(Linear) (Six-base) MAPSORT of: KIEE2287.seq  Check: 1171  from: 1  to: 213



KIEE2287.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      60     213 
   Size:         60     153
 
BanII G_rGCy'C

Cuts at:      0      92     213 
   Size:         92     121
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153     213 
   Size:        153      60
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     148     213 
   Size:        148      65
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     213 
   Size:        106     107
 
BseYI C'CCAG_C

Cuts at:      0     170     213 
   Size:        170      43
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     213 
   Size:         92     121
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     213 
   Size:         68     145
 
BsrBI CCG'CTC

Cuts at:      0      99     213 
   Size:         99     114
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     213 
   Size:        106     107
 
HaeII r_GCGC'y

Cuts at:      0      16      76     213 
   Size:         16      60     137

  Fragments arranged by size: 

                 137      60      16
 
NspI r_CATG'y

Cuts at:      0      84     213 
   Size:         84     129
 
PasI CC'CwG_GG

Cuts at:      0      92     213 
   Size:         92     121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     135     213 
   Size:        135      78
 

 Enzymes that do cut:

    AlwNI    BanII     BbsI    BciVI     BpmI    BseYI Bsp1286I    BspMI
    BsrBI  Eco57MI    HaeII     NspI     PasI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI