3462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 612 1615 2006 2353 2368 2589 3316
Size: 176 436 1003 391 347 15 221 727
Cuts at: 3316 3973 4330 4450 4518 4972 5158 5207 5483
Size: 657 357 120 68 454 186 49 276
Cuts at: 5483 5493 5889 6172 6352 6435
Size: 10 396 283 180 83
Fragments arranged by size:
1003 727 657 454 436 396 391 357
347 283 276 221 186 180 176 120
83 68 49 15 10
Bpu10I CC'TnA_GC
Cuts at: 0 2958 5583 5768 6435
Size: 2958 2625 185 667
Fragments arranged by size:
2958 2625 667 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3419 3973 5319 6435
Size: 3419 554 1346 1116
Fragments arranged by size:
3419 1346 1116 554
BsaI GGTCTCn'nnnn_
Cuts at: 0 27 2834 4990 4998 6141 6277 6435
Size: 27 2807 2156 8 1143 136 158
Fragments arranged by size:
2807 2156 1143 158 136 27 8
BsaAI yAC'GTr
Cuts at: 0 3186 6435
Size: 3186 3249
BsaBI GATnn'nnATC
Cuts at: 0 800 2393 5348 6435
Size: 800 1593 2955 1087
Fragments arranged by size:
2955 1593 1087 800
BsaHI Gr'CG_yC
Cuts at: 0 68 900 3302 3359 5588 6435
Size: 68 832 2402 57 2229 847
Fragments arranged by size:
2402 2229 847 832 68 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 2341 2357 2371 2387 4970 5000
Size: 610 30 1701 16 14 16 2583 30
Cuts at: 5000 6103 6133 6435
Size: 1103 30 302
Fragments arranged by size:
2583 1701 1103 610 302 30 30 30
16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1886 3093 4617 5125 5212 5388 6175 6435
Size: 1886 1207 1524 508 87 176 787 260
Fragments arranged by size:
1886 1524 1207 787 508 260 176 87
BseYI C'CCAG_C
Cuts at: 0 994 2985 3147 4297 4713 5046 5459 5893
Size: 994 1991 162 1150 416 333 413 434
Cuts at: 5893 6203 6212 6218 6230 6236 6290 6329 6435
Size: 310 9 6 12 6 54 39 106
Fragments arranged by size:
1991 1150 994 434 416 413 333 310
162 106 54 39 12 9 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1328 3108 3328 4618 6086 6435
Size: 1051 277 1780 220 1290 1468 349
Fragments arranged by size:
1780 1468 1290 1051 349 277 220
BsiEI CG_ry'CG
Cuts at: 0 1903 5921 6350 6435
Size: 1903 4018 429 85
Fragments arranged by size:
4018 1903 429 85
BsiHKAI G_wGCw'C
Cuts at: 0 1065 2278 3414 4609 6435
Size: 1065 1213 1136 1195 1826
Fragments arranged by size:
1826 1213 1195 1136 1065
BsmI GAATG_Cn'
Cuts at: 0 2007 3645 6435
Size: 2007 1638 2790
Fragments arranged by size:
2790 2007 1638
Bsp1286I G_dGCh'C
Cuts at: 0 897 993 1027 1065 2278 3414 4609 4632
Size: 897 96 34 38 1213 1136 1195 23
Cuts at: 4632 5216 6064 6128 6435
Size: 584 848 64 307
Fragments arranged by size:
1213 1195 1136 897 848 584 307 96
64 38 34 23
BspHI T'CATG_A
Cuts at: 0 2160 2933 3044 6435
Size: 2160 773 111 3391
Fragments arranged by size:
3391 2160 773 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1191 1245 4426 6017 6435
Size: 1191 54 3181 1591 418
Fragments arranged by size:
3181 1591 1191 418 54
BsrDI GCAATG_nn'
Cuts at: 0 691 3260 3774 4552 6287 6435
Size: 691 2569 514 778 1735 148
Fragments arranged by size:
2569 1735 778 691 514 148
BsrGI T'GTAC_A
Cuts at: 0 3802 6435
Size: 3802 2633
BssSI C'ACGA_G
Cuts at: 0 4179 5446 6435
Size: 4179 1267 989
Fragments arranged by size:
4179 1267 989
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4542 6318 6435
Size: 4542 1776 117
Fragments arranged by size:
4542 1776 117
BstBI TT'CG_AA
Cuts at: 0 2908 6435
Size: 2908 3527
BstEII G'GTnAC_C
Cuts at: 0 1907 6435
Size: 1907 4528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1581 6435
Size: 1581 4854
BstYI r'GATC_y
Cuts at: 0 761 1191 1687 2922 2993 4771 6138 6435
Size: 761 430 496 1235 71 1778 1367 297
Fragments arranged by size:
1778 1367 1235 761 496 430 297 71
Bsu36I CC'TnA_GG
Cuts at: 0 5195 6134 6435
Size: 5195 939 301
Fragments arranged by size:
5195 939 301
BtgI C'CryG_G
Cuts at: 0 1436 1574 1903 5017 6435
Size: 1436 138 329 3114 1418
Fragments arranged by size:
3114 1436 1418 329 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 653 2931 3642 4236 5609 5843 6435
Size: 653 2278 711 594 1373 234 592
Fragments arranged by size:
2278 1373 711 653 594 592 234
BtsI GCAGTG_nn'
Cuts at: 0 1316 1886 3964 5179 5883 6435
Size: 1316 570 2078 1215 704 552
Fragments arranged by size:
2078 1316 1215 704 570 552
DraI TTT'AAA
Cuts at: 0 3596 6435
Size: 3596 2839
DraIII CAC_nnn'GTG
Cuts at: 0 4613 5432 6435
Size: 4613 819 1003
Fragments arranged by size:
4613 1003 819
EaeI y'GGCC_r
Cuts at: 0 2 596 1900 3011 4037 5918 6347 6435
Size: 2 594 1304 1111 1026 1881 429 88
Fragments arranged by size:
1881 1304 1111 1026 594 429 88 2
EagI C'GGCC_G
Cuts at: 0 1900 5918 6347 6435
Size: 1900 4018 429 88
Fragments arranged by size:
4018 1900 429 88
EarI CTCTTCn'nnn_
Cuts at: 0 1594 1810 2050 2266 2851 3335 3409 4144
Size: 1594 216 240 216 585 484 74 735
Cuts at: 4144 4275 4298 4772 5088 5198 5204 5615 6435
Size: 131 23 474 316 110 6 411 820
Fragments arranged by size:
1594 820 735 585 484 474 411 316
240 216 216 131 110 74 23 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1810 4552 6435
Size: 1810 2742 1883
Fragments arranged by size:
2742 1883 1810
EcoICRI GAG'CTC
Cuts at: 0 3412 6435
Size: 3412 3023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 176 262 533 612 1615 2006 2251
Size: 61 115 86 271 79 1003 391 245
Cuts at: 2251 2353 2368 2589 2832 3178 3316 3928 3973
Size: 102 15 221 243 346 138 612 45
Cuts at: 3973 4330 4450 4518 4627 4640 4796 4972 5158
Size: 357 120 68 109 13 156 176 186
Cuts at: 5158 5207 5464 5483 5493 5889 6172 6352 6435
Size: 49 257 19 10 396 283 180 83
Fragments arranged by size:
1003 612 396 391 357 346 283 271
257 245 243 221 186 180 176 156
138 120 115 109 102 86 83 79
68 61 49 45 19 15 13 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6133 6435
Size: 6133 302
EcoO109I rG'GnC_Cy
Cuts at: 0 81 989 990 1740 4017 4629 6435
Size: 81 908 1 750 2277 612 1806
Fragments arranged by size:
2277 1806 908 750 612 81 1
EcoRV GAT'ATC
Cuts at: 0 569 580 2250 3651 6435
Size: 569 11 1670 1401 2784
Fragments arranged by size:
2784 1670 1401 569 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 3488 6435
Size: 147 3341 2947
Fragments arranged by size:
3341 2947 147
FspI TGC'GCA
Cuts at: 0 5750 6435
Size: 5750 685
HaeII r_GCGC'y
Cuts at: 0 48 2667 5583 5591 6435
Size: 48 2619 2916 8 844
Fragments arranged by size:
2916 2619 844 48 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1130 1353 1385 1434 1466 1645 1677
Size: 1098 32 223 32 49 32 179 32
Cuts at: 1677 1819 1851 1959 1991 2624 2656 3735 3767
Size: 142 32 108 32 633 32 1079 32
Cuts at: 3767 4009 4041 4825 4857 5203 5235 5400 5432
Size: 242 32 784 32 346 32 165 32
Cuts at: 5432 6012 6044 6255 6287 6435
Size: 580 32 211 32 148
Fragments arranged by size:
1098 1079 784 633 580 346 242 223
211 179 165 148 142 108 49 32
32 32 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3754 4390 4479 4668 6435
Size: 3754 636 89 189 1767
Fragments arranged by size:
3754 1767 636 189 89
HindIII A'AGCT_T
Cuts at: 0 244 2442 5650 6435
Size: 244 2198 3208 785
Fragments arranged by size:
3208 2198 785 244
HpaI GTT'AAC
Cuts at: 0 4479 6435
Size: 4479 1956
KasI G'GCGC_C
Cuts at: 0 5587 6435
Size: 5587 848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1733 2365 5733 5954 6435
Size: 1367 366 632 3368 221 481
Fragments arranged by size:
3368 1367 632 481 366 221
MscI TGG'CCA
Cuts at: 0 598 6435
Size: 598 5837
MslI CAynn'nnrTG
Cuts at: 0 1579 1851 3068 3282 3514 6435
Size: 1579 272 1217 214 232 2921
Fragments arranged by size:
2921 1579 1217 272 232 214
MspA1I CmG'CkG
Cuts at: 0 39 1035 1905 2229 3496 4297 5254 5577
Size: 39 996 870 324 1267 801 957 323
Cuts at: 5577 6435
Size: 858
Fragments arranged by size:
1267 996 957 870 858 801 324 323
39
NarI GG'CG_CC
Cuts at: 0 5588 6435
Size: 5588 847
NcoI C'CATG_G
Cuts at: 0 1436 1574 6435
Size: 1436 138 4861
Fragments arranged by size:
4861 1436 138
NheI G'CTAG_C
Cuts at: 0 705 6435
Size: 705 5730
NsiI A_TGCA'T
Cuts at: 0 2782 4761 6435
Size: 2782 1979 1674
Fragments arranged by size:
2782 1979 1674
NspI r_CATG'y
Cuts at: 0 720 1854 2743 3316 3386 4674 4713 4895
Size: 720 1134 889 573 70 1288 39 182
Cuts at: 4895 5571 6435
Size: 676 864
Fragments arranged by size:
1288 1134 889 864 720 676 573 182
70 39
PasI CC'CwG_GG
Cuts at: 0 4765 6435
Size: 4765 1670
PciI A'CATG_T
Cuts at: 0 716 1850 4891 6435
Size: 716 1134 3041 1544
Fragments arranged by size:
3041 1544 1134 716
PflMI CCAn_nnn'nTGG
Cuts at: 0 5432 6435
Size: 5432 1003
PfoI T'CCnGG_A
Cuts at: 0 257 722 764 1592 6435
Size: 257 465 42 828 4843
Fragments arranged by size:
4843 828 465 257 42
PmlI CAC'GTG
Cuts at: 0 3186 6435
Size: 3186 3249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2341 2373 6435
Size: 2341 32 4062
Fragments arranged by size:
4062 2341 32
PpuMI rG'GwC_Cy
Cuts at: 0 81 4017 6435
Size: 81 3936 2418
Fragments arranged by size:
3936 2418 81
PspOMI G'GGCC_C
Cuts at: 0 989 4628 6435
Size: 989 3639 1807
Fragments arranged by size:
3639 1807 989
PspXI vC'TCGA_Gb
Cuts at: 0 978 6435
Size: 978 5457
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2844 2876 6435
Size: 2844 32 3559
Fragments arranged by size:
3559 2844 32
PstI C_TGCA'G
Cuts at: 0 836 1202 3866 4104 5526 5660 5795 6014
Size: 836 366 2664 238 1422 134 135 219
Cuts at: 6014 6435
Size: 421
Fragments arranged by size:
2664 1422 836 421 366 238 219 135
134
PvuII CAG'CTG
Cuts at: 0 1035 2229 6435
Size: 1035 1194 4206
Fragments arranged by size:
4206 1194 1035
SacI G_AGCT'C
Cuts at: 0 3414 6435
Size: 3414 3021
SacII CC_GC'GG
Cuts at: 0 1906 6435
Size: 1906 4529
SapI GCTCTTCn'nnn_
Cuts at: 0 1810 2266 4298 5204 6435
Size: 1810 456 2032 906 1231
Fragments arranged by size:
2032 1810 1231 906 456
SbfI CC_TGCA'GG
Cuts at: 0 5526 6435
Size: 5526 909
SexAI A'CCwGG_T
Cuts at: 0 1793 6435
Size: 1793 4642
SfcI C'TryA_G
Cuts at: 0 832 1198 3021 3215 3862 4100 4619 4967
Size: 832 366 1823 194 647 238 519 348
Cuts at: 4967 5522 5572 5656 5791 6010 6052 6435
Size: 555 50 84 135 219 42 383
Fragments arranged by size:
1823 832 647 555 519 383 366 348
238 219 194 135 84 50 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1010 6435
Size: 1010 5425
SfoI GGC'GCC
Cuts at: 0 5589 6435
Size: 5589 846
SmlI C'TyrA_G
Cuts at: 0 978 1868 3434 3952 4423 5334 6435
Size: 978 890 1566 518 471 911 1101
Fragments arranged by size:
1566 1101 978 911 890 518 471
SspI AAT'ATT
Cuts at: 0 183 445 751 4065 6435
Size: 183 262 306 3314 2370
Fragments arranged by size:
3314 2370 306 262 183
StuI AGG'CCT
Cuts at: 0 3421 4451 5998 6435
Size: 3421 1030 1547 437
Fragments arranged by size:
3421 1547 1030 437
StyI C'CwwG_G
Cuts at: 0 1133 1436 1574 2068 6267 6353 6435
Size: 1133 303 138 494 4199 86 82
Fragments arranged by size:
4199 1133 494 303 138 86 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1430 4624 4705 5105 6435
Size: 1430 3194 81 400 1330
Fragments arranged by size:
3194 1430 1330 400 81
TatI w'GTAC_w
Cuts at: 0 3204 3735 3802 6435
Size: 3204 531 67 2633
Fragments arranged by size:
3204 2633 531 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 610 642 1306 1338 6435
Size: 610 32 664 32 5097
Fragments arranged by size:
5097 664 610 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1434 2013 3070 6435
Size: 1434 579 1057 3365
Fragments arranged by size:
3365 1434 1057 579
XhoI C'TCGA_G
Cuts at: 0 978 1868 6435
Size: 978 890 4567
Fragments arranged by size:
4567 978 890
XmnI GAAnn'nnTTC
Cuts at: 0 1422 6435
Size: 1422 5013
ZraI GAC'GTC
Cuts at: 0 69 3303 6435
Size: 69 3234 3132
Fragments arranged by size:
3234 3132 69
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI MmeI MscI MslI MspA1I NarI NcoI NheI
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII
SapI SbfI SexAI SfcI SfiI SfoI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AlfI AscI AsiSI AvrII BglII
BmgBI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstZ17I ClaI CspCI DrdI EcoRI FseI FspAI KpnI
MfeI MluI NaeI NdeI NgoMIV NotI NruI PacI
PmeI PshAI PsiI PvuI RsrII SalI SanDI ScaI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XmaI