Restriction map for
pFN21AE2319
(Linear) (Six-base) MAPSORT of: KIEE2319.seq Check: 9189 from: 1 to: 999
KIEE2319.orf
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 999
Size: 748 251
AleI CACnn'nnGTG
Cuts at: 0 620 999
Size: 620 379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 27 45 999
Size: 13 14 18 954
Fragments arranged by size:
954 18 14 13
AlwNI CAG_nnn'CTG
Cuts at: 0 63 486 870 999
Size: 63 423 384 129
Fragments arranged by size:
423 384 129 63
ApaI G_GGCC'C
Cuts at: 0 768 999
Size: 768 231
ApoI r'AATT_y
Cuts at: 0 493 721 999
Size: 493 228 278
Fragments arranged by size:
493 278 228
BanII G_rGCy'C
Cuts at: 0 768 999
Size: 768 231
BciVI GTATCCnnnnn_n'
Cuts at: 0 292 999
Size: 292 707
BglI GCCn_nnn'nGGC
Cuts at: 0 259 750 999
Size: 259 491 249
Fragments arranged by size:
491 259 249
Bme1580I G_kGCm'C
Cuts at: 0 768 999
Size: 768 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 999
Size: 157 842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 626 859 999
Size: 626 233 140
Fragments arranged by size:
626 233 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 638 770 792 999
Size: 538 100 132 22 207
Fragments arranged by size:
538 207 132 100 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 514 999
Size: 514 485
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 999
Size: 25 974
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 999
Size: 696 303
BseYI C'CCAG_C
Cuts at: 0 72 945 999
Size: 72 873 54
Fragments arranged by size:
873 72 54
BsiEI CG_ry'CG
Cuts at: 0 259 999
Size: 259 740
Bsp1286I G_dGCh'C
Cuts at: 0 768 999
Size: 768 231
BsrBI CCG'CTC
Cuts at: 0 39 184 806 980 999
Size: 39 145 622 174 19
Fragments arranged by size:
622 174 145 39 19
BsrFI r'CCGG_y
Cuts at: 0 99 258 999
Size: 99 159 741
Fragments arranged by size:
741 159 99
BssSI C'ACGA_G
Cuts at: 0 620 999
Size: 620 379
BstAPI GCAn_nnn'nTGC
Cuts at: 0 870 999
Size: 870 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 403 816 999
Size: 403 413 183
Fragments arranged by size:
413 403 183
Bsu36I CC'TnA_GG
Cuts at: 0 64 999
Size: 64 935
BtgI C'CryG_G
Cuts at: 0 396 402 999
Size: 396 6 597
Fragments arranged by size:
597 396 6
EaeI y'GGCC_r
Cuts at: 0 399 999
Size: 399 600
EarI CTCTTCn'nnn_
Cuts at: 0 421 999
Size: 421 578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 835 999
Size: 835 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 638 748 770 792 999
Size: 538 100 110 22 22 207
Fragments arranged by size:
538 207 110 100 22 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 600 999
Size: 600 399
EcoO109I rG'GnC_Cy
Cuts at: 0 68 764 765 838 999
Size: 68 696 1 73 161
Fragments arranged by size:
696 161 73 68 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 327 999
Size: 327 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 999
Size: 460 32 507
Fragments arranged by size:
507 460 32
MfeI C'AATT_G
Cuts at: 0 464 999
Size: 464 535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 999
Size: 276 723
MscI TGG'CCA
Cuts at: 0 401 999
Size: 401 598
MslI CAynn'nnrTG
Cuts at: 0 401 620 999
Size: 401 219 379
Fragments arranged by size:
401 379 219
MspA1I CmG'CkG
Cuts at: 0 649 999
Size: 649 350
NaeI GCC'GGC
Cuts at: 0 101 999
Size: 101 898
NcoI C'CATG_G
Cuts at: 0 396 402 999
Size: 396 6 597
Fragments arranged by size:
597 396 6
NgoMIV G'CCGG_C
Cuts at: 0 99 999
Size: 99 900
PasI CC'CwG_GG
Cuts at: 0 934 999
Size: 934 65
PfoI T'CCnGG_A
Cuts at: 0 32 362 999
Size: 32 330 637
Fragments arranged by size:
637 330 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 999
Size: 27 972
PpuMI rG'GwC_Cy
Cuts at: 0 838 999
Size: 838 161
PshAI GACnn'nnGTC
Cuts at: 0 526 999
Size: 526 473
PspOMI G'GGCC_C
Cuts at: 0 764 999
Size: 764 235
PstI C_TGCA'G
Cuts at: 0 633 999
Size: 633 366
SfcI C'TryA_G
Cuts at: 0 115 629 999
Size: 115 514 370
Fragments arranged by size:
514 370 115
SpeI A'CTAG_T
Cuts at: 0 193 999
Size: 193 806
StyI C'CwwG_G
Cuts at: 0 164 396 402 748 999
Size: 164 232 6 346 251
Fragments arranged by size:
346 251 232 164 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 485 687 731 809 999
Size: 485 202 44 78 190
Fragments arranged by size:
485 202 190 78 44
TatI w'GTAC_w
Cuts at: 0 315 999
Size: 315 684
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 344 376 999
Size: 344 32 623
Fragments arranged by size:
623 344 32
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApoI BanII BciVI
BglI Bme1580I BmrI BplI BpmI BsaI BsaXI BseRI
BseYI BsiEI Bsp1286I BsrBI BsrFI BssSI BstAPI BstXI
Bsu36I BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI Hin4I MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PasI PfoI PpiI PpuMI PshAI PspOMI
PstI SfcI SpeI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsgI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI
BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI