Restriction map for
pFN21AE2295
(Linear) (Six-base) MAPSORT of: KIEE2295.seq Check: 1586 from: 1 to: 6408
KIEE2295.orf
With 182 enzymes: SgfI *
November 8, 2010 13:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4270 5952 6045 6408
Size: 4270 1682 93 363
Fragments arranged by size:
4270 1682 363 93
AccI GT'mk_AC
Cuts at: 0 958 2544 6408
Size: 958 1586 3864
Fragments arranged by size:
3864 1586 958
AclI AA'CG_TT
Cuts at: 0 2462 6408
Size: 2462 3946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 2237 5450 5549 5605 5912 6308 6408
Size: 707 1530 3213 99 56 307 396 100
Fragments arranged by size:
3213 1530 707 396 307 100 99 56
AflII C'TTAA_G
Cuts at: 0 838 6408
Size: 838 5570
AflIII A'CryG_T
Cuts at: 0 2283 3325 4684 5233 6378 6408
Size: 2283 1042 1359 549 1145 30
Fragments arranged by size:
2283 1359 1145 1042 549 30
AgeI A'CCGG_T
Cuts at: 0 4414 6408
Size: 4414 1994
AhdI GACnn_n'nnGTC
Cuts at: 0 1381 5937 6408
Size: 1381 4556 471
Fragments arranged by size:
4556 1381 471
AleI CACnn'nnGTG
Cuts at: 0 1678 1681 5785 6408
Size: 1678 3 4104 623
Fragments arranged by size:
4104 1678 623 3
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 2226 2258 2600 2632 6408
Size: 268 32 1926 32 342 32 3776
Fragments arranged by size:
3776 1926 342 268 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2197 3153 3999 5683 6408
Size: 2197 956 846 1684 725
Fragments arranged by size:
2197 1684 956 846 725
ApaI G_GGCC'C
Cuts at: 0 6392 6408
Size: 6392 16
ApaLI G'TGCA_C
Cuts at: 0 4169 6408
Size: 4169 2239
ApoI r'AATT_y
Cuts at: 0 246 982 1455 2326 2341 2374 2718 3000
Size: 246 736 473 871 15 33 344 282
Cuts at: 3000 3030 3580 3588 3635 4150 4179 6308 6408
Size: 30 550 8 47 515 29 2129 100
Fragments arranged by size:
2129 871 736 550 515 473 344 282
246 100 47 33 30 29 15 8
AseI AT'TA_AT
Cuts at: 0 154 5765 6408
Size: 154 5611 643
Fragments arranged by size:
5611 643 154
AvaI C'yCGr_G
Cuts at: 0 4934 5066 6408
Size: 4934 132 1342
Fragments arranged by size:
4934 1342 132
AvrII C'CTAG_G
Cuts at: 0 1092 6408
Size: 1092 5316
BamHI G'GATC_C
Cuts at: 0 861 6408
Size: 861 5547
BanI G'GyrC_C
Cuts at: 0 4637 5211 5668 6408
Size: 4637 574 457 740
Fragments arranged by size:
4637 740 574 457
BanII G_rGCy'C
Cuts at: 0 1355 1417 1636 2416 4610 5759 6392 6408
Size: 1355 62 219 780 2194 1149 633 16
Fragments arranged by size:
2194 1355 1149 780 633 219 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1284 1804 2102 5563 5664 6408
Size: 1284 520 298 3461 101 744
Fragments arranged by size:
3461 1284 744 520 298 101
BbvCI CC'TCA_GC
Cuts at: 0 5948 6408
Size: 5948 460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1070 6408
Size: 1036 34 5338
Fragments arranged by size:
5338 1036 34
BclI T'GATC_A
Cuts at: 0 2157 2439 2559 6408
Size: 2157 282 120 3849
Fragments arranged by size:
3849 2157 282 120
BfrBI ATG'CAT
Cuts at: 0 3990 6408
Size: 3990 2418
BglI GCCn_nnn'nGGC
Cuts at: 0 2683 6408
Size: 2683 3725
BglII A'GATC_T
Cuts at: 0 1179 1850 6408
Size: 1179 671 4558
Fragments arranged by size:
4558 1179 671
Bme1580I G_kGCm'C
Cuts at: 0 3364 4173 4640 6069 6392 6408
Size: 3364 809 467 1429 323 16
Fragments arranged by size:
3364 1429 809 467 323 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1141 1591 5512 6408
Size: 1141 450 3921 896
Fragments arranged by size:
3921 1141 896 450
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2739 5175 6408
Size: 2739 2436 1233
Fragments arranged by size:
2739 2436 1233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 882 1451 1930 4877 5519 6408
Size: 251 631 569 479 2947 642 889
Fragments arranged by size:
2947 889 642 631 569 479 251
Bpu10I CC'TnA_GC
Cuts at: 0 731 1349 1416 5948 6408
Size: 731 618 67 4532 460
Fragments arranged by size:
4532 731 618 460 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 2431 3050 5084 5831 6408
Size: 770 1661 619 2034 747 577
Fragments arranged by size:
2034 1661 770 747 619 577
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 5410 6408
Size: 1165 4245 998
Fragments arranged by size:
4245 1165 998
BsaAI yAC'GTr
Cuts at: 0 2163 6408
Size: 2163 4245
BsaBI GATnn'nnATC
Cuts at: 0 604 3104 6408
Size: 604 2500 3304
Fragments arranged by size:
3304 2500 604
BsaWI w'CCGG_w
Cuts at: 0 4038 4414 6408
Size: 4038 376 1994
Fragments arranged by size:
4038 1994 376
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 880 910 2226 2256 4351 4381 5562 5592
Size: 880 30 1316 30 2095 30 1181 30
Cuts at: 5592 5812 5842 6408
Size: 220 30 566
Fragments arranged by size:
2095 1316 1181 880 566 220 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1418 1704 1888 3897 5501 5714 5834 5965
Size: 1418 286 184 2009 1604 213 120 131
Cuts at: 5965 6408
Size: 443
Fragments arranged by size:
2009 1604 1418 443 286 213 184 131
120
BseYI C'CCAG_C
Cuts at: 0 796 3899 6408
Size: 796 3103 2509
Fragments arranged by size:
3103 2509 796
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6064 6408
Size: 6064 344
BsiEI CG_ry'CG
Cuts at: 0 4593 6408
Size: 4593 1815
BsiHKAI G_wGCw'C
Cuts at: 0 750 1636 2416 4173 4553 4610 5007 5996
Size: 750 886 780 1757 380 57 397 989
Cuts at: 5996 6104 6408
Size: 108 304
Fragments arranged by size:
1757 989 886 780 750 397 380 304
108 57
BsmI GAATG_Cn'
Cuts at: 0 800 1819 3826 3876 5886 6408
Size: 800 1019 2007 50 2010 522
Fragments arranged by size:
2010 2007 1019 800 522 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 968 6408
Size: 968 5440
Bsp1286I G_dGCh'C
Cuts at: 0 750 1355 1417 1636 2416 3364 4173 4553
Size: 750 605 62 219 780 948 809 380
Cuts at: 4553 4610 4640 5007 5759 5996 6069 6104 6392
Size: 57 30 367 752 237 73 35 288
Cuts at: 6392 6408
Size: 16
Fragments arranged by size:
948 809 780 752 750 605 380 367
288 237 219 73 62 57 35 30
16
BspHI T'CATG_A
Cuts at: 0 4023 6408
Size: 4023 2385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 301 1494 3957 4270 5952 6045 6408
Size: 301 1193 2463 313 1682 93 363
Fragments arranged by size:
2463 1682 1193 363 313 301 93
BsrBI CCG'CTC
Cuts at: 0 5081 5509 6408
Size: 5081 428 899
Fragments arranged by size:
5081 899 428
BsrDI GCAATG_nn'
Cuts at: 0 303 1491 4006 6408
Size: 303 1188 2515 2402
Fragments arranged by size:
2515 2402 1188 303
BsrFI r'CCGG_y
Cuts at: 0 4414 4592 6408
Size: 4414 178 1816
Fragments arranged by size:
4414 1816 178
BsrGI T'GTAC_A
Cuts at: 0 522 2256 2286 3333 3390 4409 4681 6408
Size: 522 1734 30 1047 57 1019 272 1727
Fragments arranged by size:
1734 1727 1047 1019 522 272 57 30
BssSI C'ACGA_G
Cuts at: 0 824 6408
Size: 824 5584
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1823 3111 3557 3987 5683 6408
Size: 1823 1288 446 430 1696 725
Fragments arranged by size:
1823 1696 1288 725 446 430
BstBI TT'CG_AA
Cuts at: 0 1046 6408
Size: 1046 5362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1124 2482 6408
Size: 1124 1358 3926
Fragments arranged by size:
3926 1358 1124
BstYI r'GATC_y
Cuts at: 0 635 861 1179 1850 2608 6408
Size: 635 226 318 671 758 3800
Fragments arranged by size:
3800 758 671 635 318 226
BstZ17I GTA'TAC
Cuts at: 0 2545 6408
Size: 2545 3863
Bsu36I CC'TnA_GG
Cuts at: 0 5400 6408
Size: 5400 1008
BtgI C'CryG_G
Cuts at: 0 2012 6408
Size: 2012 4396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1782 3959 5293 6015 6408
Size: 1782 2177 1334 722 393
Fragments arranged by size:
2177 1782 1334 722 393
BtsI GCAGTG_nn'
Cuts at: 0 2387 4482 4982 5321 5389 6408
Size: 2387 2095 500 339 68 1019
Fragments arranged by size:
2387 2095 1019 500 339 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6078 6113 6408
Size: 6078 35 295
Fragments arranged by size:
6078 295 35
DraI TTT'AAA
Cuts at: 0 1964 2322 6408
Size: 1964 358 4086
Fragments arranged by size:
4086 1964 358
DraIII CAC_nnn'GTG
Cuts at: 0 667 743 5520 6408
Size: 667 76 4777 888
Fragments arranged by size:
4777 888 667 76
DrdI GACnn_nn'nnGTC
Cuts at: 0 490 1288 6349 6408
Size: 490 798 5061 59
Fragments arranged by size:
5061 798 490 59
EaeI y'GGCC_r
Cuts at: 0 4586 6026 6408
Size: 4586 1440 382
Fragments arranged by size:
4586 1440 382
EarI CTCTTCn'nnn_
Cuts at: 0 683 806 1573 1722 4109 4583 5263 5865
Size: 683 123 767 149 2387 474 680 602
Cuts at: 5865 6408
Size: 543
Fragments arranged by size:
2387 767 683 680 602 543 474 149
123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3489 6408
Size: 3489 2919
EcoICRI GAG'CTC
Cuts at: 0 1634 2414 4608 6408
Size: 1634 780 2194 1800
Fragments arranged by size:
2194 1800 1634 780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 707 882 1451 1930 2237 4877 5450
Size: 251 456 175 569 479 307 2640 573
Cuts at: 5450 5519 5549 5605 5912 6308 6408
Size: 69 30 56 307 396 100
Fragments arranged by size:
2640 573 569 479 456 396 307 307
251 175 100 69 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1096 1559 6408
Size: 1096 463 4849
Fragments arranged by size:
4849 1096 463
EcoO109I rG'GnC_Cy
Cuts at: 0 1161 1934 2685 5205 6388 6389 6408
Size: 1161 773 751 2520 1183 1 19
Fragments arranged by size:
2520 1183 1161 773 751 19 1
EcoRI G'AATT_C
Cuts at: 0 2341 2718 6408
Size: 2341 377 3690
Fragments arranged by size:
3690 2341 377
EcoRV GAT'ATC
Cuts at: 0 3739 6408
Size: 3739 2669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1030 1270 6408
Size: 1030 240 5138
Fragments arranged by size:
5138 1030 240
HaeII r_GCGC'y
Cuts at: 0 316 6408
Size: 316 6092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 1164 1196 1367 1399 1760 1792
Size: 1069 32 63 32 171 32 361 32
Cuts at: 1792 2424 2456 2737 2769 3063 3095 3172 3204
Size: 632 32 281 32 294 32 77 32
Cuts at: 3204 4646 4678 4795 4827 5065 5097 5842 5874
Size: 1442 32 117 32 238 32 745 32
Cuts at: 5874 5893 5923 5925 5955 5965 5997 6408
Size: 19 30 2 30 10 32 411
Fragments arranged by size:
1442 1069 745 632 411 361 294 281
238 171 117 77 63 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 19 10 2
HincII GTy'rAC
Cuts at: 0 1671 2153 2223 3242 3863 6408
Size: 1671 482 70 1019 621 2545
Fragments arranged by size:
2545 1671 1019 621 482 70
HindIII A'AGCT_T
Cuts at: 0 1515 3839 5134 6408
Size: 1515 2324 1295 1274
Fragments arranged by size:
2324 1515 1295 1274
HpaI GTT'AAC
Cuts at: 0 2153 3863 6408
Size: 2153 1710 2545
Fragments arranged by size:
2545 2153 1710
MfeI C'AATT_G
Cuts at: 0 1316 3087 4448 6408
Size: 1316 1771 1361 1960
Fragments arranged by size:
1960 1771 1361 1316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2957 3839 4555 6145 6408
Size: 2957 882 716 1590 263
Fragments arranged by size:
2957 1590 882 716 263
MscI TGG'CCA
Cuts at: 0 6028 6408
Size: 6028 380
MslI CAynn'nnrTG
Cuts at: 0 416 1122 1678 1681 2116 4558 5785 5969
Size: 416 706 556 3 435 2442 1227 184
Cuts at: 5969 6344 6408
Size: 375 64
Fragments arranged by size:
2442 1227 706 556 435 416 375 184
64 3
MspA1I CmG'CkG
Cuts at: 0 631 2302 4268 5680 6408
Size: 631 1671 1966 1412 728
Fragments arranged by size:
1966 1671 1412 728 631
NcoI C'CATG_G
Cuts at: 0 2012 6408
Size: 2012 4396
NdeI CA'TA_TG
Cuts at: 0 1371 6408
Size: 1371 5037
NsiI A_TGCA'T
Cuts at: 0 3992 6408
Size: 3992 2416
NspI r_CATG'y
Cuts at: 0 2287 3329 4688 6408
Size: 2287 1042 1359 1720
Fragments arranged by size:
2287 1720 1359 1042
PasI CC'CwG_GG
Cuts at: 0 1164 6408
Size: 1164 5244
PciI A'CATG_T
Cuts at: 0 2283 3325 4684 6408
Size: 2283 1042 1359 1724
Fragments arranged by size:
2283 1724 1359 1042
PflMI CCAn_nnn'nTGG
Cuts at: 0 1153 1993 2605 3153 3656 6408
Size: 1153 840 612 548 503 2752
Fragments arranged by size:
2752 1153 840 612 548 503
PmlI CAC'GTG
Cuts at: 0 2163 6408
Size: 2163 4245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2226 2258 6408
Size: 2226 32 4150
Fragments arranged by size:
4150 2226 32
PpuMI rG'GwC_Cy
Cuts at: 0 5205 6408
Size: 5205 1203
PsiI TTA'TAA
Cuts at: 0 425 1918 2372 3845 4880 5132 6408
Size: 425 1493 454 1473 1035 252 1276
Fragments arranged by size:
1493 1473 1276 1035 454 425 252
PspOMI G'GGCC_C
Cuts at: 0 6388 6408
Size: 6388 20
PstI C_TGCA'G
Cuts at: 0 630 2497 4267 6408
Size: 630 1867 1770 2141
Fragments arranged by size:
2141 1867 1770 630
PvuII CAG'CTG
Cuts at: 0 631 2302 4268 5680 6408
Size: 631 1671 1966 1412 728
Fragments arranged by size:
1966 1671 1412 728 631
SacI G_AGCT'C
Cuts at: 0 1636 2416 4610 6408
Size: 1636 780 2194 1798
Fragments arranged by size:
2194 1798 1636 780
SfcI C'TryA_G
Cuts at: 0 626 2019 2493 4263 6408
Size: 626 1393 474 1770 2145
Fragments arranged by size:
2145 1770 1393 626 474
SmlI C'TyrA_G
Cuts at: 0 785 838 2446 3065 5066 5099 5846 6408
Size: 785 53 1608 619 2001 33 747 562
Fragments arranged by size:
2001 1608 785 747 619 562 53 33
SspI AAT'ATT
Cuts at: 0 2334 2986 6015 6408
Size: 2334 652 3029 393
Fragments arranged by size:
3029 2334 652 393
StuI AGG'CCT
Cuts at: 0 24 1042 1102 6238 6408
Size: 24 1018 60 5136 170
Fragments arranged by size:
5136 1018 170 60 24
StyI C'CwwG_G
Cuts at: 0 92 1014 1092 1212 2012 4317 5162 6408
Size: 92 922 78 120 800 2305 845 1246
Fragments arranged by size:
2305 1246 922 845 800 120 92 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 1156 4430 5077 5531 6408
Size: 502 654 3274 647 454 877
Fragments arranged by size:
3274 877 654 647 502 454
TatI w'GTAC_w
Cuts at: 0 522 2256 2286 3304 3333 3390 4409 4681
Size: 522 1734 30 1018 29 57 1019 272
Cuts at: 4681 4748 6408
Size: 67 1660
Fragments arranged by size:
1734 1660 1019 1018 522 272 67 57
30 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 651 683 727 759 4079 4111 5033 5065
Size: 651 32 44 32 3320 32 922 32
Cuts at: 5065 5562 5594 5812 5844 6408
Size: 497 32 218 32 564
Fragments arranged by size:
3320 922 651 564 497 218 44 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 4454 5169 5205 5543 6408
Size: 108 4346 715 36 338 865
Fragments arranged by size:
4346 865 715 338 108 36
XhoI C'TCGA_G
Cuts at: 0 5066 6408
Size: 5066 1342
XmnI GAAnn'nnTTC
Cuts at: 0 3826 4490 6408
Size: 3826 664 1918
Fragments arranged by size:
3826 1918 664
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BclI
BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PasI PciI PflMI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuII SacI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AlfI AscI AsiSI BaeI BbeI
BciVI BlpI BmgBI BmtI BsaHI BsiWI BspEI BssHII
BstEII ClaI EagI FseI FspI FspAI KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PfoI PmeI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XmaI ZraI