Restriction map for
pFN21AE2350
(Linear) (Six-base) MAPSORT of: KIEE2350.seq Check: 5371 from: 1 to: 1338
KIEE2350.orf
With 182 enzymes: SgfI *
May 18, 2011 13:28 ..
AccI GT'mk_AC
Cuts at: 0 667 1338
Size: 667 671
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 414 670 982 1267 1338
Size: 277 137 256 312 285 71
Fragments arranged by size:
312 285 277 256 137 71
AleI CACnn'nnGTG
Cuts at: 0 421 925 1338
Size: 421 504 413
Fragments arranged by size:
504 421 413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 869 1338
Size: 869 469
AlwNI CAG_nnn'CTG
Cuts at: 0 1281 1293 1338
Size: 1281 12 45
Fragments arranged by size:
1281 45 12
ApaI G_GGCC'C
Cuts at: 0 1325 1338
Size: 1325 13
ApaLI G'TGCA_C
Cuts at: 0 844 1338
Size: 844 494
BamHI G'GATC_C
Cuts at: 0 169 1338
Size: 169 1169
BanI G'GyrC_C
Cuts at: 0 564 991 1338
Size: 564 427 347
Fragments arranged by size:
564 427 347
BanII G_rGCy'C
Cuts at: 0 8 26 1325 1338
Size: 8 18 1299 13
Fragments arranged by size:
1299 18 13 8
BbeI G_GCGC'C
Cuts at: 0 568 1338
Size: 568 770
BbsI GAAGACnn'nnnn_
Cuts at: 0 480 970 1338
Size: 480 490 368
Fragments arranged by size:
490 480 368
BglI GCCn_nnn'nGGC
Cuts at: 0 64 1338
Size: 64 1274
Bme1580I G_kGCm'C
Cuts at: 0 848 1325 1338
Size: 848 477 13
Fragments arranged by size:
848 477 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 603 663 1338
Size: 369 234 60 675
Fragments arranged by size:
675 369 234 60
BsaHI Gr'CG_yC
Cuts at: 0 565 1338
Size: 565 773
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 126 1108 1243 1338
Size: 121 5 982 135 95
Fragments arranged by size:
982 135 121 95 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 1338
Size: 344 994
BsiEI CG_ry'CG
Cuts at: 0 120 1338
Size: 120 1218
BsiHKAI G_wGCw'C
Cuts at: 0 8 26 355 380 848 1270 1338
Size: 8 18 329 25 468 422 68
Fragments arranged by size:
468 422 329 68 25 18 8
Bsp1286I G_dGCh'C
Cuts at: 0 8 26 355 380 848 1270 1325 1338
Size: 8 18 329 25 468 422 55 13
Fragments arranged by size:
468 422 329 55 25 18 13 8
BspHI T'CATG_A
Cuts at: 0 608 1338
Size: 608 730
BsrBI CCG'CTC
Cuts at: 0 116 1107 1338
Size: 116 991 231
Fragments arranged by size:
991 231 116
BsrDI GCAATG_nn'
Cuts at: 0 306 584 1338
Size: 306 278 754
Fragments arranged by size:
754 306 278
BsrFI r'CCGG_y
Cuts at: 0 119 1338
Size: 119 1219
BssHII G'CGCG_C
Cuts at: 0 96 1338
Size: 96 1242
BssSI C'ACGA_G
Cuts at: 0 735 1338
Size: 735 603
BstBI TT'CG_AA
Cuts at: 0 818 1338
Size: 818 520
BstYI r'GATC_y
Cuts at: 0 169 1338
Size: 169 1169
BtgI C'CryG_G
Cuts at: 0 836 1113 1220 1338
Size: 836 277 107 118
Fragments arranged by size:
836 277 118 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 631 1338
Size: 631 707
EaeI y'GGCC_r
Cuts at: 0 117 464 1043 1152 1209 1338
Size: 117 347 579 109 57 129
Fragments arranged by size:
579 347 129 117 109 57
EagI C'GGCC_G
Cuts at: 0 117 1338
Size: 117 1221
EarI CTCTTCn'nnn_
Cuts at: 0 253 974 1338
Size: 253 721 364
Fragments arranged by size:
721 364 253
EcoICRI GAG'CTC
Cuts at: 0 6 24 1338
Size: 6 18 1314
Fragments arranged by size:
1314 18 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 369 414 603 663 670 982 1267
Size: 277 92 45 189 60 7 312 285
Cuts at: 1267 1338
Size: 71
Fragments arranged by size:
312 285 277 189 92 71 60 45
7
EcoO109I rG'GnC_Cy
Cuts at: 0 948 1321 1338
Size: 948 373 17
Fragments arranged by size:
948 373 17
FseI GG_CCGG'CC
Cuts at: 0 123 1338
Size: 123 1215
HaeII r_GCGC'y
Cuts at: 0 568 1338
Size: 568 770
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 437 469 1338
Size: 437 32 869
Fragments arranged by size:
869 437 32
KasI G'GCGC_C
Cuts at: 0 564 1338
Size: 564 774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1283 1338
Size: 887 396 55
Fragments arranged by size:
887 396 55
MscI TGG'CCA
Cuts at: 0 466 1045 1211 1338
Size: 466 579 166 127
Fragments arranged by size:
579 466 166 127
MslI CAynn'nnrTG
Cuts at: 0 421 925 1338
Size: 421 504 413
Fragments arranged by size:
504 421 413
MspA1I CmG'CkG
Cuts at: 0 195 269 838 1293 1338
Size: 195 74 569 455 45
Fragments arranged by size:
569 455 195 74 45
NaeI GCC'GGC
Cuts at: 0 121 1338
Size: 121 1217
NarI GG'CG_CC
Cuts at: 0 565 1338
Size: 565 773
NcoI C'CATG_G
Cuts at: 0 1113 1220 1338
Size: 1113 107 118
Fragments arranged by size:
1113 118 107
NgoMIV G'CCGG_C
Cuts at: 0 119 1338
Size: 119 1219
PflMI CCAn_nnn'nTGG
Cuts at: 0 804 1035 1338
Size: 804 231 303
Fragments arranged by size:
804 303 231
PfoI T'CCnGG_A
Cuts at: 0 797 1338
Size: 797 541
PpuMI rG'GwC_Cy
Cuts at: 0 948 1338
Size: 948 390
PspOMI G'GGCC_C
Cuts at: 0 1321 1338
Size: 1321 17
PstI C_TGCA'G
Cuts at: 0 252 371 445 1286 1338
Size: 252 119 74 841 52
Fragments arranged by size:
841 252 119 74 52
PvuII CAG'CTG
Cuts at: 0 1293 1338
Size: 1293 45
SacI G_AGCT'C
Cuts at: 0 8 26 1338
Size: 8 18 1312
Fragments arranged by size:
1312 18 8
SacII CC_GC'GG
Cuts at: 0 839 1338
Size: 839 499
SapI GCTCTTCn'nnn_
Cuts at: 0 253 1338
Size: 253 1085
SfcI C'TryA_G
Cuts at: 0 248 367 441 1282 1338
Size: 248 119 74 841 56
Fragments arranged by size:
841 248 119 74 56
SfoI GGC'GCC
Cuts at: 0 566 1338
Size: 566 772
StyI C'CwwG_G
Cuts at: 0 356 893 1040 1113 1220 1338
Size: 356 537 147 73 107 118
Fragments arranged by size:
537 356 147 118 107 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 1338
Size: 247 1091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 139 402 1338
Size: 139 263 936
Fragments arranged by size:
936 263 139
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApaLI BamHI
BanI BanII BbeI BbsI BglI Bme1580I BpmI BsaHI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstYI BtgI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SapI SfcI SfoI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI
BspMI BsrGI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EciI EcoNI EcoRI
EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI