Restriction map for
pFN21AE2302
(Linear) (Six-base) MAPSORT of: KIEE2302.seq Check: 2032 from: 1 to: 6522
KIEE2302.orf
With 182 enzymes: SgfI *
November 26, 2010 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4917 6522
Size: 4917 1605
AccI GT'mk_AC
Cuts at: 0 557 4717 5517 6522
Size: 557 4160 800 1005
Fragments arranged by size:
4160 1005 800 557
AclI AA'CG_TT
Cuts at: 0 3483 6522
Size: 3483 3039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1031 2840 3218 3360 3838 3850 4535
Size: 770 261 1809 378 142 478 12 685
Cuts at: 4535 4556 5900 6160 6299 6362 6522
Size: 21 1344 260 139 63 160
Fragments arranged by size:
1809 1344 770 685 478 378 261 260
160 142 139 63 21 12
AflII C'TTAA_G
Cuts at: 0 181 6286 6522
Size: 181 6105 236
Fragments arranged by size:
6105 236 181
AflIII A'CryG_T
Cuts at: 0 3516 3663 3734 5681 6522
Size: 3516 147 71 1947 841
Fragments arranged by size:
3516 1947 841 147 71
AhdI GACnn_n'nnGTC
Cuts at: 0 1743 6522
Size: 1743 4779
AleI CACnn'nnGTG
Cuts at: 0 1088 1433 1619 6522
Size: 1088 345 186 4903
Fragments arranged by size:
4903 1088 345 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4380 6522
Size: 4380 2142
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2585 2617 5481 5513 6522
Size: 2585 32 2864 32 1009
Fragments arranged by size:
2864 2585 1009 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1054 5082 5785 6522
Size: 1054 4028 703 737
Fragments arranged by size:
4028 1054 737 703
ApaLI G'TGCA_C
Cuts at: 0 2969 3585 6522
Size: 2969 616 2937
Fragments arranged by size:
2969 2937 616
ApoI r'AATT_y
Cuts at: 0 402 1039 1584 1909 2116 2726 2996 3111
Size: 402 637 545 325 207 610 270 115
Cuts at: 3111 3246 3397 4431 5509 5937 6522
Size: 135 151 1034 1078 428 585
Fragments arranged by size:
1078 1034 637 610 585 545 428 402
325 270 207 151 135 115
AseI AT'TA_AT
Cuts at: 0 694 6035 6522
Size: 694 5341 487
Fragments arranged by size:
5341 694 487
AvrII C'CTAG_G
Cuts at: 0 4377 6522
Size: 4377 2145
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1793 1826 6522
Size: 1793 33 4696
Fragments arranged by size:
4696 1793 33
BamHI G'GATC_C
Cuts at: 0 889 4708 6522
Size: 889 3819 1814
Fragments arranged by size:
3819 1814 889
BanI G'GyrC_C
Cuts at: 0 1326 1570 3310 6522
Size: 1326 244 1740 3212
Fragments arranged by size:
3212 1740 1326 244
BanII G_rGCy'C
Cuts at: 0 5484 6522
Size: 5484 1038
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 266 953 6522
Size: 155 111 687 5569
Fragments arranged by size:
5569 687 155 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 2510 2574 4913 5371 5853 6522
Size: 2510 64 2339 458 482 669
Fragments arranged by size:
2510 2339 669 482 458 64
BclI T'GATC_A
Cuts at: 0 5394 6522
Size: 5394 1128
BfrBI ATG'CAT
Cuts at: 0 1566 2095 6522
Size: 1566 529 4427
Fragments arranged by size:
4427 1566 529
BglII A'GATC_T
Cuts at: 0 2484 4170 5424 6522
Size: 2484 1686 1254 1098
Fragments arranged by size:
2484 1686 1254 1098
Bme1580I G_kGCm'C
Cuts at: 0 2973 3589 6522
Size: 2973 616 2933
Fragments arranged by size:
2973 2933 616
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 1743 5154 6522
Size: 260 1483 3411 1368
Fragments arranged by size:
3411 1483 1368 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 2612 4814 5285 6062 6522
Size: 1487 1125 2202 471 777 460
Fragments arranged by size:
2202 1487 1125 777 471 460
Bpu10I CC'TnA_GC
Cuts at: 0 1361 4515 5541 6522
Size: 1361 3154 1026 981
Fragments arranged by size:
3154 1361 1026 981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 3074 4330 6242 6522
Size: 1279 1795 1256 1912 280
Fragments arranged by size:
1912 1795 1279 1256 280
BsaAI yAC'GTr
Cuts at: 0 412 3664 4901 6309 6522
Size: 412 3252 1237 1408 213
Fragments arranged by size:
3252 1408 1237 412 213
BsaHI Gr'CG_yC
Cuts at: 0 3717 6522
Size: 3717 2805
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2585 2615 3734 3764 5016 5046 6048 6078
Size: 2585 30 1119 30 1252 30 1002 30
Cuts at: 6078 6522
Size: 444
Fragments arranged by size:
2585 1252 1119 1002 444 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1547 1652 3041 3050 3835 5024 6522
Size: 1547 105 1389 9 785 1189 1498
Fragments arranged by size:
1547 1498 1389 1189 785 105 9
BseYI C'CCAG_C
Cuts at: 0 834 4424 4783 5123 6522
Size: 834 3590 359 340 1399
Fragments arranged by size:
3590 1399 834 359 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2035 6522
Size: 2035 4487
BsiHKAI G_wGCw'C
Cuts at: 0 1164 2973 3090 3589 3823 4966 6522
Size: 1164 1809 117 499 234 1143 1556
Fragments arranged by size:
1809 1556 1164 1143 499 234 117
BsmI GAATG_Cn'
Cuts at: 0 346 661 1150 3845 5723 6522
Size: 346 315 489 2695 1878 799
Fragments arranged by size:
2695 1878 799 489 346 315
BsmBI CGTCTCn'nnnn_
Cuts at: 0 992 6522
Size: 992 5530
Bsp1286I G_dGCh'C
Cuts at: 0 1164 2973 3090 3589 3823 4966 5484 6522
Size: 1164 1809 117 499 234 1143 518 1038
Fragments arranged by size:
1809 1164 1143 1038 518 499 234 117
BspHI T'CATG_A
Cuts at: 0 3386 4244 6153 6522
Size: 3386 858 1909 369
Fragments arranged by size:
3386 1909 858 369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3087 3183 4917 6211 6522
Size: 3087 96 1734 1294 311
Fragments arranged by size:
3087 1734 1294 311 96
BsrDI GCAATG_nn'
Cuts at: 0 522 1984 3693 5563 5753 6522
Size: 522 1462 1709 1870 190 769
Fragments arranged by size:
1870 1709 1462 769 522 190
BsrGI T'GTAC_A
Cuts at: 0 5058 5550 6069 6522
Size: 5058 492 519 453
Fragments arranged by size:
5058 519 492 453
BssSI C'ACGA_G
Cuts at: 0 3062 6522
Size: 3062 3460
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2062 3692 6522
Size: 2062 1630 2830
Fragments arranged by size:
2830 2062 1630
BstBI TT'CG_AA
Cuts at: 0 3837 6522
Size: 3837 2685
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1090 6522
Size: 1090 5432
BstYI r'GATC_y
Cuts at: 0 889 948 1698 2389 2484 3336 3624 4090
Size: 889 59 750 691 95 852 288 466
Cuts at: 4090 4170 4255 4708 5424 6082 6522
Size: 80 85 453 716 658 440
Fragments arranged by size:
889 852 750 716 691 658 466 453
440 288 95 85 80 59
BstZ17I GTA'TAC
Cuts at: 0 558 6522
Size: 558 5964
Bsu36I CC'TnA_GG
Cuts at: 0 992 6388 6522
Size: 992 5396 134
Fragments arranged by size:
5396 992 134
BtgI C'CryG_G
Cuts at: 0 1086 5228 5569 6096 6522
Size: 1086 4142 341 527 426
Fragments arranged by size:
4142 1086 527 426 341
BtsI GCAGTG_nn'
Cuts at: 0 2477 2918 2973 3823 3988 6522
Size: 2477 441 55 850 165 2534
Fragments arranged by size:
2534 2477 850 441 165 55
ClaI AT'CG_AT
Cuts at: 0 5465 6522
Size: 5465 1057
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1103 1138 2498 2533 6522
Size: 1103 35 1360 35 3989
Fragments arranged by size:
3989 1360 1103 35 35
DraI TTT'AAA
Cuts at: 0 1994 3255 5132 6522
Size: 1994 1261 1877 1390
Fragments arranged by size:
1994 1877 1390 1261
DraIII CAC_nnn'GTG
Cuts at: 0 930 6522
Size: 930 5592
DrdI GACnn_nn'nnGTC
Cuts at: 0 2329 6461 6522
Size: 2329 4132 61
Fragments arranged by size:
4132 2329 61
EaeI y'GGCC_r
Cuts at: 0 3951 6522
Size: 3951 2571
EarI CTCTTCn'nnn_
Cuts at: 0 61 225 566 2362 2798 3665 3857 4058
Size: 61 164 341 1796 436 867 192 201
Cuts at: 4058 4977 5033 6522
Size: 919 56 1489
Fragments arranged by size:
1796 1489 919 867 436 341 201 192
164 61 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1031 1487 2612 2840 3218 3360 3838
Size: 770 261 456 1125 228 378 142 478
Cuts at: 3838 3850 4535 4556 4814 5285 5900 6062 6160
Size: 12 685 21 258 471 615 162 98
Cuts at: 6160 6299 6362 6522
Size: 139 63 160
Fragments arranged by size:
1125 770 685 615 478 471 456 378
261 258 228 162 160 142 139 98
63 21 12
EcoO109I rG'GnC_Cy
Cuts at: 0 2860 3916 4381 6522
Size: 2860 1056 465 2141
Fragments arranged by size:
2860 2141 1056 465
EcoRI G'AATT_C
Cuts at: 0 2116 2996 6522
Size: 2116 880 3526
Fragments arranged by size:
3526 2116 880
EcoRV GAT'ATC
Cuts at: 0 3441 6030 6522
Size: 3441 2589 492
Fragments arranged by size:
3441 2589 492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4472 6522
Size: 4472 2050
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1003 1035 1478 1485 1510 1517 1729 1761
Size: 1003 32 443 7 25 7 212 32
Cuts at: 1761 2179 2211 2218 2250 2436 2449 2468 2481
Size: 418 32 7 32 186 13 19 13
Cuts at: 2481 3721 3753 4755 4787 5016 5048 5773 5805
Size: 1240 32 1002 32 229 32 725 32
Cuts at: 5805 6114 6146 6442 6474 6522
Size: 309 32 296 32 48
Fragments arranged by size:
1240 1003 1002 725 443 418 309 296
229 212 186 48 32 32 32 32
32 32 32 32 32 32 25 19
13 13 7 7 7
HincII GTy'rAC
Cuts at: 0 1672 4207 4232 5792 6522
Size: 1672 2535 25 1560 730
Fragments arranged by size:
2535 1672 1560 730 25
HindIII A'AGCT_T
Cuts at: 0 4158 4348 6522
Size: 4158 190 2174
Fragments arranged by size:
4158 2174 190
HpaI GTT'AAC
Cuts at: 0 4232 6522
Size: 4232 2290
MfeI C'AATT_G
Cuts at: 0 3654 4913 6522
Size: 3654 1259 1609
Fragments arranged by size:
3654 1609 1259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1908 2806 2814 3052 3300 3364 4233 4655
Size: 1908 898 8 238 248 64 869 422
Cuts at: 4655 5146 5203 5467 6079 6522
Size: 491 57 264 612 443
Fragments arranged by size:
1908 898 869 612 491 443 422 264
248 238 64 57 8
MslI CAynn'nnrTG
Cuts at: 0 1088 1433 1576 1619 3147 3521 4829 5568
Size: 1088 345 143 43 1528 374 1308 739
Cuts at: 5568 6101 6522
Size: 533 421
Fragments arranged by size:
1528 1308 1088 739 533 421 374 345
143 43
MspA1I CmG'CkG
Cuts at: 0 1219 2429 2987 4315 4783 6522
Size: 1219 1210 558 1328 468 1739
Fragments arranged by size:
1739 1328 1219 1210 558 468
NcoI C'CATG_G
Cuts at: 0 1086 5228 5569 6096 6522
Size: 1086 4142 341 527 426
Fragments arranged by size:
4142 1086 527 426 341
NdeI CA'TA_TG
Cuts at: 0 2091 6522
Size: 2091 4431
NsiI A_TGCA'T
Cuts at: 0 1568 2097 6522
Size: 1568 529 4425
Fragments arranged by size:
4425 1568 529
NspI r_CATG'y
Cuts at: 0 842 3152 3520 3738 4894 5685 6254 6522
Size: 842 2310 368 218 1156 791 569 268
Fragments arranged by size:
2310 1156 842 791 569 368 268 218
PciI A'CATG_T
Cuts at: 0 3516 3734 5681 6522
Size: 3516 218 1947 841
Fragments arranged by size:
3516 1947 841 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 4681 6522
Size: 4681 1841
PfoI T'CCnGG_A
Cuts at: 0 6078 6522
Size: 6078 444
PmlI CAC'GTG
Cuts at: 0 6309 6522
Size: 6309 213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2585 2617 6522
Size: 2585 32 3905
Fragments arranged by size:
3905 2585 32
PpuMI rG'GwC_Cy
Cuts at: 0 3916 4381 6522
Size: 3916 465 2141
Fragments arranged by size:
3916 2141 465
PshAI GACnn'nnGTC
Cuts at: 0 4379 6522
Size: 4379 2143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1840 1872 6522
Size: 1840 32 4650
Fragments arranged by size:
4650 1840 32
PstI C_TGCA'G
Cuts at: 0 798 2484 4369 4527 4707 5712 6522
Size: 798 1686 1885 158 180 1005 810
Fragments arranged by size:
1885 1686 1005 810 798 180 158
PvuII CAG'CTG
Cuts at: 0 1219 2429 4315 4783 6522
Size: 1219 1210 1886 468 1739
Fragments arranged by size:
1886 1739 1219 1210 468
SapI GCTCTTCn'nnn_
Cuts at: 0 566 3665 4058 6522
Size: 566 3099 393 2464
Fragments arranged by size:
3099 2464 566 393
ScaI AGT'ACT
Cuts at: 0 1077 5049 6126 6522
Size: 1077 3972 1077 396
Fragments arranged by size:
3972 1077 1077 396
SfcI C'TryA_G
Cuts at: 0 794 1778 1787 2480 2664 4365 4523 4703
Size: 794 984 9 693 184 1701 158 180
Cuts at: 4703 4774 5518 5708 6522
Size: 71 744 190 814
Fragments arranged by size:
1701 984 814 794 744 693 190 184
180 158 71 9
SmlI C'TyrA_G
Cuts at: 0 181 1294 3089 4345 6257 6286 6522
Size: 181 1113 1795 1256 1912 29 236
Fragments arranged by size:
1912 1795 1256 1113 236 181 29
SnaBI TAC'GTA
Cuts at: 0 412 6522
Size: 412 6110
SpeI A'CTAG_T
Cuts at: 0 2278 6522
Size: 2278 4244
SphI G_CATG'C
Cuts at: 0 842 6522
Size: 842 5680
SspI AAT'ATT
Cuts at: 0 6171 6522
Size: 6171 351
StuI AGG'CCT
Cuts at: 0 6292 6522
Size: 6292 230
StyI C'CwwG_G
Cuts at: 0 1086 1703 3034 3043 3901 4377 5228 5569
Size: 1086 617 1331 9 858 476 851 341
Cuts at: 5569 6096 6192 6522
Size: 527 96 330
Fragments arranged by size:
1331 1086 858 851 617 527 476 341
330 96 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 6371 6522
Size: 907 5464 151
Fragments arranged by size:
5464 907 151
TatI w'GTAC_w
Cuts at: 0 192 1075 4116 5047 5058 5550 5869 6069
Size: 192 883 3041 931 11 492 319 200
Cuts at: 6069 6124 6407 6522
Size: 55 283 115
Fragments arranged by size:
3041 931 883 492 319 283 200 192
115 55 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 933 965 2184 2216 3048 3080 3775 3807
Size: 933 32 1219 32 832 32 695 32
Cuts at: 3807 3980 4012 6522
Size: 173 32 2510
Fragments arranged by size:
2510 1219 933 832 695 173 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 2604 5010 6522
Size: 2604 2406 1512
Fragments arranged by size:
2604 2406 1512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4008 5937 6233 6522
Size: 4008 1929 296 289
Fragments arranged by size:
4008 1929 296 289
XmnI GAAnn'nnTTC
Cuts at: 0 712 2616 3408 6522
Size: 712 1904 792 3114
Fragments arranged by size:
3114 1904 792 712
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AvrII BaeI
BamHI BanI BanII BbsI BciVI BclI BfrBI BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PsrI PstI PvuII SapI ScaI
SfcI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI ApaI AscI AsiSI AvaI
BbeI BbvCI BcgI BglI BlpI BmgBI BmtI BplI
BsaI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BstEII BtgZI EagI EciI EcoICRI EcoNI FseI
FspI FspAI HaeII KasI KpnI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PmeI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SrfI SwaI Tth111I XhoI XmaI ZraI