Restriction map for
pFN21AE2353
(Linear) (Six-base) MAPSORT of: KIEE2353.seq Check: 184 from: 1 to: 999
KIEE2353.orf
With 182 enzymes: SgfI *
June 2, 2011 17:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 775 999
Size: 210 565 224
Fragments arranged by size:
565 224 210
AflIII A'CryG_T
Cuts at: 0 72 999
Size: 72 927
AlwNI CAG_nnn'CTG
Cuts at: 0 18 999
Size: 18 981
AvaI C'yCGr_G
Cuts at: 0 287 999
Size: 287 712
BanI G'GyrC_C
Cuts at: 0 447 999
Size: 447 552
BanII G_rGCy'C
Cuts at: 0 139 382 999
Size: 139 243 617
Fragments arranged by size:
617 243 139
BglI GCCn_nnn'nGGC
Cuts at: 0 386 999
Size: 386 613
BglII A'GATC_T
Cuts at: 0 95 999
Size: 95 904
Bme1580I G_kGCm'C
Cuts at: 0 126 745 935 999
Size: 126 619 190 64
Fragments arranged by size:
619 190 126 64
BmgBI CAC'GTC
Cuts at: 0 75 999
Size: 75 924
BmrI ACTGGGnnnn_n'
Cuts at: 0 748 894 999
Size: 748 146 105
Fragments arranged by size:
748 146 105
BmtI G_CTAG'C
Cuts at: 0 28 999
Size: 28 971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 823 999
Size: 440 383 176
Fragments arranged by size:
440 383 176
Bpu10I CC'TnA_GC
Cuts at: 0 138 999
Size: 138 861
BsaI GGTCTCn'nnnn_
Cuts at: 0 577 999
Size: 577 422
BsaHI Gr'CG_yC
Cuts at: 0 515 999
Size: 515 484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 832 999
Size: 832 167
BseYI C'CCAG_C
Cuts at: 0 170 640 999
Size: 170 470 359
Fragments arranged by size:
470 359 170
BsmI GAATG_Cn'
Cuts at: 0 366 999
Size: 366 633
BsmBI CGTCTCn'nnnn_
Cuts at: 0 81 999
Size: 81 918
Bsp1286I G_dGCh'C
Cuts at: 0 126 139 382 745 935 999
Size: 126 13 243 363 190 64
Fragments arranged by size:
363 243 190 126 64 13
BsrDI GCAATG_nn'
Cuts at: 0 237 999
Size: 237 762
BsrGI T'GTAC_A
Cuts at: 0 597 999
Size: 597 402
BssSI C'ACGA_G
Cuts at: 0 125 999
Size: 125 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 999
Size: 18 981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 623 999
Size: 623 376
BstYI r'GATC_y
Cuts at: 0 95 999
Size: 95 904
BtsI GCAGTG_nn'
Cuts at: 0 975 999
Size: 975 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 854 999
Size: 854 145
EaeI y'GGCC_r
Cuts at: 0 654 999
Size: 654 345
EarI CTCTTCn'nnn_
Cuts at: 0 35 230 999
Size: 35 195 769
Fragments arranged by size:
769 195 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 440 775 823 999
Size: 210 230 335 48 176
Fragments arranged by size:
335 230 210 176 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 566 999
Size: 240 326 433
Fragments arranged by size:
433 326 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 771 796 803 828 999
Size: 166 32 573 25 7 25 171
Fragments arranged by size:
573 171 166 32 25 25 7
HincII GTy'rAC
Cuts at: 0 111 999
Size: 111 888
MscI TGG'CCA
Cuts at: 0 656 999
Size: 656 343
MslI CAynn'nnrTG
Cuts at: 0 506 999
Size: 506 493
MspA1I CmG'CkG
Cuts at: 0 660 999
Size: 660 339
NheI G'CTAG_C
Cuts at: 0 24 999
Size: 24 975
PflMI CCAn_nnn'nTGG
Cuts at: 0 463 908 999
Size: 463 445 91
Fragments arranged by size:
463 445 91
PfoI T'CCnGG_A
Cuts at: 0 575 800 999
Size: 575 225 199
Fragments arranged by size:
575 225 199
PstI C_TGCA'G
Cuts at: 0 14 999
Size: 14 985
PvuII CAG'CTG
Cuts at: 0 660 999
Size: 660 339
SapI GCTCTTCn'nnn_
Cuts at: 0 35 999
Size: 35 964
SfcI C'TryA_G
Cuts at: 0 10 999
Size: 10 989
StyI C'CwwG_G
Cuts at: 0 215 645 917 935 999
Size: 215 430 272 18 64
Fragments arranged by size:
430 272 215 64 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 696 999
Size: 435 261 303
Fragments arranged by size:
435 303 261
TatI w'GTAC_w
Cuts at: 0 339 597 999
Size: 339 258 402
Fragments arranged by size:
402 339 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 883 915 999
Size: 883 32 84
Fragments arranged by size:
883 84 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 701 999
Size: 701 298
Enzymes that do cut:
AcuI AflIII AlwNI AvaI BanI BanII BglI BglII
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaHI
BseRI BseYI BsmI BsmBI Bsp1286I BsrDI BsrGI BssSI
BstAPI BstXI BstYI BtsI DrdI EaeI EarI Eco57MI
FalI Hin4I HincII MscI MslI MspA1I NheI PflMI
PfoI PstI PvuII SapI SfcI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BlpI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BspEI
BspHI BspMI BsrBI BsrFI BssHII BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI