Restriction map for
pFN21AE2297
(Linear) (Six-base) MAPSORT of: KIEE2297.seq Check: 9431 from: 1 to: 4980
KIEE2297.orf
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1915 2052 2083 4980
Size: 1915 137 31 2897
Fragments arranged by size:
2897 1915 137 31
AccI GT'mk_AC
Cuts at: 0 122 3797 4980
Size: 122 3675 1183
Fragments arranged by size:
3675 1183 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 917 1099 1232 2785 3229 3255 3256
Size: 848 69 182 133 1553 444 26 1
Cuts at: 3256 4395 4402 4980
Size: 1139 7 578
Fragments arranged by size:
1553 1139 848 578 444 182 133 69
26 7 1
AflII C'TTAA_G
Cuts at: 0 2002 4627 4980
Size: 2002 2625 353
Fragments arranged by size:
2625 2002 353
AflIII A'CryG_T
Cuts at: 0 1813 4980
Size: 1813 3167
AhdI GACnn_n'nnGTC
Cuts at: 0 107 1474 4980
Size: 107 1367 3506
Fragments arranged by size:
3506 1367 107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4514 4546 4980
Size: 4514 32 434
Fragments arranged by size:
4514 434 32
AlwNI CAG_nnn'CTG
Cuts at: 0 322 1828 2664 3495 4328 4899 4973 4980
Size: 322 1506 836 831 833 571 74 7
Fragments arranged by size:
1506 836 833 831 571 322 74 7
ApaI G_GGCC'C
Cuts at: 0 240 4178 4980
Size: 240 3938 802
Fragments arranged by size:
3938 802 240
ApaLI G'TGCA_C
Cuts at: 0 1222 2853 4980
Size: 1222 1631 2127
Fragments arranged by size:
2127 1631 1222
ApoI r'AATT_y
Cuts at: 0 1744 4980
Size: 1744 3236
AvaI C'yCGr_G
Cuts at: 0 442 1436 1798 2908 3052 4980
Size: 442 994 362 1110 144 1928
Fragments arranged by size:
1928 1110 994 442 362 144
BamHI G'GATC_C
Cuts at: 0 4354 4980
Size: 4354 626
BanI G'GyrC_C
Cuts at: 0 54 576 1074 2321 2524 3505 4980
Size: 54 522 498 1247 203 981 1475
Fragments arranged by size:
1475 1247 981 522 498 203 54
BanII G_rGCy'C
Cuts at: 0 80 240 804 940 1148 1555 1724 1837
Size: 80 160 564 136 208 407 169 113
Cuts at: 1837 2058 2248 2423 3583 3760 4138 4178 4380
Size: 221 190 175 1160 177 378 40 202
Cuts at: 4380 4442 4856 4980
Size: 62 414 124
Fragments arranged by size:
1160 564 414 407 378 221 208 202
190 177 175 169 160 136 124 113
80 62 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 1868 2888 3471 4662 4980
Size: 353 1515 1020 583 1191 318
Fragments arranged by size:
1515 1191 1020 583 353 318
BbvCI CC'TCA_GC
Cuts at: 0 565 4433 4980
Size: 565 3868 547
Fragments arranged by size:
3868 565 547
BglI GCCn_nnn'nGGC
Cuts at: 0 205 676 2707 3097 4306 4980
Size: 205 471 2031 390 1209 674
Fragments arranged by size:
2031 1209 674 471 390 205
BglII A'GATC_T
Cuts at: 0 584 778 4980
Size: 584 194 4202
Fragments arranged by size:
4202 584 194
BlpI GC'TnA_GC
Cuts at: 0 40 3136 3815 4850 4980
Size: 40 3096 679 1035 130
Fragments arranged by size:
3096 1035 679 130 40
Bme1580I G_kGCm'C
Cuts at: 0 240 581 624 910 1226 1565 1787 2817
Size: 240 341 43 286 316 339 222 1030
Cuts at: 2817 2857 3518 3827 4178 4599 4755 4761 4840
Size: 40 661 309 351 421 156 6 79
Cuts at: 4840 4910 4980
Size: 70 70
Fragments arranged by size:
1030 661 421 351 341 339 316 309
286 240 222 156 79 70 70 43
40 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 113 147 780 1391 3881 4980
Size: 83 30 34 633 611 2490 1099
Fragments arranged by size:
2490 1099 633 611 83 34 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 1095 1142 2425 3330 4132 4980
Size: 348 747 47 1283 905 802 848
Fragments arranged by size:
1283 905 848 802 747 348 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 854 905 1003 1373 1593 2314 2571
Size: 472 382 51 98 370 220 721 257
Cuts at: 2571 3190 4127 4438 4980
Size: 619 937 311 542
Fragments arranged by size:
937 721 619 542 472 382 370 311
257 220 98 51
Bpu10I CC'TnA_GC
Cuts at: 0 241 565 625 1489 3577 4433 4980
Size: 241 324 60 864 2088 856 547
Fragments arranged by size:
2088 864 856 547 324 241 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3232 4980
Size: 3232 1748
BsaBI GATnn'nnATC
Cuts at: 0 2359 4980
Size: 2359 2621
BsaHI Gr'CG_yC
Cuts at: 0 3045 3638 4980
Size: 3045 593 1342
Fragments arranged by size:
3045 1342 593
BsaWI w'CCGG_w
Cuts at: 0 2204 2435 2483 2645 3144 4980
Size: 2204 231 48 162 499 1836
Fragments arranged by size:
2204 1836 499 231 162 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 1006 1216 1246 1346 1376 2164 2194
Size: 976 30 210 30 100 30 788 30
Cuts at: 2194 3259 3289 4384 4414 4492 4495 4522 4525
Size: 1065 30 1095 30 78 3 27 3
Cuts at: 4525 4980
Size: 455
Fragments arranged by size:
1095 1065 976 788 455 210 100 78
30 30 30 30 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 553 684 1936 2188 2311 2989 3644
Size: 505 48 131 1252 252 123 678 655
Cuts at: 3644 4297 4405 4435 4450 4480 4504 4513 4516
Size: 653 108 30 15 30 24 9 3
Cuts at: 4516 4783 4980
Size: 267 197
Fragments arranged by size:
1252 678 655 653 505 267 252 197
131 123 108 48 30 30 24 15
9 3
BseYI C'CCAG_C
Cuts at: 0 96 156 289 540 1497 1651 1821 3224
Size: 96 60 133 251 957 154 170 1403
Cuts at: 3224 3321 3394 3616 3959 4171 4177 4203 4444
Size: 97 73 222 343 212 6 26 241
Cuts at: 4444 4566 4782 4856 4980
Size: 122 216 74 124
Fragments arranged by size:
1403 957 343 251 241 222 216 212
170 154 133 124 122 97 96 74
73 60 26 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 2850 3550 4594 4980
Size: 1534 1316 700 1044 386
Fragments arranged by size:
1534 1316 1044 700 386
BsiEI CG_ry'CG
Cuts at: 0 2150 4980
Size: 2150 2830
BsiHKAI G_wGCw'C
Cuts at: 0 836 940 1226 1724 1898 2248 2423 2645
Size: 836 104 286 498 174 350 175 222
Cuts at: 2645 2857 3020 3142 3760 4980
Size: 212 163 122 618 1220
Fragments arranged by size:
1220 836 618 498 350 286 222 212
175 174 163 122 104
BsmI GAATG_Cn'
Cuts at: 0 433 2384 2991 4980
Size: 433 1951 607 1989
Fragments arranged by size:
1989 1951 607 433
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3824 4980
Size: 3824 1156
Bsp1286I G_dGCh'C
Cuts at: 0 80 240 581 624 804 836 910 940
Size: 80 160 341 43 180 32 74 30
Cuts at: 940 1148 1226 1555 1565 1724 1787 1837 1898
Size: 208 78 329 10 159 63 50 61
Cuts at: 1898 2058 2248 2423 2645 2817 2857 3020 3142
Size: 160 190 175 222 172 40 163 122
Cuts at: 3142 3518 3583 3760 3827 4138 4178 4380 4442
Size: 376 65 177 67 311 40 202 62
Cuts at: 4442 4599 4755 4761 4840 4856 4910 4980
Size: 157 156 6 79 16 54 70
Fragments arranged by size:
376 341 329 311 222 208 202 190
180 177 175 172 163 160 160 159
157 156 122 80 79 78 74 70
67 65 63 62 61 54 50 43
40 40 32 30 16 10 6
BspEI T'CCGG_A
Cuts at: 0 2204 2435 2645 3144 4980
Size: 2204 231 210 499 1836
Fragments arranged by size:
2204 1836 499 231 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1915 2052 2083 2497 4980
Size: 1915 137 31 414 2483
Fragments arranged by size:
2483 1915 414 137 31
BsrBI CCG'CTC
Cuts at: 0 495 2178 4456 4980
Size: 495 1683 2278 524
Fragments arranged by size:
2278 1683 524 495
BsrDI GCAATG_nn'
Cuts at: 0 629 665 2261 4980
Size: 629 36 1596 2719
Fragments arranged by size:
2719 1596 629 36
BsrFI r'CCGG_y
Cuts at: 0 57 532 1527 3332 4428 4980
Size: 57 475 995 1805 1096 552
Fragments arranged by size:
1805 1096 995 552 475 57
BsrGI T'GTAC_A
Cuts at: 0 2687 2939 4980
Size: 2687 252 2041
Fragments arranged by size:
2687 2041 252
BstEII G'GTnAC_C
Cuts at: 0 2724 4923 4980
Size: 2724 2199 57
Fragments arranged by size:
2724 2199 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 4032 4317 4980
Size: 96 3936 285 663
Fragments arranged by size:
3936 663 285 96
BstYI r'GATC_y
Cuts at: 0 584 778 2134 3775 4354 4980
Size: 584 194 1356 1641 579 626
Fragments arranged by size:
1641 1356 626 584 579 194
Bsu36I CC'TnA_GG
Cuts at: 0 48 323 749 1037 2705 4980
Size: 48 275 426 288 1668 2275
Fragments arranged by size:
2275 1668 426 288 275 48
BtgI C'CryG_G
Cuts at: 0 21 971 1297 1361 2144 2250 2858 4129
Size: 21 950 326 64 783 106 608 1271
Cuts at: 4129 4195 4559 4572 4704 4980
Size: 66 364 13 132 276
Fragments arranged by size:
1271 950 783 608 364 326 276 132
106 66 64 21 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 4980
Size: 431 4549
BtsI GCAGTG_nn'
Cuts at: 0 358 2337 3534 4980
Size: 358 1979 1197 1446
Fragments arranged by size:
1979 1446 1197 358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2502 2537 4980
Size: 2502 35 2443
Fragments arranged by size:
2502 2443 35
DraIII CAC_nnn'GTG
Cuts at: 0 3726 4980
Size: 3726 1254
DrdI GACnn_nn'nnGTC
Cuts at: 0 3858 4921 4980
Size: 3858 1063 59
Fragments arranged by size:
3858 1063 59
EaeI y'GGCC_r
Cuts at: 0 212 313 2666 2948 4056 4556 4980
Size: 212 101 2353 282 1108 500 424
Fragments arranged by size:
2353 1108 500 424 282 212 101
EarI CTCTTCn'nnn_
Cuts at: 0 824 1086 1213 1459 1697 1712 2644 4490
Size: 824 262 127 246 238 15 932 1846
Cuts at: 4490 4980
Size: 490
Fragments arranged by size:
1846 932 824 490 262 246 238 127
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2596 4759 4980
Size: 2596 2163 221
Fragments arranged by size:
2596 2163 221
EcoICRI GAG'CTC
Cuts at: 0 938 1722 2246 2421 3758 4980
Size: 938 784 524 175 1337 1222
Fragments arranged by size:
1337 1222 938 784 524 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 848 854 905 917 1003 1099 1232
Size: 472 376 6 51 12 86 96 133
Cuts at: 1232 1373 1593 2314 2571 2785 3190 3229 3255
Size: 141 220 721 257 214 405 39 26
Cuts at: 3255 3256 4127 4395 4402 4438 4980
Size: 1 871 268 7 36 542
Fragments arranged by size:
871 721 542 472 405 376 268 257
220 214 141 133 96 86 51 39
36 26 12 7 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 748 1216 2413 2875 4420 4980
Size: 748 468 1197 462 1545 560
Fragments arranged by size:
1545 1197 748 560 468 462
EcoO109I rG'GnC_Cy
Cuts at: 0 109 236 237 307 319 511 600 678
Size: 109 127 1 70 12 192 89 78
Cuts at: 678 1041 1357 1433 1493 1646 1794 2661 3165
Size: 363 316 76 60 153 148 867 504
Cuts at: 3165 3499 3682 3708 3735 3883 4325 4404 4980
Size: 334 183 26 27 148 442 79 576
Fragments arranged by size:
867 576 504 442 363 334 316 192
183 153 148 148 127 109 89 79
78 76 70 60 27 26 12 1
FseI GG_CCGG'CC
Cuts at: 0 536 4980
Size: 536 4444
HaeII r_GCGC'y
Cuts at: 0 47 1004 4204 4980
Size: 47 957 3200 776
Fragments arranged by size:
3200 957 776 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 742 774 823 855 1460 1492
Size: 93 32 617 32 49 32 605 32
Cuts at: 1492 2456 2488 4207 4239 4495 4527 4980
Size: 964 32 1719 32 256 32 453
Fragments arranged by size:
1719 964 617 605 453 256 93 49
32 32 32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1491 3066 3170 3365 3785 4493 4980
Size: 1491 1575 104 195 420 708 487
Fragments arranged by size:
1575 1491 708 487 420 195 104
MscI TGG'CCA
Cuts at: 0 2668 2950 4058 4980
Size: 2668 282 1108 922
Fragments arranged by size:
2668 1108 922 282
MslI CAynn'nnrTG
Cuts at: 0 3013 4980
Size: 3013 1967
MspA1I CmG'CkG
Cuts at: 0 82 1351 2252 2922 3002 3078 3084 3290
Size: 82 1269 901 670 80 76 6 206
Cuts at: 3290 3804 3959 4028 4317 4643 4740 4973 4980
Size: 514 155 69 289 326 97 233 7
Fragments arranged by size:
1269 901 670 514 326 289 233 206
155 97 82 80 76 69 7 6
NaeI GCC'GGC
Cuts at: 0 534 3334 4430 4980
Size: 534 2800 1096 550
Fragments arranged by size:
2800 1096 550 534
NcoI C'CATG_G
Cuts at: 0 21 971 4980
Size: 21 950 4009
Fragments arranged by size:
4009 950 21
NgoMIV G'CCGG_C
Cuts at: 0 532 3332 4428 4980
Size: 532 2800 1096 552
Fragments arranged by size:
2800 1096 552 532
NspI r_CATG'y
Cuts at: 0 1817 2559 2929 3927 4980
Size: 1817 742 370 998 1053
Fragments arranged by size:
1817 1053 998 742 370
PasI CC'CwG_GG
Cuts at: 0 508 1384 4350 4980
Size: 508 876 2966 630
Fragments arranged by size:
2966 876 630 508
PciI A'CATG_T
Cuts at: 0 1813 4980
Size: 1813 3167
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 2664 4710 4980
Size: 192 2472 2046 270
Fragments arranged by size:
2472 2046 270 192
PfoI T'CCnGG_A
Cuts at: 0 1478 2159 2548 2657 4532 4980
Size: 1478 681 389 109 1875 448
Fragments arranged by size:
1875 1478 681 448 389 109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4144 4176 4980
Size: 4144 32 804
Fragments arranged by size:
4144 804 32
PpuMI rG'GwC_Cy
Cuts at: 0 109 307 1357 1493 1646 1794 2661 3499
Size: 109 198 1050 136 153 148 867 838
Cuts at: 3499 3735 3883 4404 4980
Size: 236 148 521 576
Fragments arranged by size:
1050 867 838 576 521 236 198 153
148 148 136 109
PshAI GACnn'nnGTC
Cuts at: 0 2753 3406 4980
Size: 2753 653 1574
Fragments arranged by size:
2753 1574 653
PspOMI G'GGCC_C
Cuts at: 0 236 4174 4980
Size: 236 3938 806
Fragments arranged by size:
3938 806 236
PspXI vC'TCGA_Gb
Cuts at: 0 442 4980
Size: 442 4538
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3349 3381 4980
Size: 3349 32 1599
Fragments arranged by size:
3349 1599 32
PstI C_TGCA'G
Cuts at: 0 365 1181 4980
Size: 365 816 3799
Fragments arranged by size:
3799 816 365
PvuII CAG'CTG
Cuts at: 0 1351 2922 3084 3959 4028 4317 4740 4980
Size: 1351 1571 162 875 69 289 423 240
Fragments arranged by size:
1571 1351 875 423 289 240 162 69
SacI G_AGCT'C
Cuts at: 0 940 1724 2248 2423 3760 4980
Size: 940 784 524 175 1337 1220
Fragments arranged by size:
1337 1220 940 784 524 175
SacII CC_GC'GG
Cuts at: 0 2253 4980
Size: 2253 2727
SanDI GG'GwC_CC
Cuts at: 0 307 3735 4404 4980
Size: 307 3428 669 576
Fragments arranged by size:
3428 669 576 307
SapI GCTCTTCn'nnn_
Cuts at: 0 824 1712 4980
Size: 824 888 3268
Fragments arranged by size:
3268 888 824
ScaI AGT'ACT
Cuts at: 0 3907 4980
Size: 3907 1073
SexAI A'CCwGG_T
Cuts at: 0 2720 4980
Size: 2720 2260
SfcI C'TryA_G
Cuts at: 0 361 1177 4965 4980
Size: 361 816 3788 15
Fragments arranged by size:
3788 816 361 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2707 4980
Size: 2707 2273
SmaI CCC'GGG
Cuts at: 0 1438 4980
Size: 1438 3542
SmlI C'TyrA_G
Cuts at: 0 442 2002 3247 4627 4980
Size: 442 1560 1245 1380 353
Fragments arranged by size:
1560 1380 1245 442 353
StuI AGG'CCT
Cuts at: 0 754 2710 3200 3233 4980
Size: 754 1956 490 33 1747
Fragments arranged by size:
1956 1747 754 490 33
StyI C'CwwG_G
Cuts at: 0 21 112 635 971 1058 1319 1949 2499
Size: 21 91 523 336 87 261 630 550
Cuts at: 2499 2876 3686 3704 4166 4980
Size: 377 810 18 462 814
Fragments arranged by size:
814 810 630 550 523 462 377 336
261 91 87 21 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 1168 1332 1959 1986 2034 2262 2840
Size: 417 751 164 627 27 48 228 578
Cuts at: 2840 3940 4980
Size: 1100 1040
Fragments arranged by size:
1100 1040 751 627 578 417 228 164
48 27
TatI w'GTAC_w
Cuts at: 0 1110 2558 2687 2939 3284 3365 3905 4980
Size: 1110 1448 129 252 345 81 540 1075
Fragments arranged by size:
1448 1110 1075 540 345 252 129 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1346 1378 3259 3291 3712 3744
Size: 1216 32 98 32 1881 32 421 32
Cuts at: 3744 3930 3962 4384 4416 4492 4524 4980
Size: 186 32 422 32 76 32 456
Fragments arranged by size:
1881 1216 456 422 421 186 98 76
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 89 3887 4980
Size: 89 3798 1093
Fragments arranged by size:
3798 1093 89
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 1065 2031 4051 4707 4980
Size: 978 87 966 2020 656 273
Fragments arranged by size:
2020 978 966 656 273 87
XhoI C'TCGA_G
Cuts at: 0 442 4980
Size: 442 4538
XmaI C'CCGG_G
Cuts at: 0 1436 4980
Size: 1436 3544
XmnI GAAnn'nnTTC
Cuts at: 0 2653 3268 4980
Size: 2653 615 1712
Fragments arranged by size:
2653 1712 615
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII SacI SacII SanDI SapI
ScaI SexAI SfcI SfiI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AleI AlfI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI
BfrBI BmgBI BmtI BsaI BsaAI BsiWI BspHI BssHII
BssSI BstAPI BstBI BstZ17I ClaI DraI EagI EcoRI
EcoRV FalI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PsiI PvuI RsrII SalI
SbfI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI ZraI