Restriction map for
pFN21AE2331
(Linear) (Six-base) MAPSORT of: KIEE2331.seq Check: 3615 from: 1 to: 2181
KIEE2331.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 701 1434 2181
Size: 701 733 747
Fragments arranged by size:
747 733 701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 775 1442 2181
Size: 331 444 667 739
Fragments arranged by size:
739 667 444 331
AflIII A'CryG_T
Cuts at: 0 831 2181
Size: 831 1350
AhdI GACnn_n'nnGTC
Cuts at: 0 462 2181
Size: 462 1719
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 1524 2181
Size: 1386 138 657
Fragments arranged by size:
1386 657 138
AseI AT'TA_AT
Cuts at: 0 740 1085 2181
Size: 740 345 1096
Fragments arranged by size:
1096 740 345
AvaI C'yCGr_G
Cuts at: 0 282 332 2017 2181
Size: 282 50 1685 164
Fragments arranged by size:
1685 282 164 50
BanI G'GyrC_C
Cuts at: 0 46 1885 2181
Size: 46 1839 296
Fragments arranged by size:
1839 296 46
BanII G_rGCy'C
Cuts at: 0 846 2150 2181
Size: 846 1304 31
Fragments arranged by size:
1304 846 31
BbeI G_GCGC'C
Cuts at: 0 50 2181
Size: 50 2131
BbsI GAAGACnn'nnnn_
Cuts at: 0 923 1223 1430 2181
Size: 923 300 207 751
Fragments arranged by size:
923 751 300 207
BciVI GTATCCnnnnn_n'
Cuts at: 0 513 1387 2181
Size: 513 874 794
Fragments arranged by size:
874 794 513
BclI T'GATC_A
Cuts at: 0 72 1640 2181
Size: 72 1568 541
Fragments arranged by size:
1568 541 72
BglI GCCn_nnn'nGGC
Cuts at: 0 850 2181
Size: 850 1331
BglII A'GATC_T
Cuts at: 0 1277 2181
Size: 1277 904
BlpI GC'TnA_GC
Cuts at: 0 2068 2181
Size: 2068 113
Bme1580I G_kGCm'C
Cuts at: 0 1799 1890 2181
Size: 1799 91 291
Fragments arranged by size:
1799 291 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 224 2181
Size: 136 88 1957
Fragments arranged by size:
1957 136 88
Bpu10I CC'TnA_GC
Cuts at: 0 1525 2181
Size: 1525 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 2181
Size: 1219 962
BsaI GGTCTCn'nnnn_
Cuts at: 0 128 1300 2181
Size: 128 1172 881
Fragments arranged by size:
1172 881 128
BsaBI GATnn'nnATC
Cuts at: 0 670 2181
Size: 670 1511
BsaHI Gr'CG_yC
Cuts at: 0 47 2181
Size: 47 2134
BsaWI w'CCGG_w
Cuts at: 0 1187 2181
Size: 1187 994
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 222 252 1104 1134 2181
Size: 222 30 852 30 1047
Fragments arranged by size:
1047 852 222 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 786 1372 2181
Size: 373 413 586 809
Fragments arranged by size:
809 586 413 373
BseYI C'CCAG_C
Cuts at: 0 254 845 2149 2181
Size: 254 591 1304 32
Fragments arranged by size:
1304 591 254 32
BsiHKAI G_wGCw'C
Cuts at: 0 629 899 1473 2181
Size: 629 270 574 708
Fragments arranged by size:
708 629 574 270
BsmI GAATG_Cn'
Cuts at: 0 2013 2181
Size: 2013 168
Bsp1286I G_dGCh'C
Cuts at: 0 629 846 899 1473 1799 1890 2150 2181
Size: 629 217 53 574 326 91 260 31
Fragments arranged by size:
629 574 326 260 217 91 53 31
BspEI T'CCGG_A
Cuts at: 0 1187 2181
Size: 1187 994
BspHI T'CATG_A
Cuts at: 0 437 2181
Size: 437 1744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 1318 1434 1449 1687 2181
Size: 701 617 116 15 238 494
Fragments arranged by size:
701 617 494 238 116 15
BsrBI CCG'CTC
Cuts at: 0 404 2181
Size: 404 1777
BsrDI GCAATG_nn'
Cuts at: 0 204 2181
Size: 204 1977
BsrFI r'CCGG_y
Cuts at: 0 1824 2181
Size: 1824 357
BsrGI T'GTAC_A
Cuts at: 0 1052 2181
Size: 1052 1129
BstBI TT'CG_AA
Cuts at: 0 981 2181
Size: 981 1200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 2181
Size: 63 2118
BstYI r'GATC_y
Cuts at: 0 1277 1348 1538 2114 2181
Size: 1277 71 190 576 67
Fragments arranged by size:
1277 576 190 71 67
Bsu36I CC'TnA_GG
Cuts at: 0 1784 2181
Size: 1784 397
BtgI C'CryG_G
Cuts at: 0 11 501 1103 1844 2181
Size: 11 490 602 741 337
Fragments arranged by size:
741 602 490 337 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1947 2181
Size: 1947 234
BtsI GCAGTG_nn'
Cuts at: 0 740 1029 2181
Size: 740 289 1152
Fragments arranged by size:
1152 740 289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1005 1040 2181
Size: 1005 35 1141
Fragments arranged by size:
1141 1005 35
EaeI y'GGCC_r
Cuts at: 0 29 40 320 605 675 1239 2181
Size: 29 11 280 285 70 564 942
Fragments arranged by size:
942 564 285 280 70 29 11
EarI CTCTTCn'nnn_
Cuts at: 0 1769 2181
Size: 1769 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1540 1719 2077 2120 2181
Size: 1540 179 358 43 61
Fragments arranged by size:
1540 358 179 61 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 224 331 775 1442 2181
Size: 136 88 107 444 667 739
Fragments arranged by size:
739 667 444 136 107 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1683 2181
Size: 1683 498
EcoO109I rG'GnC_Cy
Cuts at: 0 1676 2181
Size: 1676 505
EcoRV GAT'ATC
Cuts at: 0 1836 2181
Size: 1836 345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 465 1007 1906 2181
Size: 465 542 899 275
Fragments arranged by size:
899 542 465 275
HaeII r_GCGC'y
Cuts at: 0 50 2181
Size: 50 2131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 361 393 448 480 988 1020
Size: 106 32 223 32 55 32 508 32
Cuts at: 1020 1122 1154 1570 1602 2181
Size: 102 32 416 32 579
Fragments arranged by size:
579 508 416 223 106 102 55 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1095 2181
Size: 1095 1086
HindIII A'AGCT_T
Cuts at: 0 1072 2181
Size: 1072 1109
KasI G'GCGC_C
Cuts at: 0 46 2181
Size: 46 2135
MscI TGG'CCA
Cuts at: 0 31 322 607 2181
Size: 31 291 285 1574
Fragments arranged by size:
1574 291 285 31
MslI CAynn'nnrTG
Cuts at: 0 391 1175 2181
Size: 391 784 1006
Fragments arranged by size:
1006 784 391
MspA1I CmG'CkG
Cuts at: 0 13 597 849 879 2181
Size: 13 584 252 30 1302
Fragments arranged by size:
1302 584 252 30 13
NarI GG'CG_CC
Cuts at: 0 47 2181
Size: 47 2134
NcoI C'CATG_G
Cuts at: 0 501 1103 2181
Size: 501 602 1078
Fragments arranged by size:
1078 602 501
NdeI CA'TA_TG
Cuts at: 0 1871 2181
Size: 1871 310
NspI r_CATG'y
Cuts at: 0 160 462 835 1152 2181
Size: 160 302 373 317 1029
Fragments arranged by size:
1029 373 317 302 160
PciI A'CATG_T
Cuts at: 0 831 2181
Size: 831 1350
PflMI CCAn_nnn'nTGG
Cuts at: 0 38 654 2181
Size: 38 616 1527
Fragments arranged by size:
1527 616 38
PfoI T'CCnGG_A
Cuts at: 0 911 2093 2181
Size: 911 1182 88
Fragments arranged by size:
1182 911 88
PpuMI rG'GwC_Cy
Cuts at: 0 1676 2181
Size: 1676 505
PsiI TTA'TAA
Cuts at: 0 361 1067 2181
Size: 361 706 1114
Fragments arranged by size:
1114 706 361
PspXI vC'TCGA_Gb
Cuts at: 0 282 2181
Size: 282 1899
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1513 1545 2181
Size: 1513 32 636
Fragments arranged by size:
1513 636 32
PstI C_TGCA'G
Cuts at: 0 596 1520 2181
Size: 596 924 661
Fragments arranged by size:
924 661 596
PvuII CAG'CTG
Cuts at: 0 879 2181
Size: 879 1302
SacII CC_GC'GG
Cuts at: 0 14 2181
Size: 14 2167
ScaI AGT'ACT
Cuts at: 0 1984 2181
Size: 1984 197
SfcI C'TryA_G
Cuts at: 0 592 1516 2181
Size: 592 924 665
Fragments arranged by size:
924 665 592
SfoI GGC'GCC
Cuts at: 0 48 2181
Size: 48 2133
SmaI CCC'GGG
Cuts at: 0 334 2181
Size: 334 1847
SmlI C'TyrA_G
Cuts at: 0 282 1198 2181
Size: 282 916 983
Fragments arranged by size:
983 916 282
SphI G_CATG'C
Cuts at: 0 160 2181
Size: 160 2021
SspI AAT'ATT
Cuts at: 0 142 792 2181
Size: 142 650 1389
Fragments arranged by size:
1389 650 142
StyI C'CwwG_G
Cuts at: 0 501 602 1103 2141 2181
Size: 501 101 501 1038 40
Fragments arranged by size:
1038 501 501 101 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 1354 2181
Size: 107 1247 827
Fragments arranged by size:
1247 827 107
TatI w'GTAC_w
Cuts at: 0 1052 1154 1982 2181
Size: 1052 102 828 199
Fragments arranged by size:
1052 828 199 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 821 853 1104 1136 1333 1365 2181
Size: 821 32 251 32 197 32 816
Fragments arranged by size:
821 816 251 197 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 19 2164 2181
Size: 19 2145 17
Fragments arranged by size:
2145 19 17
XhoI C'TCGA_G
Cuts at: 0 282 2181
Size: 282 1899
XmaI C'CCGG_G
Cuts at: 0 332 2181
Size: 332 1849
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI AseI AvaI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII KasI MscI MslI MspA1I NarI NcoI NdeI
NspI PciI PflMI PfoI PpuMI PsiI PspXI PsrI
PstI PvuII SacII ScaI SfcI SfoI SmaI SmlI
SphI SspI StyI TaqII TatI TstI Tth111I XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII
BaeI BamHI BbvCI BcgI BfrBI BmgBI BmrI BmtI
BplI BsaAI BsgI BsiEI BsiWI BsmBI BssHII BssSI
BstAPI BstEII BstZ17I ClaI DraI DraIII DrdI EagI
EcoICRI EcoRI FseI FspI FspAI HpaI KpnI MfeI
MluI MmeI NaeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PspOMI PvuI
RsrII SacI SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI StuI SwaI XbaI
XcmI XmnI ZraI