Restriction map for
pFN21AE2280
(Linear) (Six-base) MAPSORT of: KIEE2280.seq Check: 2603 from: 1 to: 1218
KIEE2280.orf
With 182 enzymes: SgfI *
July 15, 2010 18:56 ..
AccI GT'mk_AC
Cuts at: 0 281 1218
Size: 281 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1218
Size: 463 755
AflIII A'CryG_T
Cuts at: 0 509 1218
Size: 509 709
AlwNI CAG_nnn'CTG
Cuts at: 0 915 1218
Size: 915 303
ApaI G_GGCC'C
Cuts at: 0 8 1029 1218
Size: 8 1021 189
Fragments arranged by size:
1021 189 8
ApoI r'AATT_y
Cuts at: 0 727 1218
Size: 727 491
AvaI C'yCGr_G
Cuts at: 0 596 866 1136 1218
Size: 596 270 270 82
Fragments arranged by size:
596 270 270 82
BamHI G'GATC_C
Cuts at: 0 480 1218
Size: 480 738
BanI G'GyrC_C
Cuts at: 0 606 1057 1108 1218
Size: 606 451 51 110
Fragments arranged by size:
606 451 110 51
BanII G_rGCy'C
Cuts at: 0 8 1029 1218
Size: 8 1021 189
Fragments arranged by size:
1021 189 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 687 1218
Size: 537 150 531
Fragments arranged by size:
537 531 150
BbvCI CC'TCA_GC
Cuts at: 0 962 1218
Size: 962 256
BglI GCCn_nnn'nGGC
Cuts at: 0 1024 1218
Size: 1024 194
BglII A'GATC_T
Cuts at: 0 503 1218
Size: 503 715
BlpI GC'TnA_GC
Cuts at: 0 158 1218
Size: 158 1060
Bme1580I G_kGCm'C
Cuts at: 0 8 611 1029 1113 1218
Size: 8 603 418 84 105
Fragments arranged by size:
603 418 105 84 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 496 1218
Size: 120 376 722
Fragments arranged by size:
722 376 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 289 310 362 1210 1218
Size: 217 72 21 52 848 8
Fragments arranged by size:
848 217 72 52 21 8
Bpu10I CC'TnA_GC
Cuts at: 0 962 1218
Size: 962 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1218
Size: 404 814
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 1218
Size: 286 932
BsaAI yAC'GTr
Cuts at: 0 510 1218
Size: 510 708
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 1218
Size: 232 30 956
Fragments arranged by size:
956 232 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1218
Size: 10 1208
BseYI C'CCAG_C
Cuts at: 0 7 303 459 771 1218
Size: 7 296 156 312 447
Fragments arranged by size:
447 312 296 156 7
BsiHKAI G_wGCw'C
Cuts at: 0 1042 1218
Size: 1042 176
BsmI GAATG_Cn'
Cuts at: 0 963 1218
Size: 963 255
Bsp1286I G_dGCh'C
Cuts at: 0 8 611 1029 1042 1113 1218
Size: 8 603 418 13 71 105
Fragments arranged by size:
603 418 105 71 13 8
BsrBI CCG'CTC
Cuts at: 0 216 273 1218
Size: 216 57 945
Fragments arranged by size:
945 216 57
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1037 1218
Size: 1037 181
BstYI r'GATC_y
Cuts at: 0 480 503 903 1218
Size: 480 23 400 315
Fragments arranged by size:
480 400 315 23
BtgI C'CryG_G
Cuts at: 0 575 1218
Size: 575 643
BtsI GCAGTG_nn'
Cuts at: 0 190 937 1218
Size: 190 747 281
Fragments arranged by size:
747 281 190
DraIII CAC_nnn'GTG
Cuts at: 0 78 1218
Size: 78 1140
EaeI y'GGCC_r
Cuts at: 0 893 1218
Size: 893 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 289 310 362 463 1210 1218
Size: 217 72 21 52 101 747 8
Fragments arranged by size:
747 217 101 72 52 21 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 689 1218
Size: 689 529
EcoO109I rG'GnC_Cy
Cuts at: 0 4 49 346 986 1218
Size: 4 45 297 640 232
Fragments arranged by size:
640 297 232 45 4
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 246 264 278 465 497 1218
Size: 232 14 18 14 187 32 721
Fragments arranged by size:
721 232 187 32 18 14 14
HindIII A'AGCT_T
Cuts at: 0 590 1218
Size: 590 628
MscI TGG'CCA
Cuts at: 0 895 1218
Size: 895 323
MspA1I CmG'CkG
Cuts at: 0 463 636 832 1055 1218
Size: 463 173 196 223 163
Fragments arranged by size:
463 223 196 173 163
NcoI C'CATG_G
Cuts at: 0 575 1218
Size: 575 643
NspI r_CATG'y
Cuts at: 0 154 1218
Size: 154 1064
PasI CC'CwG_GG
Cuts at: 0 349 617 1218
Size: 349 268 601
Fragments arranged by size:
601 349 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 1218
Size: 78 1140
PfoI T'CCnGG_A
Cuts at: 0 250 675 1218
Size: 250 425 543
Fragments arranged by size:
543 425 250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1133 1165 1218
Size: 1133 32 53
Fragments arranged by size:
1133 53 32
PpuMI rG'GwC_Cy
Cuts at: 0 49 986 1218
Size: 49 937 232
Fragments arranged by size:
937 232 49
PspOMI G'GGCC_C
Cuts at: 0 4 1025 1218
Size: 4 1021 193
Fragments arranged by size:
1021 193 4
PvuII CAG'CTG
Cuts at: 0 463 636 832 1218
Size: 463 173 196 386
Fragments arranged by size:
463 386 196 173
SanDI GG'GwC_CC
Cuts at: 0 49 986 1218
Size: 49 937 232
Fragments arranged by size:
937 232 49
SexAI A'CCwGG_T
Cuts at: 0 684 1218
Size: 684 534
SmaI CCC'GGG
Cuts at: 0 868 1218
Size: 868 350
SmlI C'TyrA_G
Cuts at: 0 419 1218
Size: 419 799
SphI G_CATG'C
Cuts at: 0 154 1218
Size: 154 1064
StuI AGG'CCT
Cuts at: 0 672 1218
Size: 672 546
StyI C'CwwG_G
Cuts at: 0 57 476 550 575 1218
Size: 57 419 74 25 643
Fragments arranged by size:
643 419 74 57 25
TatI w'GTAC_w
Cuts at: 0 83 1218
Size: 83 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 930 962 1218
Size: 930 32 256
Fragments arranged by size:
930 256 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 467 483 1218
Size: 467 16 735
Fragments arranged by size:
735 467 16
XmaI C'CCGG_G
Cuts at: 0 866 1218
Size: 866 352
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BsrBI BstAPI BstYI BtgI
BtsI DraIII EaeI Eco57MI EcoNI EcoO109I Hin4I HindIII
MscI MspA1I NcoI NspI PasI PflMI PfoI PpiI
PpuMI PspOMI PvuII SanDI SexAI SmaI SmlI SphI
StuI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI