Restriction map for
pFN21AE2303
(Linear) (Six-base) MAPSORT of: KIEE2303.seq Check: 7459 from: 1 to: 6630
KIEE2303.orf
With 182 enzymes: SgfI *
November 26, 2010 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 618 2487 6630
Size: 618 1869 4143
Fragments arranged by size:
4143 1869 618
AccI GT'mk_AC
Cuts at: 0 1777 4331 6630
Size: 1777 2554 2299
Fragments arranged by size:
2554 2299 1777
Acc65I G'GTAC_C
Cuts at: 0 2254 6630
Size: 2254 4376
AclI AA'CG_TT
Cuts at: 0 1296 6630
Size: 1296 5334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 2878 2911 4296 4796 6630
Size: 827 2051 33 1385 500 1834
Fragments arranged by size:
2051 1834 1385 827 500 33
AfeI AGC'GCT
Cuts at: 0 3078 5509 6630
Size: 3078 2431 1121
Fragments arranged by size:
3078 2431 1121
AgeI A'CCGG_T
Cuts at: 0 4755 6630
Size: 4755 1875
AleI CACnn'nnGTG
Cuts at: 0 538 2290 3061 6630
Size: 538 1752 771 3569
Fragments arranged by size:
3569 1752 771 538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1049 2020 2375 6630
Size: 1049 971 355 4255
Fragments arranged by size:
4255 1049 971 355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 986 1018 6127 6159 6630
Size: 986 32 5109 32 471
Fragments arranged by size:
5109 986 471 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 4037 4422 5893 6282 6630
Size: 4037 385 1471 389 348
Fragments arranged by size:
4037 1471 389 385 348
ApaI G_GGCC'C
Cuts at: 0 1313 4640 6630
Size: 1313 3327 1990
Fragments arranged by size:
3327 1990 1313
ApaLI G'TGCA_C
Cuts at: 0 2803 3910 6630
Size: 2803 1107 2720
Fragments arranged by size:
2803 2720 1107
ApoI r'AATT_y
Cuts at: 0 2734 3567 3720 4979 5157 6444 6630
Size: 2734 833 153 1259 178 1287 186
Fragments arranged by size:
2734 1287 1259 833 186 178 153
AseI AT'TA_AT
Cuts at: 0 6124 6630
Size: 6124 506
AvaI C'yCGr_G
Cuts at: 0 2776 6630
Size: 2776 3854
AvrII C'CTAG_G
Cuts at: 0 1005 6630
Size: 1005 5625
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3462 3495 6630
Size: 3462 33 3135
Fragments arranged by size:
3462 3135 33
BamHI G'GATC_C
Cuts at: 0 1245 2836 6630
Size: 1245 1591 3794
Fragments arranged by size:
3794 1591 1245
BanI G'GyrC_C
Cuts at: 0 395 2254 2292 2409 6630
Size: 395 1859 38 117 4221
Fragments arranged by size:
4221 1859 395 117 38
BanII G_rGCy'C
Cuts at: 0 1313 1952 2701 3290 4640 4924 5864 5908
Size: 1313 639 749 589 1350 284 940 44
Cuts at: 5908 6205 6630
Size: 297 425
Fragments arranged by size:
1350 1313 940 749 639 589 425 297
284 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 773 2525 6232 6630
Size: 596 177 1752 3707 398
Fragments arranged by size:
3707 1752 596 398 177
BbvCI CC'TCA_GC
Cuts at: 0 1682 6630
Size: 1682 4948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1888 1922 6630
Size: 1888 34 4708
Fragments arranged by size:
4708 1888 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5749 6630
Size: 5749 881
BclI T'GATC_A
Cuts at: 0 572 6630
Size: 572 6058
BfrBI ATG'CAT
Cuts at: 0 1151 1493 6630
Size: 1151 342 5137
Fragments arranged by size:
5137 1151 342
Bme1580I G_kGCm'C
Cuts at: 0 1313 2807 3193 3914 4640 6373 6630
Size: 1313 1494 386 721 726 1733 257
Fragments arranged by size:
1733 1494 1313 726 721 386 257
BmgBI CAC'GTC
Cuts at: 0 6485 6630
Size: 6485 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 1161 1913 2058 4862 6140 6157 6324
Size: 27 1134 752 145 2804 1278 17 167
Cuts at: 6324 6630
Size: 306
Fragments arranged by size:
2804 1278 1134 752 306 167 145 27
17
BmtI G_CTAG'C
Cuts at: 0 1220 6630
Size: 1220 5410
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2004 4943 5013 5091 6630
Size: 2004 2939 70 78 1539
Fragments arranged by size:
2939 2004 1539 78 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 923 974 1750 1928 1936 2135 2364
Size: 61 862 51 776 178 8 199 229
Cuts at: 2364 3104 3670 3795 5006 5155 5700 6008 6630
Size: 740 566 125 1211 149 545 308 622
Fragments arranged by size:
1211 862 776 740 622 566 545 308
229 199 178 149 125 61 51 8
Bpu10I CC'TnA_GC
Cuts at: 0 1682 4130 4641 5144 5360 6630
Size: 1682 2448 511 503 216 1270
Fragments arranged by size:
2448 1682 1270 511 503 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4341 5335 5767 5948 6630
Size: 4341 994 432 181 682
Fragments arranged by size:
4341 994 682 432 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 1437 4764 5548 6630
Size: 1437 3327 784 1082
Fragments arranged by size:
3327 1437 1082 784
BsaAI yAC'GTr
Cuts at: 0 4381 5057 5614 6539 6630
Size: 4381 676 557 925 91
Fragments arranged by size:
4381 925 676 557 91
BsaBI GATnn'nnATC
Cuts at: 0 3361 6630
Size: 3361 3269
BsaHI Gr'CG_yC
Cuts at: 0 216 5706 6630
Size: 216 5490 924
Fragments arranged by size:
5490 924 216
BsaWI w'CCGG_w
Cuts at: 0 4755 6630
Size: 4755 1875
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1748 1778 6216 6246 6630
Size: 1748 30 4438 30 384
Fragments arranged by size:
4438 1748 384 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 1191 1533 3667 3670 3673 3945 4486
Size: 595 596 342 2134 3 3 272 541
Cuts at: 4486 5083 5610 6277 6630
Size: 597 527 667 353
Fragments arranged by size:
2134 667 597 596 595 541 527 353
342 272 3 3
BseYI C'CCAG_C
Cuts at: 0 147 551 687 3084 4633 4668 4846 5368
Size: 147 404 136 2397 1549 35 178 522
Cuts at: 5368 5433 6630
Size: 65 1197
Fragments arranged by size:
2397 1549 1197 522 404 178 147 136
65 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1322 1370 4078 5292 5511 5594 6630
Size: 596 726 48 2708 1214 219 83 1036
Fragments arranged by size:
2708 1214 1036 726 596 219 83 48
BsiHKAI G_wGCw'C
Cuts at: 0 1952 1999 2807 3914 4924 5477 6596 6630
Size: 1952 47 808 1107 1010 553 1119 34
Fragments arranged by size:
1952 1119 1107 1010 808 553 47 34
BsmI GAATG_Cn'
Cuts at: 0 1153 3826 4635 6630
Size: 1153 2673 809 1995
Fragments arranged by size:
2673 1995 1153 809
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1915 5434 6630
Size: 1915 3519 1196
Fragments arranged by size:
3519 1915 1196
Bsp1286I G_dGCh'C
Cuts at: 0 1313 1952 1999 2701 2807 3193 3290 3914
Size: 1313 639 47 702 106 386 97 624
Cuts at: 3914 4640 4924 5477 5864 5908 6205 6373 6596
Size: 726 284 553 387 44 297 168 223
Cuts at: 6596 6630
Size: 34
Fragments arranged by size:
1313 726 702 639 624 553 387 386
297 284 223 168 106 97 47 44
34
BspHI T'CATG_A
Cuts at: 0 297 6617 6630
Size: 297 6320 13
Fragments arranged by size:
6320 297 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 297 618 2487 5566 6630
Size: 209 88 321 1869 3079 1064
Fragments arranged by size:
3079 1869 1064 321 209 88
BsrBI CCG'CTC
Cuts at: 0 4466 5364 6630
Size: 4466 898 1266
Fragments arranged by size:
4466 1266 898
BsrDI GCAATG_nn'
Cuts at: 0 911 2002 2092 3936 6630
Size: 911 1091 90 1844 2694
Fragments arranged by size:
2694 1844 1091 911 90
BsrFI r'CCGG_y
Cuts at: 0 2289 4755 6630
Size: 2289 2466 1875
Fragments arranged by size:
2466 2289 1875
BsrGI T'GTAC_A
Cuts at: 0 5977 6605 6630
Size: 5977 628 25
Fragments arranged by size:
5977 628 25
BssSI C'ACGA_G
Cuts at: 0 1688 6630
Size: 1688 4942
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 1044 4073 4155 6018 6630
Size: 282 762 3029 82 1863 612
Fragments arranged by size:
3029 1863 762 612 282 82
BstYI r'GATC_y
Cuts at: 0 1245 2836 3024 5294 6630
Size: 1245 1591 188 2270 1336
Fragments arranged by size:
2270 1591 1336 1245 188
BstZ17I GTA'TAC
Cuts at: 0 4332 6630
Size: 4332 2298
Bsu36I CC'TnA_GG
Cuts at: 0 3629 6630
Size: 3629 3001
BtgI C'CryG_G
Cuts at: 0 4985 5246 5538 5715 5855 6630
Size: 4985 261 292 177 140 775
Fragments arranged by size:
4985 775 292 261 177 140
BtsI GCAGTG_nn'
Cuts at: 0 1997 3191 4683 5281 6630
Size: 1997 1194 1492 598 1349
Fragments arranged by size:
1997 1492 1349 1194 598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2591 2626 6630
Size: 2591 35 4004
Fragments arranged by size:
4004 2591 35
DraI TTT'AAA
Cuts at: 0 1292 2040 3369 6630
Size: 1292 748 1329 3261
Fragments arranged by size:
3261 1329 1292 748
DraIII CAC_nnn'GTG
Cuts at: 0 1921 6588 6630
Size: 1921 4667 42
Fragments arranged by size:
4667 1921 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 1795 5705 6630
Size: 1795 3910 925
Fragments arranged by size:
3910 1795 925
EaeI y'GGCC_r
Cuts at: 0 1478 1586 5712 5916 6548 6630
Size: 1478 108 4126 204 632 82
Fragments arranged by size:
4126 1478 632 204 108 82
EarI CTCTTCn'nnn_
Cuts at: 0 370 1202 2538 3538 3764 4812 4929 5482
Size: 370 832 1336 1000 226 1048 117 553
Cuts at: 5482 6162 6382 6630
Size: 680 220 248
Fragments arranged by size:
1336 1048 1000 832 680 553 370 248
226 220 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3442 6630
Size: 3442 3188
EcoICRI GAG'CTC
Cuts at: 0 1950 4922 6630
Size: 1950 2972 1708
Fragments arranged by size:
2972 1950 1708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 827 923 974 1750 1928 1936 2135
Size: 61 766 96 51 776 178 8 199
Cuts at: 2135 2364 2878 2911 3104 3670 3795 4296 4796
Size: 229 514 33 193 566 125 501 500
Cuts at: 4796 5006 5155 5700 6008 6630
Size: 210 149 545 308 622
Fragments arranged by size:
776 766 622 566 545 514 501 500
308 229 210 199 193 178 149 125
96 61 51 33 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2392 3628 6630
Size: 2392 1236 3002
Fragments arranged by size:
3002 2392 1236
EcoO109I rG'GnC_Cy
Cuts at: 0 948 1469 1865 3164 4034 4637 5870 6630
Size: 948 521 396 1299 870 603 1233 760
Fragments arranged by size:
1299 1233 948 870 760 603 521 396
EcoRI G'AATT_C
Cuts at: 0 2734 3567 4979 6444 6630
Size: 2734 833 1412 1465 186
Fragments arranged by size:
2734 1465 1412 833 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1464 6630
Size: 1464 5166
FspI TGC'GCA
Cuts at: 0 4508 6630
Size: 4508 2122
HaeII r_GCGC'y
Cuts at: 0 3080 3600 5511 6630
Size: 3080 520 1911 1119
Fragments arranged by size:
3080 1911 1119 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 366 398 472 504 1065 1097
Size: 37 32 297 32 74 32 561 32
Cuts at: 1097 1705 1737 1748 1780 1805 1837 3223 3255
Size: 608 32 11 32 25 32 1386 32
Cuts at: 3255 5675 5707 6630
Size: 2420 32 923
Fragments arranged by size:
2420 1386 923 608 561 297 74 37
32 32 32 32 32 32 32 32
32 25 11
HincII GTy'rAC
Cuts at: 0 471 1190 6081 6630
Size: 471 719 4891 549
Fragments arranged by size:
4891 719 549 471
HindIII A'AGCT_T
Cuts at: 0 1285 6630
Size: 1285 5345
KpnI G_GTAC'C
Cuts at: 0 2258 6630
Size: 2258 4372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 465 1219 1273 3530 3815 4205 4812
Size: 80 385 754 54 2257 285 390 607
Cuts at: 4812 6202 6467 6630
Size: 1390 265 163
Fragments arranged by size:
2257 1390 754 607 390 385 285 265
163 80 54
MscI TGG'CCA
Cuts at: 0 5714 6630
Size: 5714 916
MslI CAynn'nnrTG
Cuts at: 0 538 2290 3061 3929 3946 4247 4396 5038
Size: 538 1752 771 868 17 301 149 642
Cuts at: 5038 5125 5341 5989 6016 6124 6630
Size: 87 216 648 27 108 506
Fragments arranged by size:
1752 868 771 648 642 538 506 301
216 149 108 87 27 17
MspA1I CmG'CkG
Cuts at: 0 185 1265 3389 3476 4672 5026 6453 6630
Size: 185 1080 2124 87 1196 354 1427 177
Fragments arranged by size:
2124 1427 1196 1080 354 185 177 87
NcoI C'CATG_G
Cuts at: 0 5246 5538 5715 5855 6630
Size: 5246 292 177 140 775
Fragments arranged by size:
5246 775 292 177 140
NdeI CA'TA_TG
Cuts at: 0 1124 2799 4233 5150 6630
Size: 1124 1675 1434 917 1480
Fragments arranged by size:
1675 1480 1434 1124 917
NheI G'CTAG_C
Cuts at: 0 1216 6630
Size: 1216 5414
NsiI A_TGCA'T
Cuts at: 0 1153 1495 6630
Size: 1153 342 5135
Fragments arranged by size:
5135 1153 342
NspI r_CATG'y
Cuts at: 0 3951 4057 5196 5940 6630
Size: 3951 106 1139 744 690
Fragments arranged by size:
3951 1139 744 690 106
PasI CC'CwG_GG
Cuts at: 0 4912 5865 6630
Size: 4912 953 765
Fragments arranged by size:
4912 953 765
PflMI CCAn_nnn'nTGG
Cuts at: 0 3966 4092 6588 6630
Size: 3966 126 2496 42
Fragments arranged by size:
3966 2496 126 42
PfoI T'CCnGG_A
Cuts at: 0 3725 6630
Size: 3725 2905
PmlI CAC'GTG
Cuts at: 0 6539 6630
Size: 6539 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2537 2569 6630
Size: 2537 32 4061
Fragments arranged by size:
4061 2537 32
PpuMI rG'GwC_Cy
Cuts at: 0 948 3164 4034 5870 6630
Size: 948 2216 870 1836 760
Fragments arranged by size:
2216 1836 948 870 760
PsiI TTA'TAA
Cuts at: 0 2648 6630
Size: 2648 3982
PspOMI G'GGCC_C
Cuts at: 0 1309 4636 6630
Size: 1309 3327 1994
Fragments arranged by size:
3327 1994 1309
PstI C_TGCA'G
Cuts at: 0 308 2508 2970 3591 3992 4553 4722 5004
Size: 308 2200 462 621 401 561 169 282
Cuts at: 5004 5067 5201 5331 6630
Size: 63 134 130 1299
Fragments arranged by size:
2200 1299 621 561 462 401 308 282
169 134 130 63
PvuII CAG'CTG
Cuts at: 0 1265 3389 4672 5026 6630
Size: 1265 2124 1283 354 1604
Fragments arranged by size:
2124 1604 1283 1265 354
SacI G_AGCT'C
Cuts at: 0 1952 4924 6630
Size: 1952 2972 1706
Fragments arranged by size:
2972 1952 1706
SapI GCTCTTCn'nnn_
Cuts at: 0 370 1202 2538 4929 5482 6630
Size: 370 832 1336 2391 553 1148
Fragments arranged by size:
2391 1336 1148 832 553 370
ScaI AGT'ACT
Cuts at: 0 626 6630
Size: 626 6004
SexAI A'CCwGG_T
Cuts at: 0 1043 2274 6630
Size: 1043 1231 4356
Fragments arranged by size:
4356 1231 1043
SfcI C'TryA_G
Cuts at: 0 304 2504 2966 3587 3988 4549 4718 5000
Size: 304 2200 462 621 401 561 169 282
Cuts at: 5000 5063 5197 5327 5626 5761 6630
Size: 63 134 130 299 135 869
Fragments arranged by size:
2200 869 621 561 462 401 304 299
282 169 135 134 130 63
SgrAI Cr'CCGG_yG
Cuts at: 0 2289 6630
Size: 2289 4341
SmlI C'TyrA_G
Cuts at: 0 4320 5350 5782 5963 6630
Size: 4320 1030 432 181 667
Fragments arranged by size:
4320 1030 667 432 181
SphI G_CATG'C
Cuts at: 0 3951 6630
Size: 3951 2679
SspI AAT'ATT
Cuts at: 0 5571 6630
Size: 5571 1059
StuI AGG'CCT
Cuts at: 0 1941 4285 6630
Size: 1941 2344 2345
Fragments arranged by size:
2345 2344 1941
StyI C'CwwG_G
Cuts at: 0 1005 2258 4459 5246 5538 5715 5745 5855
Size: 1005 1253 2201 787 292 177 30 110
Cuts at: 5855 6288 6630
Size: 433 342
Fragments arranged by size:
2201 1253 1005 787 433 342 292 177
110 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 9 274 1713 1932 2537 3678 6568 6630
Size: 9 265 1439 219 605 1141 2890 62
Fragments arranged by size:
2890 1439 1141 605 265 219 62 9
TatI w'GTAC_w
Cuts at: 0 624 5189 5261 5977 6515 6605 6630
Size: 624 4565 72 716 538 90 25
Fragments arranged by size:
4565 716 624 538 90 72 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1709 1741 2066 2098 3609 3641
Size: 1033 32 644 32 325 32 1511 32
Cuts at: 3641 3707 3739 4394 4426 5290 5322 6182 6214
Size: 66 32 655 32 864 32 860 32
Cuts at: 6214 6216 6248 6528 6560 6630
Size: 2 32 280 32 70
Fragments arranged by size:
1511 1033 864 860 655 644 325 280
70 66 32 32 32 32 32 32
32 32 32 32 2
XbaI T'CTAG_A
Cuts at: 0 6247 6630
Size: 6247 383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 916 6630
Size: 916 5714
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI
BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI
SgrAI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AatII AflII AflIII AhdI AscI AsiSI BbeI BglI
BglII BlpI BsiEI BsiWI BspEI BssHII BstAPI BstBI
BstEII BtgZI ClaI EagI EcoRV FseI FspAI HpaI
KasI MfeI MluI NaeI NarI NgoMIV NotI NruI
PacI PciI PmeI PshAI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SfiI SfoI SgfI SmaI
SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI XmnI
ZraI