(Linear) (Six-base) MAPSORT of: KIEE2282.seq  Check: 2566  from: 1  to: 393



KIEE2282.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     393 
   Size:         45     348
 
AflIII A'CryG_T

Cuts at:      0     308     393 
   Size:        308      85
 
AleI CACnn'nnGTG

Cuts at:      0     251     393 
   Size:        251     142
 
ApaI G_GGCC'C

Cuts at:      0      52     393 
   Size:         52     341
 
AvaI C'yCGr_G

Cuts at:      0      44     393 
   Size:         44     349
 
BanII G_rGCy'C

Cuts at:      0      52      96     393 
   Size:         52      44     297

  Fragments arranged by size: 

                 297      52      44
 
BglI GCCn_nnn'nGGC

Cuts at:      0      56     393 
   Size:         56     337
 
Bme1580I G_kGCm'C

Cuts at:      0      52     174     393 
   Size:         52     122     219

  Fragments arranged by size: 

                 219     122      52
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     120     125     393 
   Size:        120       5     268

  Fragments arranged by size: 

                 268     120       5
 
BsaAI yAC'GTr

Cuts at:      0     309     393 
   Size:        309      84
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     393 
   Size:         35     358
 
Bsp1286I G_dGCh'C

Cuts at:      0      35      52      96     174     393 
   Size:         35      17      44      78     219

  Fragments arranged by size: 

                 219      78      44      35      17
 
BssSI C'ACGA_G

Cuts at:      0     173     247     393 
   Size:        173      74     146

  Fragments arranged by size: 

                 173     146      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     393 
   Size:        288     105
 
BtgI C'CryG_G

Cuts at:      0      66     215     393 
   Size:         66     149     178

  Fragments arranged by size: 

                 178     149      66
 
EaeI y'GGCC_r

Cuts at:      0     212     263     296     393 
   Size:        212      51      33      97

  Fragments arranged by size: 

                 212      97      51      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     393 
   Size:         45     348
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48      49     393 
   Size:         48       1     344

  Fragments arranged by size: 

                 344      48       1
 
HaeII r_GCGC'y

Cuts at:      0     352     393 
   Size:        352      41
 
MscI TGG'CCA

Cuts at:      0     214     298     393 
   Size:        214      84      95

  Fragments arranged by size: 

                 214      95      84
 
MslI CAynn'nnrTG

Cuts at:      0      22     251     334     393 
   Size:         22     229      83      59

  Fragments arranged by size: 

                 229      83      59      22
 
MspA1I CmG'CkG

Cuts at:      0     168     318     393 
   Size:        168     150      75

  Fragments arranged by size: 

                 168     150      75
 
NcoI C'CATG_G

Cuts at:      0     215     393 
   Size:        215     178
 
PfoI T'CCnGG_A

Cuts at:      0     122     393 
   Size:        122     271
 
PspOMI G'GGCC_C

Cuts at:      0      48     393 
   Size:         48     345
 
PstI C_TGCA'G

Cuts at:      0     323     393 
   Size:        323      70
 
PvuII CAG'CTG

Cuts at:      0     318     393 
   Size:        318      75
 
SfcI C'TryA_G

Cuts at:      0     319     393 
   Size:        319      74
 
SmaI CCC'GGG

Cuts at:      0      46     393 
   Size:         46     347
 
SmlI C'TyrA_G

Cuts at:      0      99     140     393 
   Size:         99      41     253

  Fragments arranged by size: 

                 253      99      41
 
StyI C'CwwG_G

Cuts at:      0     215     393 
   Size:        215     178
 
XmaI C'CCGG_G

Cuts at:      0      44     393 
   Size:         44     349
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApaI     AvaI    BanII     BglI Bme1580I
    BpuEI    BsaAI  BsiHKAI Bsp1286I    BssSI    BstXI     BtgI     EaeI
  Eco57MI EcoO109I    HaeII     MscI     MslI   MspA1I     NcoI     PfoI
   PspOMI     PstI    PvuII     SfcI     SmaI     SmlI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI