Restriction map for
pFN21AE2306
(Linear) (Six-base) MAPSORT of: KIEE2306.seq Check: 6768 from: 1 to: 891
KIEE2306.orf
With 182 enzymes: SgfI *
November 16, 2010 13:28 ..
Acc65I G'GTAC_C
Cuts at: 0 620 891
Size: 620 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 891
Size: 358 533
AhdI GACnn_n'nnGTC
Cuts at: 0 710 891
Size: 710 181
AlwNI CAG_nnn'CTG
Cuts at: 0 204 273 477 891
Size: 204 69 204 414
Fragments arranged by size:
414 204 204 69
ApaI G_GGCC'C
Cuts at: 0 835 891
Size: 835 56
ApoI r'AATT_y
Cuts at: 0 61 364 891
Size: 61 303 527
Fragments arranged by size:
527 303 61
AvaI C'yCGr_G
Cuts at: 0 280 604 698 891
Size: 280 324 94 193
Fragments arranged by size:
324 280 193 94
BanI G'GyrC_C
Cuts at: 0 27 620 891
Size: 27 593 271
Fragments arranged by size:
593 271 27
BanII G_rGCy'C
Cuts at: 0 245 313 604 835 875 891
Size: 245 68 291 231 40 16
Fragments arranged by size:
291 245 231 68 40 16
BbvCI CC'TCA_GC
Cuts at: 0 421 891
Size: 421 470
BciVI GTATCCnnnnn_n'
Cuts at: 0 577 891
Size: 577 314
BglI GCCn_nnn'nGGC
Cuts at: 0 42 163 891
Size: 42 121 728
Fragments arranged by size:
728 121 42
BglII A'GATC_T
Cuts at: 0 807 891
Size: 807 84
Bme1580I G_kGCm'C
Cuts at: 0 32 229 359 414 835 891
Size: 32 197 130 55 421 56
Fragments arranged by size:
421 197 130 56 55 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 618 646 891
Size: 119 499 28 245
Fragments arranged by size:
499 245 119 28
Bpu10I CC'TnA_GC
Cuts at: 0 421 891
Size: 421 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 891
Size: 529 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 622 891
Size: 622 269
BsaAI yAC'GTr
Cuts at: 0 224 891
Size: 224 667
BsaBI GATnn'nnATC
Cuts at: 0 565 891
Size: 565 326
BsaHI Gr'CG_yC
Cuts at: 0 259 891
Size: 259 632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 891
Size: 679 30 182
Fragments arranged by size:
679 182 30
BseYI C'CCAG_C
Cuts at: 0 32 214 393 533 613 891
Size: 32 182 179 140 80 278
Fragments arranged by size:
278 182 179 140 80 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 564 850 891
Size: 427 137 286 41
Fragments arranged by size:
427 286 137 41
BsiHKAI G_wGCw'C
Cuts at: 0 406 891
Size: 406 485
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 699 891
Size: 250 449 192
Fragments arranged by size:
449 250 192
Bsp1286I G_dGCh'C
Cuts at: 0 32 229 245 313 359 406 414 604
Size: 32 197 16 68 46 47 8 190
Cuts at: 604 835 875 891
Size: 231 40 16
Fragments arranged by size:
231 197 190 68 47 46 40 32
16 16 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 891
Size: 449 442
BssSI C'ACGA_G
Cuts at: 0 868 891
Size: 868 23
BstYI r'GATC_y
Cuts at: 0 528 772 807 891
Size: 528 244 35 84
Fragments arranged by size:
528 244 84 35
Bsu36I CC'TnA_GG
Cuts at: 0 39 891
Size: 39 852
BtgI C'CryG_G
Cuts at: 0 459 891
Size: 459 432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 774 891
Size: 774 117
EaeI y'GGCC_r
Cuts at: 0 104 572 891
Size: 104 468 319
Fragments arranged by size:
468 319 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 358 618 646 891
Size: 119 239 260 28 245
Fragments arranged by size:
260 245 239 119 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 206 891
Size: 79 127 685
Fragments arranged by size:
685 127 79
EcoO109I rG'GnC_Cy
Cuts at: 0 90 270 501 540 832 891
Size: 90 180 231 39 292 59
Fragments arranged by size:
292 231 180 90 59 39
FspI TGC'GCA
Cuts at: 0 349 891
Size: 349 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 312 344 679 696 711 728 891
Size: 312 32 335 17 15 17 163
Fragments arranged by size:
335 312 163 32 17 17 15
HincII GTy'rAC
Cuts at: 0 735 891
Size: 735 156
KpnI G_GTAC'C
Cuts at: 0 624 891
Size: 624 267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 891
Size: 317 574
MscI TGG'CCA
Cuts at: 0 106 891
Size: 106 785
MslI CAynn'nnrTG
Cuts at: 0 304 891
Size: 304 587
MspA1I CmG'CkG
Cuts at: 0 658 891
Size: 658 233
NcoI C'CATG_G
Cuts at: 0 459 891
Size: 459 432
PasI CC'CwG_GG
Cuts at: 0 7 359 891
Size: 7 352 532
Fragments arranged by size:
532 352 7
PfoI T'CCnGG_A
Cuts at: 0 96 891
Size: 96 795
PmlI CAC'GTG
Cuts at: 0 224 891
Size: 224 667
PpuMI rG'GwC_Cy
Cuts at: 0 90 501 891
Size: 90 411 390
Fragments arranged by size:
411 390 90
PspOMI G'GGCC_C
Cuts at: 0 831 891
Size: 831 60
PstI C_TGCA'G
Cuts at: 0 473 891
Size: 473 418
SfcI C'TryA_G
Cuts at: 0 469 891
Size: 469 422
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 163 891
Size: 163 728
SmaI CCC'GGG
Cuts at: 0 282 700 891
Size: 282 418 191
Fragments arranged by size:
418 282 191
SmlI C'TyrA_G
Cuts at: 0 544 891
Size: 544 347
StuI AGG'CCT
Cuts at: 0 640 827 891
Size: 640 187 64
Fragments arranged by size:
640 187 64
StyI C'CwwG_G
Cuts at: 0 459 891
Size: 459 432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 461 891
Size: 461 430
Tth111I GACn'n_nGTC
Cuts at: 0 691 891
Size: 691 200
XmaI C'CCGG_G
Cuts at: 0 280 698 891
Size: 280 418 193
Fragments arranged by size:
418 280 193
XmnI GAAnn'nnTTC
Cuts at: 0 487 891
Size: 487 404
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbvCI BciVI BglI BglII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BssSI BstYI Bsu36I BtgI
BtgZI EaeI Eco57MI EcoNI EcoO109I FspI Hin4I HincII
KpnI MmeI MscI MslI MspA1I NcoI PasI PfoI
PmlI PpuMI PspOMI PstI SfcI SfiI SmaI SmlI
StuI StyI TaqII Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BclI BfrBI BlpI
BmgBI BmrI BmtI BplI BsaWI BseRI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XbaI XcmI XhoI ZraI