Restriction map for
pFN21AE2298
(Linear) (Six-base) MAPSORT of: KIEE2298.seq Check: 679 from: 1 to: 1272
KIEE2298.orf
With 182 enzymes: SgfI *
November 10, 2010 10:12 ..
AccI GT'mk_AC
Cuts at: 0 146 482 1272
Size: 146 336 790
Fragments arranged by size:
790 336 146
AflII C'TTAA_G
Cuts at: 0 187 1272
Size: 187 1085
AflIII A'CryG_T
Cuts at: 0 251 1272
Size: 251 1021
AleI CACnn'nnGTG
Cuts at: 0 448 1272
Size: 448 824
ApoI r'AATT_y
Cuts at: 0 271 1137 1272
Size: 271 866 135
Fragments arranged by size:
866 271 135
BanII G_rGCy'C
Cuts at: 0 7 368 1187 1272
Size: 7 361 819 85
Fragments arranged by size:
819 361 85 7
BbvCI CC'TCA_GC
Cuts at: 0 692 1272
Size: 692 580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 476 1272
Size: 442 34 796
Fragments arranged by size:
796 442 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1030 1272
Size: 1030 242
BglII A'GATC_T
Cuts at: 0 333 1272
Size: 333 939
Bme1580I G_kGCm'C
Cuts at: 0 735 1172 1272
Size: 735 437 100
Fragments arranged by size:
735 437 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1272
Size: 1206 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 532 679 1272
Size: 148 384 147 593
Fragments arranged by size:
593 384 148 147
Bpu10I CC'TnA_GC
Cuts at: 0 692 1272
Size: 692 580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1272
Size: 1117 155
BsaHI Gr'CG_yC
Cuts at: 0 1214 1272
Size: 1214 58
BsaWI w'CCGG_w
Cuts at: 0 485 1272
Size: 485 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1272
Size: 991 30 251
Fragments arranged by size:
991 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 373 1272
Size: 80 293 899
Fragments arranged by size:
899 293 80
BseYI C'CCAG_C
Cuts at: 0 344 1272
Size: 344 928
BsmI GAATG_Cn'
Cuts at: 0 1057 1272
Size: 1057 215
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1257 1272
Size: 1257 15
Bsp1286I G_dGCh'C
Cuts at: 0 7 368 735 1172 1187 1272
Size: 7 361 367 437 15 85
Fragments arranged by size:
437 367 361 85 15 7
BspHI T'CATG_A
Cuts at: 0 71 1272
Size: 71 1201
BsrBI CCG'CTC
Cuts at: 0 981 1272
Size: 981 291
BsrFI r'CCGG_y
Cuts at: 0 348 1272
Size: 348 924
BstYI r'GATC_y
Cuts at: 0 333 1272
Size: 333 939
BtgI C'CryG_G
Cuts at: 0 1056 1272
Size: 1056 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1008 1272
Size: 1008 264
BtsI GCAGTG_nn'
Cuts at: 0 157 711 789 929 1272
Size: 157 554 78 140 343
Fragments arranged by size:
554 343 157 140 78
DraI TTT'AAA
Cuts at: 0 1233 1272
Size: 1233 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 1213 1272
Size: 1213 59
EaeI y'GGCC_r
Cuts at: 0 1247 1272
Size: 1247 25
EarI CTCTTCn'nnn_
Cuts at: 0 391 407 881 1272
Size: 391 16 474 391
Fragments arranged by size:
474 391 391 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1019 1272
Size: 1019 253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 532 679 1272
Size: 148 384 147 593
Fragments arranged by size:
593 384 148 147
EcoO109I rG'GnC_Cy
Cuts at: 0 974 1272
Size: 974 298
EcoRI G'AATT_C
Cuts at: 0 1137 1272
Size: 1137 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 274 306 899 931 1011 1043
Size: 112 32 130 32 593 32 80 32
Cuts at: 1043 1272
Size: 229
Fragments arranged by size:
593 229 130 112 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 147 1272
Size: 147 1125
HindIII A'AGCT_T
Cuts at: 0 1148 1272
Size: 1148 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1272
Size: 236 1036
MslI CAynn'nnrTG
Cuts at: 0 448 1272
Size: 448 824
MspA1I CmG'CkG
Cuts at: 0 657 1272
Size: 657 615
NspI r_CATG'y
Cuts at: 0 255 549 1108 1272
Size: 255 294 559 164
Fragments arranged by size:
559 294 255 164
PciI A'CATG_T
Cuts at: 0 251 1272
Size: 251 1021
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 1122 1272
Size: 657 465 150
Fragments arranged by size:
657 465 150
PfoI T'CCnGG_A
Cuts at: 0 1015 1272
Size: 1015 257
PpuMI rG'GwC_Cy
Cuts at: 0 974 1272
Size: 974 298
PvuII CAG'CTG
Cuts at: 0 657 1272
Size: 657 615
SalI G'TCGA_C
Cuts at: 0 145 1272
Size: 145 1127
SfcI C'TryA_G
Cuts at: 0 1266 1272
Size: 1266 6
SmlI C'TyrA_G
Cuts at: 0 187 1132 1272
Size: 187 945 140
Fragments arranged by size:
945 187 140
SphI G_CATG'C
Cuts at: 0 1108 1272
Size: 1108 164
StuI AGG'CCT
Cuts at: 0 1120 1272
Size: 1120 152
StyI C'CwwG_G
Cuts at: 0 230 1121 1272
Size: 230 891 151
Fragments arranged by size:
891 230 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 995 1110 1272
Size: 664 331 115 162
Fragments arranged by size:
664 331 162 115
TatI w'GTAC_w
Cuts at: 0 248 1272
Size: 248 1024
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1272
Size: 991 32 249
Fragments arranged by size:
991 249 32
Tth111I GACn'n_nGTC
Cuts at: 0 142 1272
Size: 142 1130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 1272
Size: 654 618
Enzymes that do cut:
AccI AflII AflIII AleI ApoI BanII BbvCI BcgI
BciVI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrFI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MmeI MslI MspA1I NspI PciI PflMI PfoI
PpuMI PvuII SalI SfcI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BsaI BsaAI
BsaBI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI XmnI ZraI