Restriction map for
pFN21AE2318
(Linear) (Six-base) MAPSORT of: KIEE2318.seq Check: 3940 from: 1 to: 5940
KIEE2318.orf
With 182 enzymes: SgfI *
June 13, 2011 11:57 ..
Acc65I G'GTAC_C
Cuts at: 0 3285 5940
Size: 3285 2655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 730 1343 1532 1680 2425 2922 3377
Size: 294 436 613 189 148 745 497 455
Cuts at: 3377 3416 5289 5940
Size: 39 1873 651
Fragments arranged by size:
1873 745 651 613 497 455 436 294
189 148 39
AflII C'TTAA_G
Cuts at: 0 1315 3909 5940
Size: 1315 2594 2031
Fragments arranged by size:
2594 2031 1315
AflIII A'CryG_T
Cuts at: 0 488 2346 3248 4316 5051 5276 5940
Size: 488 1858 902 1068 735 225 664
Fragments arranged by size:
1858 1068 902 735 664 488 225
AgeI A'CCGG_T
Cuts at: 0 3145 5940
Size: 3145 2795
AhdI GACnn_n'nnGTC
Cuts at: 0 3249 5940
Size: 3249 2691
AleI CACnn'nnGTG
Cuts at: 0 3146 5940
Size: 3146 2794
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 5599 5631 5940
Size: 475 32 5092 32 309
Fragments arranged by size:
5092 475 309 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 31 946 1369 1638 5940
Size: 31 915 423 269 4302
Fragments arranged by size:
4302 915 423 269 31
ApaI G_GGCC'C
Cuts at: 0 1821 5940
Size: 1821 4119
ApaLI G'TGCA_C
Cuts at: 0 417 3043 5940
Size: 417 2626 2897
Fragments arranged by size:
2897 2626 417
ApoI r'AATT_y
Cuts at: 0 380 930 1291 1615 2173 2381 2607 5940
Size: 380 550 361 324 558 208 226 3333
Fragments arranged by size:
3333 558 550 380 361 324 226 208
AvaI C'yCGr_G
Cuts at: 0 1534 1728 5940
Size: 1534 194 4212
Fragments arranged by size:
4212 1534 194
AvrII C'CTAG_G
Cuts at: 0 636 5940
Size: 636 5304
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 999 1032 5940
Size: 999 33 4908
Fragments arranged by size:
4908 999 33
BamHI G'GATC_C
Cuts at: 0 1530 2421 3351 3465 3554 5940
Size: 1530 891 930 114 89 2386
Fragments arranged by size:
2386 1530 930 891 114 89
BanI G'GyrC_C
Cuts at: 0 724 886 2617 3285 3637 3868 3964 4897
Size: 724 162 1731 668 352 231 96 933
Cuts at: 4897 5940
Size: 1043
Fragments arranged by size:
1731 1043 933 724 668 352 231 162
96
BanII G_rGCy'C
Cuts at: 0 1417 1429 1735 1821 2935 4489 5486 5940
Size: 1417 12 306 86 1114 1554 997 454
Fragments arranged by size:
1554 1417 1114 997 454 306 86 12
BbeI G_GCGC'C
Cuts at: 0 3641 3968 4901 5940
Size: 3641 327 933 1039
Fragments arranged by size:
3641 1039 933 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 718 2544 2649 3705 5940
Size: 718 1826 105 1056 2235
Fragments arranged by size:
2235 1826 1056 718 105
BbvCI CC'TCA_GC
Cuts at: 0 5109 5940
Size: 5109 831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2780 2814 5374 5408 5940
Size: 2780 34 2560 34 532
Fragments arranged by size:
2780 2560 532 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1000 4198 5940
Size: 1000 3198 1742
Fragments arranged by size:
3198 1742 1000
BclI T'GATC_A
Cuts at: 0 407 4889 5940
Size: 407 4482 1051
Fragments arranged by size:
4482 1051 407
BfrBI ATG'CAT
Cuts at: 0 2757 5940
Size: 2757 3183
BglI GCCn_nnn'nGGC
Cuts at: 0 1081 5940
Size: 1081 4859
BglII A'GATC_T
Cuts at: 0 2993 5369 5940
Size: 2993 2376 571
Fragments arranged by size:
2993 2376 571
BlpI GC'TnA_GC
Cuts at: 0 1642 3628 5940
Size: 1642 1986 2312
Fragments arranged by size:
2312 1986 1642
Bme1580I G_kGCm'C
Cuts at: 0 421 729 1821 2158 2622 3047 5465 5940
Size: 421 308 1092 337 464 425 2418 475
Fragments arranged by size:
2418 1092 475 464 425 421 337 308
BmgBI CAC'GTC
Cuts at: 0 2508 5940
Size: 2508 3432
BmrI ACTGGGnnnn_n'
Cuts at: 0 5394 5940
Size: 5394 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1285 2974 3754 5317 5794 5940
Size: 262 1023 1689 780 1563 477 146
Fragments arranged by size:
1689 1563 1023 780 477 262 146
Bpu10I CC'TnA_GC
Cuts at: 0 2929 5109 5940
Size: 2929 2180 831
Fragments arranged by size:
2929 2180 831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 3730 5940
Size: 85 3645 2210
Fragments arranged by size:
3645 2210 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 1718 2809 3595 3897 5350 5940
Size: 1718 1091 786 302 1453 590
Fragments arranged by size:
1718 1453 1091 786 590 302
BsaBI GATnn'nnATC
Cuts at: 0 3007 4249 4591 5940
Size: 3007 1242 342 1349
Fragments arranged by size:
3007 1349 1242 342
BsaHI Gr'CG_yC
Cuts at: 0 1983 3638 3965 4898 5940
Size: 1983 1655 327 933 1042
Fragments arranged by size:
1983 1655 1042 933 327
BsaWI w'CCGG_w
Cuts at: 0 3145 3161 5940
Size: 3145 16 2779
Fragments arranged by size:
3145 2779 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1717 1747 3373 3403 5940
Size: 475 30 1212 30 1626 30 2537
Fragments arranged by size:
2537 1626 1212 475 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1282 1534 1789 1866 3381 5698 5940
Size: 1282 252 255 77 1515 2317 242
Fragments arranged by size:
2317 1515 1282 255 252 242 77
BseYI C'CCAG_C
Cuts at: 0 988 1070 1891 4482 5940
Size: 988 82 821 2591 1458
Fragments arranged by size:
2591 1458 988 821 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2963 3170 5348 5940
Size: 2963 207 2178 592
Fragments arranged by size:
2963 2178 592 207
BsiHKAI G_wGCw'C
Cuts at: 0 421 1429 1763 2321 2935 3047 3590 4712
Size: 421 1008 334 558 614 112 543 1122
Cuts at: 4712 5486 5812 5940
Size: 774 326 128
Fragments arranged by size:
1122 1008 774 614 558 543 421 334
326 128 112
BsmI GAATG_Cn'
Cuts at: 0 3957 5940
Size: 3957 1983
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1990 5940
Size: 1990 3950
Bsp1286I G_dGCh'C
Cuts at: 0 421 729 1417 1429 1735 1763 1821 2158
Size: 421 308 688 12 306 28 58 337
Cuts at: 2158 2321 2622 2935 3047 3590 4489 4712 5465
Size: 163 301 313 112 543 899 223 753
Cuts at: 5465 5486 5812 5940
Size: 21 326 128
Fragments arranged by size:
899 753 688 543 421 337 326 313
308 306 301 223 163 128 112 58
28 21 12
BspHI T'CATG_A
Cuts at: 0 443 605 1625 3617 3980 4439 4583 5940
Size: 443 162 1020 1992 363 459 144 1357
Fragments arranged by size:
1992 1357 1020 459 443 363 162 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3577 5940
Size: 3577 2363
BsrBI CCG'CTC
Cuts at: 0 1874 5513 5613 5940
Size: 1874 3639 100 327
Fragments arranged by size:
3639 1874 327 100
BsrDI GCAATG_nn'
Cuts at: 0 83 530 648 1000 1307 2318 3195 3290
Size: 83 447 118 352 307 1011 877 95
Cuts at: 3290 5282 5940
Size: 1992 658
Fragments arranged by size:
1992 1011 877 658 447 352 307 118
95 83
BsrFI r'CCGG_y
Cuts at: 0 3145 5940
Size: 3145 2795
BsrGI T'GTAC_A
Cuts at: 0 335 4319 5279 5940
Size: 335 3984 960 661
Fragments arranged by size:
3984 960 661 335
BssSI C'ACGA_G
Cuts at: 0 5032 5940
Size: 5032 908
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1342 5087 5431 5528 5940
Size: 1342 3745 344 97 412
Fragments arranged by size:
3745 1342 412 344 97
BstBI TT'CG_AA
Cuts at: 0 831 5940
Size: 831 5109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 584 3166 3184 3297 5295 5940
Size: 584 2582 18 113 1998 645
Fragments arranged by size:
2582 1998 645 584 113 18
BstYI r'GATC_y
Cuts at: 0 1530 2035 2421 2993 3351 3465 3554 5369
Size: 1530 505 386 572 358 114 89 1815
Cuts at: 5369 5496 5940
Size: 127 444
Fragments arranged by size:
1815 1530 572 505 444 386 358 127
114 89
Bsu36I CC'TnA_GG
Cuts at: 0 3452 3883 5940
Size: 3452 431 2057
Fragments arranged by size:
3452 2057 431
BtgI C'CryG_G
Cuts at: 0 577 1232 2051 2417 2669 5940
Size: 577 655 819 366 252 3271
Fragments arranged by size:
3271 819 655 577 366 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1363 5472 5940
Size: 1363 4109 468
Fragments arranged by size:
4109 1363 468
BtsI GCAGTG_nn'
Cuts at: 0 1636 3079 3639 5526 5665 5702 5940
Size: 1636 1443 560 1887 139 37 238
Fragments arranged by size:
1887 1636 1443 560 238 139 37
ClaI AT'CG_AT
Cuts at: 0 5045 5940
Size: 5045 895
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1991 2026 4601 4636 5940
Size: 1991 35 2575 35 1304
Fragments arranged by size:
2575 1991 1304 35 35
DraI TTT'AAA
Cuts at: 0 1296 5940
Size: 1296 4644
EaeI y'GGCC_r
Cuts at: 0 1229 1352 2561 2645 5940
Size: 1229 123 1209 84 3295
Fragments arranged by size:
3295 1229 1209 123 84
EarI CTCTTCn'nnn_
Cuts at: 0 181 313 902 1325 1434 1483 1807 2132
Size: 181 132 589 423 109 49 324 325
Cuts at: 2132 3425 4978 5603 5940
Size: 1293 1553 625 337
Fragments arranged by size:
1553 1293 625 589 423 337 325 324
181 132 109 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2091 5940
Size: 2091 3849
EcoICRI GAG'CTC
Cuts at: 0 1427 2933 5484 5940
Size: 1427 1506 2551 456
Fragments arranged by size:
2551 1506 1427 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 294 730 1285 1343 1532 1680 2425
Size: 262 32 436 555 58 189 148 745
Cuts at: 2425 2922 2974 3377 3416 3754 5289 5317 5794
Size: 497 52 403 39 338 1535 28 477
Cuts at: 5794 5940
Size: 146
Fragments arranged by size:
1535 745 555 497 477 436 403 338
262 189 148 146 58 52 39 32
28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1993 5170 5940
Size: 1993 3177 770
Fragments arranged by size:
3177 1993 770
EcoO109I rG'GnC_Cy
Cuts at: 0 28 573 1708 2029 3435 3887 5562 5940
Size: 28 545 1135 321 1406 452 1675 378
Fragments arranged by size:
1675 1406 1135 545 452 378 321 28
EcoRV GAT'ATC
Cuts at: 0 3414 5940
Size: 3414 2526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4200 5940
Size: 4200 1740
HaeII r_GCGC'y
Cuts at: 0 1830 3641 3968 4901 5940
Size: 1830 1811 327 933 1039
Fragments arranged by size:
1830 1811 1039 933 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1717 1749 2456 2488 3235 3267 3373 3405
Size: 1717 32 707 32 747 32 106 32
Cuts at: 3405 4617 4649 5502 5534 5674 5706 5940
Size: 1212 32 853 32 140 32 234
Fragments arranged by size:
1717 1212 853 747 707 234 140 106
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3522 4038 4110 5940
Size: 3522 516 72 1830
Fragments arranged by size:
3522 1830 516 72
HindIII A'AGCT_T
Cuts at: 0 4132 5940
Size: 4132 1808
HpaI GTT'AAC
Cuts at: 0 4038 5940
Size: 4038 1902
KasI G'GCGC_C
Cuts at: 0 3637 3964 4897 5940
Size: 3637 327 933 1043
Fragments arranged by size:
3637 1043 933 327
KpnI G_GTAC'C
Cuts at: 0 3289 5940
Size: 3289 2651
MfeI C'AATT_G
Cuts at: 0 716 1129 5940
Size: 716 413 4811
Fragments arranged by size:
4811 716 413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1286 2428 3329 5564 5940
Size: 1286 1142 901 2235 376
Fragments arranged by size:
2235 1286 1142 901 376
MscI TGG'CCA
Cuts at: 0 1231 1354 2563 2647 5940
Size: 1231 123 1209 84 3293
Fragments arranged by size:
3293 1231 1209 123 84
MslI CAynn'nnrTG
Cuts at: 0 607 1186 2416 2756 2836 3146 3164 3295
Size: 607 579 1230 340 80 310 18 131
Cuts at: 3295 3616 3985 4438 5491 5940
Size: 321 369 453 1053 449
Fragments arranged by size:
1230 1053 607 579 453 449 369 340
321 310 131 80 18
MspA1I CmG'CkG
Cuts at: 0 813 1844 1853 2392 3203 3501 4264 5113
Size: 813 1031 9 539 811 298 763 849
Cuts at: 5113 5877 5940
Size: 764 63
Fragments arranged by size:
1031 849 813 811 764 763 539 298
63 9
NarI GG'CG_CC
Cuts at: 0 3638 3965 4898 5940
Size: 3638 327 933 1042
Fragments arranged by size:
3638 1042 933 327
NcoI C'CATG_G
Cuts at: 0 577 1232 2051 2417 5940
Size: 577 655 819 366 3523
Fragments arranged by size:
3523 819 655 577 366
NdeI CA'TA_TG
Cuts at: 0 2753 5022 5940
Size: 2753 2269 918
Fragments arranged by size:
2753 2269 918
NsiI A_TGCA'T
Cuts at: 0 2759 5940
Size: 2759 3181
NspI r_CATG'y
Cuts at: 0 954 2350 2589 3252 4320 5055 5280 5940
Size: 954 1396 239 663 1068 735 225 660
Fragments arranged by size:
1396 1068 954 735 663 660 239 225
PasI CC'CwG_GG
Cuts at: 0 2622 3849 5182 5940
Size: 2622 1227 1333 758
Fragments arranged by size:
2622 1333 1227 758
PciI A'CATG_T
Cuts at: 0 2346 3248 4316 5051 5276 5940
Size: 2346 902 1068 735 225 664
Fragments arranged by size:
2346 1068 902 735 664 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 225 2570 3788 4751 5188 5532 5940
Size: 225 2345 1218 963 437 344 408
Fragments arranged by size:
2345 1218 963 437 408 344 225
PfoI T'CCnGG_A
Cuts at: 0 3692 3731 4273 5294 5940
Size: 3692 39 542 1021 646
Fragments arranged by size:
3692 1021 646 542 39
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 950 982 1729 1761 5940
Size: 475 32 443 32 747 32 4179
Fragments arranged by size:
4179 747 475 443 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 573 1708 3435 3887 5562 5940
Size: 573 1135 1727 452 1675 378
Fragments arranged by size:
1727 1675 1135 573 452 378
PspOMI G'GGCC_C
Cuts at: 0 1817 5940
Size: 1817 4123
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4645 4677 5940
Size: 4645 32 1263
Fragments arranged by size:
4645 1263 32
PstI C_TGCA'G
Cuts at: 0 812 5164 5697 5708 5940
Size: 812 4352 533 11 232
Fragments arranged by size:
4352 812 533 232 11
PvuII CAG'CTG
Cuts at: 0 813 4264 5113 5940
Size: 813 3451 849 827
Fragments arranged by size:
3451 849 827 813
SacI G_AGCT'C
Cuts at: 0 1429 2935 5486 5940
Size: 1429 1506 2551 454
Fragments arranged by size:
2551 1506 1429 454
SanDI GG'GwC_CC
Cuts at: 0 573 5940
Size: 573 5367
SapI GCTCTTCn'nnn_
Cuts at: 0 1434 4978 5603 5940
Size: 1434 3544 625 337
Fragments arranged by size:
3544 1434 625 337
ScaI AGT'ACT
Cuts at: 0 4462 5413 5940
Size: 4462 951 527
Fragments arranged by size:
4462 951 527
SexAI A'CCwGG_T
Cuts at: 0 4673 5940
Size: 4673 1267
SfcI C'TryA_G
Cuts at: 0 808 1839 1848 1857 2357 4265 5160 5693
Size: 808 1031 9 9 500 1908 895 533
Cuts at: 5693 5704 5819 5940
Size: 11 115 121
Fragments arranged by size:
1908 1031 895 808 533 500 121 115
11 9 9
SfoI GGC'GCC
Cuts at: 0 3639 3966 4899 5940
Size: 3639 327 933 1041
Fragments arranged by size:
3639 1041 933 327
SgrAI Cr'CCGG_yG
Cuts at: 0 3145 5940
Size: 3145 2795
SmaI CCC'GGG
Cuts at: 0 1730 5940
Size: 1730 4210
SmlI C'TyrA_G
Cuts at: 0 100 1315 3745 3909 5940
Size: 100 1215 2430 164 2031
Fragments arranged by size:
2430 2031 1215 164 100
SpeI A'CTAG_T
Cuts at: 0 519 2121 5940
Size: 519 1602 3819
Fragments arranged by size:
3819 1602 519
SphI G_CATG'C
Cuts at: 0 954 5940
Size: 954 4986
StuI AGG'CCT
Cuts at: 0 224 707 1582 5940
Size: 224 483 875 4358
Fragments arranged by size:
4358 875 483 224
StyI C'CwwG_G
Cuts at: 0 219 577 636 1232 2051 2417 3956 4533
Size: 219 358 59 596 819 366 1539 577
Cuts at: 4533 5441 5940
Size: 908 499
Fragments arranged by size:
1539 908 819 596 577 499 366 358
219 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 2605 4459 5195 5413 5940
Size: 712 1893 1854 736 218 527
Fragments arranged by size:
1893 1854 736 712 527 218
TatI w'GTAC_w
Cuts at: 0 335 485 3227 4319 4460 5279 5411 5940
Size: 335 150 2742 1092 141 819 132 529
Fragments arranged by size:
2742 1092 819 529 335 150 141 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2597 2629 5940
Size: 2597 32 3311
Fragments arranged by size:
3311 2597 32
Tth111I GACn'n_nGTC
Cuts at: 0 3709 5940
Size: 3709 2231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4479 5562 5940
Size: 4479 1083 378
Fragments arranged by size:
4479 1083 378
XmaI C'CCGG_G
Cuts at: 0 1728 5940
Size: 1728 4212
XmnI GAAnn'nnTTC
Cuts at: 0 1744 4372 4417 4759 5940
Size: 1744 2628 45 342 1181
Fragments arranged by size:
2628 1744 1181 342 45
Enzymes that do cut:
Acc65I AcuI AflII AflIII AgeI AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI
NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PsrI PstI PvuII SacI SanDI SapI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AlfI AscI AseI
AsiSI BmtI BplI BsaAI BsiEI BsiWI BspEI BssHII
BstEII BstZ17I DraIII DrdI EagI EcoRI FseI FspI
FspAI MluI NaeI NgoMIV NheI NotI NruI PacI
PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacII
SalI SbfI SfiI SgfI SnaBI SrfI SspI SwaI
XbaI XhoI ZraI