Restriction map for
pFN21AE2313
(Linear) (Six-base) MAPSORT of: KIEE2313.seq Check: 5959 from: 1 to: 1875
KIEE2313.orf
With 182 enzymes: SgfI *
January 12, 2011 16:57 ..
AccI GT'mk_AC
Cuts at: 0 1628 1875
Size: 1628 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1875
Size: 374 1501
AflIII A'CryG_T
Cuts at: 0 912 1875
Size: 912 963
ApoI r'AATT_y
Cuts at: 0 224 497 862 1370 1875
Size: 224 273 365 508 505
Fragments arranged by size:
508 505 365 273 224
AseI AT'TA_AT
Cuts at: 0 266 628 1433 1875
Size: 266 362 805 442
Fragments arranged by size:
805 442 362 266
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 298 331 1875
Size: 298 33 1544
Fragments arranged by size:
1544 298 33
BamHI G'GATC_C
Cuts at: 0 1698 1875
Size: 1698 177
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1875
Size: 1160 715
BglII A'GATC_T
Cuts at: 0 1749 1875
Size: 1749 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 967 1875
Size: 470 497 908
Fragments arranged by size:
908 497 470
Bpu10I CC'TnA_GC
Cuts at: 0 1227 1875
Size: 1227 648
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1548 1867 1875
Size: 147 1401 319 8
Fragments arranged by size:
1401 319 147 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 1178 1875
Size: 1178 697
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 563 593 1875
Size: 563 30 1282
Fragments arranged by size:
1282 563 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 964 1875
Size: 964 911
BsiEI CG_ry'CG
Cuts at: 0 1038 1875
Size: 1038 837
BsrDI GCAATG_nn'
Cuts at: 0 1814 1875
Size: 1814 61
BsrGI T'GTAC_A
Cuts at: 0 1350 1875
Size: 1350 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 377 1875
Size: 377 1498
BstYI r'GATC_y
Cuts at: 0 708 1698 1749 1875
Size: 708 990 51 126
Fragments arranged by size:
990 708 126 51
BtgI C'CryG_G
Cuts at: 0 1448 1875
Size: 1448 427
BtsI GCAGTG_nn'
Cuts at: 0 169 1875
Size: 169 1706
EaeI y'GGCC_r
Cuts at: 0 1493 1875
Size: 1493 382
EarI CTCTTCn'nnn_
Cuts at: 0 1709 1875
Size: 1709 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 470 967 1875
Size: 374 96 497 908
Fragments arranged by size:
908 497 374 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 716 1875
Size: 716 1159
EcoO109I rG'GnC_Cy
Cuts at: 0 721 1875
Size: 721 1154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 775 807 880 912 1325 1357 1875
Size: 775 32 73 32 413 32 518
Fragments arranged by size:
775 518 413 73 32 32 32
HincII GTy'rAC
Cuts at: 0 123 1344 1875
Size: 123 1221 531
Fragments arranged by size:
1221 531 123
HindIII A'AGCT_T
Cuts at: 0 83 1875
Size: 83 1792
HpaI GTT'AAC
Cuts at: 0 123 1875
Size: 123 1752
MscI TGG'CCA
Cuts at: 0 1495 1875
Size: 1495 380
MslI CAynn'nnrTG
Cuts at: 0 1217 1875
Size: 1217 658
MspA1I CmG'CkG
Cuts at: 0 352 1875
Size: 352 1523
NcoI C'CATG_G
Cuts at: 0 1448 1875
Size: 1448 427
NspI r_CATG'y
Cuts at: 0 58 916 1875
Size: 58 858 959
Fragments arranged by size:
959 858 58
PciI A'CATG_T
Cuts at: 0 912 1875
Size: 912 963
PfoI T'CCnGG_A
Cuts at: 0 1721 1875
Size: 1721 154
PpuMI rG'GwC_Cy
Cuts at: 0 721 1875
Size: 721 1154
PstI C_TGCA'G
Cuts at: 0 263 413 1875
Size: 263 150 1462
Fragments arranged by size:
1462 263 150
PvuI CG_AT'CG
Cuts at: 0 1038 1875
Size: 1038 837
PvuII CAG'CTG
Cuts at: 0 352 1875
Size: 352 1523
ScaI AGT'ACT
Cuts at: 0 617 1875
Size: 617 1258
SfcI C'TryA_G
Cuts at: 0 259 409 1875
Size: 259 150 1466
Fragments arranged by size:
1466 259 150
SmlI C'TyrA_G
Cuts at: 0 126 1563 1846 1875
Size: 126 1437 283 29
Fragments arranged by size:
1437 283 126 29
SpeI A'CTAG_T
Cuts at: 0 10 1875
Size: 10 1865
SspI AAT'ATT
Cuts at: 0 1325 1875
Size: 1325 550
StuI AGG'CCT
Cuts at: 0 955 1875
Size: 955 920
StyI C'CwwG_G
Cuts at: 0 272 376 1448 1875
Size: 272 104 1072 427
Fragments arranged by size:
1072 427 272 104
TatI w'GTAC_w
Cuts at: 0 615 1350 1875
Size: 615 735 525
Fragments arranged by size:
735 615 525
XbaI T'CTAG_A
Cuts at: 0 705 1620 1875
Size: 705 915 255
Fragments arranged by size:
915 705 255
XmnI GAAnn'nnTTC
Cuts at: 0 683 1875
Size: 683 1192
Enzymes that do cut:
AccI AcuI AflIII ApoI AseI BaeI BamHI BbsI
BglII BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsiEI
BsrDI BsrGI BstXI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII HpaI MscI
MslI MspA1I NcoI NspI PciI PfoI PpuMI PstI
PvuI PvuII ScaI SfcI SmlI SpeI SspI StuI
StyI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI