Restriction map for
pFN21AE2281
(Linear) (Six-base) MAPSORT of: KIEE2281.seq Check: 9084 from: 1 to: 2529
KIEE2281.orf
With 182 enzymes: SgfI *
March 22, 2012 16:01 ..
AccI GT'mk_AC
Cuts at: 0 425 743 2529
Size: 425 318 1786
Fragments arranged by size:
1786 425 318
Acc65I G'GTAC_C
Cuts at: 0 820 2529
Size: 820 1709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1687 1864 2529
Size: 1687 177 665
Fragments arranged by size:
1687 665 177
AfeI AGC'GCT
Cuts at: 0 1888 2529
Size: 1888 641
AgeI A'CCGG_T
Cuts at: 0 2029 2529
Size: 2029 500
AhdI GACnn_n'nnGTC
Cuts at: 0 1407 2529
Size: 1407 1122
AleI CACnn'nnGTG
Cuts at: 0 2282 2529
Size: 2282 247
AlwNI CAG_nnn'CTG
Cuts at: 0 546 1501 1785 2529
Size: 546 955 284 744
Fragments arranged by size:
955 744 546 284
ApaLI G'TGCA_C
Cuts at: 0 2518 2529
Size: 2518 11
BamHI G'GATC_C
Cuts at: 0 528 1298 2529
Size: 528 770 1231
Fragments arranged by size:
1231 770 528
BanI G'GyrC_C
Cuts at: 0 820 2284 2529
Size: 820 1464 245
Fragments arranged by size:
1464 820 245
BanII G_rGCy'C
Cuts at: 0 1373 2345 2513 2529
Size: 1373 972 168 16
Fragments arranged by size:
1373 972 168 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 440 2529
Size: 440 2089
BbvCI CC'TCA_GC
Cuts at: 0 952 1356 1904 2529
Size: 952 404 548 625
Fragments arranged by size:
952 625 548 404
BclI T'GATC_A
Cuts at: 0 782 1062 2529
Size: 782 280 1467
Fragments arranged by size:
1467 782 280
BfrBI ATG'CAT
Cuts at: 0 813 2529
Size: 813 1716
BglII A'GATC_T
Cuts at: 0 72 414 2529
Size: 72 342 2115
Fragments arranged by size:
2115 342 72
Bme1580I G_kGCm'C
Cuts at: 0 2203 2522 2529
Size: 2203 319 7
Fragments arranged by size:
2203 319 7
BmgBI CAC'GTC
Cuts at: 0 894 2529
Size: 894 1635
BmrI ACTGGGnnnn_n'
Cuts at: 0 1525 2529
Size: 1525 1004
BmtI G_CTAG'C
Cuts at: 0 67 2529
Size: 67 2462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 232 294 587 720 1382 1412 1616
Size: 171 61 62 293 133 662 30 204
Cuts at: 1616 1663 1970 2266 2387 2529
Size: 47 307 296 121 142
Fragments arranged by size:
662 307 296 293 204 171 142 133
121 62 61 47 30
Bpu10I CC'TnA_GC
Cuts at: 0 916 952 1356 1786 1904 2348 2529
Size: 916 36 404 430 118 444 181
Fragments arranged by size:
916 444 430 404 181 118 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1534 2529
Size: 780 754 995
Fragments arranged by size:
995 780 754
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 2377 2529
Size: 452 1925 152
Fragments arranged by size:
1925 452 152
BsaWI w'CCGG_w
Cuts at: 0 961 2029 2499 2529
Size: 961 1068 470 30
Fragments arranged by size:
1068 961 470 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 216 230 246 260 292 322 1807 1837
Size: 216 14 16 14 32 30 1485 30
Cuts at: 1837 2529
Size: 692
Fragments arranged by size:
1485 692 216 32 30 30 16 14
14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 333 874 1316 1705 1921 1973 2496 2499
Size: 333 541 442 389 216 52 523 3
Cuts at: 2499 2529
Size: 30
Fragments arranged by size:
541 523 442 389 333 216 52 30
3
BseYI C'CCAG_C
Cuts at: 0 1020 1193 1334 1699 1728 2037 2529
Size: 1020 173 141 365 29 309 492
Fragments arranged by size:
1020 492 365 309 173 141 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1064 2529
Size: 1064 1465
BsiEI CG_ry'CG
Cuts at: 0 1446 2030 2101 2529
Size: 1446 584 71 428
Fragments arranged by size:
1446 584 428 71
BsiHKAI G_wGCw'C
Cuts at: 0 377 1055 2513 2522 2529
Size: 377 678 1458 9 7
Fragments arranged by size:
1458 678 377 9 7
BsmI GAATG_Cn'
Cuts at: 0 108 815 1790 2529
Size: 108 707 975 739
Fragments arranged by size:
975 739 707 108
Bsp1286I G_dGCh'C
Cuts at: 0 377 1055 1373 2203 2345 2513 2522 2529
Size: 377 678 318 830 142 168 9 7
Fragments arranged by size:
830 678 377 318 168 142 9 7
BspEI T'CCGG_A
Cuts at: 0 961 2529
Size: 961 1568
BspHI T'CATG_A
Cuts at: 0 1955 2529
Size: 1955 574
BsrBI CCG'CTC
Cuts at: 0 1040 2493 2529
Size: 1040 1453 36
Fragments arranged by size:
1453 1040 36
BsrDI GCAATG_nn'
Cuts at: 0 375 2060 2334 2529
Size: 375 1685 274 195
Fragments arranged by size:
1685 375 274 195
BsrFI r'CCGG_y
Cuts at: 0 1445 2029 2529
Size: 1445 584 500
Fragments arranged by size:
1445 584 500
BssSI C'ACGA_G
Cuts at: 0 1084 2529
Size: 1084 1445
BstAPI GCAn_nnn'nTGC
Cuts at: 0 477 1272 2529
Size: 477 795 1257
Fragments arranged by size:
1257 795 477
BstEII G'GTnAC_C
Cuts at: 0 1852 2529
Size: 1852 677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2055 2360 2529
Size: 2055 305 169
Fragments arranged by size:
2055 305 169
BstYI r'GATC_y
Cuts at: 0 72 414 528 1298 2529
Size: 72 342 114 770 1231
Fragments arranged by size:
1231 770 342 114 72
BstZ17I GTA'TAC
Cuts at: 0 426 744 2529
Size: 426 318 1785
Fragments arranged by size:
1785 426 318
BtgI C'CryG_G
Cuts at: 0 1091 2529
Size: 1091 1438
BtsI GCAGTG_nn'
Cuts at: 0 2118 2529
Size: 2118 411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 822 857 2529
Size: 822 35 1672
Fragments arranged by size:
1672 822 35
EaeI y'GGCC_r
Cuts at: 0 1232 1443 2098 2529
Size: 1232 211 655 431
Fragments arranged by size:
1232 655 431 211
EagI C'GGCC_G
Cuts at: 0 1443 2098 2529
Size: 1443 655 431
Fragments arranged by size:
1443 655 431
EarI CTCTTCn'nnn_
Cuts at: 0 878 1483 1663 2012 2529
Size: 878 605 180 349 517
Fragments arranged by size:
878 605 517 349 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1464 2126 2529
Size: 1464 662 403
Fragments arranged by size:
1464 662 403
EcoICRI GAG'CTC
Cuts at: 0 2511 2529
Size: 2511 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 232 294 587 720 1382 1412 1616
Size: 171 61 62 293 133 662 30 204
Cuts at: 1616 1663 1687 1864 1970 2266 2387 2529
Size: 47 24 177 106 296 121 142
Fragments arranged by size:
662 296 293 204 177 171 142 133
121 106 62 61 47 30 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 200 540 2529
Size: 200 340 1989
Fragments arranged by size:
1989 340 200
EcoO109I rG'GnC_Cy
Cuts at: 0 191 1017 1566 2166 2379 2529
Size: 191 826 549 600 213 150
Fragments arranged by size:
826 600 549 213 191 150
HaeII r_GCGC'y
Cuts at: 0 699 1890 2529
Size: 699 1191 639
Fragments arranged by size:
1191 699 639
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 513 520 545 552 1360 1392
Size: 230 32 251 7 25 7 808 32
Cuts at: 1392 1393 1425 2529
Size: 1 32 1104
Fragments arranged by size:
1104 808 251 230 32 32 32 25
7 7 1
HindIII A'AGCT_T
Cuts at: 0 644 787 2529
Size: 644 143 1742
Fragments arranged by size:
1742 644 143
KpnI G_GTAC'C
Cuts at: 0 824 2529
Size: 824 1705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1815 2529
Size: 1815 714
MscI TGG'CCA
Cuts at: 0 1234 2529
Size: 1234 1295
MslI CAynn'nnrTG
Cuts at: 0 1288 2282 2529
Size: 1288 994 247
Fragments arranged by size:
1288 994 247
MspA1I CmG'CkG
Cuts at: 0 252 1360 1628 1699 1732 2529
Size: 252 1108 268 71 33 797
Fragments arranged by size:
1108 797 268 252 71 33
NaeI GCC'GGC
Cuts at: 0 1447 2529
Size: 1447 1082
NcoI C'CATG_G
Cuts at: 0 1091 2529
Size: 1091 1438
NdeI CA'TA_TG
Cuts at: 0 2178 2529
Size: 2178 351
NgoMIV G'CCGG_C
Cuts at: 0 1445 2529
Size: 1445 1084
NheI G'CTAG_C
Cuts at: 0 63 2529
Size: 63 2466
NsiI A_TGCA'T
Cuts at: 0 815 2529
Size: 815 1714
NspI r_CATG'y
Cuts at: 0 592 2529
Size: 592 1937
PasI CC'CwG_GG
Cuts at: 0 2077 2529
Size: 2077 452
PfoI T'CCnGG_A
Cuts at: 0 187 2529
Size: 187 2342
PpuMI rG'GwC_Cy
Cuts at: 0 191 1017 2529
Size: 191 826 1512
Fragments arranged by size:
1512 826 191
PstI C_TGCA'G
Cuts at: 0 2422 2529
Size: 2422 107
PvuII CAG'CTG
Cuts at: 0 1360 1699 1732 2529
Size: 1360 339 33 797
Fragments arranged by size:
1360 797 339 33
SacI G_AGCT'C
Cuts at: 0 2513 2529
Size: 2513 16
SapI GCTCTTCn'nnn_
Cuts at: 0 1663 2012 2529
Size: 1663 349 517
Fragments arranged by size:
1663 517 349
SfcI C'TryA_G
Cuts at: 0 2322 2334 2418 2529
Size: 2322 12 84 111
Fragments arranged by size:
2322 111 84 12
SmlI C'TyrA_G
Cuts at: 0 795 1549 2529
Size: 795 754 980
Fragments arranged by size:
980 795 754
SphI G_CATG'C
Cuts at: 0 592 2529
Size: 592 1937
StyI C'CwwG_G
Cuts at: 0 488 600 1091 1112 1535 2529
Size: 488 112 491 21 423 994
Fragments arranged by size:
994 491 488 423 112 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 507 2529
Size: 507 2022
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 2529
Size: 292 32 2205
Fragments arranged by size:
2205 292 32
Tth111I GACn'n_nGTC
Cuts at: 0 1222 2529
Size: 1222 1307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 933 2266 2529
Size: 933 1333 263
Fragments arranged by size:
1333 933 263
XmnI GAAnn'nnTTC
Cuts at: 0 887 2529
Size: 887 1642
Enzymes that do cut:
AccI Acc65I AcuI AfeI AgeI AhdI AleI AlwNI
ApaLI BamHI BanI BanII BbsI BbvCI BclI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PfoI PpuMI PstI PvuII SacI SapI SfcI SmlI
SphI StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AlfI AloI ApaI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BcgI BciVI BglI BlpI BplI BsaAI BsaBI BsaHI
BsiWI BsmBI BspMI BsrGI BssHII BstBI Bsu36I BtgZI
ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI MfeI MluI NarI
NotI NruI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TatI XbaI XhoI XmaI ZraI