Restriction map for
pFN21AE2292
(Linear) (Six-base) MAPSORT of: KIEE2292.seq Check: 5081 from: 1 to: 984
KIEE2292.orf
With 182 enzymes: SgfI *
July 15, 2010 18:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 984
Size: 707 277
AfeI AGC'GCT
Cuts at: 0 367 984
Size: 367 617
AflIII A'CryG_T
Cuts at: 0 572 574 984
Size: 572 2 410
Fragments arranged by size:
572 410 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 650 984
Size: 650 334
AlwNI CAG_nnn'CTG
Cuts at: 0 443 984
Size: 443 541
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 713 759 984
Size: 311 402 46 225
Fragments arranged by size:
402 311 225 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 367 401 984
Size: 367 34 583
Fragments arranged by size:
583 367 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 245 984
Size: 245 739
BlpI GC'TnA_GC
Cuts at: 0 5 679 984
Size: 5 674 305
Fragments arranged by size:
674 305 5
BsaAI yAC'GTr
Cuts at: 0 575 984
Size: 575 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 984
Size: 92 30 862
Fragments arranged by size:
862 92 30
BseYI C'CCAG_C
Cuts at: 0 812 984
Size: 812 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 925 984
Size: 656 269 59
Fragments arranged by size:
656 269 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 984
Size: 383 601
BsrDI GCAATG_nn'
Cuts at: 0 152 653 984
Size: 152 501 331
Fragments arranged by size:
501 331 152
BsrGI T'GTAC_A
Cuts at: 0 577 984
Size: 577 407
BstYI r'GATC_y
Cuts at: 0 20 984
Size: 20 964
Bsu36I CC'TnA_GG
Cuts at: 0 261 551 984
Size: 261 290 433
Fragments arranged by size:
433 290 261
BtgI C'CryG_G
Cuts at: 0 136 984
Size: 136 848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 627 984
Size: 627 357
EaeI y'GGCC_r
Cuts at: 0 46 984
Size: 46 938
EarI CTCTTCn'nnn_
Cuts at: 0 512 869 984
Size: 512 357 115
Fragments arranged by size:
512 357 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 984
Size: 707 277
HaeII r_GCGC'y
Cuts at: 0 369 984
Size: 369 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 601 633 751 783 984
Size: 92 32 477 32 118 32 201
Fragments arranged by size:
477 201 118 92 32 32 32
MfeI C'AATT_G
Cuts at: 0 498 984
Size: 498 486
MslI CAynn'nnrTG
Cuts at: 0 459 646 984
Size: 459 187 338
Fragments arranged by size:
459 338 187
MspA1I CmG'CkG
Cuts at: 0 58 984
Size: 58 926
NspI r_CATG'y
Cuts at: 0 259 464 984
Size: 259 205 520
Fragments arranged by size:
520 259 205
PasI CC'CwG_GG
Cuts at: 0 510 984
Size: 510 474
PmlI CAC'GTG
Cuts at: 0 575 984
Size: 575 409
PstI C_TGCA'G
Cuts at: 0 851 984
Size: 851 133
PvuII CAG'CTG
Cuts at: 0 58 984
Size: 58 926
SapI GCTCTTCn'nnn_
Cuts at: 0 869 984
Size: 869 115
SfcI C'TryA_G
Cuts at: 0 467 847 984
Size: 467 380 137
Fragments arranged by size:
467 380 137
StyI C'CwwG_G
Cuts at: 0 854 869 984
Size: 854 15 115
Fragments arranged by size:
854 115 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 984
Size: 826 158
TatI w'GTAC_w
Cuts at: 0 577 984
Size: 577 407
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI BbsI BcgI BciVI
BlpI BsaAI BsaXI BseYI BsgI BspMI BsrDI BsrGI
BstYI Bsu36I BtgI BtgZI EaeI EarI Eco57MI HaeII
Hin4I MfeI MslI MspA1I NspI PasI PmlI PstI
PvuII SapI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI